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BLASTX+BEAUTY Search Results

WU-BLAST 2.0 search of the National Center for Biotechnology Information's NR Protein Database.

BEAUTY post-processing provided by the Human Genome Sequencing Center, Baylor College of Medicine.

BEAUTY Reference:
Worley KC, Culpepper P, Wiese BA, Smith RF. BEAUTY-X: enhanced BLAST searches for DNA queries. Bioinformatics 1998;14(10):890-1. Abstract

Worley KC, Wiese BA, Smith RF. BEAUTY: an enhanced BLAST-based search tool that integrates multiple biological information resources into sequence similarity search results. Genome Res 1995 Sep;5(2):173-84 Abstract



RepeatMasker repeats found in sequence:

No Repeats Found.

Reference:  Gish, Warren (1994-1997).  unpublished.
Gish, Warren and David J. States (1993).  Identification of protein coding
regions by database similarity search.  Nat. Genet. 3:266-72.

Notice: statistical significance is estimated under the assumption that the equivalent of one entire reading frame in the query sequence codes for protein and that significant alignments will involve only coding reading frames.

Query= SSH3A09.SEQ(1>281) (256 letters)

  Translating both strands of query sequence in all 6 reading frames

Database: nr 505,245 sequences; 158,518,215 total letters.



     Observed Numbers of Database Sequences Satisfying
    Various EXPECTation Thresholds (E parameter values)

        Histogram units:      = 7 Sequences     : less than 7 sequences

 EXPECTation Threshold
 (E parameter)
    |
    V   Observed Counts-->
  10000 1876 413 |===========================================================
   6310 1463 371 |=====================================================
   3980 1092 208 |=============================
   2510  884 293 |=========================================
   1580  591 139 |===================
   1000  452 174 |========================
    631  278 116 |================
    398  162  56 |========
    251  106  47 |======
    158   59  13 |=
    100   46  11 |=
   63.1   35  15 |==
   39.8   20   2 |:
   25.1   18   6 |:
   15.8   12   2 |:
 >>>>>>>>>>>>>>>>>>>>>  Expect = 10.0, Observed = 10  <<<<<<<<<<<<<<<<<
   10.0   10   2 |:
   6.31    8   0 |
   3.98    8   1 |:
   2.51    7   1 |:
   1.58    6   0 |
   1.00    6   0 |
   0.63    6   0 |
   0.40    6   0 |
   0.25    6   1 |:
   0.16    5   0 |
   0.10    5   0 |
  0.063    5   0 |
  0.040    5   0 |
  0.025    5   0 |
  0.016    5   0 |
  0.010    5   0 |
 0.0063    5   0 |
 0.0040    5   0 |
 0.0025    5   1 |:


                                                                     Smallest
                                                                       Sum
                                                     Reading  High  Probability
Sequences producing High-scoring Segment Pairs:        Frame Score  P(N)      N
gi|4850385|gb|AAD31055.1|AC007357_4(AC007357) EST gb|... -1   116  3.6e-05   1
gi|6739522|gb|AAF27287.1|AF136007_1(AF136007) eukaryo... -1   116  3.6e-05   1
gi|6739539|gb|AAF27294.1|(AF145232) eukaryotic initia... -1    96  0.00040   1
gi|6224611|gb|AAF05869.1|AF021805_1(AF021805) eIF4B [... -1   104  0.00067   1
gi|6739518|gb|AAF27285.1|(AF136005) eukaryotic initia... -1    96  0.0023    1
gi|6739520|gb|AAF27286.1|AF136006_1(AF136006) eukaryo... -1    71  0.19      1
gi|6739515|gb|AAF27284.1|(AF136004) eukaryotic initia... -1    71  0.86      1
gi|2127792|pir||B64432capsular polysaccharide biosynt... +3    68  0.97      1
gi|7450263|pir||H69530conserved hypothetical protein ... +3    56  0.9991    1
gi|1176643|sp|Q09202|YP23_CAEELHYPOTHETICAL 25.9 KD P... +1    61  0.9999    1



Locally-aligned regions (HSPs) with respect to query sequence:

Locus_ID                Frame 3 Hits
gi|2127792             |        _____________________________________     
gi|7450263             |            ___________________________           
                        __________________________________________________
Query sequence:        |           |          |           |          |    | 86
                       0          20         40          60         80


Locus_ID                Frame 1 Hits
gi|1176643             |                       ___________________        
                        __________________________________________________
Query sequence:        |           |          |           |          |    | 86
                       0          20         40          60         80


Locus_ID                Frame -1 Hits
gi|4850385             |                          ________________________
gi|6739522             |                          ________________________
gi|6739539             |                          _______________________ 
gi|6224611             |                          _______________________ 
gi|6739518             |                          _______________________ 
gi|6739520             |                          _______________________ 
gi|6739515             |                          _______________________ 
                        __________________________________________________
Query sequence:        |           |          |           |          |    | 86
                       0          20         40          60         80

Use the and icons to retrieve links to Entrez:

E = Retrieve Entrez links (e.g., Medline abstracts, FASTA-formatted sequence reports).
R = Retrieve links to Related sequences (neighbors).
Use the icon (if present) to retrieve links to the Sequence Retrieval System (SRS).
Use the icon (if present) to retrieve links to the Ligand Enzyme and Chemical Compound Database .
Use the icon (if present) to retrieve links to the Protein Data Bank database.


to_Entrezto_Relatedto_Related >gi|4850385|gb|AAD31055.1|AC007357_4  (AC007357) EST gb|T22808 comes from this
            gene. [Arabidopsis thaliana]
            Length = 549

Frame -1 hits (HSPs):                                                 ____
                        __________________________________________________
Database sequence:     |             |             |            |         | 549
                       0           150           300          450

  Minus Strand HSPs:

 Score = 116 (40.8 bits), Expect = 3.6e-05, P = 3.6e-05
 Identities = 24/39 (61%), Positives = 31/39 (79%), Frame = -1

Query:   256 TGDMWMRPSDDRRQFQGGSRERGWFSSGSRNRSTS-RERW 140
             TGD W RP DDRR FQG S+ERG+F++ + +RS+S RE W
Sbjct:   511 TGDNWPRPVDDRRNFQG-SKERGFFNNRNFDRSSSAREGW 549


to_Entrezto_Relatedto_Related >gi|6739522|gb|AAF27287.1|AF136007_1  (AF136007) eukaryotic initiation factor 4B
            [Arabidopsis thaliana]
            Length = 549

Frame -1 hits (HSPs):                                                 ____
                        __________________________________________________
Database sequence:     |             |             |            |         | 549
                       0           150           300          450

  Minus Strand HSPs:

 Score = 116 (40.8 bits), Expect = 3.6e-05, P = 3.6e-05
 Identities = 24/39 (61%), Positives = 31/39 (79%), Frame = -1

Query:   256 TGDMWMRPSDDRRQFQGGSRERGWFSSGSRNRSTS-RERW 140
             TGD W RP DDRR FQG S+ERG+F++ + +RS+S RE W
Sbjct:   511 TGDNWPRPVDDRRNFQG-SKERGFFNNRNFDRSSSAREGW 549


to_Entrezto_Relatedto_Related >gi|6739539|gb|AAF27294.1|  (AF145232) eukaryotic initiation factor 4B
            [Arabidopsis thaliana]
            Length = 93

Frame -1 hits (HSPs):                                   __________________
                        __________________________________________________
Database sequence:     |          |         |          |          |       | 93
                       0         20        40         60         80

  Minus Strand HSPs:

 Score = 96 (33.8 bits), Expect = 0.00040, P = 0.00040
 Identities = 21/37 (56%), Positives = 29/37 (78%), Frame = -1

Query:   250 DMWMRPSDD-RRQFQGGSRERGWFSSGSRNRSTSRERW 140
             D W+RP+++ RR FQG ++ERG+FS    NRS+SRE W
Sbjct:    61 DAWVRPANEQRRNFQG-TKERGFFS----NRSSSREGW 93


to_Entrezto_Relatedto_Related >gi|6224611|gb|AAF05869.1|AF021805_1  (AF021805) eIF4B [Arabidopsis thaliana]
            Length = 531

Frame -1 hits (HSPs):                                                 ____
                        __________________________________________________
Database sequence:     |              |             |             |       | 531
                       0            150           300           450

  Minus Strand HSPs:

 Score = 104 (36.6 bits), Expect = 0.00067, P = 0.00067
 Identities = 23/37 (62%), Positives = 29/37 (78%), Frame = -1

Query:   250 DMWMRPSDD-RRQFQGGSRERGWFSSGSRNRSTSRERW 140
             D W+RP+D+ RR FQG S+ERG+FS    NRS+SRE W
Sbjct:   499 DAWVRPADEQRRNFQG-SKERGFFS----NRSSSREGW 531


to_Entrezto_Relatedto_Related >gi|6739518|gb|AAF27285.1|  (AF136005) eukaryotic initiation factor 4B
            [Arabidopsis thaliana]
            Length = 325

Frame -1 hits (HSPs):                                               ______
                        __________________________________________________
Database sequence:     |       |       |      |       |       |       |   | 325
                       0      50     100    150     200     250     300

  Minus Strand HSPs:

 Score = 96 (33.8 bits), Expect = 0.0023, P = 0.0023
 Identities = 21/37 (56%), Positives = 29/37 (78%), Frame = -1

Query:   250 DMWMRPSDD-RRQFQGGSRERGWFSSGSRNRSTSRERW 140
             D W+RP+++ RR FQG ++ERG+FS    NRS+SRE W
Sbjct:   293 DAWVRPANEQRRNFQG-TKERGFFS----NRSSSREGW 325


to_Entrezto_Relatedto_Related >gi|6739520|gb|AAF27286.1|AF136006_1  (AF136006) eukaryotic initiation factor 4B
            [Triticum aestivum]
            Length = 135

Frame -1 hits (HSPs):                                       ______________
                        __________________________________________________
Database sequence:     |                  |                 |             | 135
                       0                 50               100

  Minus Strand HSPs:

 Score = 71 (25.0 bits), Expect = 0.20, P = 0.19
 Identities = 16/37 (43%), Positives = 25/37 (67%), Frame = -1

Query:   250 DMWMRPSDDRRQFQGGSRERGWFSSG-SRNRSTSRERW 140
             + + +P ++R  F G SRERG F  G S +RS++R+ W
Sbjct:    99 ETYPKPVEERWGFHG-SRERGSFGGGGSSDRSSTRQGW 135


to_Entrezto_Relatedto_Related >gi|6739515|gb|AAF27284.1|  (AF136004) eukaryotic initiation factor 4B [Triticum
            aestivum]
            Length = 462

Frame -1 hits (HSPs):                                                _____
                        __________________________________________________
Database sequence:     |                |               |               | | 462
                       0              150             300             450

  Minus Strand HSPs:

 Score = 71 (25.0 bits), Expect = 2.0, P = 0.86
 Identities = 16/37 (43%), Positives = 25/37 (67%), Frame = -1

Query:   250 DMWMRPSDDRRQFQGGSRERGWFSSG-SRNRSTSRERW 140
             + + +P ++R  F G SRERG F  G S +RS++R+ W
Sbjct:   426 ETYPKPVEERWGFHG-SRERGSFGGGGSSDRSSTRQGW 462


to_Entrezto_Relatedto_Related >gi|2127792|pir||B64432  capsular polysaccharide biosynthsis protein M homolog -
            Methanococcus jannaschii >gi|1591712|gb|AAB99063.1| (U67549)
            capsular polysaccharide biosynthsis protein M [Methanococcus
            jannaschii]
            Length = 406

Frame  3 hits (HSPs):                              ________               
                        __________________________________________________
Database sequence:     |                  |                 |             | 406
                       0                150               300

  Plus Strand HSPs:

 Score = 68 (23.9 bits), Expect = 3.5, P = 0.97
 Identities = 22/63 (34%), Positives = 37/63 (58%), Frame = +3

Query:    45 KRL-EKTSN-KIVVLGSGHQKLKITLNPHKTAVHHLSLDVDLFLLPEENHPLSLLPP*NC 218
             KR+ EK  N K+++LG G  K K+     +  ++ L+L  +++LL  + +P   L   NC
Sbjct:   225 KRVTEKYPNAKLIILGDGELKNKL-----QELINKLNLQNNVYLLGMQKNPFKFLKHSNC 279

Query:   219 LL-SSL 233
              + SSL
Sbjct:   280 FVFSSL 285


to_Entrezto_Relatedto_Related >gi|7450263|pir||H69530  conserved hypothetical protein AF2248 - Archaeoglobus
            fulgidus >gi|2648278|gb|AAB89009.1| (AE000949) conserved
            hypothetical protein [Archaeoglobus fulgidus]
            Length = 77

Frame  3 hits (HSPs):   _____________________________                     
                        __________________________________________________
Database sequence:     |            |            |            |           | 77
                       0           20           40           60

  Plus Strand HSPs:

 Score = 56 (19.7 bits), Expect = 7.0, P = 1.0
 Identities = 15/44 (34%), Positives = 22/44 (50%), Frame = +3

Query:    69 KIVVLGSGHQKLKITLNPHKTAVHHLSLDVDLFLLPEENHPLSL 200
             KI V+G G  + K T +  K  V    LDV+L  + + N  + L
Sbjct:     2 KIKVVGPGCARCKATFDVVKKVVEKEGLDVELEYVTDMNEAIEL 45


to_Entrezto_Relatedto_Related >gi|1176643|sp|Q09202|YP23_CAEEL  HYPOTHETICAL 25.9 KD PROTEIN AH6.3 IN
            CHROMOSOME II >gi|7494683|pir||T18613 hypothetical protein AH6.3 -
            Caenorhabditis elegans >gi|3873631|emb|CAA88077.1| (Z48009) AH6.3
            [Caenorhabditis elegans]
            Length = 230

Frame  1 hits (HSPs):   ________                                          
Annotated Domains:      ____________________                ______________
                        __________________________________________________
Database sequence:     |          |          |          |          |      | 230
                       0         50        100        150        200
__________________

Annotated Domains:
   Entrez               Transmembrane region: POTENTIAL.         7..23
   PRODOM               PD064152: YP23_CAEEL                     1..90
   PRODOM               PD053296: YP23_CAEEL                     169..229
__________________


  Plus Strand HSPs:

 Score = 61 (21.5 bits), Expect = 9.1, P = 1.0
 Identities = 15/35 (42%), Positives = 21/35 (60%), Frame = +1

Query:   121 HTKLLFTISLLTLTY--FCCQKKTIPFPCYHLETV 219
             + ++LFT S+L+L Y  F C KK  P P    E+V
Sbjct:     3 YIQMLFTASILSLGYLVFICGKKKKPKPTASTESV 37


Parameters:
  filter=none
  matrix=BLOSUM62
  V=50
  B=50
  E=10
  gi
  H=1
  sort_by_pvalue
  echofilter

  ctxfactor=5.94

  Query                        -----  As Used  -----    -----  Computed  ----
  Frame  MatID Matrix name     Lambda    K       H      Lambda    K       H
   Std.    0   BLOSUM62                                 0.318   0.135   0.401  
   +3      0   BLOSUM62        0.318   0.135   0.401    0.338   0.152   0.475  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +2      0   BLOSUM62        0.318   0.135   0.401    0.349   0.145   0.509  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   +1      0   BLOSUM62        0.318   0.135   0.401    0.378   0.172   0.685  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -1      0   BLOSUM62        0.318   0.135   0.401    0.347   0.151   0.570  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -2      0   BLOSUM62        0.318   0.135   0.401    0.335   0.151   0.442  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a
   -3      0   BLOSUM62        0.318   0.135   0.401    0.373   0.161   0.626  
               Q=9,R=2         0.244   0.0300  0.180     n/a     n/a     n/a

  Query
  Frame  MatID  Length  Eff.Length     E    S W   T  X   E2     S2
   +3      0       84        84       10.  61 3  12 22  0.12    31
                                                    28  0.096   33
   +2      0       85        84       10.  61 3  12 22  0.12    31
                                                    28  0.096   33
   +1      0       85        84       10.  61 3  12 22  0.12    31
                                                    28  0.096   33
   -1      0       85        85       10.  61 3  12 22  0.12    31
                                                    28  0.098   33
   -2      0       85        84       10.  61 3  12 22  0.12    31
                                                    28  0.096   33
   -3      0       84        84       10.  61 3  12 22  0.12    31
                                                    28  0.096   33


Statistics:

  Database:  /usr/local/dot5/sl_home/beauty/seqdb/blast/nr
    Title:  nr
    Release date:  unknown
    Posted date:  8:50 PM CDT May 27, 2000
    Format:  BLAST
  # of letters in database:  158,518,215
  # of sequences in database:  505,245
  # of database sequences satisfying E:  10
  No. of states in DFA:  583 (57 KB)
  Total size of DFA:  135 KB (192 KB)
  Time to generate neighborhood:  0.01u 0.00s 0.01t  Elapsed: 00:00:00
  No. of threads or processors used:  4
  Search cpu time:  116.47u 1.15s 117.62t  Elapsed: 00:00:45
  Total cpu time:  116.51u 1.17s 117.68t  Elapsed: 00:00:45
  Start:  Wed Feb 14 15:31:46 2001   End:  Wed Feb 14 15:32:31 2001

Annotated Domains Database: March 14, 2000
Release Date: March 14, 2000