Fukasawa M, Okumura A, Shinomiya N, Rokutanda M, Sakakibara R; International Conference on AIDS.
Int Conf AIDS. 1998; 12: 555 (abstract no. 32175).
National Defense Medical College, Saitama, Japan.
OBJECTIVE: To assess the influence of HIV infection on the glycolysis, especially on the expression of a key regulatory enzyme of glycolysis in host cells. METHODS: The cDNA encoding an novel isozyme of human fructose-6-phosphate, 2-kinase/fructose-2,6-bisphosphatase kinase/Fru-2,6BPase) which is remarkably expressed in placenta, primary lymphocytes and monocytes/macrophages and their cell lines was molecularly cloned and designated as HP2K. We designed a specific PCR-primer set (HP-amp) for this isozyme, and then subjected the HIV- infected and uninfected cells to the semi-quantitative RT-PCR assay by using HP-amp and GAPDH primers as the internal control. The recombinant HIV carrying GFP instead of nef was used for monitoring and estimation of infectivity. RESULTS: The designed primer set (HP-amp) could specifically amplify HP2K mRNA. The semi-quantitative RT-PCR assay indicated that the amount of HP2K mRNA in HIV-infected T-cell lines (H9 and SupT1) decreased to less than 70% of that in uninfected cells, although the splicing pattern of HP2K was not affected by HIV infection. CONCLUSION: The semi-quantitative RT-PCR assay for human placental Fru-6-P,2-kinase/Fru-2,6 BPase suggests one of possibility that HIV infection disorders the glucose metabolism via repressive effect of transcription of the key enzyme for glycolysis.
Publication Types:
Keywords:
- DNA, Complementary
- Fructose
- Fructose-Bisphosphatase
- Fructosephosphates
- Glycolysis
- Humans
- Isoenzymes
- Phosphotransferases
- Placenta
- RNA, Messenger
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic
- fructose-6-phosphate
- genetics
Other ID:
UI: 102229690
From Meeting Abstracts