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Summary
The characteristics of an isolated in situ fish heart was investigated. A Pagothenia borchgrevinki fish was killed and its heart was exposed and isolated from its blood supply, but left in place within the fish carcass. Cannulae were inserted through an hepatic vein (input) and ventral aorta (output). These allowed monitoring of rate and pressure and allowed perfusion of ringer. Power and Starling curves were determined, plus the effects of the drugs acetylcholine and adrenaline. The innervation of the heart via the vagus nerve was also investigated. Blood and muscle tissue was collected for biochemical work. Levels of the antioxidants α-tocopherol and vitamin C were measured using HPLC methods and total lipids were extracted from both muscle and blood and analysed.

Geographic Coverage Spatial coordinates   

N: -77.85

S: -77.85

E: 166.75

W: 166.75

Temporal Coverage

Start Date: 1996-11-01 Stop Date: 1996-12-31 Start Date: 1998-11-01 Stop Date: 1998-12-31

Location Keywords

CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > MCMURDO SOUND

Science Keywords

BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH

ISO Topic Category

BIOTA

Platform

LABORATORY

Quality
The data is in the publications. Please contact the investigator for more information.

Ancillary Keywords

Pagothenia borchgrevinki heart rate blood chemistry

Data Set Progress

COMPLETE

Data Center

Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand Data Center URL: http://www.antarcticanz.govt.nz/ Data Center Personnel Name: SHULAMIT GORDON Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Personnel

BILL DAVISON Role: INVESTIGATOR Phone: +64 3 364 2029 Fax: +64 3 364 2590 Email: bill.davison at canterbury.ac.nz Contact Address: School of Biological Sciences University of Canterbury Private Bag 4800 City: Christchurch Country: New Zealand CEISHA POIROT Role: DIF AUTHOR Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: c.poirot at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand SHULAMIT GORDON Role: DIF AUTHOR Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Related URL

Link: VIEW RELATED INFORMATION Description: The investigators homepage

Publications/References
Sundin, L. Axelsson, M. Davison, W. Forster, M. 1999 Cardiovascular responses to adenosine in the Antarctic fish Pagothenia borchgrevinki. Journal of experimental biology 202: 2259-2267

Gieseg, S.P. Cuddihy, S. Hill, J.V. Davison, W. 2000 A comparison of plasma vitamin C and E levels in two Antarctic and two temperate water fish species. Comparative biochemistry and physiology B 125: 371-378

Creation and Review Dates

DIF Creation Date: 2009-03-31 Last DIF Revision Date: 2009-03-31

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