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Source:
TEXAS A&M UNIV submitted to |
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A DEVELOPMENTAL MODEL OF MELANOMA
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PROJECT DIRECTOR: Amoss, M. S.
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PERFORMING ORGANIZATION
VETERINARY PHYSIOLOGY & PHARMACOLOGY
TEXAS A&M UNIV
COLLEGE STATION,TX 77843 |
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NON TECHNICAL SUMMARY:
The incidence of melanoma is rising rapidly, especially in the southwest. This research addresses the genetic parameters leading to the expression of melanoma and the physiological parameters producing spontaneous regression. The purpose of this research is to identify the genes responsible for the expression of melanoma, the specific role the genes play and the immunological mechanisms responsible for the spontaneous regression. With this information, final treatments will be developed.
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OBJECTIVES:
The overall objectives are to determine the cellular and molecular signals that init1ate tumorigenesis and regression in a swine model of melanoma. The projects are 1) to map and determine the genes responsible for expression of the tumor: 2) to determine the relative contribution of cytokines, macrophages and lymphocytes to regression: 3) to determine the roles of apoptosis and lack of telomerase to regression.
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APPROACH:
A herd of Sinclair swine will be maintained from which tumor tissue and white blood cells will be collected for use in various in vitro experiments. DNA will be used to map genes responsible for malanoma, once genes are identified and located, the role of that gene in causing the tumor will be investigated. As mechanisms are identified from the in vitro experiments, in vivo perturbations will be initiated to confirm and extend our observations.
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CRIS NUMBER: 0190420
SUBFILE: CRIS
PROJECT NUMBER: TEX08648
SPONSOR AGENCY: SAES
PROJECT TYPE: STATE
PROJECT STATUS: TERMINATED
MULTI-STATE PROJECT NUMBER: (N/A)
START DATE: Sep 11, 2001
TERMINATION DATE: Sep 10, 2006
GRANT PROGRAM: (N/A)
GRANT PROGRAM AREA: (N/A)
CLASSIFICATION
311 | 3510 | 1000 | 4.2 | 25% |
311 | 3510 | 1020 | 4.2 | 50% |
311 | 3510 | 1060 | 4.2 | 25% |
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CLASSIFICATION HEADINGS
KA311 - Animal Diseases S3510 - Swine, live animal F1020 - Physiology F1000 - Biochemistry and biophysics F1060 - Biology (whole systems) G4.2 - Reduce Number and Severity of Pest and Disease Outbreaks
RESEARCH EFFORT CATEGORIES
BASIC |
70% |
APPLIED |
20% |
DEVELOPMENTAL |
10% |
KEYWORDS: melanoma; macrophages; swine; tumors; molecular biology; genetics; gene mapping; lymphocytes; immune response; human diseases; biochemistry; human physiology; tumorigenesis; signals; animal models; gene analysis; cytokines; apoptosis; telomerase; blood cells; gene function
PROGRESS: Sep 11, 2001 TO Sep 10, 2006
The incidence of melanoma is rising rapidly, especially in the southwest. This research addresses the genetic parameters leading to the expression of melanoma and the physiological parameters producing spontaneous regression. The purpose of this research is to identify the genes responsible for the expression of melanoma, the specific role the genes play and the immunological mechanisms responsible for the spontaneous regression. With this information, final treatments will be developed. The overall objectives are to determine the cellular and molecular signals that initiate tumorigenesis and regression in a swine model of melanoma. The projects are: 1) to map and determine the genes responsible for expression of the tumor, 2) to determine the relative contribution of cytokines, macrophages and lymphocytes to regression, 3) to determine the roles of apoptosis and lack of telomerase to regression. A herd of Sinclair swine will be maintained from which tumor tissue and
white blood cells will be collected for use in various in vitro experiments. DNA will be used to map genes responsible for melanoma, once genes are identified and located, the role of that gene in causing the tumor will be investigated. As mechanisms are identified from the in vitro experiments, in vivo perturbations will be initiated to confirm and extend our observations. It is expected that the identification of a gene(s) involved in the expression and regression of these swine malignant melanomas will lead to the identification and control of proteins responsible. It is envisioned that as we identify the specific molecules responsible, it will provide unique avenues to accurately and succinctly design novel therapies for this devastating cancer. Use of the Sinclair swine/melanoma line has been successful and upon Dr. Amoss' retirement, the herd was transferred to the University of Nevada, Reno for continued research.
IMPACT: 2001-09-11 TO 2006-09-10
It is expected that the identification of a gene(s) involved in the experession and regression of these swine malignant melanomas will lead to the identification and control of the proteins responsible. It is envisioned that as we identify the specific molecules responsible, it will provide unique avenues to accurately and succinctly design novel therapies for this devastating cancer.
PUBLICATION INFORMATION: 2001-09-11 TO 2006-09-10
No publications reported this period
PROJECT CONTACT INFORMATION
NAME: |
Amoss, M. S. |
PHONE: |
979-845-5906 |
FAX: |
979-458-3361 |
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