Wang JJ, Chiou CT, Hu HM, Chuang CY, Chen CF, Gomez VF, Chang LR, Wang TM, Lee HK; International Conference on AIDS.

Int Conf AIDS. 1996 Jul 7-12; 11: 143 (abstract no. Mo.C.1507).

Inst. Biol & Anat, Inst. Preventive Medicine, National Defense Medical Center, Taipei, Taiwan. Fax: 2-3657481. E-mail: jjw@ndmcl. ndmctsgh. edu. tw.

Objective: To screen and identify HIV types of seropositive patients in Canchungo, Guine Bissau and Taiwan by ELISA, Serodia, Western blots and immunocytochemical assays. Methods: The serum samples included 24 HIV seropositives from Canchungo Hospital in 1994, 77 HIV seropositives among the community members in Canchungo area in 1995, and 60 AIDS patients from Taiwan in 1994-1995. Haemophiliac AIDS patients were also included as a high risk group in Taiwan. Combined HIV-1/-2 Biotest ELISA, Serodia HIV-1/-2 agglutination tests, various Western blot assays and confirmatory immunocytochemistry were applied. Results: According to the interpretative criteria of the American Red Cross, seroprevalence rates of HIV-1, HIV-1/-2 co-infection, HIV-2 and indeterminates among AIDS patient in Canchungo were identified to be: 8.3% (2/24), 16.6% (4/24), 54.2% (13/24), and 20.8% (5/24) respectively in 1994; and 7.7% (6/77), 33.8% (26/77), 49.3% (38/77) and 9.1% (7/77) respectively in 1995. The prevalence rate for HIV-1 was stable when compared to previous data obtained in 1987-1994. However, HIV-1/-2 co-infections were found with a 2-3.7 fold increase. In Taiwan, the seroprevalence rates of HIV types among AIDS patients were: 91.7% (55/60), 3.3% (2/60), 0 and 5% (3/60) respectively in 1994-1995. Supporting data were obtained from Vpr (HIV-1 specific) and Vpx (HIV-2 specific) immunocytochemical examinations on lymph nodes with light and electron microscopy. Conclusions: Increase of HIV-1/-2 co-infection cases in Guine Bissau have been identified. A reliable and economic method of HIV serodiagnosis was evaluated by using combined ELISA/Serodia assays in Western Africa. Furthermore, confirmatory Western blot assays have defined two cases of co-infections (i.e., HIV-2 patterns among HIV-1 AIDS patients) in Taiwan.

Publication Types:

• Meeting Abstracts

Keywords:

• AIDS Serodiagnosis
• Acquired Immunodeficiency Syndrome
• Africa, Western
• Agglutination Tests
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• HIV Infections
• HIV Seropositivity
• HIV Seroprevalence
• HIV-1
• HIV-2
• Humans
• Prevalence
• Seroepidemiologic Studies
• Taiwan

Other ID:

• 96921564

UI: 102217463

From Meeting Abstracts