De Lange GG, Weinberg AE, Scherpbier HJ, Mulder G, Boer K, Weening RS; International Conference on AIDS.

Int Conf AIDS. 1992 Jul 19-24; 8: B204 (abstract no. PoB 3682).

Central Lab of the NRC Blood Transf. Service, Amsterdam, The Netherlands.

OBJECTIVES: To diagnose HIV infection in children born to HIV-seropositive mothers immunoglobulin (Ig) allotyping was used. At birth serum of the infant will show the IgG phenotype of the mother and will have her anti-HIV antibodies both due to placental transfer of IgG. Early after birth the child will start to produce its own IgG, which may show another IgG phenotype within some months. The aim of the study was to diagnose vertical HIV-transmission by using difference in IgG phenotype to discriminate between self-produced and maternal anti-HIV antibodies. METHODS: Twenty-two women, found to be HIV-seropositive during pregnancy and their infants were typed for Ig allotypes by haemagglutination-inhibition-assay. Although in 18 cases a different Gm and/or Km phenotype could be ascertained, in only 10 of these, this was due to a difference in IgG1 namely the allotypes Glm(z), (a) and/or (f). Since anti-HIV antibodies are mainly IgG1, radio-immuno-assays (RIA) were developed to determine the IgG1 isotype and Glm allotypes of anti-HIV antibodies in a serum sample of the mother and several follow-up samples of the child. RESULTS: Eight cases with a Glm difference between mother and child and 5 other cases were investigated for the IgG1 isotype and Glm allotypes of anti-HIV antibodies by RIA. In 2 cases production of anti-HIV antibodies by the child could be proven, which was in agreement with the clinical outcome. Of the other 11 children 10 sero-reverted and one had a decreasing level of IgG anti-HIV antibodies. No HIV-related clinical symptoms were seen in these children, which have been followed for 11-46 months. CONCLUSIONS: Assays could be developed to distinguish between maternal and self-produced IgG anti-HIV antibodies in children born to HIV-seropositive mothers, which are based on differences in Gm allotypes. The results were in agreement with the clinical outcome.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Child
• Disease Transmission, Vertical
• Female
• HIV Antibodies
• HIV Infections
• HIV Seropositivity
• Humans
• Immunoglobulin G
• Infant
• Infant, Newborn
• Mothers
• Pregnancy
• transmission

Other ID:

• 92401407

UI: 102199120

From Meeting Abstracts