R = Retrieve links to Related sequences (neighbors).
Use the 
icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the 
icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the 
icon (if present) to retrieve links to the Protein Data Bank database.
WARNING: Descriptions of 80 database sequences were not reported due to the
limiting value of parameter V = 50.
>gi|4314390|gb|AAD15600.1| (AC006232) putative Ca2+-binding protein
[Arabidopsis thaliana]
Length = 186
Frame 2 hits (HSPs): _________
Frame 1 hits (HSPs): ____________ ____________________________
__________________________________________________
Database sequence: | | | | | 186
0 50 100 150
Plus Strand HSPs:
Score = 305 (107.4 bits), Expect = 3.6e-26, P = 3.6e-26
Identities = 56/101 (55%), Positives = 75/101 (74%), Frame = +1
Query: 145 PKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDG 324
PKE+ LW+CL WR IF RY +DRSGK++ +LRDA Y +G +P SV QL++S++ DG
Sbjct: 71 PKEYVELWNCLAQWRAIFNRYDRDRSGKMNSTQLRDAFYNLGCVLPTSVHQLIVSQFDDG 130
Query: 325 SGRRVELGFDSFVECGMIIKGLTDKFKGE--GYTVYG-FSY 438
+G+ V+L FDSF+ECGMI+KGLT+KF+ GYT Y SY
Sbjct: 131 TGKTVDLCFDSFLECGMIVKGLTEKFRENDPGYTGYATLSY 171
Score = 109 (38.4 bits), Expect = 3.1e-06, Sum P(2) = 3.1e-06
Identities = 20/30 (66%), Positives = 23/30 (76%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMVLPFLVSYD 493
RE D YTG ATLSYD FM MV+PF+ +YD
Sbjct: 157 RENDPGYTGYATLSYDVFMLMVIPFIATYD 186
Score = 59 (20.8 bits), Expect = 3.1e-06, Sum P(2) = 3.1e-06
Identities = 16/41 (39%), Positives = 23/41 (56%), Frame = +1
Query: 196 FERYXKDRSGKIDPLELRDALYGIGY-AVPGSVLQLLLSKY 315
FE ++RSG ++ ELR AL GY + ++LLL Y
Sbjct: 14 FESADRNRSGFLEESELRQALSLSGYDGISNRTIRLLLFIY 54
>gi|6624721|emb|CAB63845.1| (AJ251828) putative cysteine protease [Pisum
sativum]
Length = 286
Frame 1 hits (HSPs): ___________________
__________________________________________________
Database sequence: | | | | | | | 286
0 50 100 150 200 250
Plus Strand HSPs:
Score = 270 (95.0 bits), Expect = 1.8e-22, P = 1.8e-22
Identities = 53/105 (50%), Positives = 72/105 (68%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
GPKEF +L+ L WRGIFER+ KDRSG+ID ELRDAL +GYAV +VL LL+SK+
Sbjct: 181 GPKEFTSLFYSLQSWRGIFERFDKDRSGQIDSNELRDALLSLGYAVSPTVLDLLVSKFDK 240
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYGFSYTFIRRL 456
G+ + +D+F+EC + +KGLTDKFK + + + +RRL
Sbjct: 241 TGGKHKAVEYDNFIECCLTVKGLTDKFKEKDTGILALQHFPMRRL 285
>gi|9955572|emb|CAC05499.1| (AL391716) EF-hand Calcium binding protein-like
[Arabidopsis thaliana]
Length = 354
Frame 2 hits (HSPs): ____
Frame 1 hits (HSPs): _______________
__________________________________________________
Database sequence: | | | | 354
0 150 300
Plus Strand HSPs:
Score = 258 (90.8 bits), Expect = 3.4e-21, P = 3.4e-21
Identities = 53/101 (52%), Positives = 69/101 (68%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
GPKEF AL+ L +WR IFER KDRSG+ID ELRDAL +G++V VL LL+SK+
Sbjct: 240 GPKEFTALFYSLQNWRSIFERSDKDRSGRIDVNELRDALLSLGFSVSPVVLDLLVSKFDK 299
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYGFSYTF 444
G+ + +D+F+EC + +KGLT+KFK E T Y S TF
Sbjct: 300 SGGKNRAIEYDNFIECCLTVKGLTEKFK-EKDTAYSGSATF 339
Score = 98 (34.5 bits), Expect = 0.26, P = 0.23
Identities = 17/28 (60%), Positives = 24/28 (85%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMVLPFLVS 487
+EKDT Y+GSAT +Y++FM VLPFL++
Sbjct: 327 KEKDTAYSGSATFNYESFMLTVLPFLIA 354
>gi|6056209|gb|AAF02826.1|AC009400_22 (AC009400) unknown protein [Arabidopsis
thaliana]
Length = 330
Frame 1 hits (HSPs): ____________
__________________________________________________
Database sequence: | | | | 330
0 150 300
Plus Strand HSPs:
Score = 202 (71.1 bits), Expect = 4.9e-15, P = 4.9e-15
Identities = 38/75 (50%), Positives = 53/75 (70%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
GPKEF +L+ L +WR IFER+ KDRSG+ID ELRDAL +G++V +L LL+SK+
Sbjct: 221 GPKEFTSLFFSLQNWRSIFERFDKDRSGRIDTNELRDALMSLGFSVSPVILDLLVSKFDK 280
Query: 322 GSGRRVELGFDSFVE 366
GR + +D+F+E
Sbjct: 281 SGGRNRAIEYDNFIE 295
>gi|90342|pir||S04970 calcium-binding protein (clone pMP41) - mouse (fragment)
>gi|50266|emb|CAA33064.1| (X14938) put. calcium-binding protein
(153 AA, C-term.) [Mus musculus]
Length = 153
Frame 2 hits (HSPs): ________
Frame 1 hits (HSPs): ____________________________
Annotated Domains: ____________ _____
__________________________________________________
Database sequence: | | | | | 153
0 50 100 150
__________________
Annotated Domains:
Entrez domain: calmodulin repeat homology #labe 22..54
PROSITE EF_HAND: EF-hand calcium-binding domain. 65..77
__________________
Plus Strand HSPs:
Score = 155 (54.6 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 30/85 (35%), Positives = 49/85 (57%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF +W + W+ +F Y +D SG ID EL+ AL G GY + +L+ K+ D G
Sbjct: 46 EFTGVWKYITDWQNVFRTYDRDNSGMIDKNELKQALSGFGYRLSDQFHDILIRKF-DRQG 104
Query: 331 RRVELGFDSFVECGMIIKGLTDKFK 405
R ++ FD F++ ++++ LTD F+
Sbjct: 105 RG-QIAFDDFIQGCIVLQRLTDIFR 128
Score = 39 (13.7 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 8/22 (36%), Positives = 13/22 (59%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMV 469
R DT G +SY+ +++MV
Sbjct: 128 RRYDTDQDGWIQVSYEQYLSMV 149
>gi|6755000 ref|NP_035181.1| programmed cell death 6 [Mus musculus]
>gi|2506252|sp|P12815|PCD6_MOUSE PROGRAMED CELL DEATH PROTEIN 6
(PROBABLE CALCIUM-BINDING PROTEIN ALG-2) (PMP41) (ALG-257)
>gi|1213520|gb|AAB38108.1| (U49112) ALG-2 [Mus musculus]
Length = 191
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): _______________________
__________________________________________________
Database sequence: | | | | | 191
0 50 100 150
Plus Strand HSPs:
Score = 155 (54.6 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 30/85 (35%), Positives = 49/85 (57%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF +W + W+ +F Y +D SG ID EL+ AL G GY + +L+ K+ D G
Sbjct: 84 EFTGVWKYITDWQNVFRTYDRDNSGMIDKNELKQALSGFGYRLSDQFHDILIRKF-DRQG 142
Query: 331 RRVELGFDSFVECGMIIKGLTDKFK 405
R ++ FD F++ ++++ LTD F+
Sbjct: 143 RG-QIAFDDFIQGCIVLQRLTDIFR 166
Score = 39 (13.7 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 8/22 (36%), Positives = 13/22 (59%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMV 469
R DT G +SY+ +++MV
Sbjct: 166 RRYDTDQDGWIQVSYEQYLSMV 187
>gi|7019485 ref|NP_037364.1| programmed cell death 6 [Homo sapiens]
>gi|12230420|sp|O75340|PCD6_HUMAN PROGRAMED CELL DEATH PROTEIN 6
(PROBABLE CALCIUM-BINDING PROTEIN ALG-2) >gi|3342794|gb|AAC27697.1|
(AF035606) calcium binding protein [Homo sapiens]
>gi|6502505|gb|AAF14336.1|U58773_1 (U58773) calcium binding protein
[Homo sapiens]
Length = 191
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): _______________________
__________________________________________________
Database sequence: | | | | | 191
0 50 100 150
Plus Strand HSPs:
Score = 155 (54.6 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 30/85 (35%), Positives = 49/85 (57%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF +W + W+ +F Y +D SG ID EL+ AL G GY + +L+ K+ D G
Sbjct: 84 EFTGVWKYITDWQNVFRTYDRDNSGMIDKNELKQALSGFGYRLSDQFHDILIRKF-DRQG 142
Query: 331 RRVELGFDSFVECGMIIKGLTDKFK 405
R ++ FD F++ ++++ LTD F+
Sbjct: 143 RG-QIAFDDFIQGCIVLQRLTDIFR 166
Score = 39 (13.7 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 8/22 (36%), Positives = 13/22 (59%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMV 469
R DT G +SY+ +++MV
Sbjct: 166 RRYDTDQDGWIQVSYEQYLSMV 187
>gi|12731702 ref|XP_004040.2| programmed cell death 6 [Homo sapiens]
Length = 201
Frame 2 hits (HSPs): ______
Frame 1 hits (HSPs): _____________________
__________________________________________________
Database sequence: | | | | || 201
0 50 100 150 200
Plus Strand HSPs:
Score = 155 (54.6 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 30/85 (35%), Positives = 49/85 (57%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF +W + W+ +F Y +D SG ID EL+ AL G GY + +L+ K+ D G
Sbjct: 94 EFTGVWKYITDWQNVFRTYDRDNSGMIDKNELKQALSGFGYRLSDQFHDILIRKF-DRQG 152
Query: 331 RRVELGFDSFVECGMIIKGLTDKFK 405
R ++ FD F++ ++++ LTD F+
Sbjct: 153 RG-QIAFDDFIQGCIVLQRLTDIFR 176
Score = 39 (13.7 bits), Expect = 1.1e-12, Sum P(2) = 1.1e-12
Identities = 8/22 (36%), Positives = 13/22 (59%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMV 469
R DT G +SY+ +++MV
Sbjct: 176 RRYDTDQDGWIQVSYEQYLSMV 197
>gi|6912582 ref|NP_036524.1| peflin [Homo sapiens] >gi|6009487|dbj|BAA84922.1|
(AB018357) ABP32 [Homo sapiens] >gi|6015440|dbj|BAA85163.1|
(AB026628) peflin [Homo sapiens] >gi|7022666|dbj|BAA91680.1|
(AK001420) unnamed protein product [Homo sapiens]
Length = 284
Frame 2 hits (HSPs): ____
Frame 1 hits (HSPs): _________________
__________________________________________________
Database sequence: | | | | | | | 284
0 50 100 150 200 250
Plus Strand HSPs:
Score = 144 (50.7 bits), Expect = 2.8e-09, Sum P(2) = 2.8e-09
Identities = 31/92 (33%), Positives = 51/92 (55%), Frame = +1
Query: 154 FAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSGR 333
F+ALW + W+ +F++Y +DRSG I EL+ AL +GY + QLL+S+Y S
Sbjct: 176 FSALWKFIQQWKNLFQQYDRDRSGSISYTELQQALSQMGYNLSPQFTQLLVSRYCPRSAN 235
Query: 334 RVELGFDSFVECGMIIKGLTDKFKGEGYTVYG 429
+ D F++ ++ LT+ F+ + V G
Sbjct: 236 PA-MQLDRFIQVCTQLQVLTEAFREKDTAVQG 266
Score = 56 (19.7 bits), Expect = 2.8e-09, Sum P(2) = 2.8e-09
Identities = 11/21 (52%), Positives = 15/21 (71%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTM 466
REKDT G+ LS++ F+TM
Sbjct: 258 REKDTAVQGNIRLSFEDFVTM 278
>gi|4507207 ref|NP_003121.1| sorcin; Sorcin (class 4 gene) [Homo sapiens]
>gi|11419205 ref|XP_004593.1| sorcin [Homo sapiens]
>gi|267021|sp|P30626|SORC_HUMAN SORCIN (22 KD PROTEIN) (CP-22)
(V19) >gi|1082793|pir||S52094 sorcin - human
>gi|2136183|pir||S68369 sorcin - human >gi|338482|gb|AAA60588.1|
(M32886) sorcin CP-22 [Homo sapiens] >gi|459836|gb|AAA92155.1|
(L12387) sorcin [Homo sapiens] >gi|1094394|prf||2106141A sorcin
[Homo sapiens]
Length = 198
Frame 2 hits (HSPs): _______
Frame 1 hits (HSPs): ______________________
__________________________________________________
Database sequence: | | | | | 198
0 50 100 150
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 5.8e-09, Sum P(2) = 5.8e-09
Identities = 29/88 (32%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G EF LW+ L WR F + DRSG +DP EL+ AL +G+ + + + +Y
Sbjct: 91 GFNEFKELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIAKRYST 150
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
+G+ + FD ++ C + ++ LTD F+
Sbjct: 151 -NGK---ITFDDYIACCVKLRALTDSFR 174
Score = 40 (14.1 bits), Expect = 5.8e-09, Sum P(2) = 5.8e-09
Identities = 8/23 (34%), Positives = 11/23 (47%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMVL 472
R +DT G YD F+ V+
Sbjct: 174 RRRDTAQQGVVNFPYDDFIQCVM 196
>gi|1587138|prf||2206278A sorcin [Homo sapiens]
Length = 198
Frame 2 hits (HSPs): _______
Frame 1 hits (HSPs): ______________________
__________________________________________________
Database sequence: | | | | | 198
0 50 100 150
Plus Strand HSPs:
Score = 139 (48.9 bits), Expect = 5.8e-09, Sum P(2) = 5.8e-09
Identities = 29/88 (32%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G EF LW+ L WR F + DRSG +DP EL+ AL +G+ + + + +Y
Sbjct: 91 GFNEFCELWAVLNGWRQHFISFDTDRSGTVDPQELQKALTTMGFRLSPQAVNSIACRYST 150
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
+G+ + FD ++ C + ++ LTD F+
Sbjct: 151 -NGK---ITFDDYIACCVKLRALTDSFR 174
Score = 40 (14.1 bits), Expect = 5.8e-09, Sum P(2) = 5.8e-09
Identities = 8/23 (34%), Positives = 11/23 (47%), Frame = +2
Query: 404 REKDTRYTGSATLSYDAFMTMVL 472
R +DT G YD F+ V+
Sbjct: 174 RRRDTAQQGVVNFPYDDFIQCVM 196
>gi|134734|sp|P05044|SORC_CRILO SORCIN (22 KD PROTEIN) (CP-22) (V19)
>gi|90213|pir||A25706 sorcin - Chinese hamster
>gi|49528|emb|CAA28354.1| (X04656) 22-kd protein (AA 1-198)
[Cricetulus longicaudatus]
Length = 198
Frame 1 hits (HSPs): ___________________________
Annotated Domains: ________________________________________________
__________________________________________________
Database sequence: | | | | | 198
0 50 100 150
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 83..95
DOMO DM01246: CALPAINCATALYTICDOMAIN 12..51
DOMO DM00011: CALMODULINREPEAT 52..95
DOMO DM01221: CALPAINCATALYTICDOMAIN 97..196
Entrez Calcium binding region: SITE 1 (POTENTIA 83..94
Entrez Calcium binding region: SITE 2 (POTENTIA 113..124
Entrez Domain: ANCESTRAL CALCIUM SITE 3 (POTENT 148..158
Entrez Domain: ANCESTRAL CALCIUM SITE 4 (POTENT 177..190
PFAM efhand: EF hand 74..102
PFAM efhand: EF hand 104..132
PRODOM PD003609: CAN2(4) CANS(4) SORC(2) 27..107
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 109..160
PRODOM PD018845: SORC(2) GRAN(1) 162..197
PROSITE EF_HAND: EF-hand calcium-binding domain. 113..125
PROSITE EF_HAND: EF-hand calcium-binding domain. 83..95
__________________
Plus Strand HSPs:
Score = 145 (51.0 bits), Expect = 6.2e-09, P = 6.2e-09
Identities = 36/103 (34%), Positives = 55/103 (53%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G EF LW+ L WR F + DRSG +DP EL+ AL +G+ + + + +Y
Sbjct: 91 GFNEFKELWAVLNGWRQHFISFDSDRSGTVDPQELQKALTTMGFRLNPQTVNSIAKRYST 150
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYG---FSYT-FIR 450
SG+ + FD ++ C + ++ LTD F+ G FSY FI+
Sbjct: 151 -SGK---ITFDDYIACCVKLRALTDSFRRRDSAQQGMVNFSYDDFIQ 193
>gi|7289349|gb|AAF45393.1| (AE003000) CG17661 gene product [Drosophila
melanogaster]
Length = 219
Frame 1 hits (HSPs): __________________
__________________________________________________
Database sequence: | | | | | | 219
0 50 100 150 200
Plus Strand HSPs:
Score = 146 (51.4 bits), Expect = 1.0e-07, P = 1.0e-07
Identities = 29/78 (37%), Positives = 45/78 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
K+F ALW + W+ F + +D SG ID EL+ AL GY + ++ +LL K+ D
Sbjct: 69 KDFGALWKYVTDWQNCFRSFDRDNSGNIDKTELKTALTSFGYRLSDHLIDVLLRKF-DRF 127
Query: 328 GRRVELGFDSFVECGMII 381
GR L FD F++C +++
Sbjct: 128 GRGTIL-FDDFIQCCIVL 144
>gi|3212434|pdb|1AJ5|A Chain A, Calpain Domain Vi Apo >gi|3212435|pdb|1AJ5|B
Chain B, Calpain Domain Vi Apo
Length = 173
Frame 1 hits (HSPs): ______________________
__________________________________________________
Database sequence: | | | | | 173
0 50 100 150
Plus Strand HSPs:
Score = 130 (45.8 bits), Expect = 3.1e-07, P = 3.1e-07
Identities = 25/76 (32%), Positives = 43/76 (56%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+GI++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 65 GFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSD 124
Query: 322 GSGRRVELGFDSFVEC 369
+G + FD+F+ C
Sbjct: 125 ETGN---MDFDNFISC 137
>gi|3024652|sp|Q94743|SORC_SCHJA SORCIN >gi|1655733|gb|AAB17908.1| (U39069)
sorcin [Schistosoma japonicum]
Length = 171
Frame 1 hits (HSPs): _____________________________
Annotated Domains: _________________________________________________
__________________________________________________
Database sequence: | | | | | 171
0 50 100 150
__________________
Annotated Domains:
BLOCKS BL00018: EF-hand calcium-binding domain 53..65
Entrez Calcium binding region: POTENTIAL. 16..27
Entrez Calcium binding region: POTENTIAL. 53..64
Entrez Calcium binding region: POTENTIAL. 83..94
PFAM efhand: EF hand 7..35
PFAM efhand: EF hand 44..72
PFAM efhand: EF hand 74..102
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 6..65
PRODOM PD000012: CALM(76) PRVA(15) PRVB(14) 79..130
PRODOM PD048635: SORC_SCHJA 132..170
PROSITE EF_HAND: EF-hand calcium-binding domain. 16..28
PROSITE EF_HAND: EF-hand calcium-binding domain. 53..65
PROSITE EF_HAND: EF-hand calcium-binding domain. 83..95
PROSITE LEUCINE_ZIPPER: Leucine zipper pattern. 92..113
__________________
Plus Strand HSPs:
Score = 128 (45.1 bits), Expect = 5.3e-07, P = 5.3e-07
Identities = 30/96 (31%), Positives = 50/96 (52%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF L+ + W+ F RY +D SG ID E +AL GY + + L++ ++ G
Sbjct: 64 EFLGLFKYVQDWQTCFRRYDRDNSGSIDLNEFSNALISFGYHLSPQFVNLMMRRFDRNRG 123
Query: 331 RRVELGFDSFVECGMIIKGLTDKF-----KGEGYTVYGF 432
+ FD F+ + ++ LT +F +G G+TV+ F
Sbjct: 124 ---SIAFDDFIYACVCLQTLTREFSRYDCRGIGHTVFSF 159
>gi|5051921|gb|AAD38363.1| (AF139373) calpain small subunit [Mus musculus]
Length = 170
Frame 1 hits (HSPs): ______________________
__________________________________________________
Database sequence: | | | | | 170
0 50 100 150
Plus Strand HSPs:
Score = 124 (43.7 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 25/76 (32%), Positives = 42/76 (55%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ I++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 90 GFEEFKYLWNNIKKWQAIYKRFDTDRSGTIASHELPGAFEAAGFHLNEHLYSMIIRRYAD 149
Query: 322 GSGRRVELGFDSFVEC 369
SG + FD+F+ C
Sbjct: 150 ESGN---MDFDNFISC 162
>gi|10835527|pdb|1F4O|A Chain A, Crystal Structure Of Grancalcin With Bound
Calcium >gi|10835528|pdb|1F4O|B Chain B, Crystal Structure Of
Grancalcin With Bound Calcium >gi|10835529|pdb|1F4Q|A Chain A,
Crystal Structure Of Apo Grancalcin >gi|10835530|pdb|1F4Q|B Chain
B, Crystal Structure Of Apo Grancalcin
Length = 165
Frame 1 hits (HSPs): ______________________________
__________________________________________________
Database sequence: | | | | | 165
0 50 100 150
Plus Strand HSPs:
Score = 124 (43.7 bits), Expect = 1.5e-06, P = 1.5e-06
Identities = 33/102 (32%), Positives = 51/102 (50%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G F LW+ L W+ F +D SG ++ ELR A+ +GY + L ++ +Y
Sbjct: 58 GFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSK 117
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYGFSYTFI 447
+GR + FD +V C + ++ LTD FK + G S FI
Sbjct: 118 -NGR---IFFDDYVACCVKLRALTDFFKKRDHLQQG-SADFI 154
>gi|2493454|sp|Q64537|CANS_RAT CALCIUM-DEPENDENT PROTEASE, SMALL SUBUNIT
(CALPAIN REGULATORY SUBUNIT) (CALCIUM-ACTIVATED NEUTRAL PROTEINASE)
(CANP) >gi|1083614|pir||A55143 calpain (EC 3.4.22.17) light chain -
rat (fragment) >gi|9257038|pdb|1DF0|B Chain B, Crystal Structure Of
M-Calpain >gi|505658|gb|AAA64828.1| (U10861) calpain small subunit
[Rattus norvegicus]
Length = 184
Frame 1 hits (HSPs): ____________________
Annotated Domains: ________________________________________________
__________________________________________________
Database sequence: | | | | | 184
0 50 100 150
__________________
Annotated Domains:
Entrez Domain: CALCIUM-BINDING. 12..184
Entrez Calcium binding region: SITE 1 (POTENTIA 68..79
Entrez Calcium binding region: SITE 2 (POTENTIA 98..109
Entrez Domain: ANCESTRAL CALCIUM SITE 3 (POTENT 133..144
Entrez Domain: ANCESTRAL CALCIUM SITE 4 (POTENT 163..176
PFAM efhand: EF hand 59..87
PFAM efhand: EF hand 89..117
PFAM efhand: EF hand 154..184
PROSITE EF_HAND: EF-hand calcium-binding domain. 68..80
PROSITE EF_HAND: EF-hand calcium-binding domain. 98..110
__________________
Plus Strand HSPs:
Score = 130 (45.8 bits), Expect = 2.2e-06, P = 2.2e-06
Identities = 25/76 (32%), Positives = 43/76 (56%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+GI++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 76 GFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSD 135
Query: 322 GSGRRVELGFDSFVEC 369
+G + FD+F+ C
Sbjct: 136 ETGN---MDFDNFISC 148
>gi|3212511|pdb|1DVI|A Chain A, Calpain Domain Vi With Calcium Bound
>gi|3212512|pdb|1DVI|B Chain B, Calpain Domain Vi With Calcium
Bound
Length = 184
Frame 1 hits (HSPs): ____________________
__________________________________________________
Database sequence: | | | | | 184
0 50 100 150
Plus Strand HSPs:
Score = 130 (45.8 bits), Expect = 2.2e-06, P = 2.2e-06
Identities = 25/76 (32%), Positives = 43/76 (56%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+GI++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 76 GFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSD 135
Query: 322 GSGRRVELGFDSFVEC 369
+G + FD+F+ C
Sbjct: 136 ETGN---MDFDNFISC 148
>gi|1079058|pir||A55054 calpain (EC 3.4.22.17) large chain - fruit fly
(Drosophila melanogaster) >gi|562288|emb|CAA55297.1| (X78555)
calpain [Drosophila melanogaster]
Length = 805
Frame 1 hits (HSPs): ______
Annotated Domains: _________________ _________
__________________________________________________
Database sequence: | | | | | | | 805
0 150 300 450 600 750
__________________
Annotated Domains:
Entrez domain: calpain catalytic domain homolog 90..347
Entrez domain: calmodulin repeat homology #labe 676..708
Entrez domain: calmodulin repeat homology #labe 709..738
Entrez domain: calmodulin repeat homology #labe 741..773
Entrez domain: calmodulin repeat homology #labe 774..805
PROSITE EF_HAND: EF-hand calcium-binding domain. 689..701
PROSITE THIOL_PROTEASE_CYS: Eukaryotic thiol (cy 114..125
__________________
Plus Strand HSPs:
Score = 143 (50.3 bits), Expect = 6.4e-06, P = 6.4e-06
Identities = 31/88 (35%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF L S + W+ IF+ Y + +G++ +LR+AL GY + VL +L +YG
Sbjct: 697 GFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGS 756
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G+ + FD F+ C + IK D FK
Sbjct: 757 RDGK---IAFDDFIMCAVKIKTYIDIFK 781
>gi|1705574|sp|Q11002|CAN_DROME CALPAIN (CALCIUM-ACTIVATED NEUTRAL PROTEINASE)
(CANP) >gi|562289|emb|CAA55298.1| (X78555) calpain [Drosophila
melanogaster]
Length = 828
Frame 1 hits (HSPs): ______
Annotated Domains: __________________________________________________
__________________________________________________
Database sequence: | | | | | | | 828
0 150 300 450 600 750
__________________
Annotated Domains:
DOMO DM01305: CALPAINCATALYTICDOMAIN 44..553
DOMO DM01246: CALPAINCATALYTICDOMAIN 555..610
DOMO DM00011: CALMODULINREPEAT 687..724
DOMO DM01221: CALPAINCATALYTICDOMAIN 726..826
Entrez Domain: I, REGULATION OF PROTEASE ACTIVI 1..112
Entrez Domain: II, THIOL PROTEASE. 113..394
Entrez Domain: III, CONTAINS TWO REPEATS. 395..562
Entrez Domain: IV, CALCIUM-BINDING. 563..828
Entrez active site: BY SIMILARITY. 143
Entrez active site: BY SIMILARITY. 299
Entrez active site: BY SIMILARITY. 327
Entrez Calcium binding region: SITE 1 (PROBABLE 712..724
Entrez Calcium binding region: SITE 2 (PROBABLE 742..753
Entrez Domain: ANCESTRAL CALCIUM SITE 3 (POTENT 777..789
Entrez Domain: ANCESTRAL CALCIUM SITE 4 (POTENT 807..819
Entrez Splicing variant: ENDDH -> RTSRQ (IN SHO 554..558
Entrez Splicing variant: MISSING (IN SHORT FORM 559..828
PFAM Peptidase_C2: Calpain family cysteine pr 88..387
PFAM Calpain_III: Calpain large subunit, doma 402..553
PFAM efhand: EF hand 703..731
PFAM efhand: EF hand 733..761
PRINTS CALPAIN1: Calpain motif I - 2 73..96
PRINTS CALPAIN2: Calpain motif II - 2 113..135
PRINTS CALPAIN3: Calpain motif III - 2 137..153
PRINTS CALPAIN4: Calpain motif IV - 2 173..198
PRINTS CALPAIN5: Calpain motif V - 2 203..226
PRINTS CALPAIN6: Calpain motif VI - 2 228..255
PRINTS CALPAIN7: Calpain motif VII - 2 363..384
PRINTS CALPAIN8: Calpain motif VIII - 2 417..434
PRINTS CALPAIN9: Calpain motif IX - 2 519..547
PRODOM PD112555: CAN_DROME 1..71
PRODOM PD002154: CAN2(4) CAN3(4) CAN(2) 73..171
PRODOM PD001545: CAN3(8) CAN2(4) CAN(2) 175..385
PRODOM PD001874: CAN2(8) CAN3(5) CAN(2) 388..552
PRODOM PD112565: CAN_DROME 554..580
PRODOM PD003609: CAN2(4) CANS(4) SORC(2) 582..610
PRODOM PD197268: CAN_DROME 612..694
PRODOM PD003609: CAN2(4) CANS(4) SORC(2) 696..723
PRODOM PD002827: CAN2(4) CANS(4) CAN3(3) 727..789
PRODOM PD054717: CAN(1) O96454(1) 791..827
PROSITE EF_HAND: EF-hand calcium-binding domain. 712..724
PROSITE THIOL_PROTEASE_CYS: Eukaryotic thiol (cy 137..148
__________________
Plus Strand HSPs:
Score = 143 (50.3 bits), Expect = 6.7e-06, P = 6.7e-06
Identities = 31/88 (35%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF L S + W+ IF+ Y + +G++ +LR+AL GY + VL +L +YG
Sbjct: 720 GFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGS 779
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G+ + FD F+ C + IK D FK
Sbjct: 780 RDGK---IAFDDFIMCAVKIKTYIDIFK 804
>gi|600420|emb|CAA86993.1| (Z46891) Calpain [Drosophila melanogaster]
Length = 828
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 828
0 150 300 450 600 750
Plus Strand HSPs:
Score = 143 (50.3 bits), Expect = 6.7e-06, P = 6.7e-06
Identities = 31/88 (35%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF L S + W+ IF+ Y + +G++ +LR+AL GY + VL +L +YG
Sbjct: 720 GFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGS 779
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G+ + FD F+ C + IK D FK
Sbjct: 780 RDGK---IAFDDFIMCAVKIKTYIDIFK 804
>gi|7302479|gb|AAF57563.1| (AE003796) CalpA gene product [Drosophila
melanogaster]
Length = 828
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 828
0 150 300 450 600 750
Plus Strand HSPs:
Score = 143 (50.3 bits), Expect = 6.7e-06, P = 6.7e-06
Identities = 31/88 (35%), Positives = 48/88 (54%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF L S + W+ IF+ Y + +G++ +LR+AL GY + VL +L +YG
Sbjct: 720 GFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGS 779
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G+ + FD F+ C + IK D FK
Sbjct: 780 RDGK---IAFDDFIMCAVKIKTYIDIFK 804
>gi|2506056|dbj|BAA22638.1| (AB007824) calpain small subunit [Gallus gallus]
Length = 214
Frame 1 hits (HSPs): _____________________
__________________________________________________
Database sequence: | | | | | | 214
0 50 100 150 200
Plus Strand HSPs:
Score = 130 (45.8 bits), Expect = 1.2e-05, P = 1.2e-05
Identities = 29/88 (32%), Positives = 47/88 (53%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ +++R+ DRSG I EL A G+ +P + +L +YGD
Sbjct: 106 GFEEFKYLWNNIKKWQLVYKRFDTDRSGTIGVQELPGAFEAAGFRLPPELWGVLGRRYGD 165
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G L FD+F+ C + + + FK
Sbjct: 166 EGGN---LDFDNFISCLVRLDAMFRAFK 190
>gi|6980466|pdb|1DKV|B Chain B, The Crystal Structure Of Calcium-Free Human
M-Calpain
Length = 184
Frame 1 hits (HSPs): ________________________
__________________________________________________
Database sequence: | | | | | 184
0 50 100 150
Plus Strand HSPs:
Score = 124 (43.7 bits), Expect = 2.2e-05, P = 2.2e-05
Identities = 26/88 (29%), Positives = 47/88 (53%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ I++++ DRSG I EL A G+ + + +++ +Y D
Sbjct: 76 GFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSD 135
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
SG + FD+F+ C + + + FK
Sbjct: 136 ESGN---MDFDNFISCLVRLDAMFRAFK 160
>gi|1794205|gb|AAC53002.1| (U53859) calpain small subunit [Rattus norvegicus]
Length = 266
Frame 1 hits (HSPs): _______________
__________________________________________________
Database sequence: | | | | | | | 266
0 50 100 150 200 250
Plus Strand HSPs:
Score = 130 (45.8 bits), Expect = 2.8e-05, P = 2.8e-05
Identities = 25/76 (32%), Positives = 43/76 (56%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+GI++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 158 GFEEFKYLWNNIKKWQGIYKRFDTDRSGTIGSNELPGAFEAAGFHLNQHIYSMIIRRYSD 217
Query: 322 GSGRRVELGFDSFVEC 369
+G + FD+F+ C
Sbjct: 218 ETGN---MDFDNFISC 230
>gi|2358260|gb|AAB69114.1| (U89513) calpain large subunit [Mus musculus]
Length = 688
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | 688
0 150 300 450 600
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 3.3e-05, P = 3.3e-05
Identities = 26/74 (35%), Positives = 42/74 (56%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF W L HW +F ++ D+SG + ELR AL G+ + G +LQL++ +Y D
Sbjct: 582 EEFRVFWDKLKHWMDLFLQFDVDKSGTMSSYELRTALKAAGFQLGGHLLQLIVLRYADED 641
Query: 328 GRRVELGFDSFVEC 369
++L FD ++ C
Sbjct: 642 ---LQLDFDDYLNC 652
>gi|3820523|gb|AAD04331.1| (AF062404) calpain [Drosophila melanogaster]
Length = 791
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 791
0 150 300 450 600 750
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 4.0e-05, P = 4.0e-05
Identities = 29/88 (32%), Positives = 46/88 (52%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF AL + + WR +F+ Y R+G ID LR AL GY + +L L +YG
Sbjct: 683 GFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGS 742
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G ++ FD F+ C + ++ + F+
Sbjct: 743 REG---QIPFDDFLMCAIKVRTFIEMFR 767
>gi|7294858|gb|AAF50189.1| (AE003550) CalpB gene product [Drosophila
melanogaster]
Length = 791
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 791
0 150 300 450 600 750
Plus Strand HSPs:
Score = 136 (47.9 bits), Expect = 4.0e-05, P = 4.0e-05
Identities = 29/88 (32%), Positives = 46/88 (52%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF AL + + WR +F+ Y R+G ID LR AL GY + +L L +YG
Sbjct: 683 GFEEFEALLTDIAKWRAVFKLYDTRRTGSIDGFHLRGALNSAGYHLNNRLLNALAHRYGS 742
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G ++ FD F+ C + ++ + F+
Sbjct: 743 REG---QIPFDDFLMCAIKVRTFIEMFR 767
>gi|3319068|pdb|1ALV|A Chain A, Calcium Bound Domain Vi Of Porcine Calpain
>gi|3319069|pdb|1ALV|B Chain B, Calcium Bound Domain Vi Of Porcine
Calpain >gi|3319037|pdb|1ALW|A Chain A, Inhibitor And Calcium Bound
Domain Vi Of Porcine Calpain >gi|3319038|pdb|1ALW|B Chain B,
Inhibitor And Calcium Bound Domain Vi Of Porcine Calpain
Length = 173
Frame 1 hits (HSPs): _________________________
__________________________________________________
Database sequence: | | | | | 173
0 50 100 150
Plus Strand HSPs:
Score = 120 (42.2 bits), Expect = 4.2e-05, P = 4.2e-05
Identities = 25/88 (28%), Positives = 46/88 (52%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ I++++ DRSG I EL A G+ + + +++ +Y D
Sbjct: 65 GFEEFKYLWNNIKKWQAIYKQFDVDRSGTIGSSELPGAFEAAGFHLNEHLYSMIIRRYSD 124
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
G + FD+F+ C + + + FK
Sbjct: 125 EGGN---MDFDNFISCLVRLDAMFRAFK 149
>gi|6753256 ref|NP_033925.1| calpain 4 [Mus musculus]
>gi|3511116|gb|AAC97194.1| (AF058298) calpain small subunit [Mus
musculus]
Length = 269
Frame 1 hits (HSPs): _________________
__________________________________________________
Database sequence: | | | | | | | 269
0 50 100 150 200 250
Plus Strand HSPs:
Score = 128 (45.1 bits), Expect = 5.0e-05, P = 5.0e-05
Identities = 27/88 (30%), Positives = 47/88 (53%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ I++R+ DRSG I EL A G+ + + +++ +Y D
Sbjct: 161 GFEEFKYLWNNIKKWQAIYKRFDTDRSGTIGSHELPGAFEAAGFHLNEHLYSMIIRRYAD 220
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
SG + FD+F+ C + + + FK
Sbjct: 221 ESGN---MDFDNFISCLVRLDAMFRAFK 245
>gi|11422561 ref|XP_001446.1| GC36 protein [Homo sapiens]
Length = 571
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | 571
0 150 300 450
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 9.6e-05, P = 9.6e-05
Identities = 26/73 (35%), Positives = 39/73 (53%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF W L W +F R+ D+SG + ELR AL G+ + +LQL++ +Y D
Sbjct: 466 EFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD--- 522
Query: 331 RRVELGFDSFVEC 369
++L FD F+ C
Sbjct: 523 EELQLDFDDFLNC 535
>gi|4960061|gb|AAD34601.1| (AF148956) lens-specific calpain Lp82 [Oryctolagus
cuniculus]
Length = 709
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 709
0 150 300 450 600
Plus Strand HSPs:
Score = 132 (46.5 bits), Expect = 0.00010, P = 0.00010
Identities = 26/85 (30%), Positives = 48/85 (56%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW + W+ IF+RY D SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 603 QEFHHLWKKIKAWQKIFKRYDTDHSGTINSYEMRNAVKDAGFHLNSQLYDIITMRYAD-- 660
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 661 -KHMNIDFDSFICCFIRLEGMFRAF 684
>gi|11422559 ref|XP_001445.1| calpain 9 (nCL-4) [Homo sapiens]
Length = 597
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | 597
0 150 300 450
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 0.00010, P = 0.00010
Identities = 26/73 (35%), Positives = 39/73 (53%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF W L W +F R+ D+SG + ELR AL G+ + +LQL++ +Y D
Sbjct: 492 EFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD--- 548
Query: 331 RRVELGFDSFVEC 369
++L FD F+ C
Sbjct: 549 EELQLDFDDFLNC 561
>gi|7705383 ref|NP_057536.1| GC36 protein [Homo sapiens]
>gi|7023943|dbj|BAA92137.1| (AB038463) down-regulated in gastric
cancer [Homo sapiens]
Length = 664
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | 664
0 150 300 450 600
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 0.00012, P = 0.00012
Identities = 26/73 (35%), Positives = 39/73 (53%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF W L W +F R+ D+SG + ELR AL G+ + +LQL++ +Y D
Sbjct: 559 EFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD--- 615
Query: 331 RRVELGFDSFVEC 369
++L FD F+ C
Sbjct: 616 EELQLDFDDFLNC 628
>gi|5729758 ref|NP_006606.1| calpain 9 (nCL-4) [Homo sapiens]
>gi|2502077|gb|AAB80762.1| (AF022799) digestive tract-specific
calpain; calcium-dependent cysteine proteinase [Homo sapiens]
Length = 690
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | 690
0 150 300 450 600
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 0.00012, P = 0.00012
Identities = 26/73 (35%), Positives = 39/73 (53%), Frame = +1
Query: 151 EFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGSG 330
EF W L W +F R+ D+SG + ELR AL G+ + +LQL++ +Y D
Sbjct: 585 EFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSHLLQLIVLRYAD--- 641
Query: 331 RRVELGFDSFVEC 369
++L FD F+ C
Sbjct: 642 EELQLDFDDFLNC 654
>gi|6912388 ref|NP_036330.1| grancalcin [Homo sapiens]
>gi|1170014|sp|P28676|GRAN_HUMAN GRANCALCIN >gi|284135|pir||A42578
EF-hand calcium-binding protein grancalcin - human
>gi|183031|gb|AAA58498.1| (M81637) grancalcin [Homo sapiens]
Length = 217
Frame 1 hits (HSPs): _______________________
__________________________________________________
Database sequence: | | | | | | 217
0 50 100 150 200
Plus Strand HSPs:
Score = 122 (42.9 bits), Expect = 0.00013, P = 0.00013
Identities = 32/102 (31%), Positives = 51/102 (50%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G F LW+ L W+ F +D SG ++ ELR A+ +GY + L ++ +Y
Sbjct: 110 GFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSK 169
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYGFSYTFI 447
+GR + FD +V C + ++ LTD F+ + G S FI
Sbjct: 170 -NGR---IFFDDYVACCVKLRALTDFFRKRDHLQQG-SANFI 206
>gi|4960059|gb|AAD34600.1| (AF148955) lens-specific calpain Lp82 [Sus scrofa]
Length = 709
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 709
0 150 300 450 600
Plus Strand HSPs:
Score = 131 (46.1 bits), Expect = 0.00013, P = 0.00013
Identities = 25/85 (29%), Positives = 48/85 (56%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 603 QEFHHLWKKIKSWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 660
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 661 -KYMNIDFDSFIRCFVRLEGMFRAF 684
>gi|4502565 ref|NP_001740.1| calpain 4 small subunit; calpain, small
polypeptide; calcium-dependent protease, small subunit;
calcium-activated neutral proteinase [Homo sapiens] >gi|11426701
ref|XP_009338.1| calpain 4 small subunit [Homo sapiens]
>gi|115612|sp|P04632|CANS_HUMAN CALCIUM-DEPENDENT PROTEASE, SMALL
SUBUNIT (CALPAIN REGULATORY SUBUNIT) (CALCIUM-ACTIVATED NEUTRAL
PROTEINASE) (CANP) >gi|67665|pir||CIHUL calpain (EC 3.4.22.17)
small chain - human >gi|35328|emb|CAA27726.1| (X04106) protease
small subunit (aa 1-268) [Homo sapiens] >gi|179909|gb|AAA35646.1|
(M31511) neutral protease alpha subunit [Homo sapiens]
>gi|1905903|gb|AAB51183.1| (AD001527) calcium-dependent protease,
small (regulatory) subunit (calpain) (calcium-activated neutral
proteinase) (CANP) [Homo sapiens] >gi|2443873|gb|AAB81546.1|
(AC002984) CANS_Human [Homo sapiens]
>gi|12653629|gb|AAH00592.1|AAH00592 (BC000592) calpain 4, small
subunit (30K) [Homo sapiens]
Length = 268
Frame 1 hits (HSPs): _________________
__________________________________________________
Database sequence: | | | | | | | 268
0 50 100 150 200 250
Plus Strand HSPs:
Score = 124 (43.7 bits), Expect = 0.00015, P = 0.00015
Identities = 26/88 (29%), Positives = 47/88 (53%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G +EF LW+ + W+ I++++ DRSG I EL A G+ + + +++ +Y D
Sbjct: 160 GFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSD 219
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFK 405
SG + FD+F+ C + + + FK
Sbjct: 220 ESGN---MDFDNFISCLVRLDAMFRAFK 244
>gi|11428473 ref|XP_002208.1| grancalcin [Homo sapiens]
Length = 256
Frame 1 hits (HSPs): ____________________
__________________________________________________
Database sequence: | | | | | | | 256
0 50 100 150 200 250
Plus Strand HSPs:
Score = 122 (42.9 bits), Expect = 0.00023, P = 0.00023
Identities = 32/102 (31%), Positives = 51/102 (50%), Frame = +1
Query: 142 GPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGD 321
G F LW+ L W+ F +D SG ++ ELR A+ +GY + L ++ +Y
Sbjct: 149 GFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSK 208
Query: 322 GSGRRVELGFDSFVECGMIIKGLTDKFKGEGYTVYGFSYTFI 447
+GR + FD +V C + ++ LTD F+ + G S FI
Sbjct: 209 -NGR---IFFDDYVACCVKLRALTDFFRKRDHLQQG-SANFI 245
>gi|7684607|gb|AAD28254.3|AF127765_1 (AF127765) calpain 3; calcium activated
neutral protease; CAPN3; CL1 [Homo sapiens]
Length = 729
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 729
0 150 300 450 600
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00023, P = 0.00023
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 623 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 680
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 681 -KHMNIDFDSFICCFVRLEGMFRAF 704
>gi|87013|pir||A34488 calpain (EC 3.4.22.17) heavy chain - human (fragment)
Length = 778
Frame 1 hits (HSPs): ______
Annotated Domains: ____________________ ____________
__________________________________________________
Database sequence: | | | | | | | 778
0 150 300 450 600 750
__________________
Annotated Domains:
Entrez domain: calpain catalytic domain homolog 56..357
Entrez domain: calmodulin repeat homology #labe 606..637
Entrez domain: calmodulin repeat homology #labe 649..681
Entrez domain: calmodulin repeat homology #labe 682..711
Entrez domain: calmodulin repeat homology #labe 714..746
Entrez domain: calmodulin repeat homology #labe 747..778
Entrez active site: Cys, His, Asn 86
Entrez active site: Cys, His, Asn 291
Entrez active site: Cys, His, Asn 315
PROSITE EF_HAND: EF-hand calcium-binding domain. 662..674
PROSITE EF_HAND: EF-hand calcium-binding domain. 692..704
PROSITE THIOL_PROTEASE_CYS: Eukaryotic thiol (cy 80..91
__________________
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00025, P = 0.00025
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 672 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 729
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 730 -KHMNIDFDSFICCFVRLEGMFRAF 753
>gi|4704752|gb|AAD28253.1|AF127764_1 (AF127764) calpain 3; calcium activated
neutral protease; CAPN3; CL1 [Homo sapiens]
Length = 815
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 815
0 150 300 450 600 750
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00026, P = 0.00026
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 709 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 766
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 767 -KHMNIDFDSFICCFVRLEGMFRAF 790
>gi|11037484|gb|AAG27599.1|AF277376_1 (AF277376) calpain 3 [Macaca
fascicularis]
Length = 815
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 815
0 150 300 450 600 750
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00026, P = 0.00026
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 709 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 766
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 767 -KHMNIDFDSFICCFVRLEGMFRAF 790
>gi|4557405 ref|NP_000061.1| calpain, large polypeptide L3; p94 [Homo sapiens]
>gi|1345664|sp|P20807|CAN3_HUMAN CALPAIN 3 LARGE SUBUNIT (CALPAIN
L3) (CALPAIN P94, LARGE [CATALYTIC] SUBUNIT) (CALCIUM-ACTIVATED
NEUTRAL PROTEINASE 3) (CANP 3) (MUSCLE-SPECIFIC CALCIUM-ACTIVATED
NEUTRAL PROTEASE 3 LARGE SUBUNIT) >gi|1082271|pir||A56218 calpain
(EC 3.4.22.17) 3 large chain - human >gi|7428243|pir||CIHUH3
calpain (EC 3.4.22.17) large chain 3 - human
>gi|791040|emb|CAA59403.1| (X85030) calpain [Homo sapiens]
Length = 821
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 821
0 150 300 450 600 750
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00027, P = 0.00027
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 715 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 772
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 773 -KHMNIDFDSFICCFVRLEGMFRAF 796
>gi|12738707 ref|XP_007541.2| calpain, large polypeptide L3 [Homo sapiens]
Length = 845
Frame 1 hits (HSPs): ______
__________________________________________________
Database sequence: | | | | | | | 845
0 150 300 450 600 750
Plus Strand HSPs:
Score = 129 (45.4 bits), Expect = 0.00028, P = 0.00028
Identities = 25/85 (29%), Positives = 49/85 (57%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW+ + W+ IF+ Y D+SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 739 QEFHHLWNKIKAWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYAD-- 796
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 797 -KHMNIDFDSFICCFVRLEGMFRAF 820
>gi|1628587|gb|AAB17381.1| (U71320) calpain II [Sus scrofa]
Length = 203
Frame 1 hits (HSPs): _________________________
__________________________________________________
Database sequence: | | | | || 203
0 50 100 150 200
Plus Strand HSPs:
Score = 118 (41.5 bits), Expect = 0.00030, P = 0.00030
Identities = 28/95 (29%), Positives = 53/95 (55%), Frame = +1
Query: 88 GWSVPLC*FQLPV----ACAFTGPKEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDA 255
G+S+ C + + A G KEF LW+ + ++ I+ DRSG ++ E+R A
Sbjct: 74 GFSIETCKIMVDMLDSDGSAKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKA 133
Query: 256 LYGIGYAVPGSVLQLLLSKYGDGSGRRVELGFDSFVEC 369
L G+ +P + Q++++++ D ++ + FD+FV C
Sbjct: 134 LEEAGFKLPCQLHQVIVARFADD---QLIIDFDNFVRC 168
>gi|2584822|gb|AAC04848.1| (U96367) calpain Lp82 [Rattus norvegicus]
Length = 709
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 709
0 150 300 450 600
Plus Strand HSPs:
Score = 127 (44.7 bits), Expect = 0.00037, P = 0.00037
Identities = 25/85 (29%), Positives = 47/85 (55%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW + W+ IF+ Y D SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 603 QEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYAD-- 660
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 661 -KHMNIDFDSFICCFVRLEGMFRAF 684
>gi|3661585|gb|AAC61764.1| (AF091998) calpain Lp82 [Mus musculus]
Length = 709
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 709
0 150 300 450 600
Plus Strand HSPs:
Score = 127 (44.7 bits), Expect = 0.00037, P = 0.00037
Identities = 25/85 (29%), Positives = 47/85 (55%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW + W+ IF+ Y D SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 603 QEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYAD-- 660
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 661 -KHMNIDFDSFICCFVRLEGMFRAF 684
>gi|7674485|gb|AAD28255.2|AF127766_1 (AF127766) calpain 3; calcium activated
neutral protease [Mus musculus]
Length = 729
Frame 1 hits (HSPs): _______
__________________________________________________
Database sequence: | | | | | | 729
0 150 300 450 600
Plus Strand HSPs:
Score = 127 (44.7 bits), Expect = 0.00038, P = 0.00038
Identities = 25/85 (29%), Positives = 47/85 (55%), Frame = +1
Query: 148 KEFAALWSCLGHWRGIFERYXKDRSGKIDPLELRDALYGIGYAVPGSVLQLLLSKYGDGS 327
+EF LW + W+ IF+ Y D SG I+ E+R+A+ G+ + + ++ +Y D
Sbjct: 623 QEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYAD-- 680
Query: 328 GRRVELGFDSFVECGMIIKGLTDKF 402
+ + + FDSF+ C + ++G+ F
Sbjct: 681 -KHMNIDFDSFICCFVRLEGMFRAF 704
WARNING: HSPs involving 80 database sequences were not reported due to the
limiting value of parameter B = 50.
Parameters:
filter=none
matrix=BLOSUM62
V=50
B=50
E=10
gi
H=1
sort_by_pvalue
echofilter
ctxfactor=5.96
Query ----- As Used ----- ----- Computed ----
Frame MatID Matrix name Lambda K H Lambda K H
Std. 0 BLOSUM62 0.318 0.135 0.401
+3 0 BLOSUM62 0.318 0.135 0.401 0.361 0.161 0.657
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+2 0 BLOSUM62 0.318 0.135 0.401 0.346 0.154 0.498
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
+1 0 BLOSUM62 0.318 0.135 0.401 0.347 0.159 0.558
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-1 0 BLOSUM62 0.318 0.135 0.401 0.345 0.149 0.496
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-2 0 BLOSUM62 0.318 0.135 0.401 0.355 0.158 0.564
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
-3 0 BLOSUM62 0.318 0.135 0.401 0.360 0.161 0.562
Q=9,R=2 0.244 0.0300 0.180 n/a n/a n/a
Query
Frame MatID Length Eff.Length E S W T X E2 S2
+3 0 272 258 10. 78 3 12 22 0.091 36
32 0.11 39
+2 0 272 260 10. 78 3 12 22 0.092 36
32 0.11 39
+1 0 273 261 10. 78 3 12 22 0.092 36
32 0.11 39
-1 0 273 260 10. 78 3 12 22 0.092 36
32 0.11 39
-2 0 272 257 10. 78 3 12 22 0.12 35
32 0.11 39
-3 0 272 259 10. 78 3 12 22 0.091 36
32 0.11 39
Statistics:
Database: /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
Title: nr
Release date: unknown
Posted date: 4:06 PM CST Feb 28, 2001
Format: BLAST
# of letters in database: 197,782,623
# of sequences in database: 625,274
# of database sequences satisfying E: 130
No. of states in DFA: 599 (59 KB)
Total size of DFA: 262 KB (320 KB)
Time to generate neighborhood: 0.01u 0.00s 0.01t Elapsed: 00:00:00
No. of threads or processors used: 6
Search cpu time: 243.94u 0.88s 244.82t Elapsed: 00:01:20
Total cpu time: 244.00u 0.91s 244.91t Elapsed: 00:01:20
Start: Mon Oct 1 17:02:13 2001 End: Mon Oct 1 17:03:33 2001
WARNINGS ISSUED: 2
Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000