Maticic M, Poljak M, Tomazic J, Vidmar L, Kramar B, Skaleric U; International Conference on AIDS.

Int Conf AIDS. 1998; 12: 422 (abstract no. 23401).

Dpt. of Infectious Dis., Univ. Medic. Centre, Ljubljana, Slovenia.

OBJECTIVES: To search for proviral HIV-1 DNA in GF of patients, previously determined as HIV-1 seropositive in various stages of HIV disease and to find some relationship with their general and periodontal clinical, virologic and immunologic status. DESIGN: Prospective, controlled study. METHODS: GF samples from 35 HIV-1 seropositive patients and 10 HIV-1 seronegative controls were evaluated for the presence of proviral HIV-1 DNA. The clinical and immunologic classification of HIV infection, periodontal status, oral lesions and gingival bleeding plasma HIV-1 RNA viral load and antiretroviral drug use were documented in all HIV-1 seropositive patients. GF samples were collected by paper points and analysed by polymerase chain reaction for the presence of HIV-1 DNA using a slightly modified commercial diagnostic test. RESULTS: Out of 35 HIV-1 seropositive patients, expressing most various stages of HIV disease, proviral HIV-1 DNA was detected in 17 who revealed significantly higher plasma HIV-1 RNA viral load compared to the group of 18 HIV-1 positive patients harbouring no HIV-1 DNA in GF samples (p = 0.03). In the group of 17 GF HIV-1 DNA positive patients a significant correlation between the periodontal clinical attachment loss and plasma HIV-1 RNA viral load was noticed (p = 0.002). HIV-1 DNA was found only in GF samples of patients with a peripheral blood CD4+ cell count below 500 x 10(6)/l. Out of 26 GF samples of HIV-1 seropositive patients, containing blood, HIV-1 DNA was detected in 14. In three HIV-1 DNA positive GF samples on blood was found. The antiretroviral therapy, local gingival bleeding and oral lesions had no significant influence on detection of HIV-1 DNA in GF samples. CONCLUSION: Mononuclear cells present in GF harbouring HIV-1 DNA could represent a potential epidemiologic source of HIV-1 infection (i.e. at dental procedures, vigorous kissing) in the presence or absence of local gingival bleeding especially in persons with advanced HIV-1 infection and increased loss of periodontal clinical attachment.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• CD4 Lymphocyte Count
• Disease Transmission, Vertical
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• Longitudinal Studies
• Periodontal Diseases
• Polymerase Chain Reaction
• Prospective Studies
• Viral Load
• reverse transcriptase, Human immunodeficiency virus 1
• transmission

Other ID:

• 98395431

UI: 102229031

From Meeting Abstracts