YASHINA TL, FAVOROV MO; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 410 (abstract no. 94).
Specialty Lab., Santa Monica, CA
TTV is a newly discovered virus, reportedly associated with post-transfusion hepatitis. We investigated the frequency of TTV in several patient groups and the role of the virus as a possible agent for hepatitis of unknown etiology. Material andMETHODS: The following patient groups were tested: acute hepatitis, fulminant hepatic failure (FHF), chronic liver diseases; and control groups. All samples were collected in the Moscow area, Russia. The colorimetric microplate assay was developed for TTV detection. TTV DNA was amplified with biotinylated primers, and the amplicon was detected by use of a TTV-specific capture probe.RESULTS: Among acute hepatitis, TTV DNA was detected in 15% of patients with hepatitis A, in 28% with HBV infection, in 33% with hepatitis C and in 30% of patients with hepatitis nonA-G. Clinical and biochemical features of TTV-positive and TTV-negative patients with hepatitis nonA-G were similar. TTV viremia was determined in 54% cases of FHF known etiology and in 67% of FHF of unknown etiology. The frequency of TTV in patients with chronic liver disease varied from 11-20% in patients with alcohol and cryptogenic chronic hepatitis to 35%, 45% and 57% in chronic HCV, HDV and HBV infection, respectively. In control groups, TTV was found in 28% of patients with acute respiratory infection, 37% with acute diarrhea, and in 38% of professional donors. Analysis of the amplified DNA fragments of 20 TTV infected patients demonstrated variety of TTV genotypes circulating in region.CONCLUSION: In Russia, TTV viremia is frequent in blood donors as well as in patients with acute and chronic liver disease. Although TTV is detectable in patients with acute, chronic and fulminant hepatitis of unknown etiology, the ability of the virus to cause the disease is still unclear.
Publication Types:
Keywords:
- Acute Disease
- Blood Donors
- DNA Viruses
- Hepacivirus
- Hepatitis
- Hepatitis A
- Hepatitis C
- Hepatitis Viruses
- Hepatitis, Chronic
- Hepatitis, Viral, Human
- Humans
- Liver Diseases
- Moscow
- Russia
- Viremia
Other ID:
UI: 102245610
From Meeting Abstracts