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Hildebrand M, Kim S, Shi D, Scott. K and Subramaniam S (2009) 3D Imaging of Diatoms with Ion-abrasion Scanning Electron Microscopy .
J. Struct. Biol. (in press). |
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Heymann J, Shi D, Kim S, Bliss D, Milne JLS and Subramaniam S (2008)
3D Imaging of Mammalian Cells With Ion-Abrasion Scanning Electron Microscopy.
J. Struct. Biol. (in press). |
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Narasimha R, Ouyang H, Gray A, McLaughlin SW and Subramaniam S (2008)
Automatic joint classification and segmentation of whole cell 3D images.
Pattern Recognition (in press). |
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Khursigara C, Wu X, Zhang P, Lefman J and Subramaniam S (2008)
Role of HAMP domains in chemotaxis signaling by bacterial chemoreceptors.
Proc. Natl. Acad. Sci. USA (in press). |
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Khursigara CM, Wu X and
Subramaniam S (2008)
Chemoreceptors in Caulobacter crescentus: trimers of receptor dimers
in a partially ordered hexagonally packed array.
J. Bacteriol. 190(20): 6805-6810. |
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Liu J, Bartesaghi A, Borgnia,
MJ, Sapiro G and Subramaniam S
(2008)
Molecular architecture of native gp120 trimers.
Nature 455: 109-113. |
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Narasimha R, Aganj I, Borgnia
MJ, Bennett A, Zabransky D, Sapiro G, McLaughlin S, Milne
JLS and Subramaniam S (2008)
Evaluation of denoising algorithms for biological electron tomography.
J. Struct. Biol. 164(1): 7-17. |
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Borgnia MJ, Subramaniam
S and Milne JLS (2008)
Three-Dimensional Imaging of the Highly
Bent Architecture of Bdellovibrio bacteriovorus by Using Cryo-Electron
Tomography.
J. Bacteriol. 190(7): 2588-2596. |
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Bartesaghi A, Sprechman
P, Liu J, Randall G, Sapiro G and Subramaniam S (2008) Classification
and 3D averaging with missing wedge correction in biological electron
tomography.
J. Struct. Biol. 162(3): 436-450. |
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Nanavati, D,
Gucek M, Milne JLS, Subramaniam S and Markey S (2008)
Stoichiometry and absolute quantification of proteins with mass spectrometry
using fluorescent and isotope labeled concatenated peptide standards.
Mol. Cell. Proteomics 7: 442-447. |
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Lengyel JS, Milne JLS and
Subramaniam S. (2008)
Electron tomography in Nanoparticle Imaging
and Analysis.
Nanomedicine 3(1): 125-131. |
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Lengyel S, Stott KM,
Wu X, Brooks, BR, Balbo A, Schuck P, Perham RN, Subramaniam
S and Milne JLS (2008)
Extended polypeptide linkers establish
the spatial architecture of a pyruvate dehydrogenase.
Structure, 16(1): 93-103. |
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Subramaniam S, Bartesaghi
A, Liu J, Bennett AE and Sougrat R (2007)
Electron tomography of viruses.
Current Opinion in Structural Biology 17(5): 596-602. |
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Liu J, McBride MJ, Subramaniam
S (2007)
Cell-surface filaments of the gliding bacterium Flavobacterium
johnsoniae revealed by cryo-electron tomography.
J. Bacteriol. 189(20): 7503-7506. |
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Bennett AE, Liu J, Van
Ryk D, Bliss D, Arthos J, Henderson RM and Subramaniam S (2007)
Cryoelectron tomographic analysis of an HIV neutralizing protein and its complex
with native viral gp120.
J. Biol. Chem. 282(38): 27754-27759. |
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Sougrat R, Bartesaghi
A, Lifson JD, Benett AE, Zabransky D and
Subramaniam S (2007)
Electron tomography of the contact between
T-cells and SIV/HIV-1: Implications for viral entry.
PLoS Pathogens 3(5): e63. |
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Lefman J, Morrison R and
Subramaniam S (2006)
Automated 100-Position Specimen Loader and Image
Acquisition System for Transmission Electron Microscopy
J. Struct. Biol. 158(3): 318-326. |
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Zhang P, Khursigara
CM, Hartnell L and Subramaniam S (2007)
Direct visualization
of E.coli chemotaxis receptor arrays using cryo-electron microscopy.
Proc. Natl. Acad. Sci. USA 104(10): 3777-3781. |
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Zhang P, Weis RM, Peters PJ and Subramaniam S (2007)
Electron tomography
of bacterial chemotaxis receptor assemblies.
Meth. Cell Biol. 79:
373-384. |
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Subramaniam S (2006)
The
SIV surface spike imaged by electron tomography: One leg or three?
PLoS Pathog 2(8): e91. |
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Heymann J, Hayles
M, Gestmann I, Giannuzzi L, Lich L and Subramaniam S (2006)
Site-specific
3D Imaging of Cells and Tissues With a Dual Beam Microscope.
J. Struct. Biol. 155(1): 63-73. |
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Milne JLS, Wu X,
Borgnia MJB, Lengyel JS, Brooks BR, Shi D, Perham RN
and Subramaniam S (2006)
Molecular Structure Of A 9 Mda Icosahedral
Pyruvate Dehydrogenase Sub-Complex Containing The E2 And E3 Enzymes
Using Cryo-Electron Microscopy.
J. Biol. Chem. 281(7): 4364-4370. |
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Bartesaghi A, Sapiro
G and Subramaniam S (2005)
An energy-based 3D segmentation approach
for the quantitative interpretation of electron tomograms.
IEEE Trans. Image Proc. 14(9): 1314-1323. |
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Subramaniam S (2005)
Bridging
the imaging gap: Visualizing subcellular architecture with electron
tomography.
Curr. Opin.Microbiology
8(3): 316-322. |
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Zhang P, Land W,
Lee S, Juliani J, Lefman J, Smith S, Germain D, Kessel
M, Leapman R, Rouault TA and Subramaniam S (2005)
Electron
tomography of degenerating neurons in mice with abnormal regulation
of iron metabolism.
J. Struct. Biol. 150(2): 144-153. |
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Zhang P, Bos E, Heymann
J, Gnaegi H, Kessel M, Peters P and Subramaniam S (2004)
Direct
visualization of receptor arrays in frozen-hydrated sections and
plunge-frozen specimens of E.coli engineered to overproduce the chemotaxis
receptor.
Tsr J. Microsc. 216(1): 76-83. |
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Lefman J, Zhang P,
Hirai T, Weis RM, Juliani J, Bliss D, Kessel M, Bos E,
Peters PJ and Subramaniam S (2004)
3D electron microscopic
imaging of membrane invaginations in E.coli overproducing the chemotaxis
receptor Tsr.
J. Bacteriol 186(15): 5052-5061. |
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Shen W,
Zhang P, Germain D, Rouault T and Subramaniam S (2004)
A
template propagation method for segmentation of filamentous structures
in electron tomograms.
IEEE ISBI 2004 (Biomedical Imaging). |
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Bartesaaghi A, Sapiro
G, Lee S, Lefman J and Subramaniam S (2004)
A new approach
for 3D segmentation of cellular tomograms obtained using three-dimensional
electron microscopy.
IEEE ISBI 2004 (Biomedical Imaging). |
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Borgnia MJ, Shi D, Zhang P and Milne JLS (2004)
Visualization of
a-helical features in a density map constructed using 9 molecular images of
the 1.8 MDa icosahedral core of pyruvate dehydrogenase.
J. Struct. Biol 147(2): 136-145. |
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Subramaniam S and Milne
JLS (2004)
Three-dimensional electron microscopy at molecular
resolution.
Ann. Rev. Biophys. Biomol. Struct. 33: 141-155. |
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Wu X, Milne JLS, Borgnia M, Rostapshov A, Subramaniam S and Brooks BR (2003)
A core-weighted fitting method for docking atomic structures
into low resolution maps: Application to electron cryo-microscopy.
J Struct Biol. 141(1): 63-76. |
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Hirai T, Heymann J
and Subramaniam S (2003)
A structural model for 12-helix transporters
in the Major Facilitator Superfamily.
J Bacteriol. 185(5): 1712-1718. |
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Zhang P, Borgnia M,
Mooney P, Shi D, Pan M, O'Herron P, Mao A, Brogan D,
Milne JLS and Subramaniam S (2003)
Automated image acquisition
and processing using a new generation of 4K x 4K CCD cameras for
cryo electron microscopic studies of macromolecular assemblies.
J Struct Biol. 143(2): 135-144. |
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Weis RM, Hirai T,
Chalah A, Kessel M, Peters PJ and Subramaniam S (2003)
Electron
microscopic analysis of membrane assemblies formed by the bacterial
chemotaxis receptor Tsr.
J Bacteriol. 185(12): 3636-3643. |
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Subramaniam S, Zhang
P, Lefman J, Juliani J and Kessel M (2003)
Electron tomography:
A powerful tool for three-dimensional cellular microscopy.
ASM News 60: 240-245. |
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Hirai T and Subramaniam
S (2003)
Structural insights into the mechanism of proton pumping
by bacteriorhodopsin.
FEBS Lett. 545(1): 2-8. |
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Heymann J, Hirai T,
Shi D and Subramaniam S (2003)
Projection structure of the bacterial
oxalate transporter at 3.4 Å resolution.
J Struct Biol. 144(3): 320-326. |
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Subramaniam S, Hirai
T and Henderson R (2002)
From structure to mechanism: Electron
crystallographic analysis of bacteriorhodopsin.
Philos Transact Ser A Math Phys Eng Sci. 360(1794): 859-874. |
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Hirai T, Heymann J,
Shi D, Sarker R, Maloney PC and Subramaniam S (2002)
Three
dimensional structure of a bacterial oxalate transporter.
Nat Struct Biol. 9(8): 597-600. |
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Milne JLS, Shi D,
Rosenthal PB, Sunshine J, Domingo G, Wu X, Broooks BR, Perham RN, Henderson R and Subramaniam S (2002)
Molecular
architecture and functional mechanism of an icosahedral pyruvate
dehydrogenase multienzyme complex.
EMBO J. 21(21): 5587-5598. |
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Tittor J, Paula S,
Subramaniam S, Heberle J, Henderson R and Oesterhelt D (2002)
Proton
Translocation by Bacteriorhodopsin in the Absence of Substantial
Conformational Changes.
J Mol Biol. 319(2): 555-65. |
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Zhang P, Beatty A,
Milne JLS and Subramaniam S (2001)
Automated data collection with a Tecnai 12 electron microscope:
Applications for molecular imaging by cryo-microscopy.
J Struct Biol. 135(3): 251-261. |
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Heymann J, Sarker
R, Hirai T, Shi D, Milne JLS, Maloney PC and Subramaniam
S (2001)
Projection structure and molecular architecture of OxlT, a membrane
transporter.
EMBO J. 20(16): 4408-4413. |
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Subramaniam S and Henderson R (2000)
Crystallographic Analysis of Protein Conformational Changes in the
Bacteriorhodopsin Photocycle.
Biochim Biophys Acta 1460(1): 157-165. |
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Subramaniam S and Henderson
R (2000)
Molecular mechanism for vectorial proton translocation by bacteriorhodopsin.
Nature 406(6796): 653-657. |
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Heymann J and Subramaniam
S (2000)
Integration of deletion mutants of bovine rhodopsin into the membrane
of the endoplasmic reticulum.
Mol Membr Biol 17(3): 165-174. |
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Faruqi AR and Subramaniam
S (2000)
Applications of CCD detectors in high resolution electron microscopy.
Q Rev Biophys 33(1): 1-27. |
|
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Subramaniam S and Henderson
R (1999)
Electron Crystallography of Bacteriorhodopsin with Millisecond Time
Resolution
J Struct Biol 128(1): 19-25. |
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Subramaniam S (1999)
The Structure of Bacteriorhodopsin: An Emerging Consensus.
Curr Opin Struct Biol 9(4): 462-468. |
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Subramaniam S, Lindahl
M, Bullough P, Faruqi AR, Tittor J, Oesterhelt D, Lanyi
J and Henderson R (1999)
Protein Conformational Changes in the Bacteriorhodopsin Photocycle.
J Mol Biol 287(1): 145-61. |
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Faruqi AR, Henderson
R and Subramaniam S (1999)
Cooled CCD Detector with Tapered Fibre Optics for Recording Electron
Diffraction Patterns.
Ultramicroscopy 75(4): 235-250. |
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