Sinet M, Levacher-Clergeot M, Ricatte L, Desforges B, Chau F, Pocidalo JJ; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: A69 (abstract no. PoA 2399).
INSERM U13, Hopital Claude Bernard, Paris, France.
OBJECTIVE: Mice infected with the Duplan strain of Murine Leukemia Virus (Dup MuLV) develop a well characterized lymphoproliferative, immunodeficiency disease (MAIDS) useful for the evaluation of potential antiviral agents and immunomodulators. To provide an additional marker for the characterization of disease progression, we studied the predictive value of alterations in blood lymphocyte populations on the outcome of disease in MAIDS. METHODS: Two groups of C57BI/6 mice were inoculated with Dup MuLV (SC1/Dup MuLV confluent fibroblasts supernatant, or spleen extract from one infected mouse). A third group of non-infected mice was taken as control. A 10 weeks follow-up of lymphocyte subsets in blood was performed using flow microfluorometric (FMF) analysis and anti-CD4, anti-CD8, anti-Thy 1,2, and anti-Ly5 monoclonal antibodies (mAbs). At week 10, mice were sacrificed and the examination of spleen and lymph nodes was also performed as well as a complementary phenotypic analysis (anti Ly-6c in combination with anti-CD4 and CD8, and anti-Mac-1 mAbs). RESULTS: A striking fall of the percentage of Thy 1,2+ blood lymphocytes (p less than 0.001) occurred in diseased mice, selectively due to a CD8+ cell decrease whereas the percentage of CD4+ cells remained unchanged (except a transient fall on weeks 6 to 9). At week 10, the residual population of CD8+ cells was primarily of Ly6c+ phenotype. Blood Ly5+ cell percentage transiently increased from week 2 to week 8 after inoculation. This population was also increased in spleen and lymph nodes. Ten weeks after inoculation, the percentage of spleen and lymph node macrophages was significantly increased (p less than 0.01). Finally, for each of the evaluated lymphocyte subsets, the phenotypic modifications observed in blood at the time of sacrifice were closely related to those observed in spleen and lymph nodes. CONCLUSION: These results suggest that phenotypic alterations of T cell subsets in blood are, as in human, a good biological marker for the follow-up of disease evolution and could be a useful endpoint to evaluate antiretroviral therapy in murine AIDS.
Publication Types:
Keywords:
- Animals
- CD4-Positive T-Lymphocytes
- CD8-Positive T-Lymphocytes
- Humans
- Immunologic Deficiency Syndromes
- Leukemia Virus, Murine
- Lymph Nodes
- Lymphocyte Count
- Lymphocyte Subsets
- Lymphocytes
- Mice
- Mice, Inbred C57BL
- Muridae
- Murine Acquired Immunodeficiency Syndrome
- Sjogren's Syndrome
- Spleen
- T-Lymphocytes
- blood
Other ID:
UI: 102198422
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