Kent SJ, Dale CJ, Rose RD, Medveczky CJ, Ramshaw IA; International Conference on AIDS.
Int Conf AIDS. 2002 Jul 7-12; 14: abstract no. MoOrA1090.
University of Melbourne, Parkville, Australia
BACKGROUND: Detection of HIV-specific T cell immunity induced by vaccines has been, up to date, at best indirect. We studied the direct killing of HIV labeled cells in vivo tracking dye-labeled cells in macaques receiving highly T cell immunogenic vaccines. METHODS: Macaques were vaccinated consecutively with DNA and Fowlpoxvirus HIV-1 vaccines as previously reported (Kent et al J Virol 1998). 107 fresh autologous PBMC were first labeled with the vital dye SNARF and then pulsed with overlapping 15mer HIV-1gag peptides. Together with an equal number of non-peptide pulsed CSFE dye-labeled control PBMC, the SNARF-labeled HIV pulsed PBMC were infused into macaques the same day and followed in blood and lymph nodes over 1 week. RESULTS: SNARF and CFSE-labeled macaque PBMC and lymph node cells could be readily recovered in control experiments for at least 1 week following infusion. In excess of 50% of the HIV-pulsed SNARF-labeled cells were killed, both in blood and lymph nodes, within 1-2 days of infusion in DNA/FPV immunized animals in comparison to non-HIV pulsed CFSE-labeled cells. Control macaques not receiving peptide pulsed cells did not have significant loss of SNARF-labeled cells and macaques previously infected with non-pathogenic HIV-1 demonstrated less in vivo killing of HIV-pulsed SNARF-labeled cells in comparison to DNA/FPV vaccinated macaques. CONCLUSION: This is the first demonstration of the direct in vivo killing, both in blood and lymph nodes, of HIV labeled cells in primates. The data dramatically illustrate the high T cell immunity induced by DNA/FPV prime/boost HIV-1 vaccines in primates. DNA and FPV vaccine regimens should be readily capable of facilitating clearance of HIV-1 infected cells in humans exposed to HIV-1.
Publication Types:
Keywords:
- AIDS Vaccines
- Acquired Immunodeficiency Syndrome
- Animals
- DNA
- DNA, Viral
- Fowlpox virus
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- HIV-1
- Homicide
- Humans
- Macaca
- Resin Cements
- T-Lymphocytes
- Vaccines, DNA
- immunology
Other ID:
UI: 102251465
From Meeting Abstracts