Mead JR, You XD; Keystone Symposia on Molecular and Cellular Biology:1998 Opportunistic Infections in AIDS.
Keyst Symp Mol Cell Biol Keyst Symp Mol Cell Biol. 1998 Apr 2-8; 117 (abstract no. 207).
Emory University, Atlanta, GA.
The mechanisms of immunity to cryptosporidiosis is yet poorly understood. Susceptibility to infection was investigated between 2 strains of gamma interferon knockout mice (GKO). Female C57BL/6J-Ifg and BALB/c-Ifg gamma-interferon knockout mice (GKO), ages 8 to 10 weeks (The Jackson Lab., Bar Harbor, ME) were infected with different infection doses (10(3) - 10(5) oocysts/mouse). Mouse fecal samples were collected every day after infection to determine oocyst shedding. The infection intensity in C57BL/6 GKO mice infected with 1 x 10(5) oocysts/mouse increased markedly on day 4 after infection and continued to increase significantly over the next six to seven days. The average parasite shedding was 332 oocysts per 100 microliter fecal sample day 4 post infection (p.i.) and 36,081 plus or minus 9,590 (SE) oocysts per 100 microliter fecal sample on day 9 after infection. In contrast, parasite load average in the BALB/c GKO was 100 times lower then C57 black 6 mice (350 oocysts per 100 microliter at peak shedding (12 days p.i.). most of the C57BL GKO mice had weight loss of 15% or more and died by day 10 p.i.. There was little difference in survival of mice inoculated with different doses. In contrast, BALB/c GKO mice survived until termination of the experiment (39 days) even when 1 x 10(6) oocysts per mouse were used for inoculation. Parasite load decreased markedly by 30 days p.i. (46 oocysts per 100 microliter). No weight loss was observed or difference in stool consistency was observed. Additionally, immune response to whole soluble and fractionated cryptosporidial antigen was observed 39 days p.i.
Publication Types:
Keywords:
- Animals
- Communicable Diseases
- Cryptosporidiosis
- Cryptosporidium parvum
- Disease Susceptibility
- Feces
- Female
- Humans
- Infection
- Interferon Type II
- Interferon-gamma, Recombinant
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Mice, Knockout
- Oocysts
Other ID:
UI: 102236519
From Meeting Abstracts