RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Theragra chalcogramma
FDA Seafood List Common Name: Walleye Pollock
FDA Seafood List Market Name: Pollock (Alaska Pollock)
Unique IEF Gel Code: 19970430gel03
Unique Web Code: wyg04Srr
Tissue Aliquot Code: therchal F96-005 #07m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	1.7	0.113982	15	2.8744	10.4	7.1	3.5	39.4	15	0.108088
2	4.55	380	17.1	0.19886	8	2.4716	9	6.1	4.55	380.8	7.9	0.176681
3	5.2	568	25.5	0.259682	7	2.92254	10.6	7.2	5.2	567.2	6.6	0.249596
4	5.85	862	38.7	0.307459	7	3.99613	14.5	9.9	5.85	862.1	7.4	0.305709
5	6.55	1246	56	0.17394	10	2.78471	10.1	6.9	6.55	1245.8	9.7	0.161289
6	6.85	1320	59.3	0.136533	8	1.86026	6.7	4.6	6.85	1320.5	7.9	0.132501
7	7.35	1508	67.7	0.179803	10	3.12875	11.3	7.7	7.35	1506.7	10.1	0.175384
8	8.15	1799	80.8	0.0560699	16	1.61111	5.8	4	8.15	1801.5	16.4	0.0553348
9	8.45	1927	86.6	0.0867752	10	1.55262	5.6	3.8	8.45	1927.7	10.3	0.0851215
10	8.65	1999	89.8	0.089727	9	1.43134	5.2	3.5	8.65	1997.8	9.3	0.0870927
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2214	99.5	0.225067	7	2.95704	10.7	7.3	9.3	2213.7	7.3	0.227034

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.029	212	9.5	0.166497	13	3.60228	10.6	6.3	4.029	212.2	12.9	0.157077
2	4.235	278	12.5	0.237704	18	7.84555	23	13.8	4.235	282.4	18.1	0.245057
3	4.366	320	14.4	0.181296	13	3.78058	11.1	6.6	4.366	317.9	13.4	0.158649
4	4.973	502	22.6	0.0279441	7	0.408339	1.2	0.7	4.973	504.9	7.1	0.0323816
5	5.1	539	24.2	0.0646752	12	1.20633	3.5	2.1	5.1	539.8	12.2	0.0558974
6	5.245	589	26.5	0.0553157	13	1.26427	3.7	2.2	5.245	586.9	13.5	0.0530009
7	5.347	636	28.6	0.360247	8	4.51493	13.2	7.9	5.347	638.3	7.6	0.333358
8	5.486	700	31.5	0.0914394	11	1.51133	4.4	2.7	5.486	697.9	10.6	0.0804913
9	5.865	876	39.4	0.244696	11	4.53715	13.3	8	5.865	875	11.1	0.231243
10	5.946	920	41.3	0.152524	11	2.58894	7.6	4.6	5.946	920.4	10.6	0.138418
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.076	990	44.5	0.167019	5	1.34938	4	2.4	6.076	990.5	4.6	0.165436
12	6.172	1042	46.8	0.128766	4	0.935102	2.7	1.6	6.172	1042.1	4.3	0.123531
13	6.298	1110	49.9	0.0691587	5	0.598217	1.8	1.1	6.298	1110.2	4.9	0.0685646

PeakCounter: 13

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	2.1	0.103786	13	2.51183	8.4	5.8	3.5	51.8	13.3	0.106389
2	4.55	378	17	0.188235	9	2.70014	9	6.3	4.55	379.2	8.7	0.174478
3	5.2	568	25.5	0.2239	8	3.14459	10.5	7.3	5.2	568.8	8.2	0.215751
4	5.85	874	39.3	0.267546	9	4.35923	14.5	10.1	5.85	873.5	9.2	0.267296
5	6.55	1246	56	0.179658	8	2.45899	8.2	5.7	6.55	1245.5	8.3	0.167488
6	6.85	1326	59.6	0.148729	8	1.97874	6.6	4.6	6.85	1325.4	7.9	0.140734
7	7.35	1509	67.9	0.205966	9	3.2719	10.9	7.6	7.35	1507.3	9.1	0.202639
8	8.15	1796	80.8	0.0577119	16	1.53518	5.1	3.6	8.15	1797	15.9	0.0544951
9	8.45	1920	86.3	0.103705	11	1.93233	6.4	4.5	8.45	1920.6	10.8	0.101199
10	8.65	1990	89.5	0.108606	9	1.76937	5.9	4.1	8.65	1989.7	9.5	0.105622
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2202	99	0.288164	9	4.41036	14.7	10.2	9.3	2201.7	9.1	0.273735

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	53	2.4	0.102005	15	2.66981	5.1	3.5	3.5	58.1	15.4	0.0977286
2	3.995	218	9.8	0.160836	12	3.35701	6.4	4.4	3.995	218.6	12.3	0.153395
3	4.175	278	12.5	0.227432	22	8.82032	16.9	11.7	4.175	287	22.2	0.224472
4	4.325	328	14.8	0.181783	11	2.90335	5.6	3.8	4.325	327.5	11	0.149552
5	4.55	403	18.1	0.0798396	9	1.13713	2.2	1.5	4.55	404.8	8.9	0.0718702
6	4.947	516	23.2	0.0554077	8	0.703854	1.3	0.9	4.947	515	7.6	0.0520635
7	5.059	548	24.7	0.0555988	11	1.00132	1.9	1.3	5.059	548.8	10.6	0.0531648
8	5.2	588	26.5	0.134445	10	2.1671	4.2	2.9	5.2	588.2	9.9	0.123287
9	5.236	605	27.2	0.0502443	4	0.39624	0.8	0.5	5.236	604.1	4.4	0.0504192
10	5.297	634	28.5	0.420989	4	2.73413	5.2	3.6	5.297	633.9	3.9	0.394564
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.329	649	29.2	0.188349	11	2.79067	5.3	3.7	5.329	649.3	10.7	0.146837
12	5.424	694	31.2	0.108538	8	1.42702	2.7	1.9	5.424	694.6	8	0.100395
13	5.778	862	38.8	0.321297	8	4.37987	8.4	5.8	5.778	863.7	7.8	0.316794
14	5.85	896	40.3	0.114927	13	2.38654	4.6	3.2	5.85	895	13.5	0.0999902
15	5.882	912	41	0.185009	7	2.05599	3.9	2.7	5.882	913.8	7	0.165392
16	6.066	1003	45.1	0.173008	6	1.82812	3.5	2.4	6.066	1002.2	5.9	0.174183
17	6.166	1052	47.3	0.149703	4	1.08494	2.1	1.4	6.166	1051.1	4	0.152537
18	6.307	1122	50.5	0.119416	4	0.827366	1.6	1.1	6.307	1122.3	4.2	0.110837
19	6.85	1328	59.7	0.104192	8	1.3094	2.5	1.7	6.85	1327.3	7.7	0.0964034
20	7.35	1508	67.8	0.180995	8	2.56313	4.9	3.4	7.35	1507.6	8.1	0.178669
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.15	1790	80.5	0.0144807	15	0.357907	0.7	0.5	8.15	1784.8	14.7	0.0137034
22	8.45	1917	86.2	0.0616701	13	1.32386	2.5	1.8	8.45	1917.3	12.5	0.0595257
23	8.65	1988	89.4	0.0682725	9	1.08678	2.1	1.4	8.65	1986.9	9.3	0.0662315
24	9.3	2198	98.9	0.209195	8	2.87674	5.5	3.8	9.3	2197.8	8.1	0.200686

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.969	210	9.5	0.197597	12	4.00756	10.3	6.3	3.969	211.4	11.8	0.191125
2	4.148	268	12.1	0.203996	25	8.87219	22.9	14	4.148	276.9	24.9	0.200894
3	4.314	322	14.5	0.191885	8	2.55856	6.6	4	4.314	321	8.2	0.176189
4	4.926	504	22.7	0.0536993	6	0.52173	1.3	0.8	4.926	503.9	5.8	0.0506779
5	5.015	529	23.8	0.0595158	30	2.57205	6.6	4.1	5.015	549.6	30	0.0483717
6	5.219	590	26.6	0.119289	7	1.12879	2.9	1.8	5.219	588.2	7.4	0.0866071
7	5.276	618	27.8	0.509241	4	3.56589	9.2	5.6	5.276	618.8	4.1	0.488793
8	5.313	636	28.6	0.213344	7	2.46205	6.3	3.9	5.313	638.2	7.2	0.19378
9	5.4	678	30.5	0.0707035	10	1.15605	3	1.8	5.4	680.3	9.7	0.0672254
10	5.747	846	38.1	0.425605	6	4.56878	11.8	7.2	5.747	846.2	6.3	0.409277
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.854	898	40.4	0.250563	8	3.22934	8.3	5.1	5.854	897.8	7.8	0.232869
12	6.046	992	44.6	0.224369	5	1.89298	4.9	3	6.046	992.3	4.7	0.22561
13	6.145	1040	46.8	0.217042	4	1.28953	3.3	2	6.145	1040.2	3.5	0.207748
14	6.292	1112	50	0.155431	4	0.984716	2.5	1.6	6.292	1111.4	3.7	0.148298

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	63	2.8	0.163501	15	4.282	13.9	10.6	3.5	68.1	14.7	0.164571
2	4.55	394	17.7	0.17891	9	2.7054	8.8	6.7	4.55	393.8	9.1	0.166857
3	5.2	574	25.8	0.219604	9	3.43938	11.2	8.5	5.2	573.9	8.9	0.217125
4	5.85	896	40.3	0.351393	7	4.21805	13.7	10.4	5.85	896.4	6.8	0.349259
5	6.55	1234	55.6	0.16454	9	2.51821	8.2	6.2	6.55	1232.8	9.4	0.150538
6	6.85	1324	59.6	0.145912	10	2.34336	7.6	5.8	6.85	1323.7	9.6	0.138011
7	7.35	1506	67.8	0.184149	10	3.06161	9.9	7.6	7.35	1503.6	9.7	0.1783
8	8.15	1802	81.1	0.0546702	18	1.71874	5.6	4.2	8.15	1802.4	17.7	0.0547836
9	8.45	1917	86.3	0.103267	12	2.121	6.9	5.2	8.45	1917.3	11.9	0.100237
10	8.65	1986	89.4	0.101841	9	1.60705	5.2	4	8.65	1985.3	9.5	0.0956977
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2204	99.2	0.178268	9	2.76394	9	6.8	9.3	2203.3	8.6	0.180961

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 84

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 10.400 4.550 9.000 5.200 10.600 5.850 14.500 6.550 10.100 6.850 6.700 7.350 11.300 8.150 5.800 8.450 5.600 8.650 5.200 9.300 10.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator
4.029 10.600 4.235 23.000 4.366 11.100 4.973 1.200 5.100 3.500 5.245 3.700 5.347 13.200 5.486 4.400 5.865 13.300 5.946 7.600 6.076 4.000 6.172 2.700 6.298 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 9 PeakCount: 13 Return to Lane Navigator
3.500 8.400 4.550 9.000 5.200 10.500 5.850 14.500 6.550 8.200 6.850 6.600 7.350 10.900 8.150 5.100 8.450 6.400 8.650 5.900 9.300 14.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 5.100 3.995 6.400 4.175 16.900 4.325 5.600 4.550 2.200 4.947 1.300 5.059 1.900 5.200 4.200 5.236 0.800 5.297 5.200 5.329 5.300 5.424 2.700 5.778 8.400 5.850 4.600 5.882 3.900 6.066 3.500 6.166 2.100 6.307 1.600 6.850 2.500 7.350 4.900 8.150 0.700 8.450 2.500 8.650 2.100 9.300 5.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 24 Return to Lane Navigator
3.969 10.300 4.148 22.900 4.314 6.600 4.926 1.300 5.015 6.600 5.219 2.900 5.276 9.200 5.313 6.300 5.400 3.000 5.747 11.800 5.854 8.300 6.046 4.900 6.145 3.300 6.292 2.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 14 Return to Lane Navigator
3.500 13.900 4.550 8.800 5.200 11.200 5.850 13.700 6.550 8.200 6.850 7.600 7.350 9.900 8.150 5.600 8.450 6.900 8.650 5.200 9.300 9.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Walleye Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.