Lee SG, Bae YS; American Society for Microbiology. General Meeting.
Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8; 97: 537 (abstract no. T7).
Department of Microbiology, Hannam University, Daejeon, Korea.
We have identified a novel Jurkat cell-specific cDNA clone, named JKL-1, which shows a clear leucine zipper domain. JKL-1 expression was dramatically downregulated by transfection of tat, a potent transactivator of HIV-1 LTR, suggesting that tat may also act as a negative regulation factor in eukaryotic expression. To identify the mechanism of tat effects on JKL-1 expression, p53 was cotransfected with LTR-CAT into tat-expressing cells. P53 transfection markedy reduced tat-mediated transactivation, but the JKL-1 expression was not induced by p53. This means that p53-mediated tat-suppression is unlikely to be associated with the amounts of tat-expression or dysfunction of tat. Whereas, transfection of p53 increase the expression of JKL-1 in Jurkat cells, and the effect was reduced by co-transfection of tat, suggesting that tat and p53 suppress each other for their biological functions which are reversed as compared in HIV-1 replication. Detail mechanisms are under investigation with anti-sense tat and JKL-1 promoter study.
Publication Types:
Keywords:
- Animals
- Cats
- Gene Products, tat
- Genes, p53
- Genes, tat
- HIV Long Terminal Repeat
- HIV-1
- Humans
- Jurkat Cells
- Promoter Regions (Genetics)
- Trans-Activation (Genetics)
- Trans-Activators
- Transfection
- Tumor Suppressor Protein p53
- genetics
Other ID:
UI: 102235411
From Meeting Abstracts