KHURANA CM, EIBEN GL, WELDON-LINNE CM; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 688 (abstract no. 1743).

Illinois Masonic Med. Ctr., Chicago, IL.

There is an urgent demand for a sensitive, rapid, accurate and noninvasive screening test for the detection of genital infetions in women. With the development of automated polymerase and ligase chain reaction (PCR, LCR) assays, it is possible to screen for Ct and Ng infections from one source, either urine or urogenital swab. Endocervical specimens do not usually contain microorganisms from an infected urethra, whereas urine from women usually contains microorganisms from both the urethra and cervix. We conducted an evaluation of the Abbott LCx (LCR) and the Roche COBAS AMPLICOR (PCR) assay for the detection of Ct and Ng on first void urine (FVU) and compared the results with those obtained from endocervical swabs (ECS). FVU and ECS were obtained from 166 women visiting 5 outpatient clinics and our ER. PCR and LCR were performed on fresh and frozen FVU and the corresponding swab transport media. Discrepant results were repeated in duplicate and then confirmed by either the MOMP assay for Ct or the pilin B probe assay for Ng. The prevalence of Ct was 7% and of Ng 3%. Ct sensitivity on the COBAS was 63.6% for FVU and 54.5% for ECS. Specificity was 100% for both sites. Ct sensitivity on the LCx was 91% for FVU and 54.5% for ECS. Specificity was 99.3% for FVU and 100% for ECS. 36% of positivie infections were confined to the urethra, while only 9% were confined to the cervix. Both sites were positive 54% of the time. Ng sensitivity on the COBAS for FVU was 40% and 80% for ECS. Specificity was 100% and 99.7% respectively. Ng sensitivity on the LCx was 80% for ECS. Specificity was 100% and 99.7% respectively. Ng sensitivity on the LCx was 80% for urine, 100% for ECS and specificity was 100% for both sites. 20% of Ng infections were confined to the cervix, while Ng was never confined to the urethra. Infection was detected from both sites 80% of the time. In conclusion, the LC x was more sensitive that the COBAS in the detection of Ct and Ng on FVU. LCx FVU confimed the greatest number of Ct infections, isolating those confined to the urethra as well as cervix. These results show that FVU is a reliable and noninvasive method which can replace urethral swabs in the screening of Ct and infection in women.

Publication Types:

• Meeting Abstracts

Keywords:

• Bacteriological Techniques
• Cervix Uteri
• Chlamydia trachomatis
• Evaluation Studies
• Female
• Humans
• Ligase Chain Reaction
• Male
• Neisseria gonorrhoeae
• Polymerase Chain Reaction
• Sensitivity and Specificity
• Urethra
• Urogenital System
• methods
• urine

Other ID:

• GWAIDS0008827

UI: 102246324

From Meeting Abstracts