Gene ID:HHVTW030
DNA Molecule Name:
1
GenBank ID:
2815498
Gene Name:
UL27
Definition:
virion glycoprotein B, gB, VP7 (VGLB_HSV2H)
Gene Start:
56117
Gene Stop:
53403
Gene Length:
2715
Molecular Weight*:
100214
pI*:
8.40
Net Charge*:
11.34
EC:
Functional Class:
virion entry and egress; cell membrane penetration
Gene Ontology:
Pathway: pathway table
Primary Evidence:
Norais,N., Tang,D., Kaur,S., Chamberlain,S.H., Masiarz,F.R.,Burke,R.L. and Marcus,F, Disulfide bonds of herpes simplex virus type 2 glycoprotein gB, J. Virol. 70 (11), 7379-7387 (1996), Medline: 97048015.
Becker Y, Computer prediction of antigenic and topogenic domains in HSV-1 and HSV-2 glycoprotein B (gB), Virus Genes 1992 Apr;6(2):131-41, Medline: 92271458.
Stuve,L.L., Brown-Shimer,S., Pachl,C., Najarian,R., Dina,D., Burke,R.L, Structure and expression of the herpes simplex virus type 2 glycoprotein gB gene, J. Virol. 61 (2), 326-335 (1987), Medline: 87112925.
Bzik,D.J., Debroy,C., Fox,B.A., Pederson,N.E. and Person,S., The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1, Virology 155 (2), 322-333 (1986), Medline: 87071654.
Secondary Evidence:
Rajcani J, Vojvodova A, The role of herpes simplex virus glycoproteins in the virus replication cycle, Acta Virol 1998 Apr;42(2):103-18, Medline: 98442215.
Chang YE, Menotti L, Filatov F, Campadelli-Fiume G, Roizman B, UL27.5 is a novel gamma2 gene antisense to the herpes simplex virus 1 gene encoding glycoprotein B, J Virol 1998 Jul;72(7):6056-64, Medline: 98285767.
McGeoch DJ, Cook S, Dolan A, Jamieson FE, Telford EA, Molecular phylogeny and evolutionary timescale for the family of mammalian herpesviruses, J Mol Biol 1995 Mar 31;247(3):443-58, Medline: 95230685.
Hutchinson L, Graham FL, Cai W, Debroy C, Person S, Johnson DC, Herpes simplex virus (HSV) glycoproteins B and K inhibit cell fusion induced by HSV syncytial mutants, Virology 1993 Oct;196(2):514-31, Medline: 93383376.
Yuhasz SA, Stevens JG, Glycoprotein B is a specific determinant of herpes simplex virus type 1 neuroinvasiveness, J Virol 1993 Oct;67(10):5948-54, Medline: 93381796.
McGeoch,D.J., Dalrymple,M.A., Davison,A.J., Dolan,A., Frame,M.C., McNab,D., Perry,L.J., Scott,J.E. and Taylor,P.,
The complete DNA sequence of the long unique region in the genome
of herpes simplex virus type 1, J. Gen. Virol. 69 (Pt 7), 1531-1574 (1988), Medline: 88274327.
Hammerschmidt,W., Conraths,F., Mankertz,J., Pauli,G., Ludwig,H. and Buhk,H.J, Conservation of a gene cluster including glycoprotein B in bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1), Virology 165 (2), 388-405 (1988), Medline: 88306231.
Pellett,P.E., Kousoulas,K.G., Pereira,L. and Roizman,B.,
Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants, J. Virol. 53 (1), 243-253 (1985), Medline: 85083254.
Comment:
gB is required for viral growth, virus infectivity and cell penetration. It forms a dimer and Roizman reports gamma expression regulation in HSV-1 (Roizman PNAS 93:11307,1996, Medline: 97030190).
In HSV-2 UL27 contains a tandem nucleotide repeat, given in genome cordinates at 56029 - 56056, with period/unit size 9, copy # 3.1, and 100% matches with no insert/deletes.
A second ORF in both HSV-1 and HSV-2 was identified by Chang et al.(1998) coding antisense to UL27 and designated UL27.5. The 10 cysteines in gB are linked by disulfide bonds, see Norais et al.(1996). A syn(syncytium) minus loci maps to the carboxyl terminus of UL27, see Hutchinson et al.(1993). Kosovsky et al. (1999) find gB to be essential for fusion between virion envelope and cell membrane in HSV-1.
Blast Summary: PSI-Blast Search
Residues 6-904 of UL27 are 86% similar using gapped BLAST to residues 14-904 of UL27 in Human herpesvirus 1 (strain 17) and 77% similar over 819/823 aa to Cercopithecine herpesvirus 1 and 2, 77% similar over 823 aa to Baboon herpesvirus 2 (AAA85648), 65% similar over 821/825 aa to Macropodid herpesvirus 1 and 2, with strong similarity to many other Alphaherpesvirinae and weaker similarity to Beta and Gamma herpesvirus.
Variants include AAB60546, AAB72100, P06763, AAB60547, AAB60545, AAA60540, VGBEK2.
Top Blast Hits: Updated monthly
Click here to view the entire PsiBlast results.
COGS Summary: COGS Search
None.
Blocks Summary: Blocks Search
Residues 139-161, 258-296, 359-390, 500-540, 541-573, 593-603, 605-647, 664-707 and 731-785 are members of blocks PF00606A-I,
Herpesvirus glycoprotein B.
ProDom Summary: Protein Domain Search
Residues 108-745 constitute a glycoprotein B domain as seen in VGLB_HSV2H and are 99% similar to domains seen in Q89920_VVVVV, O37453_VVVVV, VGLB_HSV2, Q69465_VVVVV, Q69464_VVVVV, and Q69095_VVVV. Residues 108-815 are 94% similar to a glycoprotein B domain as seen in VGLB_HSV11, VGLB_HSV1F, Q69076_VVVVV, Q69526_VVVVV, VGLB_HSV1P and VGLB_HSV1K.
Paralogs: Local Blast Search
None.
Pfam Summary: Pfam Search
Residues 105-818 make up the model Glycoprotein_B(PF00606), described as Herpesvirus Glycoprotein B.
Structural Feature(s):
| Feature Type | Start | Stop |
| eukaryotic signal | ||
| non-globular | ||
| non-globular | ||
| transmembrane | ||
| non-globular |
Top PDB Hits:
None.
Gene Protein Sequence:
MRGGGLICALVVGALVAAVASAAPAAPAAPRASGGVAATVAANGGPASRP
PPVPSPATTKARKRKTKKPPKRPEATPPPDANATVAAGHATLRAHLREIK
VENADAQFYVCPPPTGATVVQFEQPRRCPTRPEGQNYTEGIAVVFKENIA
PYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINTKG
VCRSTAKYVRNNMETTAFHRDDHETDMELKPAKVATRTSRGWHTTDLKYN
PSRVEAFHRYGTTVNCIVEEVDARSVYPYDEFVLATGDFVYMSPFYGYRE
GSHTEHTSYAADRFKQVDGFYARDLTTKARATSPTTRNLLTTPKFTVAWD
WVPKRPAVCTMTKWQEVDEMLRAEYGGSFRFSSDAISTTFTTNLTEYSLS
RVDLGDCIGRDAREAIDRMFARKYNATHIKVGQPQYYLATGGFLIAYQPL
LSNTLAELYVREYMREQDRKPRNATPAPLREAPSANASVERIKTTSSIEF
ARLQFTYNHIQRHVNDMLGRIAVAWCELQNHELTLWNEARKLNPNAIASA
TVGRRVSARMLGDVMAVSTCVPVAPDNVIVQNSMRVSSRPGTCYSRPLVS
FRYEDQGPLIEGQLGENNELRLTRDALEPCTVGHRRYFIFGGGYVYFEEY
AYSHQLSRADVTTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLLDYT
EVQRRNQLHDLRFADIDTVIRADANAAMFAGLCAFFEGMGDLGRAVGKVV
MGVVGGVVSAVSGVSSFMSNPFGALAVGLLVLAGLVAAFFAFRYVLQLQR
NPMKALYPLTTKELKTSDPGGVGGEGEEGAEGGGFDEAKLAEAREMIRYM
ALVSAMERTEHKARKKGTSALLSSKVTNMVLRKRNKARYSPLHNEDEAGD
EDEL$
Gene Nucleotide Sequence: Sequence Viewer
ATGCGCGGGGGGGGCTTGATTTGCGCGCTGGTCGTGGGGGCGCTGGTGGC
CGCGGTGGCGTCGGCGGCCCCGGCGGCCCCGGCGGCCCCCCGCGCCTCGG
GCGGCGTGGCCGCGACCGTCGCGGCGAACGGGGGTCCCGCCTCCCGGCCG
CCCCCCGTCCCGAGCCCCGCGACCACCAAGGCCCGGAAGCGGAAAACCAA
AAAGCCGCCCAAGCGGCCCGAGGCGACCCCGCCCCCCGACGCCAACGCGA
CCGTCGCCGCCGGCCACGCCACGCTGCGCGCGCACCTGCGGGAAATCAAG
GTCGAGAACGCCGATGCCCAGTTTTACGTGTGCCCGCCCCCGACGGGCGC
CACGGTGGTGCAGTTTGAGCAGCCGCGCCGCTGCCCGACGCGCCCGGAGG
GGCAGAACTACACGGAGGGCATCGCGGTGGTCTTCAAGGAGAACATCGCC
CCGTACAAATTCAAGGCCACCATGTACTACAAAGACGTGACCGTGTCGCA
GGTGTGGTTCGGCCACCGCTACTCCCAGTTTATGGGGATATTCGAGGACC
GCGCCCCCGTTCCCTTCGAGGAGGTGATCGACAAGATTAACACCAAGGGG
GTCTGCCGCTCCACGGCCAAGTACGTGCGGAACAACATGGAGACCACCGC
GTTTCACCGGGACGACCACGAGACCGACATGGAGCTCAAGCCGGCGAAGG
TCGCCACGCGCACGAGCCGGGGGTGGCACACCACCGACCTCAAGTACAAC
CCCTCGCGGGTGGAGGCGTTCCATCGGTACGGCACGACGGTCAACTGCAT
CGTCGAGGAGGTGGACGCGCGGTCGGTGTACCCGTACGATGAGTTTGTGC
TGGCGACGGGCGACTTTGTGTACATGTCCCCGTTTTACGGCTACCGGGAG
GGGTCGCACACCGAGCACACCAGCTACGCCGCCGACCGCTTCAAGCAGGT
CGACGGCTTCTACGCGCGCGACCTCACCACGAAGGCCCGGGCCACGTCGC
CGACGACCCGCAACTTGCTGACGACCCCCAAGTTTACCGTGGCCTGGGAC
TGGGTGCCGAAGCGACCGGCGGTCTGCACCATGACCAAGTGGCAGGAGGT
GGACGAGATGCTCCGCGCCGAGTACGGCGGCTCCTTCCGCTTCTCCTCCG
ACGCCATCTCGACCACCTTCACCACCAACCTGACCGAGTACTCGCTCTCG
CGCGTCGACCTGGGCGACTGCATCGGCCGGGATGCCCGCGAGGCCATCGA
CCGCATGTTTGCGCGCAAGTACAACGCCACGCACATCAAGGTGGGCCAGC
CGCAGTACTACCTGGCCACGGGGGGCTTCCTCATCGCGTACCAGCCCCTC
CTCAGCAACACGCTCGCCGAGCTGTACGTGCGGGAGTACATGCGGGAGCA
GGACCGCAAGCCCCGGAATGCCACGCCCGCGCCACTGCGGGAGGCGCCCA
GCGCCAACGCGTCCGTGGAGCGCATCAAGACCACCTCCTCGATCGAGTTC
GCCCGGCTGCAGTTTACGTATAACCACATACAGCGCCACGTGAATGACAT
GCTGGGGCGCATCGCCGTCGCGTGGTGCGAGCTGCAGAACCACGAGCTGA
CTCTCTGGAACGAGGCCCGCAAGCTCAACCCCAACGCCATCGCCTCCGCC
ACCGTCGGCCGGCGGGTGAGCGCGCGCATGCTCGGAGACGTCATGGCCGT
CTCCACGTGCGTGCCCGTCGCCCCGGACAACGTGATCGTGCAGAACTCGA
TGCGCGTCAGCTCGCGGCCGGGGACGTGCTACAGCCGCCCCCTGGTCAGC
TTTCGGTACGAAGACCAGGGCCCGCTGATCGAGGGGCAGCTGGGCGAGAA
CAACGAGCTGCGCCTCACCCGCGACGCGCTCGAGCCGTGCACCGTGGGCC
ACCGGCGCTACTTCATCTTCGGCGGGGGCTACGTGTACTTCGAGGAGTAC
GCGTACTCTCACCAGCTGAGTCGCGCCGACGTCACCACCGTCAGCACCTT
CATCGACCTGAACATCACCATGCTGGAGGACCACGAGTTTGTGCCCCTGG
AGGTCTACACGCGCCACGAGATCAAGGACAGCGGCCTGCTGGACTACACG
GAGGTCCAGCGCCGCAACCAGCTGCACGACCTGCGCTTTGCCGACATCGA
CACGGTCATCCGCGCCGACGCCAACGCCGCCATGTTCGCGGGGCTGTGCG
CGTTCTTCGAGGGGATGGGGGACTTGGGGCGCGCGGTCGGCAAGGTAGTC
ATGGGAGTAGTGGGGGGCGTGGTGTCGGCCGTCTCGGGCGTGTCCTCCTT
TATGTCCAACCCCTTCGGGGCGCTTGCCGTGGGGCTGCTGGTCCTGGCCG
GCCTGGTCGCGGCCTTCTTCGCCTTCCGCTACGTCCTGCAACTGCAACGC
AATCCCATGAAGGCCCTGTATCCGCTCACCACCAAGGAACTCAAGACTTC
CGACCCCGGGGGCGTGGGCGGGGAGGGGGAGGAAGGCGCGGAGGGGGGCG
GGTTTGACGAGGCCAAGTTGGCCGAGGCCCGAGAAATGATCCGATATATG
GCTTTGGTGTCGGCCATGGAGCGCACGGAACACAAGGCCAGAAAGAAGGG
CACGAGCGCCCTGCTCAGCTCCAAGGTCACCAACATGGTTCTGCGCAAGC
GCAACAAAGCCAGGTACTCTCCGCTCCACAACGAGGACGAGGCCGGAGAC
GAAGACGAGCTCTAA