Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940816gel02
Unique Web Code: ysh06Srr
Tissue Aliquot Code: ocyuchry 002 #3 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559611.30.071516370.8267379.37.64.5596.97.30.0640369
25.219823.20.092185940.6584447.465.2197.54.10.0913131
35.8531236.60.12845441.0165111.49.35.85312.24.50.12843
46.5544952.60.080903281.0641711.99.86.55447.97.60.0789544
56.8548456.70.014189180.17423421.66.85485.17.60.0129221
67.3555164.60.047592760.496155.64.57.35550.960.0469483
78.1568480.20.059924990.9825841198.15685.19.40.0589645
88.4575388.30.14278361.4160615.9138.45752.25.70.140324
98.6579192.70.11969640.92776410.48.58.65791.24.50.117601
109.382696.80.15817851.3462915.112.39.3825.150.151598
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.203384.50.076220381.054594.43.54.20339.280.0743645
24.80413515.80.24696562.5044910.58.34.804135.75.90.241045
34.95916018.80.0979657111.941728.26.54.959156.511.30.0967501
45.34622426.30.018954260.2058510.90.75.346224.75.90.0195546
55.42223727.80.036759140.2570831.10.95.422236.84.10.035259
65.64327532.30.020049860.1959510.80.75.643274.45.70.0195069
75.78329935.10.014329730.08335130.40.35.783298.63.20.014608
85.85831236.60.042493550.3444331.41.15.8583124.60.0419201
95.97333539.30.48758143.1375113.210.45.973335.33.70.483567
106.06435341.40.27278341.981728.36.66.064352.64.20.264722
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.14536943.30.12098951.036054.43.46.145369.35.10.11495
126.19537944.50.11300230.6598462.82.26.1953793.40.108755
136.2539045.80.011371420.03043510.10.16.253901.60.010726
146.32140447.40.16480941.103844.63.76.321404.53.90.160268
156.48743751.30.10265881.454996.14.86.487437.38.40.0976234
166.517443520.10259920.1791180.80.66.517443.81.90.0527929
176.65546154.10.40539532.3876107.96.6554613.40.399123
186.79147655.90.024306320.09371290.40.36.791475.62.10.025624
196.85448356.70.073962830.3625761.51.26.854483.42.70.0759941
206.92149257.70.022882530.1610370.70.56.921490.530.0301675
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.99750258.90.034999630.2039850.90.76.997502.43.20.0361705
227.08751460.30.082344730.4990552.11.77.087514.13.40.0827653
237.16252461.50.54765243.79315.912.67.162523.63.90.553836
247.25253662.90.032906230.1380670.60.57.252537.12.60.0302818
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559310.90.05636380.7116589.17.24.5594.18.10.0495895
25.220123.60.068750150.5400426.95.55.2199.94.70.065411
35.8531036.40.11977150.97040112.49.95.85309.84.60.118628
46.55451530.084568670.9699712.49.96.55449.26.90.0794075
56.8548256.60.01203100.1675472.11.76.85483.710.20.0092275
67.3554864.40.035049260.3533834.53.67.35547.95.80.0343869
78.1568380.30.07358880.9870812.6108.156837.70.0724923
88.4575488.60.11921261.1558914.811.88.45753.55.60.11713
98.6579192.90.095585350.79015610.188.657904.70.0942461
109.382396.70.1381851.1643314.911.89.3821.94.90.133697
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.234404.70.031992170.3954122.11.94.23441.67.20.0311858
24.559110.70.039524860.37718121.84.5591.55.60.0377231
34.78512915.20.15188461.563768.17.54.785129.560.147964
44.92815217.90.0740649111.366057.16.54.928148.310.60.0726202
55.219623.10.030115150.2847111.51.45.2195.15.40.0298069
65.343221260.008920480.1110120.60.55.3432207.60.0082838
75.42823627.80.016714450.1415960.70.75.428238.15.30.0150879
85.6527532.40.00975360.08218550.40.45.65275.25.50.0083554
95.8531036.50.071614750.5998683.12.95.85309.84.90.0696077
105.98233639.50.33211842.2885411.910.95.982336.43.90.333401
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.08335641.90.17320841.282686.76.16.083356.34.20.17089
126.174374440.078013581.010695.24.86.174375.67.70.0736191
136.21438244.90.0769640.2267321.21.16.214382.83.50.0364118
146.32640447.50.079700740.5086692.62.46.3264043.70.0780651
156.48343551.20.07453270.77654143.76.483434.16.70.0653593
166.518442520.083332940.4263922.226.518441.73.80.0627106
176.57445153.10.072343630.4200132.226.574450.73.10.0756748
186.63945853.90.31341231.68828.88.16.639458.530.322032
196.75847155.40.007765310.01024510.106.758471.21.40.0042678
206.84148056.50.049971740.3345451.71.66.841480.24.30.0440692
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.98249958.70.021918130.1210820.60.66.982499.13.20.0212518
227.08551360.40.060626940.39026921.97.085511.83.50.0628482
237.14452161.30.48092443.1192116.214.97.144520.93.60.483321
247.2453462.80.019087720.06758270.40.37.24534.120.0186828
257.3554964.60.010207250.1241120.60.67.35547.95.30.0132082
268.1568680.70.026488790.39124221.98.15685.88.80.0251235
278.4575388.60.043644660.4069332.11.98.45752.45.50.0417341
288.6578792.60.037249340.2681851.41.38.65787.24.10.0372581
299.382096.50.057046950.490742.52.39.3818.85.50.0507251
PeakCounter: 29
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.265424.90.052347770.6864232.92.34.26541.27.30.0529698
24.81613115.40.2518762.58752118.84.816131.35.90.248539
34.97715718.50.0987605111.847397.96.34.977154.211.20.093428
45.37722426.40.024412460.2733371.20.95.377223.26.50.023756
55.43923527.70.042743340.3051161.315.439235.34.40.0394017
65.64527131.90.020057540.1519260.60.55.645271.44.50.0190742
75.77629434.60.069278370.7218963.12.45.776293.36.50.0626551
85.85530836.30.042438650.3781021.61.35.8553095.30.0404047
95.9933539.50.45619243.0998713.210.55.99335.43.90.45036
106.09135541.80.23978541.789687.66.16.091355.14.30.232896
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.18137343.90.10023840.6568942.82.26.181372.93.80.0969431
126.226382450.11725950.93640343.26.226381.94.80.109758
136.34640647.80.1381430.8447313.62.96.346405.73.50.137304
146.49743651.40.099980560.8933313.836.497434.95.70.0885332
156.53244352.20.10483340.6036832.626.532442.33.80.0886114
166.59745253.20.11125530.5709052.41.96.597451.62.80.115764
176.65845954.10.40748132.250329.67.66.658459.330.417301
186.77147255.60.017197720.06494330.30.26.7714722.10.0175223
196.8548156.70.060085740.3553391.51.26.85480.93.50.0571834
206.98249958.80.036059230.1951590.80.76.982499.630.0365985
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.09351460.50.073753640.5163852.21.87.093512.13.60.0800422
227.14452161.40.55163743.6439915.512.47.144521.33.70.557809
237.2453462.90.031260420.1238390.50.47.24534.42.30.0304554
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55849.90.050776370.5735687.86.34.5584.37.20.0450645
25.218922.30.067570650.5419237.365.2188.64.60.0662087
35.8530335.80.12271550.98282813.310.95.85302.84.50.122076
46.5544452.40.081694371.0002213.5116.55443.47.10.0795481
56.8548056.70.010530280.1323061.81.56.85479.27.50.0099191
67.3554864.70.034988660.3501664.73.97.355485.90.0335975
78.1567980.20.055463680.77741310.58.68.156797.80.0561978
88.45745880.11624161.167115.812.98.45744.25.70.115462
98.6578392.40.095728950.75890810.38.48.65782.24.60.0933437
109.381696.30.13152951.1010214.912.29.3815.94.90.127538
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic

 
Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 106
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 29 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse   |   RFE Tutorial   |   Seafoods   |   CFSAN


Last updated on 2003-FEB-27 by bjt/frf/nln