Managlia EZ, Steffens CM, Landay A, Al-Harthi L; Conference on Retroviruses and Opportunistic Infections.

9th Conf Retrovir Oppor Infect Feb 24 28 2002 Wash State Conv Trade Cent Seattle Wash Conf Retrovir Oppor Infect 9th 2002 Seattle Wash. 2002 Feb 24-28; 9: abstract no. 273-T.

Rush-Presbyterian St. Luke's Med. Ctr., Rush Univ., Chicago, IL

BACKGROUND: The predominant cellular targets for HIV productive infection are primed/memory CD4+ T cells. Although HIV gag/pol DNA can be detected in naive T cells, whether naive T cells can be productively infected by HIV is still questionable. Given that IL-7 is a prospective therapeutic immunomodulator for the treatment of HIV, we evaluated the effect of IL-7 on promoting naive T cell-infection of HIV.METHODS: Naive T cells (CD45RA+CD45RO-) were isolated by 2 rounds of CD45RO depletion and infected with laboratory-adapted (IIIB), M-tropic (Bal), and primary (30256,302073,302142,302144) isolates of HIV. IL-7 was added to the cultures either pre- or post-HIV infection. HIV-1 gag/pol DNA content was evaluated by real-time PCR and p24 measured using a standard ELISA.RESULTS: We demonstrate that although untreated or IL-2-treated naive T cells are not productively infected by HIV, IL-7 pre-treatment mediated the productive infection of laboratory-adapted, M-tropic, and primary isolates of HIV as determined by p24 core antigen production.This up-regulation was between 8- and 58-fold, depending on the HIV isolate used. IL-7 pre-treatment of naive T cells also potently up-regulated surface expression of CXCR4 but not CCR5 and mediated the expansion of naive T cells without the acquestion of the primed "CD45RO" phenotype in all donors.CONCLUSIONS: These data indicates that IL-7 auguments naive T-cell susepetibility to HIV and that under the appropriate envirnmental milieu, naive T cells may be a source of HIV productive infection. Our data also provide caution for the use of IL-7 as an immune modulator for HIV.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Vaccines
• Acquired Immunodeficiency Syndrome
• Antigens, CD4
• Antigens, CD45
• Genes, gag
• Genes, pol
• HIV Infections
• HIV Seropositivity
• HIV-1
• Interleukin-7
• Longitudinal Studies
• Receptors, CCR5
• Receptors, CXCR4
• T-Lymphocytes
• genetics
• immunology

Other ID:

• GWAIDS0023929

UI: 102263553

From Meeting Abstracts