Villinger F, Brice GT, Mayne AE, Bostik P, Mori K, June CH, Ansari AA.
AIDS Vaccine 2001. 2001 Sep 5-8; abstract no. 163.
Dept of Pathology, Emory University of Medicine, Atlanta GA,
HAART chemotherapy has significantly enhanced survival of HIV-1 infected patients, yet such therapy falls short of eradicating the infection and most often requires lifelong treatment, causes significant side effects and only protracted immune recovery. Thus, the need for adjunct immune enhancing strategies is clearly warranted. Adoptive immunotherapy was performed in SIVmac239 infected monkeys following short term antiviral therapy with PMPA. While PMPA chemotherapy alone led to only transient decreases in plasma and cellular proviral DNA loads and transient rescue of gag/pol and env pCTL, PMPA therapy coupled with infusions of either autologous pre-SIV infection collected PBMC or activated CD4+ T cells led to control of plasma and cellular proviral DNA loads for several months post infusion, despite the fact that the infused PBMCs or activated CD4+ T cells were naive for SIV. However, the predominant mechanisms by which control of viral replication was achieved did apparently differ between monkeys given autologous unfractionated PBMCs versus those given activated CD4+ T cells: PBMC infusions significantly favored development of SIVenv pCTL, neutralizing antibodies, and secretion of soluble non-cytotoxic suppressor factors of SIV replication. In contrast, activated CD4+ T cells predominantly promoted CTL responses to SIVgag/pol and env. In addition, infusion of influenza primed activated CD4+ T cells markedly enhanced influenza specific pCTL responses while infusion of similarly influenza primed unfractionated PBMC enhanced such pCTL responses only modestly.Nevertheless, this report clearly demonstrate that adoptive immunotherapy with autologous purified SIV naive CD4+ lymphocytes was sufficient to induce long term control of SIV replication in the absence of continued antiviral chemotherapy.Supported by grants from AMFAR 97-102009, NIH-1RO1-27057 and AIDS research grants from the Health Science Foundation in Japan, Ministry
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Adenine
- Animals
- Antigens, CD4
- CD4-Positive T-Lymphocytes
- Communicable Diseases
- Genes, gag
- Humans
- Immunotherapy, Adoptive
- Infection
- Influenza Vaccines
- Japan
- Macaca mulatta
- Phosphonic Acids
- Simian Acquired Immunodeficiency Syndrome
- Simian immunodeficiency virus
- T-Lymphocytes, Cytotoxic
- genetics
- immunology
- tenofovir
Other ID:
UI: 102249181
From Meeting Abstracts