SCHMIDT W, WAHNSCHAFFE U, ZIPPEL T, HILBERT S, RIECKEN EO, ULLRICH R; Interscience Conference on Antimicrobial Agents and Chemotherapy.

Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 2000 Sep 17-20; 40: 287.

Free Univ. of Berlin, Med. Clin., Dept. of Gastroenterology and Infectious Diseases, Berlin, Germany

BACKGROUND: The intestinal mucosa is a site of rapid CD4 T cell depletion very early after infection with HIV. We therefore studied the kinetics of T cell repopulation in the mucosal immune system in patients receiving HAART.METHODS: Rectal biopsies and blood samples were simultaneously obtained from 6 HIV-infected patients before and during therapy with HAART. Lymphocytes were isolated and analyzed by flow cytometry. Changes in the T-cell subpopulation (per micro-l blood and per mg biopsy) were evaluated by linear regression.RESULTS: Mucosal CD4 T cells increased from 38+/-17 to 3013+/-351 cells/mg, peripheral blood CD4 T cells from 106+/-44 to 427+/-62. The daily increase in a first phase was 3,9+/-0,9 CD4 T cells/micro-l in the blood and 26,3+/-5,6 CD4 T cells/mg in the mucosa (p<0.03), in a second phase 0,2+/-0,1 CD4 T cells/micro-l and 0,0+/-1,2 CD4 T cells/mg, respectively. Mucosal CD4 T cell changes did not correlate with CD8 T cells or HLA-DR expression. Fig. 1: Increase of CD4 T cells in blood and rectal mucoas in an HIV-infected patient receiving HAART. [table: see text] Conclusion: CD4 T cell repopulation in HIV-infected patients receiving HAART occurs much more rapid in the intestinal mucosa than in the peripheral blood, and can reach normal levels within 100 days. Given the absence of significant CD4 T cell proliferation in the intestinal mucosa this indicates a preferential redistribution of CD4 T cells to this immune compartment the source of which remains to be identified. Supported by grant FKZ 01KI9720 from the German BMBF.KEYWORDS: HAART; Immune reconstitution; Mucosa

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Antiretroviral Therapy, Highly Active
• CD4-Positive T-Lymphocytes
• HIV Infections
• HIV Seropositivity
• Humans
• Intestinal Mucosa
• Intestines
• Lymphocyte Activation
• Lymphocyte Depletion
• T-Lymphocytes
• immunology

Other ID:

• GWAIDS0010276

UI: 102247774

From Meeting Abstracts