BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract

Checking for E. coli insertion elements
Checking for E. coli insertion elements
Masked Sequence:
>'E14B05_D05_03.ab1'
CGGCCGGGNATTGGATTGATCAATTAGTAGCAGCCGCAACAGCTTCTTAT
CTCCTTTACTACCTCTCAGGAGGACAGGCAACTCCAGTTCATACGCTGGC
CAGTGGAGTGGGGTATGGTCAAGGAGTAGTTTGGGAGATTGTGTTGACGT
TTTCTTTGTTGTTCACTGTGTATGCGACCATGGTGGATCCCAAGAAGGGA
GCACTTGCTGGGCTTGGGCCAACGCTGGTTGGGTTTGTGGTTGGGGCCAA
CATCCTCGCAGGTGGGGCTTACTCTGCTGCTTCTATGAACCCAGCAAGGT
CTTTTGGGCCCTGCCTTGGTCGCTGGAAACTGGACCGATCATTGGGCCTA
CTGGGTTGGGCCTCTCATTGGTGGTGGCCTTGCTGGTTACATCTATGAGA
CTTTCNTTCATTGACCGATCTCATGTTCCACTTCCTCGTGATGAAGAAAA
CTAAACAATAGGATTGACAACTTGCACTTTCATCCCTTCTTAAAAAATGC
TGCATTATTGTAGTTAATTTAATCTAATAAT


Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice:  statistical significance is estimated under the assumption that the
equivalent of one entire reading frame in the query sequence codes for protein
and that significant alignments will involve only coding reading frames.

Query=  'E14B05_D05_03.ab1'
        (531 letters)
  Translating both strands of query sequence in all 6 reading frames

Database:  nr
           625,274 sequences; 197,782,623 total letters.


     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 7 Sequences     : less than 7 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 2200 389 |=======================================================
   6310 1811 247 |===================================
   3980 1564 333 |===============================================
   2510 1231 205 |=============================
   1580 1026 226 |================================
   1000  800 143 |====================
    631  657  96 |=============
    398  561  61 |========
    251  500  46 |======
    158  454  26 |===
    100  428  23 |===
   63.1  405   9 |=
   39.8  396   9 |=
   25.1  387   5 |:
   15.8  382   7 |=
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 375  <<<<<<<<<<<<<<<<<
   10.0  375  10 |=
   6.31  365   7 |=
   3.98  358   1 |:
   2.51  357   4 |:
   1.58  353   5 |:
   1.00  348   6 |:
   0.63  342   2 |:
   0.40  340   6 |:
   0.25  334   6 |:
   0.16  328   5 |:
   0.10  323   4 |:
  0.063  319   4 |:
  0.040  315   3 |:
  0.025  312   6 |:
  0.016  306   3 |:
  0.010  303   8 |=
 0.0063  295  12 |=
 0.0040  283   6 |:
 0.0025  277  10 |=
 0.0016  267  11 |=


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|3643602|gb|AAC42249.1|(AC005395) putative aquapori... +3   425  2.3e-55   2
gi|7441262|pir||T14843aquaporin-like protein - Norway... +3   313  2.8e-39   2
gi|1145697|gb|AAC49281.1|(U39485) delta tonoplast int... +3   311  2.8e-39   2
gi|4539145|emb|CAB39758.1|(AJ133748) major intrinsic ... +3   303  3.6e-39   2
gi|11276919|pir||T48885water channel protein MipK [im... +3   306  4.6e-39   2
gi|7441258|pir||T07819probable water channel protein ... +3   311  9.5e-39   2
gi|7441281|pir||T12632water channel protein - common ... +3   308  1.2e-38   2
gi|11276918|pir||T48884membrane channel protein [impo... +3   301  1.2e-38   2
gi|7441271|pir||T14001aquaporin TIP18 - common sunflo... +3   320  1.5e-38   2
gi|2058706|gb|AAB53329.1|(U95008) Rb7 [Lycopersicon e... +3   301  1.5e-38   2
gi|7441273|pir||T14002aquaporin TIP7 - common sunflow... +3   317  2.0e-38   2
gi|267135|sp|P24422|TIP2_TOBACTONOPLAST INTRINSIC PRO... +3   301  4.1e-38   2
gi|8699606|gb|AAF78758.1|AF271661_1(AF271661) putativ... +3   309  5.2e-38   2
gi|10047074|emb|CAB95746.2|(AJ289866) putative aquapo... +3   309  5.2e-38   2
gi|7441272|pir||T14000aquaporin TIP7 - common sunflow... +3   305  5.2e-38   2
gi|126959|sp|P21653|TIP1_TOBACTONOPLAST INTRINSIC PRO... +3   300  5.2e-38   2
gi|7441255|pir||T01648probable tonoplast aquaporin - ... +3   303  6.6e-38   2
gi|9623335|gb|AAF90121.1|AF254799_1(AF254799) tonopla... +3   292  1.1e-37   2
gi|4099406|gb|AAD10494.1|(U86762) gamma-type tonoplas... +3   297  1.7e-37   2
gi|3004950|gb|AAC09245.1|(AF037061) tonoplast intrins... +3   294  3.6e-37   2
gi|7441268|pir||T14314probable membrane protein - car... +3   304  4.6e-37   2
gi|629784|pir||S47037tonoplast intrinsic protein gamm... +3   293  4.6e-37   2
gi|11276920|pir||T48886aquaporin [imported] - Vernici... +3   293  4.6e-37   2
gi|7441289|pir||T10804tonoplast intrinsic protein, de... +3   308  5.8e-37   2
gi|12006843|gb|AAG44946.1|AF290619_1(AF290619) putati... +3   287  1.5e-36   2
gi|4138262|emb|CAA69353.1|(Y08161) aquaporin 1 [Nicot... +3   287  1.5e-36   2
gi|12006841|gb|AAG44945.1|AF290618_1(AF290618) putati... +3   295  5.1e-36   2
gi|4884870|gb|AAD31849.1|AF133533_1(AF133533) water c... +3   291  5.1e-36   2
gi|7441256|pir||F71442probable membrane channel prote... +3   296  6.5e-36   2
gi|9758773|dbj|BAB09071.1|(AB025628) membrane channel... +3   293  2.8e-35   2
gi|4099408|gb|AAD10495.1|(U86763) delta-type tonoplas... +3   286  2.8e-35   2
gi|4584429|emb|CAB40742.1|(AJ237751) aquaglyceroporin... +3   382  2.5e-34   1
gi|2246621|gb|AAB62692.1|(AF004393) salt-stress induc... +3   310  1.1e-26   1
gi|9293929|dbj|BAB01832.1|(AB028611) salt-stress indu... +3   310  1.1e-26   1
gi|9988412|dbj|BAB12722.1|(AB048248) gamma tonoplast ... +3   310  1.1e-26   1
gi|1928981|gb|AAB51393.1|(U92651) tonoplast intrinsic... +3   308  1.7e-26   1
gi|8699604|gb|AAF78757.1|AF271660_1(AF271660) putativ... +3   306  2.8e-26   1
gi|5911732|emb|CAB55837.1|(AJ245953) delta tonoplast ... +3   305  3.6e-26   1
gi|135860|sp|P25818|TIPG_ARATHTONOPLAST INTRINSIC PRO... +3   304  4.5e-26   1
gi|475048|emb|CAA51171.1|(X72581) tonoplast intrinsic... +3   304  4.5e-26   1
gi|1514977|dbj|BAA12711.1|(D84669) VM23 [Raphanus sat... +3   304  4.5e-26   1
gi|5081419|gb|AAD39372.1|AF118381_1(AF118381) tonopla... +3   304  4.5e-26   1
gi|7441269|pir||T14960probable membrane intrinsic pro... +3   303  5.8e-26   1
gi|126962|sp|P21652|TIPR_ARATHTONOPLAST INTRINSIC PRO... +3   302  7.4e-26   1
gi|1928983|gb|AAB51394.1|(U92652) tonoplast intrinsic... +3   302  7.4e-26   1
gi|541951|pir||JQ2288SPCP2 protein - soybean >gi|3105... +3   302  7.4e-26   1
gi|7340919|dbj|BAA92991.1|(AP001550) ESTs C91632(E311... +3   299  1.5e-25   1
gi|9082285|gb|AAF82790.1|AF275315_1(AF275315) water-s... +3   297  2.5e-25   1
gi|11357300|pir||T51819gamma tonoplast intrinsic prot... +3   296  3.2e-25   1
gi|1729971|sp|P50156|TIPG_ORYSATONOPLAST INTRINSIC PR... +3   291  1.1e-24   1

Use the  and  icons to retrieve links to Entrez:
E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).

Use the  icon (if present) to retrieve links to the Sequence Retrieval System (SRS).

Use the  icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .

Use the  icon (if present) to retrieve links to the Protein Data Bank database.

WARNING:  Descriptions of 325 database sequences were not reported due to the
          limiting value of parameter V = 50.



 >gi|3643602|gb|AAC42249.1|  (AC005395) putative aquaporin (tonoplast intrinsic
            protein) [Arabidopsis thaliana]
            Length = 249

Frame  3 hits (HSPs):      _______________  ______________________        
Frame  2 hits (HSPs):                                            ________ 
Frame  1 hits (HSPs):                                          _______    
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 249
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 425 (149.6 bits), Expect = 2.3e-55, Sum P(2) = 2.3e-55
 Identities = 80/107 (74%), Positives = 91/107 (85%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WIDQL+A++ A +LL YL+GG  TPVHTLASGV Y QG++WEI+LTFSLLFTVYAT+VDP
Sbjct:   101 WIDQLLASSAACFLLSYLTGGMGTPVHTLASGVSYTQGIIWEIILTFSLLFTVYATIVDP 160

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL--GRW 326
             KKG+L G GP L GFVVGANILAGGA+S ASMNPARSFGP L  G W
Sbjct:   161 KKGSLDGFGPLLTGFVVGANILAGGAFSGASMNPARSFGPALVSGNW 207

 Score = 173 (60.9 bits), Expect = 2.3e-55, Sum P(2) = 2.3e-55
 Identities = 28/31 (90%), Positives = 30/31 (96%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+GNWTDHW YWVGPLIGGGLAG+IYE
Sbjct:   199 GPALVSGNWTDHWVYWVGPLIGGGLAGFIYE 229

 Score = 59 (20.8 bits), Expect = 5.6e-17, Sum P(2) = 5.6e-17
 Identities = 24/71 (33%), Positives = 35/71 (49%), Frame = +3

Query:   120 QGVVWEIVLTFSLLFT-VYATMV-DPKKG-ALAGLGPTLVG--FVVGANILAGGAYSAAS 284
             + ++ E + TF  +F  V + M  D   G  L GL    V   FVV A +++ G  S   
Sbjct:    19 KALIVEFITTFLFVFAGVGSAMATDSLVGNTLVGLFAVAVAHAFVV-AVMISAGHISGGH 77

Query:   285 MNPARSFGPCLG 320
             +NPA + G  LG
Sbjct:    78 LNPAVTLGLLLG 89

 Score = 52 (18.3 bits), Expect = 1.2e-42, Sum P(2) = 1.2e-42
 Identities = 17/48 (35%), Positives = 24/48 (50%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLPRDEE 448
             W   W    GP+IG  G GL+     +  ++    IDR HVP+  DE+
Sbjct:   207 WTDHWVYWVGPLIG--G-GLA---GFIYENV---LIDRPHVPVADDEQ 245


 >gi|7441262|pir||T14843  aquaporin-like protein - Norway spruce
            >gi|3021538|emb|CAA06335.1| (AJ005078) aquaporin-like protein
            [Picea abies]
            Length = 252

Frame  3 hits (HSPs):                        _____________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 252
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 313 (110.2 bits), Expect = 2.8e-39, Sum P(2) = 2.8e-39
 Identities = 58/102 (56%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ A  A  LL + +GG +TP+H++ASG+  G+GVV EIV+TF+L++TVYAT  DP
Sbjct:   107 WIAQLLGAIVACLLLKFTTGGLSTPIHSVASGMNTGEGVVMEIVITFALIYTVYATAADP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG L  + P  +GF+VGANILA G +S  SMNPARSF P +
Sbjct:   167 KKGDLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFDPAV 208

 Score = 132 (46.5 bits), Expect = 2.8e-39, Sum P(2) = 2.8e-39
 Identities = 21/29 (72%), Positives = 27/29 (93%), Frame = +1

Query:   310 PALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             PA+V+G++TD+W YWVGPLIGGGLAG +Y
Sbjct:   206 PAVVSGDFTDNWVYWVGPLIGGGLAGIVY 234


 >gi|1145697|gb|AAC49281.1|  (U39485) delta tonoplast integral protein
            [Arabidopsis thaliana] >gi|9279707|dbj|BAB01264.1| (AB023046) delta
            tonoplast intrinsic protein [Arabidopsis thaliana]
            Length = 250

Frame  3 hits (HSPs):               ______________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 311 (109.5 bits), Expect = 2.8e-39, Sum P(2) = 2.8e-39
 Identities = 56/102 (54%), Positives = 77/102 (75%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL Y++GG A P H++A+G+G  +GVV EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSTAACFLLKYVTGGLAVPTHSVAAGLGSIEGVVMEIIITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +G +VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 134 (47.2 bits), Expect = 2.8e-39, Sum P(2) = 2.8e-39
 Identities = 22/30 (73%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ AG+++ HW YWVGPLIGGGLAG IY
Sbjct:   203 GPAVAAGDFSGHWVYWVGPLIGGGLAGLIY 232

 Score = 47 (16.5 bits), Expect = 4.4e-11, Sum P(2) = 4.4e-11
 Identities = 10/30 (33%), Positives = 15/30 (50%), Frame = +3

Query:   231 GFVVGANILAGGAYSAASMNPARSFGPCLG 320
             GF +   +  G   S   +NPA +FG  +G
Sbjct:    64 GFALFVAVAIGANISGGHVNPAVTFGLAVG 93

 Score = 44 (15.5 bits), Expect = 9.1e-11, Sum P(2) = 9.1e-11
 Identities = 29/94 (30%), Positives = 39/94 (41%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQ-----GVV-WEIVL---TFSLLFTVYAT--MVDPKKGALA 209
             +SGG   P  T    VG GQ     GV  W   L   T +     Y T  +  P     A
Sbjct:    77 ISGGHVNPAVTFGLAVG-GQITVITGVFYWIAQLLGSTAACFLLKYVTGGLAVPTHSVAA 135

Query:   210 GLGPTLVGFVVGANILAGGAYS--AASMNPAR-SFG 308
             GLG ++ G V+   I     Y+  A + +P + S G
Sbjct:   136 GLG-SIEGVVMEIIITFALVYTVYATAADPKKGSLG 170


 >gi|4539145|emb|CAB39758.1|  (AJ133748) major intrinsic protein [Picea abies]
            Length = 253

Frame  3 hits (HSPs):                       _______________________       
Frame  2 hits (HSPs):                                            _________
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 303 (106.7 bits), Expect = 3.6e-39, Sum P(2) = 3.6e-39
 Identities = 58/108 (53%), Positives = 78/108 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ A  AS LL + + G++T    ++SGVG    VV EIV+TF L++TVYAT +D 
Sbjct:   107 WIAQLLGAVVASLLLKFTTNGRSTSPFAVSSGVGSWNAVVLEIVMTFGLVYTVYATAIDA 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL--GRWK 329
             K+G+L  + P  +GF+VGANILAGGA+  ASMNPAR+FGP L  G+W+
Sbjct:   167 KRGSLGTIAPLAIGFIVGANILAGGAFDGASMNPARAFGPALVSGKWR 214

 Score = 141 (49.6 bits), Expect = 3.6e-39, Sum P(2) = 3.6e-39
 Identities = 23/31 (74%), Positives = 25/31 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+G W  HW YWVGPLIGGG AG +YE
Sbjct:   205 GPALVSGKWRYHWIYWVGPLIGGGFAGLLYE 235

 Score = 43 (15.1 bits), Expect = 6.9e-29, Sum P(2) = 6.9e-29
 Identities = 18/46 (39%), Positives = 20/46 (43%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPL-PRD 442
             W   W    GP+IG    GL L   L+V S  L      H PL P D
Sbjct:   213 WRYHWIYWVGPLIGGGFAGL-LYEWLMVPSEPL------HQPLQPED 252


 >gi|11276919|pir||T48885  water channel protein MipK [imported] - common ice
            plant >gi|4884868|gb|AAD31848.1|AF133532_1 (AF133532) water channel
            protein MipK [Mesembryanthemum crystallinum]
            Length = 248

Frame  3 hits (HSPs):                  ____ ______________________        
Frame  1 hits (HSPs):                                           ________  
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 306 (107.7 bits), Expect = 4.6e-39, Sum P(2) = 4.6e-39
 Identities = 56/102 (54%), Positives = 75/102 (73%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL  +    +LL   +GG  TP+H++ASGVG  + VV EI++TF+L++TVYAT VDP
Sbjct:   105 WIAQLAGSVAGCFLLKAATGGLTTPIHSVASGVGAIEAVVMEIIITFALVYTVYATAVDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             K+G++  + P  +GF+VGANILA G +S  SMNPARSFGP L
Sbjct:   165 KRGSVGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAL 206

 Score = 137 (48.2 bits), Expect = 4.6e-39, Sum P(2) = 4.6e-39
 Identities = 24/36 (66%), Positives = 27/36 (75%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYET-FXH 411
             GPAL +G++ DHW YWVGPLIGGGLAG  Y   F H
Sbjct:   203 GPALASGDFHDHWVYWVGPLIGGGLAGLTYSNVFMH 238

 Score = 37 (13.0 bits), Expect = 2.0e-10, Sum P(2) = 2.0e-10
 Identities = 9/20 (45%), Positives = 10/20 (50%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQ 122
             +SGG   P  T    VG GQ
Sbjct:    77 ISGGHVNPAVTFGLAVG-GQ 95


 >gi|7441258|pir||T07819  probable water channel protein delta-VM23 - radish
            >gi|3298327|dbj|BAA31452.1| (AB010416) delta-VM23 [Raphanus
            sativus]
            Length = 248

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 311 (109.5 bits), Expect = 9.5e-39, Sum P(2) = 9.5e-39
 Identities = 56/102 (54%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ +  A +LL Y++GG A P H++A+GVG  +GVV EI++TFSL++TVY T  DP
Sbjct:   105 WVAQLLGSTAACFLLKYVTGGLAVPTHSVAAGVGAIEGVVMEIIITFSLVYTVYPTAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +G +VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 129 (45.4 bits), Expect = 9.5e-39, Sum P(2) = 9.5e-39
 Identities = 21/30 (70%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ AG+++ HW YWVGPLIGGGLAG  Y
Sbjct:   203 GPAVAAGDFSGHWVYWVGPLIGGGLAGVTY 232

 Score = 39 (13.7 bits), Expect = 1.1e-09, Sum P(2) = 1.1e-09
 Identities = 22/69 (31%), Positives = 28/69 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQ-----GVV-WEIVL---TFSLLFTVYAT--MVDPKKGALA 209
             +SGG   P  T    VG GQ     GV  W   L   T +     Y T  +  P     A
Sbjct:    77 ISGGHVNPAVTFGLAVG-GQITLITGVFYWVAQLLGSTAACFLLKYVTGGLAVPTHSVAA 135

Query:   210 GLGPTLVGFVV 242
             G+G  + G V+
Sbjct:   136 GVG-AIEGVVM 145


 >gi|7441281|pir||T12632  water channel protein - common sunflower
            >gi|1212915|emb|CAA65186.1| (X95952) aquaporin [Helianthus annuus]
            Length = 248

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 308 (108.4 bits), Expect = 1.2e-38, Sum P(2) = 1.2e-38
 Identities = 57/102 (55%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q + +  A YLL +++GG A P H +A+GVG  QGVV EI++TF+L++ VYAT VDP
Sbjct:   105 WIAQCIGSIAACYLLSFVTGGLAVPTHAVAAGVGAIQGVVMEIIITFALVYNVYATAVDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG L  + P  +G +VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGDLGTIAPLAIGLIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 131 (46.1 bits), Expect = 1.2e-38, Sum P(2) = 1.2e-38
 Identities = 21/30 (70%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ AG+++ HW YWVGPL+GGGLAG IY
Sbjct:   203 GPAVAAGDFSGHWIYWVGPLVGGGLAGAIY 232

 Score = 38 (13.4 bits), Expect = 8.2e-10, Sum P(2) = 8.2e-10
 Identities = 24/90 (26%), Positives = 37/90 (41%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQ-----GVVWEI------VLTFSLLFTVYATMVDPKKGALA 209
             +SGG   P  T    VG GQ     G+ + I      +    LL  V   +  P     A
Sbjct:    77 ISGGHVNPAVTFGLAVG-GQITILTGIFYWIAQCIGSIAACYLLSFVTGGLAVPTHAVAA 135

Query:   210 GLGPTLVGFVVGANILAGGAYS--AASMNPAR 299
             G+G  + G V+   I     Y+  A +++P +
Sbjct:   136 GVG-AIQGVVMEIIITFALVYNVYATAVDPKK 166


 >gi|11276918|pir||T48884  membrane channel protein [imported] - potato
            (fragment) >gi|1518057|gb|AAB67881.1| (U65700) membrane channel
            protein [Solanum tuberosum]
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           __________
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 301 (106.0 bits), Expect = 1.2e-38, Sum P(2) = 1.2e-38
 Identities = 55/102 (53%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ +  A  LL Y++ G A P H +A+G+   +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WVAQLLGSTVACLLLKYVTNGLAVPTHGVAAGMNGAEGVVMEIVITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 138 (48.6 bits), Expect = 1.2e-38, Sum P(2) = 1.2e-38
 Identities = 26/44 (59%), Positives = 34/44 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY-ETF--XH*PISCS 429
             GPA+VAG+++ +W YWVGPLIGGGLAG+IY + F   H P+  S
Sbjct:   203 GPAVVAGDFSQNWIYWVGPLIGGGLAGFIYGDVFIGSHTPLPTS 246

 Score = 40 (14.1 bits), Expect = 7.6e-11, Sum P(2) = 7.6e-11
 Identities = 19/61 (31%), Positives = 24/61 (39%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYG----QGVV-WEIVL---TFSLLFTVYAT--MVDPKKGALAG 212
             +SGG   P  TL   VG       G+  W   L   T + L   Y T  +  P  G  AG
Sbjct:    77 ISGGHLNPAVTLGLAVGGNITTLTGLFYWVAQLLGSTVACLLLKYVTNGLAVPTHGVAAG 136

Query:   213 L 215
             +
Sbjct:   137 M 137

 Score = 37 (13.0 bits), Expect = 4.7e-28, Sum P(2) = 4.7e-28
 Identities = 16/44 (36%), Positives = 21/44 (47%), Frame = +2

Query:   317 WSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLPRDEE 448
             W    GP+IG  G GL+     +   +   FI  SH PLP  E+
Sbjct:   215 WIYWVGPLIG--G-GLA---GFIYGDV---FIG-SHTPLPTSED 248


 >gi|7441271|pir||T14001  aquaporin TIP18 - common sunflower
            >gi|1212923|emb|CAA65185.1| (X95951) aquaporin [Helianthus annuus]
            Length = 248

Frame  3 hits (HSPs):                  __________________________         
Frame  2 hits (HSPs):                                              _______
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 320 (112.6 bits), Expect = 1.5e-38, Sum P(2) = 1.5e-38
 Identities = 59/100 (59%), Positives = 77/100 (77%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL +++GG A P H +A+GVG  QGVV+EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSVAACFLLSFVTGGLAVPTHGVAAGVGVLQGVVFEIIITFALVYTVYATACDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGP 311
             KKGAL  + P  +GF+VGANILA G +S  SMNPARSFGP
Sbjct:   165 KKGALGTISPIAIGFIVGANILAAGPFSGGSMNPARSFGP 204

 Score = 118 (41.5 bits), Expect = 1.5e-38, Sum P(2) = 1.5e-38
 Identities = 20/30 (66%), Positives = 24/30 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GP + A +++ HW YWVGPLIGGGLAG IY
Sbjct:   203 GPTVAAFDFSGHWFYWVGPLIGGGLAGAIY 232

 Score = 41 (14.4 bits), Expect = 2.7e-06, Sum P(2) = 2.7e-06
 Identities = 23/68 (33%), Positives = 27/68 (39%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV------WEIVLTFS-----LLFTVYATMVDPKKGALA 209
             +SGG   P  T    VG GQ  +      W   L  S     LL  V   +  P  G  A
Sbjct:    77 ISGGHVNPAVTFGLAVG-GQITILTGLFYWIAQLLGSVAACFLLSFVTGGLAVPTHGVAA 135

Query:   210 GLGPTLVGFV 239
             G+G  L G V
Sbjct:   136 GVG-VLQGVV 144

 Score = 40 (14.1 bits), Expect = 2.3e-30, Sum P(2) = 2.3e-30
 Identities = 15/42 (35%), Positives = 19/42 (45%), Frame = +2

Query:   317 WSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLPRD 442
             W    GP+IG  G GL+  +   V      FI   H+PL  D
Sbjct:   215 WFYWVGPLIG--G-GLAGAIYPNV------FISNEHIPLTND 247


 >gi|2058706|gb|AAB53329.1|  (U95008) Rb7 [Lycopersicon esculentum]
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           __________
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 301 (106.0 bits), Expect = 1.5e-38, Sum P(2) = 1.5e-38
 Identities = 55/102 (53%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ +  A  LL Y++ G A P H +A+G+   +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WVAQLLGSTVACLLLKYVTNGLAVPTHGVAAGMSGAEGVVMEIVITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 137 (48.2 bits), Expect = 1.5e-38, Sum P(2) = 1.5e-38
 Identities = 26/44 (59%), Positives = 34/44 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY-ETFX--H*PISCS 429
             GPA+VAG+++ +W YWVGPLIGGGLAG+IY + F   H P+  S
Sbjct:   203 GPAVVAGDFSQNWIYWVGPLIGGGLAGFIYGDVFIGCHTPLPTS 246

 Score = 41 (14.4 bits), Expect = 8.1e-11, Sum P(2) = 8.1e-11
 Identities = 18/61 (29%), Positives = 24/61 (39%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----WEIVL---TFSLLFTVYAT--MVDPKKGALAG 212
             +SGG   P  TL   VG    ++     W   L   T + L   Y T  +  P  G  AG
Sbjct:    77 ISGGHLNPAVTLGLAVGGNITILTGLFYWVAQLLGSTVACLLLKYVTNGLAVPTHGVAAG 136

Query:   213 L 215
             +
Sbjct:   137 M 137


 >gi|7441273|pir||T14002  aquaporin TIP7 - common sunflower
            >gi|1212925|emb|CAA65184.1| (X95950) aquaporin [Helianthus annuus]
            Length = 248

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 317 (111.6 bits), Expect = 2.0e-38, Sum P(2) = 2.0e-38
 Identities = 58/102 (56%), Positives = 77/102 (75%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL +++GG A P H +A GVG  QGVV+EI++TF++++TVYAT  DP
Sbjct:   105 WIAQLLGSTVACFLLSFVTGGLAVPTHGVAEGVGTIQGVVFEIIITFAMVYTVYATACDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKGAL  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGALGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 120 (42.2 bits), Expect = 2.0e-38, Sum P(2) = 2.0e-38
 Identities = 19/30 (63%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ + +++ HW YWVGPL+GGGLAG IY
Sbjct:   203 GPAVASFDFSGHWIYWVGPLVGGGLAGLIY 232

 Score = 39 (13.7 bits), Expect = 1.6e-06, Sum P(2) = 1.6e-06
 Identities = 27/90 (30%), Positives = 36/90 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQ-----GVVWEI-------VLTFSLLFTVYATMVDPKKGAL 206
             + GG   P  TL   VG GQ     G+ + I       V  F L F      V P  G  
Sbjct:    77 ICGGHVNPAVTLGLAVG-GQITFLTGLFYWIAQLLGSTVACFLLSFVTGGLAV-PTHGVA 134

Query:   207 AGLGPTLVGFVVGANILAGGAYS--AASMNPAR 299
              G+G T+ G V    I     Y+  A + +P +
Sbjct:   135 EGVG-TIQGVVFEIIITFAMVYTVYATACDPKK 166


 >gi|267135|sp|P24422|TIP2_TOBAC  TONOPLAST INTRINSIC PROTEIN, ROOT-SPECIFIC
            RB7-18C (RT-TIP) >gi|82191|pir||JQ1012 TobRB7-18C protein - common
            tobacco
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           __________
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 301 (106.0 bits), Expect = 4.1e-38, Sum P(2) = 4.1e-38
 Identities = 56/102 (54%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL Y++ G A P H +A+G+   QGVV EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSTVACLLLKYVTNGLAVPTHGVAAGLNGFQGVVMEIIITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 133 (46.8 bits), Expect = 4.1e-38, Sum P(2) = 4.1e-38
 Identities = 25/44 (56%), Positives = 33/44 (75%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY-ETFX--H*PISCS 429
             GPA+VAG+++ +W YW GPLIGGGLAG+IY + F   H P+  S
Sbjct:   203 GPAVVAGDFSQNWIYWAGPLIGGGLAGFIYGDVFIGCHTPLPTS 246

 Score = 42 (14.8 bits), Expect = 1.9e-10, Sum P(2) = 1.9e-10
 Identities = 19/61 (31%), Positives = 24/61 (39%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----WEIVL---TFSLLFTVYAT--MVDPKKGALAG 212
             +SGG   P  TL   VG    ++     W   L   T + L   Y T  +  P  G  AG
Sbjct:    77 ISGGHLNPAVTLGLAVGGNITILTGFFYWIAQLLGSTVACLLLKYVTNGLAVPTHGVAAG 136

Query:   213 L 215
             L
Sbjct:   137 L 137


 >gi|8699606|gb|AAF78758.1|AF271661_1  (AF271661) putative aquaporin TIP1 [Vitis
            berlandieri x Vitis rupestris]
            Length = 249

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 249
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 309 (108.8 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 59/102 (57%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QLV +  A +LL  ++GG  TPVH+L +GVG    +V+EIV+TF+L++TVYAT VDP
Sbjct:   105 WIAQLVGSILACFLLKLVTGGLTTPVHSLGAGVGVIDAIVFEIVITFALVYTVYATAVDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +G VVGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGIIAPIAIGLVVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 124 (43.7 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 19/30 (63%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G++ D+W YWVGPLIGGG+ G +Y
Sbjct:   203 GPAVVSGDFKDNWIYWVGPLIGGGMGGSVY 232


 >gi|10047074|emb|CAB95746.2|  (AJ289866) putative aquaporin [Vitis vinifera]
            Length = 249

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 249
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 309 (108.8 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 59/102 (57%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QLV +  A +LL  ++GG  TPVH+L +GVG    +V+EIV+TF+L++TVYAT VDP
Sbjct:   105 WIAQLVGSILACFLLKLVTGGLTTPVHSLGAGVGVIDAIVFEIVITFALVYTVYATAVDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +G VVGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGIIAPIAIGLVVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 124 (43.7 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 19/30 (63%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G++ D+W YWVGPLIGGG+ G +Y
Sbjct:   203 GPAVVSGDFKDNWIYWVGPLIGGGMGGSVY 232


 >gi|7441272|pir||T14000  aquaporin TIP7 - common sunflower
            >gi|1212921|emb|CAA65187.1| (X95953) aquaporin [Helianthus annuus]
            Length = 250

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 305 (107.4 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 56/102 (54%), Positives = 75/102 (73%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q + +  A +LL +++GG A P H +A G+   QGVV EI++TF+L++TVYAT VDP
Sbjct:   105 WIAQCLGSIVACFLLQFVTGGLAVPTHGVADGMNGVQGVVMEIIITFALVYTVYATAVDP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPMAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 128 (45.1 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 20/30 (66%), Positives = 28/30 (93%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G+++ +W YWVGPLIGGGLAG+I+
Sbjct:   203 GPAVVSGDFSQNWIYWVGPLIGGGLAGFIF 232


 >gi|126959|sp|P21653|TIP1_TOBAC  TONOPLAST INTRINSIC PROTEIN, ROOT-SPECIFIC
            RB7-5A (RT-TIP) >gi|82192|pir||JQ1011 TobRB7-5A protein - common
            tobacco >gi|100371|pir||S13719 probable membrane channel protein
            RB7 - common tobacco >gi|20011|emb|CAA38634.1| (X54855) possible
            membrane channel protein [Nicotiana tabacum]
            >gi|7580481|gb|AAB23597.2| (S45406) root-specific gene regulator
            [Nicotiana tabacum]
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           __________
Annotated Domains:        _____________________________________________   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200
__________________

Annotated Domains:
   BLOCKS               BL00221A: MIP family proteins.           23..33
   BLOCKS               BL00221B: MIP family proteins.           77..87
   BLOCKS               BL00221C: MIP family proteins.           146..162
   BLOCKS               BL00221D: MIP family proteins.           192..206
   BLOCKS               BL00221E: MIP family proteins.           214..224
   PFAM                 MIP: Major intrinsic protein             11..232
   PRINTS               MINTRINSICP1: MIP family motif I - 3     19..38
   PRINTS               MINTRINSICP2: MIP family motif II - 3    63..87
   PRINTS               MINTRINSICP3: MIP family motif III - 3   100..119
   PRINTS               MINTRINSICP4: MIP family motif IV - 3    145..163
   PRINTS               MINTRINSICP5: MIP family motif V - 3     178..200
   PRINTS               MINTRINSICP6: MIP family motif VI - 3    215..235
   PRODOM               PD000295: GLPF(9) AQP1(5) AQP2(4)        14..231
   PROSITE              MIP: MIP family signature.               81..89
__________________


  Plus Strand HSPs:

 Score = 300 (105.6 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 56/102 (54%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL Y++ G A P H +A+G+   QGVV EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSTVACLLLKYVTNGLAVPTHGVAAGLNGLQGVVMEIIITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 133 (46.8 bits), Expect = 5.2e-38, Sum P(2) = 5.2e-38
 Identities = 25/44 (56%), Positives = 33/44 (75%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY-ETFX--H*PISCS 429
             GPA+VAG+++ +W YW GPLIGGGLAG+IY + F   H P+  S
Sbjct:   203 GPAVVAGDFSQNWIYWAGPLIGGGLAGFIYGDVFIGCHTPLPTS 246

 Score = 45 (15.8 bits), Expect = 9.3e-11, Sum P(2) = 9.3e-11
 Identities = 28/94 (29%), Positives = 38/94 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----WEIVL---TFSLLFTVYAT--MVDPKKGALAG 212
             +SGG   P  TL   VG    ++     W   L   T + L   Y T  +  P  G  AG
Sbjct:    77 ISGGHLNPAVTLGLAVGGNITILTGFFYWIAQLLGSTVACLLLKYVTNGLAVPTHGVAAG 136

Query:   213 LGPTLVGFVVGANILAGGAYS--AASMNPAR-SFG 308
             L   L G V+   I     Y+  A + +P + S G
Sbjct:   137 LNG-LQGVVMEIIITFALVYTVYATAADPKKGSLG 170


 >gi|7441255|pir||T01648  probable tonoplast aquaporin - maize
            >gi|3264596|gb|AAC24569.1| (AF057183) putative tonoplast aquaporin
            [Zea mays]
            Length = 248

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 303 (106.7 bits), Expect = 6.6e-38, Sum P(2) = 6.6e-38
 Identities = 57/102 (55%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ A+ A +LL Y++ GQA P H + SG+   +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WVAQLLGASVACFLLQYVTHGQAIPTHGV-SGISEIEGVVMEIVITFALVYTVYATAADP 163

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   164 KKGSLGTIAPMAIGFIVGANILAAGPFSGGSMNPARSFGPAV 205

 Score = 129 (45.4 bits), Expect = 6.6e-38, Sum P(2) = 6.6e-38
 Identities = 20/30 (66%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ AGN+  +W YWVGPL+GGGLAG +Y
Sbjct:   202 GPAVAAGNFAGNWVYWVGPLVGGGLAGLVY 231


 >gi|9623335|gb|AAF90121.1|AF254799_1  (AF254799) tonoplast intrinsic protein 1
            [Hordeum vulgare]
            Length = 249

Frame  3 hits (HSPs):                       _____________________         
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 249
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 292 (102.8 bits), Expect = 1.1e-37, Sum P(2) = 1.1e-37
 Identities = 55/102 (53%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ A+ A  LL +++  QA P H + SG+   +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WVAQLLGASVACLLLQFVTHAQAMPTHAV-SGISEVEGVVMEIVITFALVYTVYATAADP 163

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   164 KKGSLGTIAPMAIGFIVGANILAAGPFSGGSMNPARSFGPAV 205

 Score = 138 (48.6 bits), Expect = 1.1e-37, Sum P(2) = 1.1e-37
 Identities = 22/30 (73%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ AGN++ HW YWVGPLIGGGLAG +Y
Sbjct:   202 GPAVAAGNFSGHWVYWVGPLIGGGLAGLVY 231


 >gi|4099406|gb|AAD10494.1|  (U86762) gamma-type tonoplast intrinsic protein
            [Triticum aestivum]
            Length = 250

Frame  3 hits (HSPs):                  ____________________________       
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 297 (104.5 bits), Expect = 1.7e-37, Sum P(2) = 1.7e-37
 Identities = 59/106 (55%), Positives = 75/106 (70%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL + +GG  T    L SG+G  + VV EIV+TF L++TVYAT VDP
Sbjct:   107 WIAQLLGSTAACFLLRFSTGGLPTGTFGL-SGIGAWEAVVLEIVMTFGLVYTVYATAVDP 165

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWK 329
             KKG+L  + P  +GF+VGANIL GGA+S ASMNPA SFGP L  W+
Sbjct:   166 KKGSLGTIAPIAIGFIVGANILVGGAFSGASMNPAVSFGPALVSWE 211

 Score = 131 (46.1 bits), Expect = 1.7e-37, Sum P(2) = 1.7e-37
 Identities = 23/31 (74%), Positives = 24/31 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+  W   W YWVGPLIGGGLAG IYE
Sbjct:   204 GPALVSWEWGYQWVYWVGPLIGGGLAGVIYE 234

 Score = 53 (18.7 bits), Expect = 2.6e-29, Sum P(2) = 2.6e-29
 Identities = 17/44 (38%), Positives = 21/44 (47%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLP 436
             W   W    GP+IG  G GL+ V+        L FI R+H  LP
Sbjct:   212 WGYQWVYWVGPLIG--G-GLAGVI------YELLFISRTHEQLP 246

 Score = 38 (13.4 bits), Expect = 8.7e-10, Sum P(2) = 8.7e-10
 Identities = 23/82 (28%), Positives = 34/82 (41%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVV 242
             +SGG   P  T  + VG G      I L   LL+ + A ++         L  +  G   
Sbjct:    79 ISGGHVNPAVTFGAFVG-GN-----ITLFRGLLYWI-AQLLGSTAACFL-LRFSTGGLPT 130

Query:   243 GANILAG-GAYSAASMNPARSFG 308
             G   L+G GA+ A  +    +FG
Sbjct:   131 GTFGLSGIGAWEAVVLEIVMTFG 153


 >gi|3004950|gb|AAC09245.1|  (AF037061) tonoplast intrinsic protein; ZmTIP1 [Zea
            mays]
            Length = 250

Frame  3 hits (HSPs):              ________  ______________________       
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 294 (103.5 bits), Expect = 3.6e-37, Sum P(2) = 3.6e-37
 Identities = 57/106 (53%), Positives = 75/106 (70%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ +  A +LL + +GGQAT    L +GV   + +V EIV+TF L++TVYAT VDP
Sbjct:   107 WVAQLLGSTVACFLLRFSTGGQATGTFGL-TGVSVWEALVLEIVMTFGLVYTVYATAVDP 165

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWK 329
             KKG+L  + P  +GF+VGANIL GGA+  ASMNPA SFGP L  W+
Sbjct:   166 KKGSLGTIAPIAIGFIVGANILVGGAFDGASMNPAVSFGPALVSWE 211

 Score = 131 (46.1 bits), Expect = 3.6e-37, Sum P(2) = 3.6e-37
 Identities = 23/31 (74%), Positives = 24/31 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+  W   W YWVGPLIGGGLAG IYE
Sbjct:   204 GPALVSWEWGYQWVYWVGPLIGGGLAGVIYE 234

 Score = 48 (16.9 bits), Expect = 1.8e-28, Sum P(2) = 1.8e-28
 Identities = 16/44 (36%), Positives = 20/44 (45%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLP 436
             W   W    GP+IG  G GL+ V+        L FI  +H  LP
Sbjct:   212 WGYQWVYWVGPLIG--G-GLAGVI------YELLFISHTHEQLP 246

 Score = 37 (13.0 bits), Expect = 1.1e-09, Sum P(2) = 1.1e-09
 Identities = 13/38 (34%), Positives = 19/38 (50%), Frame = +3

Query:    24 LVAAATA-SYLLYY-------LSGGQATPVHTLASGVG 113
             L+AAA A ++ L+        +SGG   P  T  + VG
Sbjct:    58 LIAAAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVG 95


 >gi|7441268|pir||T14314  probable membrane protein - carrot
            >gi|1794147|dbj|BAA19129.1| (AB000506) similar to EMBL Accession
            Number : X54855 [Daucus carota]
            Length = 248

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 304 (107.0 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 54/102 (52%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q + +  A +LL +++ G+A P H + +G+G  +GVV+EIV+TF+L++TVYAT  DP
Sbjct:   105 WIAQCLGSTVACFLLKFVTAGKAIPTHGVGAGLGAAEGVVFEIVITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 120 (42.2 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 19/30 (63%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ + +++ HW YWVGPLIGGGLAG +Y
Sbjct:   203 GPAVASFDFSGHWIYWVGPLIGGGLAGLVY 232

 Score = 41 (14.4 bits), Expect = 1.0e-06, Sum P(2) = 1.0e-06
 Identities = 20/62 (32%), Positives = 24/62 (38%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----W------EIVLTFSLLFTVYATMVDPKKGALA 209
             +SGG   P  TL   +G    ++     W        V  F L F V A    P  G  A
Sbjct:    77 ISGGHLNPAVTLGLAIGGNITIITGLFYWIAQCLGSTVACFLLKF-VTAGKAIPTHGVGA 135

Query:   210 GLG 218
             GLG
Sbjct:   136 GLG 138


 >gi|629784|pir||S47037  tonoplast intrinsic protein gamma - barley
            >gi|520936|emb|CAA56553.1| (X80266) gamma-TIP-like protein [Hordeum
            vulgare]
            Length = 250

Frame  3 hits (HSPs):                  ____________________________       
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           _______   
Annotated Domains:                      ___                               
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200
__________________

Annotated Domains:
   PROSITE              MIP: MIP family signature.               83..91
__________________


  Plus Strand HSPs:

 Score = 293 (103.1 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 57/106 (53%), Positives = 75/106 (70%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ +  A +LL + +GG  T    L +G+G  + VV EIV+TF L++TVYAT VDP
Sbjct:   107 WVAQLLGSTAACFLLRFSTGGLPTGTFGL-TGIGAWEAVVLEIVMTFGLVYTVYATAVDP 165

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWK 329
             KKG+L  + P  +GF+VGANIL GGA+S ASMNPA SFGP L  W+
Sbjct:   166 KKGSLGTIAPIAIGFIVGANILVGGAFSGASMNPAVSFGPALVSWE 211

 Score = 131 (46.1 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 23/31 (74%), Positives = 24/31 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+  W   W YWVGPLIGGGLAG IYE
Sbjct:   204 GPALVSWEWGYQWVYWVGPLIGGGLAGVIYE 234

 Score = 53 (18.7 bits), Expect = 6.9e-29, Sum P(2) = 6.9e-29
 Identities = 17/44 (38%), Positives = 21/44 (47%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLP 436
             W   W    GP+IG  G GL+ V+        L FI R+H  LP
Sbjct:   212 WGYQWVYWVGPLIG--G-GLAGVI------YELLFISRTHEQLP 246

 Score = 38 (13.4 bits), Expect = 8.7e-10, Sum P(2) = 8.7e-10
 Identities = 24/82 (29%), Positives = 33/82 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGFVV 242
             +SGG   P  T  + VG G      I L   LL+ V A ++         L  +  G   
Sbjct:    79 ISGGHVNPAVTFGAFVG-GN-----ITLFRGLLYWV-AQLLGSTAACFL-LRFSTGGLPT 130

Query:   243 GANILAG-GAYSAASMNPARSFG 308
             G   L G GA+ A  +    +FG
Sbjct:   131 GTFGLTGIGAWEAVVLEIVMTFG 153


 >gi|11276920|pir||T48886  aquaporin [imported] - Vernicia fordii
            >gi|3249565|gb|AAC39480.1| (AF047173) aquaporin [Vernicia fordii]
            Length = 248

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 293 (103.1 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 55/102 (53%), Positives = 74/102 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL  ++GG A P H++A  VG  +GVV EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSIVACFLLKLVTGGLAIPTHSVAGRVGAIEGVVMEIIITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SM PARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMYPARSFGPAV 206

 Score = 131 (46.1 bits), Expect = 4.6e-37, Sum P(2) = 4.6e-37
 Identities = 22/30 (73%), Positives = 27/30 (90%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G++ D+W YWVGPLIGGGLAG IY
Sbjct:   203 GPAVVSGDFHDNWIYWVGPLIGGGLAGLIY 232


 >gi|7441289|pir||T10804  tonoplast intrinsic protein, delta type - upland cotton
            >gi|1439609|gb|AAB04557.1| (U62778) delta-tonoplast intrinsic
            protein [Gossypium hirsutum]
            Length = 248

Frame  3 hits (HSPs):                       ______________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 308 (108.4 bits), Expect = 5.8e-37, Sum P(2) = 5.8e-37
 Identities = 57/102 (55%), Positives = 75/102 (73%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL  ++GG   P+H L +GVG  QGVV EI++TF+L++TVYAT  DP
Sbjct:   105 WIAQLLGSIVACFLLKAVTGGLTVPIHGLGAGVGAIQGVVMEIIITFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 115 (40.5 bits), Expect = 5.8e-37, Sum P(2) = 5.8e-37
 Identities = 19/30 (63%), Positives = 24/30 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ +G++   W YWVGPLIGGGL+G IY
Sbjct:   203 GPAVASGDFNGIWIYWVGPLIGGGLSGLIY 232


 >gi|12006843|gb|AAG44946.1|AF290619_1  (AF290619) putative gamma TIP [Nicotiana
            glauca]
            Length = 251

Frame  3 hits (HSPs):                ______  _____________________        
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 287 (101.0 bits), Expect = 1.5e-36, Sum P(2) = 1.5e-36
 Identities = 55/104 (52%), Positives = 73/104 (70%), Frame = +3

Query:    15 IDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPK 194
             + QL+ +  A +LL + +GG +T    L++GV      V+EIV+TF L++TVYAT VDPK
Sbjct:   108 VAQLLGSTVACFLLEFATGGMSTGAFALSAGVSVWNAFVFEIVMTFGLVYTVYATAVDPK 167

Query:   195 KGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRW 326
             KG L  + P  +GF+VGANILAGGA++ ASMNPA SFGP L  W
Sbjct:   168 KGDLGVIAPIAIGFIVGANILAGGAFTGASMNPAVSFGPALVSW 211

 Score = 132 (46.5 bits), Expect = 1.5e-36, Sum P(2) = 1.5e-36
 Identities = 20/31 (64%), Positives = 24/31 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+  WT  W YW GPL+GGG+AG +YE
Sbjct:   205 GPALVSWTWTHQWVYWAGPLVGGGIAGVVYE 235

 Score = 58 (20.4 bits), Expect = 8.7e-29, Sum P(2) = 8.7e-29
 Identities = 18/44 (40%), Positives = 23/44 (52%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLP 436
             W   W    GP++G  G G++ VV  LV      FI+ SH PLP
Sbjct:   213 WTHQWVYWAGPLVG--G-GIAGVVYELV------FINHSHEPLP 247

 Score = 43 (15.1 bits), Expect = 2.1e-10, Sum P(2) = 2.1e-10
 Identities = 14/31 (45%), Positives = 18/31 (58%), Frame = +3

Query:   228 VGFVVGANILAGGAYSAASMNPARSFGPCLG 320
             V   VGANI +GG      +NPA +FG  +G
Sbjct:    71 VAVSVGANI-SGG-----HVNPAVTFGAFVG 95


 >gi|4138262|emb|CAA69353.1|  (Y08161) aquaporin 1 [Nicotiana tabacum]
            Length = 251

Frame  3 hits (HSPs):                ______  _____________________        
Frame  2 hits (HSPs):                                             ________
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 287 (101.0 bits), Expect = 1.5e-36, Sum P(2) = 1.5e-36
 Identities = 55/104 (52%), Positives = 73/104 (70%), Frame = +3

Query:    15 IDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPK 194
             + QL+ +  A +LL + +GG +T    L++GV      V+EIV+TF L++TVYAT VDPK
Sbjct:   108 VAQLLGSTVACFLLEFATGGMSTGAFALSAGVSVWNAFVFEIVMTFGLVYTVYATAVDPK 167

Query:   195 KGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRW 326
             KG L  + P  +GF+VGANILAGGA++ ASMNPA SFGP L  W
Sbjct:   168 KGDLGVIAPIAIGFIVGANILAGGAFTGASMNPAVSFGPALVSW 211

 Score = 132 (46.5 bits), Expect = 1.5e-36, Sum P(2) = 1.5e-36
 Identities = 20/31 (64%), Positives = 24/31 (77%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+  WT  W YW GPL+GGG+AG +YE
Sbjct:   205 GPALVSWTWTHQWVYWAGPLVGGGIAGVVYE 235

 Score = 57 (20.1 bits), Expect = 1.1e-28, Sum P(2) = 1.1e-28
 Identities = 17/44 (38%), Positives = 23/44 (52%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLP 436
             W   W    GP++G  G G++ VV  L+      FI+ SH PLP
Sbjct:   213 WTHQWVYWAGPLVG--G-GIAGVVYELI------FINHSHEPLP 247

 Score = 43 (15.1 bits), Expect = 2.1e-10, Sum P(2) = 2.1e-10
 Identities = 14/31 (45%), Positives = 18/31 (58%), Frame = +3

Query:   228 VGFVVGANILAGGAYSAASMNPARSFGPCLG 320
             V   VGANI +GG      +NPA +FG  +G
Sbjct:    71 VAVSVGANI-SGG-----HVNPAVTFGAFVG 95


 >gi|12006841|gb|AAG44945.1|AF290618_1  (AF290618) putative delta TIP [Nicotiana
            glauca]
            Length = 248

Frame  3 hits (HSPs):     _________________  _____________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 295 (103.8 bits), Expect = 5.1e-36, Sum P(2) = 5.1e-36
 Identities = 56/101 (55%), Positives = 74/101 (73%), Frame = +3

Query:    15 IDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPK 194
             I QL+ +  A  LL  ++GG A P H +A+GVG  +GVV EI++TF+L++TVYAT  DPK
Sbjct:   106 IAQLLGSIVACLLLKVVTGGLAVPTHNVAAGVGALEGVVMEIIITFALVYTVYATAADPK 165

Query:   195 KGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   166 KGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 119 (41.9 bits), Expect = 5.1e-36, Sum P(2) = 5.1e-36
 Identities = 18/30 (60%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ +G++T++W YW GPL+GGGLAG  Y
Sbjct:   203 GPAVASGDFTNNWIYWAGPLVGGGLAGLTY 232

 Score = 54 (19.0 bits), Expect = 7.0e-08, Sum P(2) = 7.0e-08
 Identities = 24/79 (30%), Positives = 34/79 (43%), Frame = +3

Query:   108 VGYGQGVVWEIVLTFSLLFT-VYATMVDPKKGALAGLGPT-LV------GFVVGANILAG 263
             VG  +  + E + T   +F  V + +   K  A A L P  LV      GF +   +  G
Sbjct:    15 VGSLKAYLAEFISTLLFVFAGVGSAIAYNKLTANAALDPAGLVAVAVCHGFALFVAVAVG 74

Query:   264 GAYSAASMNPARSFGPCLG 320
                S   +NPA +FG  LG
Sbjct:    75 ANISGGHVNPAVTFGLALG 93


 >gi|4884870|gb|AAD31849.1|AF133533_1  (AF133533) water channel protein MipL
            [Mesembryanthemum crystallinum]
            Length = 206

Frame  3 hits (HSPs):                 ____________________________        
Frame  1 hits (HSPs):                                         ________    
                        __________________________________________________
Database sequence:     |           |            |           |           | | 206
                       0          50          100         150         200

  Plus Strand HSPs:

 Score = 291 (102.4 bits), Expect = 5.1e-36, Sum P(2) = 5.1e-36
 Identities = 53/110 (48%), Positives = 76/110 (69%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ Q++ +  A  LL +++ G + P H +ASG+   +G+V EIV+TF+L++TVYAT  DP
Sbjct:    61 WVAQVLGSIVACLLLKFVTNGMSIPAHGVASGMNAFEGIVMEIVITFALVYTVYATAADP 120

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRS 341
             KKG +  + P  +GF+VGANILA G +S  SMNPARSFGP +    L +S
Sbjct:   121 KKGTIGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAIVSGDLSQS 170

 Score = 123 (43.3 bits), Expect = 5.1e-36, Sum P(2) = 5.1e-36
 Identities = 20/30 (66%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G+ +  W YWVGPLIGGGLAG +Y
Sbjct:   159 GPAIVSGDLSQSWIYWVGPLIGGGLAGLVY 188


 >gi|7441256|pir||F71442  probable membrane channel protein - Arabidopsis
            thaliana >gi|2245093|emb|CAB10515.1| (Z97343) membrane channel like
            protein [Arabidopsis thaliana] >gi|7268486|emb|CAB78737.1|
            (AL161546) membrane channel like protein [Arabidopsis thaliana]
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 296 (104.2 bits), Expect = 6.5e-36, Sum P(2) = 6.5e-36
 Identities = 54/102 (52%), Positives = 75/102 (73%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q + +  A  LL +++ G++ P H +A+G+G  +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WIAQCLGSIVACLLLVFVTNGESVPTHGVAAGLGAIEGVVMEIVVTFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 117 (41.2 bits), Expect = 6.5e-36, Sum P(2) = 6.5e-36
 Identities = 19/30 (63%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G+++  W YWVGPL+GG LAG IY
Sbjct:   203 GPAVVSGDFSQIWIYWVGPLVGGALAGLIY 232

 Score = 50 (17.6 bits), Expect = 4.8e-07, Sum P(2) = 4.8e-07
 Identities = 28/94 (29%), Positives = 38/94 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----W------EIVLTFSLLFTVYATMVDPKKGALA 209
             +SGG   P  TL   VG    V+     W       IV    L+F      V P  G  A
Sbjct:    77 ISGGHLNPAVTLGLAVGGNITVITGFFYWIAQCLGSIVACLLLVFVTNGESV-PTHGVAA 135

Query:   210 GLGPTLVGFVVGANILAGGAYS--AASMNPAR-SFG 308
             GLG  + G V+   +     Y+  A + +P + S G
Sbjct:   136 GLG-AIEGVVMEIVVTFALVYTVYATAADPKKGSLG 170


 >gi|9758773|dbj|BAB09071.1|  (AB025628) membrane channel protein-like; aquaporin
            (tonoplast intrinsic protein)-like [Arabidopsis thaliana]
            Length = 250

Frame  3 hits (HSPs):                  ___________________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200

  Plus Strand HSPs:

 Score = 293 (103.1 bits), Expect = 2.8e-35, Sum P(2) = 2.8e-35
 Identities = 53/102 (51%), Positives = 75/102 (73%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q + +  A  LL +++ G++ P H +++G+G  +GVV EIV+TF+L++TVYAT  DP
Sbjct:   105 WIAQCLGSIVACLLLVFVTNGKSVPTHGVSAGLGAVEGVVMEIVVTFALVYTVYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPARSFGP +
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAV 206

 Score = 114 (40.1 bits), Expect = 2.8e-35, Sum P(2) = 2.8e-35
 Identities = 19/30 (63%), Positives = 24/30 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+V+G+ +  W YWVGPL+GG LAG IY
Sbjct:   203 GPAVVSGDLSQIWIYWVGPLVGGALAGLIY 232

 Score = 44 (15.5 bits), Expect = 6.8e-06, Sum P(2) = 6.8e-06
 Identities = 26/94 (27%), Positives = 38/94 (40%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVV-----W------EIVLTFSLLFTVYATMVDPKKGALA 209
             +SGG   P  TL   +G    ++     W       IV    L+F      V P  G  A
Sbjct:    77 ISGGHLNPAVTLGLAIGGNITLITGFFYWIAQCLGSIVACLLLVFVTNGKSV-PTHGVSA 135

Query:   210 GLGPTLVGFVVGANILAGGAYS--AASMNPAR-SFG 308
             GLG  + G V+   +     Y+  A + +P + S G
Sbjct:   136 GLG-AVEGVVMEIVVTFALVYTVYATAADPKKGSLG 170


 >gi|4099408|gb|AAD10495.1|  (U86763) delta-type tonoplast intrinsic protein
            [Triticum aestivum]
            Length = 248

Frame  3 hits (HSPs):                        _____________________        
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |          |         |         | 248
                       0        50       100        150       200

  Plus Strand HSPs:

 Score = 286 (100.7 bits), Expect = 2.8e-35, Sum P(2) = 2.8e-35
 Identities = 55/102 (53%), Positives = 73/102 (71%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             W+ QL+ A   ++L+ + +G  ATP H L SGVG  +GVV EI++TF L++TVYAT  DP
Sbjct:   106 WVAQLLGAIVGAFLVQFCTG-VATPTHGL-SGVGAFEGVVMEIIVTFGLVYTVYATAADP 163

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANIL  G +S  SMNPARSFGP +
Sbjct:   164 KKGSLGTIAPIAIGFIVGANILVAGPFSGGSMNPARSFGPAV 205

 Score = 121 (42.6 bits), Expect = 2.8e-35, Sum P(2) = 2.8e-35
 Identities = 19/30 (63%), Positives = 25/30 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ +G++T+ W YW GPLIGGGLAG +Y
Sbjct:   202 GPAVASGDFTNIWVYWAGPLIGGGLAGVVY 231


 >gi|4584429|emb|CAB40742.1|  (AJ237751) aquaglyceroporin [Nicotiana tabacum]
            Length = 247

Frame  3 hits (HSPs):      ________________ ________________________      
Frame  2 hits (HSPs):                                            _________
Frame  1 hits (HSPs):                                   _______________   
                        __________________________________________________
Database sequence:     |         |          |         |         |         | 247
                       0        50        100       150       200

  Plus Strand HSPs:

 Score = 382 (134.5 bits), Expect = 2.5e-34, P = 2.5e-34
 Identities = 75/122 (61%), Positives = 89/122 (72%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WIDQL+A+  A  LL YL+ G  TPVHTLA+GV YGQG++ E++LTFSLLFTVY T+VDP
Sbjct:   102 WIDQLLASVAACALLNYLTAGLETPVHTLANGVSYGQGIIMEVILTFSLLFTVYTTIVDP 161

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             KKG L G+GP L G VVGANI+AGG +S ASMNPARSFGP           G+  W  HW
Sbjct:   162 KKGILEGMGPLLTGLVVGANIMAGGPFSGASMNPARSFGPAFVS-------GI--WTDHW 212

Query:   372 -WW 377
              +W
Sbjct:   213 VYW 215

 Score = 160 (56.3 bits), Expect = 8.3e-11, P = 8.3e-11
 Identities = 34/72 (47%), Positives = 40/72 (55%), Frame = +1

Query:   190 PRREHLLGLGQRWLGLWLGPTSSQVGLTLLLL*TQQGLLGPALVAGNWTDHWAYWVGPLI 369
             P++  L G+G    GL +G      G             GPA V+G WTDHW YWVGPLI
Sbjct:   161 PKKGILEGMGPLLTGLVVGANIMAGGPFSGASMNPARSFGPAFVSGIWTDHWVYWVGPLI 220

Query:   370 GGGLAGYIYETF 405
             GGGLAG+I E F
Sbjct:   221 GGGLAGFICENF 232

 Score = 60 (21.1 bits), Expect = 2.7, Sum P(2) = 0.93
 Identities = 19/47 (40%), Positives = 24/47 (51%), Frame = +2

Query:   305 WALPWSLETGPIIGPTGLGLSLVVALLVTSMRLSFIDRSHVPLPRDE 445
             W   W    GP+IG  G GL+     +  +    FI R+HVPLP DE
Sbjct:   208 WTDHWVYWVGPLIG--G-GLA---GFICENF---FIVRTHVPLPSDE 245

 Score = 60 (21.1 bits), Expect = 2.7, Sum P(2) = 0.93
 Identities = 22/72 (30%), Positives = 31/72 (43%), Frame = +3

Query:   120 QGVVWEIVLTFSLLFT-VYATMVDPKKGA--LAGLGPTLVG--FVVGANILAGGAYSAAS 284
             Q ++ E + TF  +F  V + M   K     L  L    +    VV   I AG   S   
Sbjct:    19 QALIVEFICTFLFVFAGVGSAMAANKLNGDPLVSLFFVAMAHALVVAVTISAGFRISGGH 78

Query:   285 MNPARSFGPCLG 320
             +NPA + G C+G
Sbjct:    79 LNPAVTLGLCMG 90


 >gi|2246621|gb|AAB62692.1|  (AF004393) salt-stress induced tonoplast intrinsic
            protein [Arabidopsis thaliana]
            Length = 273

Frame  3 hits (HSPs):                          _____________________      
Frame  1 hits (HSPs):                                            ______   
                        __________________________________________________
Database sequence:     |        |         |        |        |        |    | 273
                       0       50       100      150      200      250

  Plus Strand HSPs:

 Score = 310 (109.1 bits), Expect = 1.1e-26, P = 1.1e-26
 Identities = 60/122 (49%), Positives = 82/122 (67%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL + +GG+  P   L++GVG    +V+EIV+TF L++TVYAT VDP
Sbjct:   128 WIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDP 187

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   188 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 238

Query:   372 -WW 377
              +W
Sbjct:   239 VYW 241

 Score = 140 (49.3 bits), Expect = 2.7e-08, P = 2.7e-08
 Identities = 22/31 (70%), Positives = 26/31 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+  WT+HW YW GPLIGGGLAG IY+
Sbjct:   226 GPAVVSWTWTNHWVYWAGPLIGGGLAGIIYD 256


 >gi|9293929|dbj|BAB01832.1|  (AB028611) salt-stress induced tonoplast intrinsic
            protein [Arabidopsis thaliana]
            Length = 253

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 310 (109.1 bits), Expect = 1.1e-26, P = 1.1e-26
 Identities = 60/122 (49%), Positives = 82/122 (67%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL + +GG+  P   L++GVG    +V+EIV+TF L++TVYAT VDP
Sbjct:   108 WIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYTVYATAVDP 167

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   168 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 218

Query:   372 -WW 377
              +W
Sbjct:   219 VYW 221

 Score = 140 (49.3 bits), Expect = 2.1e-08, P = 2.1e-08
 Identities = 22/31 (70%), Positives = 26/31 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+  WT+HW YW GPLIGGGLAG IY+
Sbjct:   206 GPAVVSWTWTNHWVYWAGPLIGGGLAGIIYD 236


 >gi|9988412|dbj|BAB12722.1|  (AB048248) gamma tonoplast intrinsic protein [Pyrus
            communis]
            Length = 252

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 252
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 310 (109.1 bits), Expect = 1.1e-26, P = 1.1e-26
 Identities = 61/122 (50%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ A  A  LL +++ GQ T   +L+SGVG     V+EIV+TF L++TVYAT +DP
Sbjct:   107 WIAQLLGATVACGLLKFVTNGQTTAAFSLSSGVGVWNAFVFEIVMTFGLVYTVYATAIDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G++  + P  +GF+VGANILAGGA+  ASMNPA SFGP L  W          W +HW
Sbjct:   167 KNGSVGTIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPALVSWS---------WENHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 135 (47.5 bits), Expect = 7.6e-08, P = 7.6e-08
 Identities = 22/31 (70%), Positives = 25/31 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+ +W +HW YW GPLIG GLAG IYE
Sbjct:   205 GPALVSWSWENHWVYWAGPLIGAGLAGLIYE 235


 >gi|1928981|gb|AAB51393.1|  (U92651) tonoplast intrinsic protein bobTIP26-1
            [Brassica oleracea var. botrytis]
            Length = 251

Frame  3 hits (HSPs):                  _____________________________      
Frame  1 hits (HSPs):                                           ________  
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 308 (108.4 bits), Expect = 1.7e-26, P = 1.7e-26
 Identities = 60/122 (49%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG   P   L++GVG    +V+EIV+TF L++TVYAT VDP
Sbjct:   107 WIAQLLGSVVACLLLKFTTGGLVVPAFGLSAGVGVSNALVFEIVMTFGLVYTVYATAVDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   167 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWS---------WNNHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 144 (50.7 bits), Expect = 8.9e-12, Sum P(2) = 8.9e-12
 Identities = 22/33 (66%), Positives = 27/33 (81%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+ +W +HW YW GPL+GGGLAG IYE F
Sbjct:   205 GPAVVSWSWNNHWVYWAGPLVGGGLAGLIYEVF 237

 Score = 42 (14.8 bits), Expect = 8.9e-12, Sum P(2) = 8.9e-12
 Identities = 19/62 (30%), Positives = 27/62 (43%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYG----QGVV-W------EIVLTFSLLFTVYATMVDPKKGALA 209
             +SGG   P  T  + VG      +G++ W       +V    L FT    +V P  G  A
Sbjct:    79 ISGGHVNPAVTFGAFVGGNITLLRGILYWIAQLLGSVVACLLLKFTTGGLVV-PAFGLSA 137

Query:   210 GLG 218
             G+G
Sbjct:   138 GVG 140


 >gi|8699604|gb|AAF78757.1|AF271660_1  (AF271660) putative aquaporin TIP3 [Vitis
            berlandieri x Vitis rupestris]
            Length = 251

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 306 (107.7 bits), Expect = 2.8e-26, P = 2.8e-26
 Identities = 62/122 (50%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG  T   +L+SGV     +V+EIV+TF L++TVYAT VDP
Sbjct:   107 WIAQLLGSVVACLLLKFSTGGLETSAFSLSSGVSVWNALVFEIVMTFGLVYTVYATAVDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             KKG L  + P  +GF+VGANILAGGA+  ASMNPA SFGP +  W          WA+HW
Sbjct:   167 KKGNLGIIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAVVSWS---------WANHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 116 (40.8 bits), Expect = 0.00019, P = 0.00019
 Identities = 17/31 (54%), Positives = 23/31 (74%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+ +W +HW YW GPLIG  +A  IY+
Sbjct:   205 GPAVVSWSWANHWVYWAGPLIGAAIAAIIYD 235


 >gi|5911732|emb|CAB55837.1|  (AJ245953) delta tonoplast intrinsic protein
            [Spinacia oleracea]
            Length = 247

Frame  3 hits (HSPs):                       ________________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |          |         |         |         | 247
                       0        50        100       150       200

  Plus Strand HSPs:

 Score = 305 (107.4 bits), Expect = 3.6e-26, P = 3.6e-26
 Identities = 61/122 (50%), Positives = 83/122 (68%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI Q   +  A  LL   +GG ATP+H++A+GVG  + VV EI++TF+L++TVYAT VDP
Sbjct:   104 WIAQCAGSVAACLLLKASTGGLATPIHSVAAGVGSIEAVVMEIIITFALVYTVYATAVDP 163

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             KKG++  + P  +GF+VGANILA G +S  SMNPARSFGP +    LD       ++ HW
Sbjct:   164 KKGSIGTIAPIAIGFIVGANILAAGPFSGGSMNPARSFGPAVA--SLD-------FSDHW 214

Query:   372 -WW 377
              +W
Sbjct:   215 VYW 217

 Score = 127 (44.7 bits), Expect = 1.7e-06, P = 1.7e-06
 Identities = 20/30 (66%), Positives = 26/30 (86%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIY 396
             GPA+ + +++DHW YWVGPL+GGGLAG IY
Sbjct:   202 GPAVASLDFSDHWVYWVGPLVGGGLAGLIY 231


 >gi|135860|sp|P25818|TIPG_ARATH  TONOPLAST INTRINSIC PROTEIN, GAMMA (GAMMA TIP)
            (AQUAPORIN-TIP) >gi|99761|pir||S22202 tonoplast intrinsic protein
            gamma - Arabidopsis thaliana >gi|16312|emb|CAA45115.1| (X63552)
            tonoplast intrinsic protein,  gamma-TIP(Ara). [Arabidopsis
            thaliana] >gi|166732|gb|AAA32806.1| (M84344) tonoplast intrinsic
            protein [Arabidopsis thaliana] >gi|4883600|gb|AAD31569.1|AC006922_1
            (AC006922) putative aquaporin (tonoplast intrinsic protein gamma)
            [Arabidopsis thaliana] >gi|445129|prf||1908432B tonoplast intrinsic
            protein gamma [Arabidopsis thaliana]
            Length = 251

Frame  3 hits (HSPs):     _________    _____________________________      
Frame  1 hits (HSPs):                                           ________  
Annotated Domains:      __________________________________________________
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250
__________________

Annotated Domains:
   BLOCKS               BL00221A: MIP family proteins.           25..35
   BLOCKS               BL00221B: MIP family proteins.           79..89
   BLOCKS               BL00221C: MIP family proteins.           148..164
   BLOCKS               BL00221D: MIP family proteins.           194..208
   BLOCKS               BL00221E: MIP family proteins.           216..226
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         1..24
   Entrez               Transmembrane region: POTENTIAL.         25..44
   Entrez               Domain: VACUOLAR (POTENTIAL).            45..57
   Entrez               Transmembrane region: POTENTIAL.         58..77
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         78..102
   Entrez               Transmembrane region: POTENTIAL.         103..121
   Entrez               Domain: VACUOLAR (POTENTIAL).            122..144
   Entrez               Transmembrane region: POTENTIAL.         145..164
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         165..170
   Entrez               Transmembrane region: POTENTIAL.         171..193
   Entrez               Domain: VACUOLAR (POTENTIAL).            194..216
   Entrez               Transmembrane region: POTENTIAL.         217..234
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         235..251
   PFAM                 MIP: Major intrinsic protein             13..234
   PRINTS               MINTRINSICP1: MIP family motif I - 3     21..40
   PRINTS               MINTRINSICP2: MIP family motif II - 3    65..89
   PRINTS               MINTRINSICP3: MIP family motif III - 3   102..121
   PRINTS               MINTRINSICP4: MIP family motif IV - 3    147..165
   PRINTS               MINTRINSICP5: MIP family motif V - 3     180..202
   PRINTS               MINTRINSICP6: MIP family motif VI - 3    217..237
   PRODOM               PD000295: GLPF(9) AQP1(5) AQP2(4)        20..233
   PROSITE              LEUCINE_ZIPPER: Leucine zipper pattern.  121..142
   PROSITE              MIP: MIP family signature.               83..91
__________________


  Plus Strand HSPs:

 Score = 304 (107.0 bits), Expect = 4.5e-26, P = 4.5e-26
 Identities = 59/122 (48%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  +L + +GG A P   L++GVG     V+EIV+TF L++TVYAT +DP
Sbjct:   107 WIAQLLGSVVACLILKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   167 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 146 (51.4 bits), Expect = 1.2e-12, Sum P(2) = 1.2e-12
 Identities = 22/33 (66%), Positives = 27/33 (81%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+  WT+HW YW GPL+GGG+AG IYE F
Sbjct:   205 GPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVF 237

 Score = 48 (16.9 bits), Expect = 1.2e-12, Sum P(2) = 1.2e-12
 Identities = 18/65 (27%), Positives = 29/65 (44%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFS-LLFTVYATMVDPKKGALAGLGPTLVGFV 239
             +SGG   P  T  + +G    ++  I+   + LL +V A ++   K A  GL     G  
Sbjct:    79 ISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI--LKFATGGLAVPAFGLS 136

Query:   240 VGANIL 257
              G  +L
Sbjct:   137 AGVGVL 142

 Score = 44 (15.5 bits), Expect = 3.2e-12, Sum P(2) = 3.2e-12
 Identities = 15/41 (36%), Positives = 21/41 (51%), Frame = +3

Query:   174 ATMVDPKKGALAGLGPTLVGFVVGANILAGGAYSAASMNPA 296
             AT  D  K ALA    TL+  V G+   +G A++  + N A
Sbjct:    14 ATRPDALKAALAEFISTLIFVVAGSG--SGMAFNKLTENGA 52


 >gi|475048|emb|CAA51171.1|  (X72581) tonoplast intrinsic protein gamma
            (gamma-TIP) [Arabidopsis thaliana]
            Length = 251

Frame  3 hits (HSPs):     _________    _____________________________      
Frame  1 hits (HSPs):                                           ________  
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 304 (107.0 bits), Expect = 4.5e-26, P = 4.5e-26
 Identities = 59/122 (48%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  +L + +GG A P   L++GVG     V+EIV+TF L++TVYAT +DP
Sbjct:   107 WIAQLLGSVVACLILKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   167 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 146 (51.4 bits), Expect = 1.2e-12, Sum P(2) = 1.2e-12
 Identities = 22/33 (66%), Positives = 27/33 (81%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+  WT+HW YW GPL+GGG+AG IYE F
Sbjct:   205 GPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVF 237

 Score = 48 (16.9 bits), Expect = 1.2e-12, Sum P(2) = 1.2e-12
 Identities = 18/65 (27%), Positives = 29/65 (44%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFS-LLFTVYATMVDPKKGALAGLGPTLVGFV 239
             +SGG   P  T  + +G    ++  I+   + LL +V A ++   K A  GL     G  
Sbjct:    79 ISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI--LKFATGGLAVPAFGLS 136

Query:   240 VGANIL 257
              G  +L
Sbjct:   137 AGVGVL 142

 Score = 44 (15.5 bits), Expect = 3.2e-12, Sum P(2) = 3.2e-12
 Identities = 15/41 (36%), Positives = 21/41 (51%), Frame = +3

Query:   174 ATMVDPKKGALAGLGPTLVGFVVGANILAGGAYSAASMNPA 296
             AT  D  K ALA    TL+  V G+   +G A++  + N A
Sbjct:    14 ATRPDALKAALAEFISTLIFVVAGSG--SGMAFNKLTENGA 52


 >gi|1514977|dbj|BAA12711.1|  (D84669) VM23 [Raphanus sativus]
            Length = 253

Frame  3 hits (HSPs):                  _____________________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 304 (107.0 bits), Expect = 4.5e-26, P = 4.5e-26
 Identities = 61/122 (50%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG A P   L++GV    G V+EIV+TF L++TVYAT VDP
Sbjct:   108 WIAQLLGSVVACLLLKFATGGLAVPAFGLSAGVESLNGFVFEIVMTFGLVYTVYATAVDP 167

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   168 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 218

Query:   372 -WW 377
              +W
Sbjct:   219 IYW 221

 Score = 141 (49.6 bits), Expect = 8.6e-12, Sum P(2) = 8.6e-12
 Identities = 22/31 (70%), Positives = 26/31 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+  WT+HW YW GPL+GGGLAG IYE
Sbjct:   206 GPAVVSWTWTNHWIYWAGPLVGGGLAGLIYE 236

 Score = 46 (16.2 bits), Expect = 8.6e-12, Sum P(2) = 8.6e-12
 Identities = 20/67 (29%), Positives = 29/67 (43%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFS-LLFTVYATMVDPKKGALAGLGPTLVGFV 239
             +SGG   P  T  + VG    ++  I+   + LL +V A ++   K A  GL     G  
Sbjct:    80 ISGGHVNPAVTFGAFVGGNITLLRGILYWIAQLLGSVVACLL--LKFATGGLAVPAFGLS 137

Query:   240 VGANILAG 263
              G   L G
Sbjct:   138 AGVESLNG 145


 >gi|5081419|gb|AAD39372.1|AF118381_1  (AF118381) tonoplast intrinsic protein
            [Brassica napus]
            Length = 253

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 304 (107.0 bits), Expect = 4.5e-26, P = 4.5e-26
 Identities = 61/122 (50%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG A P   L++GV    G V+EIV+TF L++TVYAT VDP
Sbjct:   108 WIAQLLGSVVACLLLKFATGGLAVPAFGLSAGVESLNGFVFEIVMTFGLVYTVYATAVDP 167

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   168 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 218

Query:   372 -WW 377
              +W
Sbjct:   219 IYW 221

 Score = 141 (49.6 bits), Expect = 1.6e-08, P = 1.6e-08
 Identities = 22/31 (70%), Positives = 26/31 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+  WT+HW YW GPL+GGGLAG IYE
Sbjct:   206 GPAVVSWTWTNHWIYWAGPLVGGGLAGLIYE 236


 >gi|7441269|pir||T14960  probable membrane intrinsic protein - parsley
            >gi|1743369|emb|CAA88267.1| (Z48232) putative membrane intrinsic
            protein [Petroselinum crispum]
            Length = 226

Frame  3 hits (HSPs):                          _______________________    
                        __________________________________________________
Database sequence:     |          |          |          |           |     | 226
                       0         50        100        150         200

  Plus Strand HSPs:

 Score = 303 (106.7 bits), Expect = 5.8e-26, P = 5.8e-26
 Identities = 54/102 (52%), Positives = 76/102 (74%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ A  AS+LL +++GG+A P+H + +G+G  +GVV+EI++TF+L++T YAT  DP
Sbjct:   105 WIAQLLGATVASFLLIFVNGGKAVPIHGVGAGLGAAEGVVFEIIITFALVYTFYATAADP 164

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCL 317
             KKG+L  + P  +GF+VGANILA G +S  SMNPAR  G  L
Sbjct:   165 KKGSLGTIAPIAIGFIVGANILAAGPFSGGSMNPARHSGQLL 206


 >gi|126962|sp|P21652|TIPR_ARATH  TONOPLAST INTRINSIC PROTEIN, ROOT-SPECIFIC RB7
            >gi|99747|pir||S13718 probable membrane channel protein RB7 -
            Arabidopsis thaliana >gi|16492|emb|CAA38633.1| (X54854) possible
            membrane channel protein [Arabidopsis thaliana]
            Length = 253

Frame  3 hits (HSPs):     _________    _____________________________      
Frame  1 hits (HSPs):                                           _______   
Annotated Domains:        _____________________________________________   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250
__________________

Annotated Domains:
   BLOCKS               BL00221A: MIP family proteins.           25..35
   BLOCKS               BL00221B: MIP family proteins.           79..89
   BLOCKS               BL00221C: MIP family proteins.           148..164
   BLOCKS               BL00221D: MIP family proteins.           194..208
   BLOCKS               BL00221E: MIP family proteins.           216..226
   DOMO                 DM00228: MIPFAMILY                       13..236
   PFAM                 MIP: Major intrinsic protein             13..234
   PRINTS               MINTRINSICP1: MIP family motif I - 3     21..40
   PRINTS               MINTRINSICP2: MIP family motif II - 3    65..89
   PRINTS               MINTRINSICP3: MIP family motif III - 3   102..121
   PRINTS               MINTRINSICP4: MIP family motif IV - 3    147..165
   PRINTS               MINTRINSICP5: MIP family motif V - 3     180..202
   PRINTS               MINTRINSICP6: MIP family motif VI - 3    217..237
   PRODOM               PD000295: GLPF(9) AQP1(5) AQP2(4)        20..233
   PROSITE              LEUCINE_ZIPPER: Leucine zipper pattern.  121..142
   PROSITE              MIP: MIP family signature.               83..91
__________________


  Plus Strand HSPs:

 Score = 302 (106.3 bits), Expect = 7.4e-26, P = 7.4e-26
 Identities = 59/122 (48%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  +L + +GG A P   L++GVG     V+EIV+TF L++TVYAT +DP
Sbjct:   107 WIAQLLGSVVACLILKFATGGLAVPPFGLSAGVGVLNAFVFEIVMTFGLVYTVYATAIDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   167 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 146 (51.4 bits), Expect = 2.1e-12, Sum P(2) = 2.1e-12
 Identities = 22/33 (66%), Positives = 27/33 (81%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+  WT+HW YW GPL+GGG+AG IYE F
Sbjct:   205 GPAVVSWTWTNHWVYWAGPLVGGGIAGLIYEVF 237

 Score = 46 (16.2 bits), Expect = 2.1e-12, Sum P(2) = 2.1e-12
 Identities = 18/65 (27%), Positives = 29/65 (44%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTFS-LLFTVYATMVDPKKGALAGLGPTLVGFV 239
             +SGG   P  T  + +G    ++  I+   + LL +V A ++   K A  GL     G  
Sbjct:    79 ISGGHVNPAVTFGAFIGGNITLLRGILYWIAQLLGSVVACLI--LKFATGGLAVPPFGLS 136

Query:   240 VGANIL 257
              G  +L
Sbjct:   137 AGVGVL 142

 Score = 44 (15.5 bits), Expect = 3.4e-12, Sum P(2) = 3.4e-12
 Identities = 15/41 (36%), Positives = 21/41 (51%), Frame = +3

Query:   174 ATMVDPKKGALAGLGPTLVGFVVGANILAGGAYSAASMNPA 296
             AT  D  K ALA    TL+  V G+   +G A++  + N A
Sbjct:    14 ATRPDALKAALAEFISTLIFVVAGSG--SGMAFNKLTENGA 52


 >gi|1928983|gb|AAB51394.1|  (U92652) tonoplast intrinsic protein bobTIP26-2
            [Brassica oleracea var. botrytis]
            Length = 175

Frame  3 hits (HSPs):   _________________________________________         
Frame  1 hits (HSPs):                                       __________    
                        __________________________________________________
Database sequence:     |              |             |             |       | 175
                       0             50           100           150

  Plus Strand HSPs:

 Score = 302 (106.3 bits), Expect = 7.4e-26, P = 7.4e-26
 Identities = 59/122 (48%), Positives = 79/122 (64%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG   P   L++GV     +V+EIV+TF L++TVYAT VDP
Sbjct:    31 WIAQLLGSVVACLLLKFATGGLVVPAFGLSAGVAVSNALVFEIVMTFGLVYTVYATAVDP 90

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K G+L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:    91 KNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWS---------WNNHW 141

Query:   372 -WW 377
              +W
Sbjct:   142 VYW 144

 Score = 142 (50.0 bits), Expect = 1.4e-11, Sum P(2) = 1.4e-11
 Identities = 22/33 (66%), Positives = 27/33 (81%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+ +W +HW YW GPLIGGG+AG IYE F
Sbjct:   129 GPAVVSWSWNNHWVYWAGPLIGGGVAGLIYEVF 161

 Score = 38 (13.4 bits), Expect = 1.4e-11, Sum P(2) = 1.4e-11
 Identities = 19/61 (31%), Positives = 28/61 (45%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYG----QGVV-WEIVLTFSL---LFTVYAT--MVDPKKGALAG 212
             +SGG   P  T  + VG      +G++ W   L  S+   L   +AT  +V P  G  AG
Sbjct:     3 ISGGHVNPAVTFGAFVGGNITLLRGILYWIAQLLGSVVACLLLKFATGGLVVPAFGLSAG 62

Query:   213 L 215
             +
Sbjct:    63 V 63


 >gi|541951|pir||JQ2288  SPCP2 protein - soybean >gi|310578|gb|AAA02947.1|
            (L12258) nodulin-26 [Glycine max]
            Length = 255

Frame  3 hits (HSPs):                ______________________________       
Frame  1 hits (HSPs):                                           _______   
Annotated Domains:        _____________________________________________   
                        __________________________________________________
Database sequence:     |         |         |         |         |        | | 255
                       0        50       100       150       200      250
__________________

Annotated Domains:
   DOMO                 DM00228: MIPFAMILY                       13..236
   Entrez               domain: transmembrane                    24..43
   Entrez               domain: transmembrane                    54..76
   Entrez               domain: transmembrane                    103..121
   Entrez               domain: transmembrane                    144..163
   Entrez               domain: transmembrane                    173..193
   Entrez               domain: transmembrane                    217..234
   PROSITE              MIP: MIP family signature.               83..91
__________________


  Plus Strand HSPs:

 Score = 302 (106.3 bits), Expect = 7.4e-26, P = 7.4e-26
 Identities = 61/122 (50%), Positives = 79/122 (64%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A  LL + +GG  T    L+ GV  G  +V+EIV+TF L++TVYAT VDP
Sbjct:   107 WIAQLLGSVVACLLLKFATGGLETSAFALSPGVEAGNALVFEIVMTFGLVYTVYATAVDP 166

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             KKG L  + P  +GF+VGANILAGGA+  ASMNPA SFGP +  W          W++HW
Sbjct:   167 KKGDLGIIAPIAIGFIVGANILAGGAFDGASMNPAVSFGPAVVSWT---------WSNHW 217

Query:   372 -WW 377
              +W
Sbjct:   218 VYW 220

 Score = 118 (41.5 bits), Expect = 7.5e-07, Sum P(2) = 7.5e-07
 Identities = 17/33 (51%), Positives = 23/33 (69%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYETF 405
             GPA+V+  W++HW YWVGP  G  +A  +YE F
Sbjct:   205 GPAVVSWTWSNHWVYWVGPFAGAAIAAIVYEIF 237

 Score = 47 (16.5 bits), Expect = 7.5e-07, Sum P(2) = 7.5e-07
 Identities = 21/69 (30%), Positives = 33/69 (47%), Frame = +3

Query:    63 LSGGQATPVHTLASGVGYGQGVVWEIVLTF--SLLFTVYATMVDPKKGALAGLGPTLVGF 236
             +SGG   P  T  + VG G   ++  +L +   LL +V A ++   K A  GL  +   F
Sbjct:    79 ISGGHVNPAVTFGAFVG-GHITLFRSILYWIAQLLGSVVACLL--LKFATGGLETS--AF 133

Query:   237 VVGANILAGGA 269
              +   + AG A
Sbjct:   134 ALSPGVEAGNA 144

 Score = 43 (15.1 bits), Expect = 1.9e-06, Sum P(2) = 1.9e-06
 Identities = 14/31 (45%), Positives = 18/31 (58%), Frame = +3

Query:   228 VGFVVGANILAGGAYSAASMNPARSFGPCLG 320
             V   VGANI +GG      +NPA +FG  +G
Sbjct:    71 VAVSVGANI-SGG-----HVNPAVTFGAFVG 95


 >gi|7340919|dbj|BAA92991.1|  (AP001550) ESTs C91632(E31121),AU029591(E31121)
            correspond to a region of the predicted gene.; Similar to
            Arabidopsis thaliana chromosome II BAC F17H15 genomic sequence;
            putative tonoplast intrinsic protein (AC005395) [Oryza sativa]
            Length = 251

Frame  3 hits (HSPs):    __________________  _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 299 (105.3 bits), Expect = 1.5e-25, P = 1.5e-25
 Identities = 68/119 (57%), Positives = 80/119 (67%), Frame = +3

Query:    21 QLVAAATASYLLYYLSGGQ-ATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPKK 197
             QL+ ++ A  LL  L+GG+ A PVH  A GVG  + V  E VLTFSLLF VYAT+VD ++
Sbjct:   107 QLLGSSLACLLLAALTGGEEAVPVHAPAPGVGAARAVAMEAVLTFSLLFAVYATVVDRRR 166

Query:   198 GALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW-W 374
              A+  LGP LVG VVGANILAGG YS ASMNPARSFGP L   +         WA HW +
Sbjct:   167 -AVGALGPLLVGLVVGANILAGGPYSGASMNPARSFGPALAAGE---------WADHWIY 216

Query:   375 W 377
             W
Sbjct:   217 W 217

 Score = 147 (51.7 bits), Expect = 1.5e-14, Sum P(2) = 1.5e-14
 Identities = 24/31 (77%), Positives = 25/31 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPAL AG W DHW YWVGPLIGG LAG +YE
Sbjct:   202 GPALAAGEWADHWIYWVGPLIGGPLAGLVYE 232

 Score = 65 (22.9 bits), Expect = 1.5e-14, Sum P(2) = 1.5e-14
 Identities = 26/84 (30%), Positives = 39/84 (46%), Frame = +3

Query:    90 HTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPKKGALAGLGPTLVGF---------VV 242
             H  A+  G  + VV E++LTF  +F+   + +   K  L G G T++G          VV
Sbjct:     9 HREATDPGCLRAVVAELLLTFLFVFSGVGSAMAAAK--LGGGGDTIMGLTAVAAAHALVV 66

Query:   243 GANILAGGAYSAASMNPARSFGPCLG 320
                + AG   S   +NPA + G   G
Sbjct:    67 AVMVSAGLHVSGGHINPAVTLGLAAG 92

 Score = 39 (13.7 bits), Expect = 7.7e-12, Sum P(2) = 7.7e-12
 Identities = 12/30 (40%), Positives = 14/30 (46%), Frame = +3

Query:    24 LVAAATASYLLYYLSGGQATPVHTLASGVG 113
             LV A   S  L+ +SGG   P  TL    G
Sbjct:    64 LVVAVMVSAGLH-VSGGHINPAVTLGLAAG 92


 >gi|9082285|gb|AAF82790.1|AF275315_1  (AF275315) water-selective transport
            intrinsic membrane protein 1; LIMP1 [Lotus japonicus]
            Length = 251

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 251
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 297 (104.5 bits), Expect = 2.5e-25, P = 2.5e-25
 Identities = 61/121 (50%), Positives = 81/121 (66%), Frame = +3

Query:    15 IDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDPK 194
             I QL+ +  AS LL +++G  A P   L++GVG G  +V EIV+TF L++TVYAT VDPK
Sbjct:   108 IAQLLGSIVASLLLAFVTG-LAVPAFGLSAGVGVGNALVLEIVMTFGLVYTVYATAVDPK 166

Query:   195 KGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW- 371
             KG+L  + P  +GF+VGANIL GGA+  ASMNPA SFGP +  W          W++HW 
Sbjct:   167 KGSLGTIAPIAIGFIVGANILLGGAFDGASMNPAVSFGPAVVSWS---------WSNHWI 217

Query:   372 WW 377
             +W
Sbjct:   218 YW 219

 Score = 140 (49.3 bits), Expect = 2.0e-08, P = 2.0e-08
 Identities = 21/31 (67%), Positives = 28/31 (90%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+ +W++HW YWVGPL+GGG+AG IYE
Sbjct:   204 GPAVVSWSWSNHWIYWVGPLVGGGIAGLIYE 234


 >gi|11357300|pir||T51819  gamma tonoplast intrinsic protein 2 [imported] -
            Arabidopsis thaliana >gi|3688799|gb|AAC62397.1| (AF057137) gamma
            tonoplast intrinsic protein 2 [Arabidopsis thaliana]
            Length = 253

Frame  3 hits (HSPs):                        _______________________      
Frame  1 hits (HSPs):                                           _______   
                        __________________________________________________
Database sequence:     |         |         |         |         |         || 253
                       0        50       100       150       200       250

  Plus Strand HSPs:

 Score = 296 (104.2 bits), Expect = 3.2e-25, P = 3.2e-25
 Identities = 58/122 (47%), Positives = 80/122 (65%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL + +GG+  P   L++GV     +V+EIV+TF L++TVYAT VDP
Sbjct:   108 WIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVRSLNALVFEIVMTFGLVYTVYATAVDP 167

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             K  +L  + P  +GF+VGANILAGGA+S ASMNPA +FGP +  W          W +HW
Sbjct:   168 KNASLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWT---------WTNHW 218

Query:   372 -WW 377
              +W
Sbjct:   219 VYW 221

 Score = 140 (49.3 bits), Expect = 2.1e-08, P = 2.1e-08
 Identities = 22/31 (70%), Positives = 26/31 (83%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPA+V+  WT+HW YW GPLIGGGLAG IY+
Sbjct:   206 GPAVVSWTWTNHWVYWAGPLIGGGLAGIIYD 236


 >gi|1729971|sp|P50156|TIPG_ORYSA  TONOPLAST INTRINSIC PROTEIN, GAMMA (GAMMA TIP)
            (AQUAPORIN-TIP) >gi|1076745|pir||S52004 gamma-Tip protein - rice
            >gi|473997|dbj|BAA05017.1| (D25534) gamma-Tip [Oryza sativa]
            Length = 250

Frame  3 hits (HSPs):              ________  _______________________      
Frame  1 hits (HSPs):                                           _______   
Annotated Domains:      __________________________________________________
                        __________________________________________________
Database sequence:     |         |         |         |         |          | 250
                       0        50       100       150       200
__________________

Annotated Domains:
   BLOCKS               BL00221A: MIP family proteins.           25..35
   BLOCKS               BL00221B: MIP family proteins.           79..89
   BLOCKS               BL00221C: MIP family proteins.           147..163
   BLOCKS               BL00221D: MIP family proteins.           193..207
   BLOCKS               BL00221E: MIP family proteins.           215..225
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         1..24
   Entrez               Transmembrane region: POTENTIAL.         25..44
   Entrez               Domain: VACUOLAR (POTENTIAL).            45..57
   Entrez               Transmembrane region: POTENTIAL.         58..77
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         78..102
   Entrez               Transmembrane region: POTENTIAL.         103..121
   Entrez               Domain: VACUOLAR (POTENTIAL).            122..143
   Entrez               Transmembrane region: POTENTIAL.         144..163
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         164..169
   Entrez               Transmembrane region: POTENTIAL.         170..192
   Entrez               Domain: VACUOLAR (POTENTIAL).            193..215
   Entrez               Transmembrane region: POTENTIAL.         216..233
   Entrez               Domain: CYTOPLASMIC (POTENTIAL).         234..250
   PFAM                 MIP: Major intrinsic protein             13..233
   PRINTS               MINTRINSICP1: MIP family motif I - 3     21..40
   PRINTS               MINTRINSICP2: MIP family motif II - 3    65..89
   PRINTS               MINTRINSICP3: MIP family motif III - 3   102..121
   PRINTS               MINTRINSICP4: MIP family motif IV - 3    146..164
   PRINTS               MINTRINSICP5: MIP family motif V - 3     179..201
   PRINTS               MINTRINSICP6: MIP family motif VI - 3    216..236
   PRODOM               PD000295: GLPF(9) AQP1(5) AQP2(4)        20..232
   PROSITE              MIP: MIP family signature.               83..91
__________________


  Plus Strand HSPs:

 Score = 291 (102.4 bits), Expect = 1.1e-24, P = 1.1e-24
 Identities = 61/122 (50%), Positives = 78/122 (63%), Frame = +3

Query:    12 WIDQLVAAATASYLLYYLSGGQATPVHTLASGVGYGQGVVWEIVLTFSLLFTVYATMVDP 191
             WI QL+ +  A +LL + +GG AT    L +GV   + +V EIV+TF L++TVYAT VDP
Sbjct:   107 WIAQLLGSTVACFLLRFSTGGLATGTFGL-TGVSVWEALVLEIVMTFGLVYTVYATAVDP 165

Query:   192 KKGALAGLGPTLVGFVVGANILAGGAYSAASMNPARSFGPCLGRWKLDRSLGLLGWASHW 371
             KKG+L  + P  +GF+VGANIL GGA+  ASMNPA SFGP L  W          W S W
Sbjct:   166 KKGSLGTIAPIAIGFIVGANILVGGAFDGASMNPAVSFGPALVSWS---------WESQW 216

Query:   372 -WW 377
              +W
Sbjct:   217 VYW 219

 Score = 136 (47.9 bits), Expect = 2.7e-10, Sum P(2) = 2.7e-10
 Identities = 23/31 (74%), Positives = 25/31 (80%), Frame = +1

Query:   307 GPALVAGNWTDHWAYWVGPLIGGGLAGYIYE 399
             GPALV+ +W   W YWVGPLIGGGLAG IYE
Sbjct:   204 GPALVSWSWESQWVYWVGPLIGGGLAGVIYE 234

 Score = 37 (13.0 bits), Expect = 2.7e-10, Sum P(2) = 2.7e-10
 Identities = 13/38 (34%), Positives = 19/38 (50%), Frame = +3

Query:    24 LVAAATA-SYLLYY-------LSGGQATPVHTLASGVG 113
             L+AAA A ++ L+        +SGG   P  T  + VG
Sbjct:    58 LIAAAVAHAFALFVAVSVGANISGGHVNPAVTFGAFVG 95


WARNING:  HSPs involving 325 database sequences were not reported due to the
          limiting value of parameter B = 50.


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.95

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.340   0.149   0.528  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.335   0.146   0.541  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.352   0.160   0.630  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.336   0.146   0.481  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.345   0.152   0.561  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.344   0.147   0.496  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0      176       174       10.  75 3  12 22  0.11    34
                                                    31  0.11    37
   +2      0      176       175       10.  75 3  12 22  0.11    34
                                                    31  0.11    37
   +1      0      177       176       10.  75 3  12 22  0.11    34
                                                    31  0.11    37
   -1      0      177       175       10.  75 3  12 22  0.11    34
                                                    31  0.11    37
   -2      0      176       174       10.  75 3  12 22  0.11    34
                                                    31  0.11    37
   -3      0      176       174       10.  75 3  12 22  0.11    34
                                                    31  0.11    37


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  4:06 PM CST Feb 28, 2001
    Format:  BLAST
  # of letters in database:  197,782,623
  # of sequences in database:  625,274
  # of database sequences satisfying E:  375
  No. of states in DFA:  593 (58 KB)
  Total size of DFA:  224 KB (256 KB)
  Time to generate neighborhood:  0.01u 0.01s 0.02t  Elapsed: 00:00:00
  No. of threads or processors used:  6
  Search cpu time:  204.76u 1.20s 205.96t  Elapsed: 00:01:41
  Total cpu time:  204.83u 1.24s 206.07t  Elapsed: 00:01:41
  Start:  Wed Jan 23 19:11:12 2002   End:  Wed Jan 23 19:12:53 2002

WARNINGS ISSUED:  2


Annotated Domains Database:   March 14, 2000 

Release Date:  March 14, 2000