Espinel-Ingroff A, Norris HA, Ghannoum MA, Killian S, Knapp C, Nugent E; American Society for Microbiology. General Meeting.

Abstr Gen Meet Am Soc Microbiol. 1998 May 17-21; 98: 178-9 (abstract no. C-285).

Med. Coll. of Virginia of Virginia Commonwealth Univ., Richmond, USA.

The availability of NCCLS standard guidelines for antifungal susceptibility testing of yeasts has improved interlaboratory agreement for MICs and allowed the establishment of breakpoints. However, NCCLS methods may not be the most efficient and convenient procedures for use in the clinical laboratory. We compared colorimetric MICS determined by the AccuMed Sensititre YeastOne Colorimetric Antifungal Panel with reference MICs by the NCCLS M27-A broth microdilution method. MICs of amphotericin B (A), fluconazole (FL), flucytosine (FC), itraconazole (1), and ketoconazole (K) were determined by both methods after 24 and 48 h of incubation for 582 Candida spp. clinical isolates. Colorimetric MICs of A corresponded to the first blue well (no growth) and MICs for the other agents to the first purple or blue well. Three sets of MIC pairs of each drug were compared (+/- 1 dil.), colorimetric 24 h MICs against 24 and 48 h reference MICs and the 48 h results by both methods. For A, the best agreement (88 to 100%) was seen after 48 h of incubation for the 6 species tested: Candida albicans (Ca), C. glabrata (Cgl), C. krusei (Ck) C. lusitaniae (C1), C. parapsilosis (Cp), and C. tropicalis (Ct.) When testing Ca, the agreement was higher for the 24 h (90 to 93%) MIC pairs than for the other pairs (56 to 71%) with FL, I, and K. Similar results were obtained with Fe, I, and K for the other species tested, but the differences were lower: 92 to 100% for 24 h MIC pairs and 73 to 100% for the other pairs. For FC, the agreement was more species and incubation time dependent: 98% between 24 h MICs pairs for Cgl, Clu, and Cp; 94 to 98% between 48 h MICs for Ca and Ct; and 97% between 24 h colorimetic and 48 h reference MICs for Ck. These data suggest the potential of the Sensititre Panel for use in the clinical laboratory for antifungal susceptibility testing of the Candida spp. evaluated.

Publication Types:

• Meeting Abstracts

Keywords:

• Amphotericin B
• Antifungal Agents
• Candida
• Candida albicans
• Candidiasis
• Evaluation Studies
• Fluconazole
• Flucytosine
• Itraconazole
• Ketoconazole
• Laboratories
• Miconazole
• Microbial Sensitivity Tests
• Research Design
• Yeasts
• methods

Other ID:

• 98349260

UI: 102226605

From Meeting Abstracts