The DOMON domains are involved in heme and sugar recognition

Lakshminarayan M. Iyer, Vivek Anantharaman and L. Aravind

SUPPLEMENTARY material S3- Figure 1

MULTIPLE SEQUENCE ALIGNMENT OF THE DOMON DOMAIN

Strand1 Strand 2 Strand3 Strand4 Strand5

Sec Structure EEEE...........................................EEEEEE...............................EEEEEE........eEEEEEEeee...........EEEEEE...

CDH_Pchr_11514399 5 QFTDPTTG --------------------------------FQFTGITDPV---------------------------HDVTYGFVF 8 QSTEFIGEVVAPI 1 ---SKWIGIALG--

NCU05923.1_Ncra_85090051 29 QYTDPVNK --------------------------------ITLSTWRPDP----------------------GSNSGGGDAATYAF 11 DANEYIGLLRCDV 5 ---PGWCGVSHGQS

UM06431.1_Umay_71024697 23 ESSDQASL --------------------------------YADTSCNSDL----------------------------CLRVVYSP SEKRMNMTMSANG 4 ---IGWYAVGTG--

CG-6_Hs_6760015 134 FPTAPPVD 18 --------------------------------YGKPGCNAET---------------------------CDYFLSYRM IGADVEFELSADT ---DGWVAVGFSSD

DOPO_Hs_118791 24 QGSAPRES 1 --------------------------------LPYHIPLDPE---------------------------GSLELSWNV 2 TQEAIHFQLLVRR 1 ---KAGVLFGMSDR

MOXD1_Hsap_17511810 10 WGLLPGTA 7 --------------------------------YPHRTLLDSE---------------------------GKYWLGWSQ 2 SQIAFRLQVRT-- ---AGYVGFGFSPT

SDR2_Mm_6677899 198 CGNKKFCV --------------------------------RSPLNCDPEK---------------------------EPACVFLSF 2 DNQSVMVEMSGPS ---DGYVSFAFSHD

CG8399_Dm_7303004 223 CGQSKNCF 1 --------------------------------FPDGCVATKS---------------------------CTSITVVTV 2 DVFEFEIQSGKGT 1 ---AAYVAVGLSDD

Pram1000004399_Pram_Pram1000004399 27 SDICSSTA 1 --------------------------------QALSGTRLGD---------------------------SPIFVRALV 2 DEVCIEYQLDASV 3 ---ATWFGVGPSRD

TTHERM_00689870_Tthe_89308760 29 FQQNQNDL 1 --------------------------------PLSSSLSLT----------------------------NGITLNFEI 1 GTDIVFMIESTKP ---QGWIGLGFG--

TTHERM_00470970_Tthe_89287333 810 YGDCLPII 2 --------------------------------LVYINNFQISFNN------------------------LGFSLLYGF 2 SKKYVYLNVQGSN ---QGYIALGFG--

TTHERM_00470970_Tthe_89287333 979 TNTYIPDS 1 --------------------------------QVYNKIELPS---------------------------FNTSLYYGF 2 NAQFIYFKLVGML 1 ---QNWFGIGFG--

TTHERM_00470970_Tthe_89287333 16 KTITSLDS 1 --------------------------------QFWNLAQLQVNN-------------------------QQFNFYFGW 2 AQTFVYFRIQGYN ---QGWIALGFGSN

TTHERM_00470970_Tthe_89287333 573 SQDTLTQL 1 --------------------------------YCCTNQIYSI---------------------------PLSTLSYSL 2 SIIYFKYTILTKQ 1 ---DGAFGCGFN--

DDBDRAFT_0205765_Ddis_60469669 15 TFLIGVNG 1 --------------------------------FQNSVILDQN---------------------------SNYNLQWII FNETITIQVSINK ---KAWIGLGWHCQ

AT5G54830_Atha_15239759 165 NNSEPFKA 1 --------------------------------TMFDNCKKLS---------------------------DKYRLRWSL 2 EKGYVDIGLEATT 2 ---LNYMAFGWAKP

RCCS2_09214_Rsp._126734328 7 DFMVRLCA 10 --------------------------------SAKADPARGVVI-------------------------DGVSFRWMF QDGRLVGELSAPT ---PGWIAVGFHAE

ORF16_Ugam_82524010 7 LLIALLCP 5 --------------------------------QERISANATQHLAL------V-KTITI----------KKFTFSYSI DGANLSAKVSFPT ---RGWVAVGFSPK

DP1669_Dpsy_51245521 2 RFFLSVCA 5 --------------------------------GTSVYAGEYPHRIK------T----------------SEMEFSWRL DADRLYVNLSAFT ---KSWVGIGFNPV

Mthe_1462_Mthe_116754758 13 LLLFSGCI 10 SVVSEEWQPDG-----------------------IVGVNEYSHSLK------L-YSPAR-----QGYTGGVMTISWKI DDEYLYMALNGST ---RGWLAIGFEPT

cbdb_A1609_Dsp._73749307 33 SLLFSSCA 21 PAALAEWVADG-----------------------IITVSEYTNSSA------L-N--------------ANFTLFSRT DDQYVYIGIKAKA ---TGWISIGFQPL

TK1382_Tkod_57641317 19 AHLSLGCL 9 QTEQVQWIADG-----------------------KISRGEYTVEKN------V----------------GEMSLHFRV ENDTLYVGISAET ---HGWVAIGFGGG

ebdC_Asp._56476742 2 KAKRVPGG 5 DLDAPIWAGAE---------------------STTFEMFPTPLVMV-----KE-VSPFLALS-EGHGVIKRLDVAALH NGSMIALRLKWAS 11 FVDGVGAMFPVARG

DoleDRAFT_2783_CDes_121540975 9 IATATKEL 5 SVWQSGKAVLE---------------------TAATPLANQPSPYI-----KG-VYDETK-----IGAVKKITIKAVH NGKDIVFYCEWES 12 FPDGVALLFPFKDI

PAE3613_Pyae_18314195 34 TVKYIAGP 3 DPFSNLWPA-----------------------PVEVPLTSQTLVY-----------PLPA-----ATETRSIGVSAVY NGTHIAFLLTWSD 13 FPDAVAVQFPVSKA

APE_1297_Aper_14601316 38 NAVYVDGA 3 DPTDSFWQQME---------------------KAEISLVSQNIVY-----------PMTG-----YEDVRTLRVAAAV 1 SEGILAIYLEWED 13 YPDKVAVQFPLSSD

rrnAC1203_Hmar_55378005 37 PVSEVSTA 3 EPNADGWDEVP---------------------ASDVPLSSAPSSV-----------PNAD-----DTSVETVDVQAAR TDERLFVRLEWHD 12 FADAVAVQFPTNTS

ddhC_Rsul_38372235 44 VIKIPDGI 6 DPDDIIWARVP---------------------EFRVEMVMAPPVH-----------PSVGLRYRDEYPEQDLVVQLAR TSERFYVRLRWVD 11 FRDGAAIEFSESDD

clrC_Idec_38372174 40 RVLSVAGG 3 SPQAAVWKKAP---------------------TTQVTLLTAFPGH----------ISIVG-----TAATQKLAAQAVR ASGRLFVRLAWSD 12 FLDGAAVEFPVNGK

serC_Tsel_38372504 40 QVLSVKGG 3 SPQAAVWKKAP---------------------TGQVALQTAFPGH----------ASIVG-----TALTQQMTAQAVR AGDRLFVRLAWRD 12 FVDGAAVQFPVNGK

AT3G62370_Atha_15228739 38 LADFRPGI 3 DGHNDDWKD-----------------------IDGSEFPLRPAL-----------DPDSDH----EYDAGKMTVKALH -DGRDIYFLLEID 11 KCPSVALMFQIGDQ

cbsA_Ssol_15899519 47 PVYKVVGN 5 PGSASYWSQ-------------------------IPWINISLTAN----------IPMAPTS----GLTHYLLVKAVW NGSWIIILERWYA 112 YPDRAAIMWYMGSV

cbsA_Msed_42516539 39 VAYKISGS 5 PGTESFWSK-------------------------IPWDNISLTAN----------IPQAPTS----GLTHYVLVKTAW NGTDLMVLMSWPA 112 YPDRAAIMWYMGSV

CmaqDRAFT_1308_Cmaq_126353324 45 MAYKVVGS 5 PGSEAFWSK-------------------------IPWFNVSLVPNL--STQML-PIPANVPT---SGEVPYIMVKAAW NGSYIFILVKWPD 88 YPDRVAIMWYMGSE

SIAM614_01941_Sagg_118592944 277 LFVAEGAT 6 KLDDAAWQS-------------------------APPVFIRTQQG----------SNLNGT-----GES-TVEVRAVQ VGDKIAFAFRWED 32 YEDKFSVAFSTTDT

blr7997_Bjap_27383108 237 LVVARMSD 6 AMGDPAWSR-------------------------ARPVFIRTQQG----------ANLGGS-----GES-LVEVRALH DGQKIYFAFRWED 32 YEDKLAVIFSDNPS

PTD2_04281_Ptun_88857750 180 VQPIPLAT 6 QGNEASWQY-------------------------APAIIIQTHGG----------ANFVNG-------QTKVEIKALS NSEEVYFLFRWAD 32 YEDKFAVMLSPSCE

HCH_03667_Hche_83646397 190 VAPLAEKV 6 APDEDAWAN-------------------------AAPVTVTTYQG----------NDYDAV--------IPVEVRALA NPYTIYFSISWPD 32 YEDKLAVMLSKGGG

amb1398_Mmag_83310497 256 KTVLFYPG -QTAMEWILTGTDHGGTRAF-KKGDRCFECHSNETADMGAKMVSGA--KAEKT-PIPGKRA-------AIPLSVKAAY DADNLYMRFEFPA 17 NEVKVAMMIDNGK-

nirB_Mgry_144897392 40 KTVLFYPG -QTSYEWIQTGTDHGGARSFLKKGDNCAGCHSEETADMGKKMVSGQ--KAEAT-PIPGKRA-------SVPLSIKAAY DADTLYMRFSFPA 17 NEIKLAMMFDGGK-

nirTB_Cpsy_71278993 292 DIGVFYPG -TASIEWIL-GRKHGGKRAF-TKGDRCIECHGEEIADIGNNIVTGESEKKLEPNVIPGKRG-------HIDVTIDSTY DAENLYLRFSWAD 17 NPMKLAFMLATDD-

nirB_Pstu_115239 36 DVTLFYPG -VSPVEWITKGTEHGGARAL-KKGETCAGCHSEEASDMGEKMASGK---KLEPSPIAGKAP-------FINAKVQAAN DGENLYLRFTWKQ 12 NPVKIAYMLEGGSK

Magn03010753_Mmag_23016307 29 EVVLFYPG -QSSLEWVLSG-DHGGAPKV-KNGKACKECHLGEEKDMGATLVSGA---KNEPAPMAGKPA-------FIPATVRVAQ ENGRLLVRLEFKE 11 FATKVTMMLDDGT-

Hhal_2278_Hhal_121999057 349 VDLEGGLF -DSVRLWRAASA-------------------LLDVRLDPEQPLASD-PGRNVYIPPRGELE--------RTLRVGAVY NDDEIQIRYEFAT 38 YEDRISMLLDDGD-

PBPRB0283_Ppro_54301963 34 LISYKTTQ 6 -MPEKEWEK-------------------------ATPLTMLLDYM----PYKP--DQYEGM------LKTQYSIKSLH DDQNIYFFVQWDD 33 YEDKASIFWAINA-

P3TCK_18604_Ppro_90414275 29 LESTKTSS 6 -QVDAVWND-------------------------IKAAKIKLGKL----PYKP-NNGYEGI------KKTTVEIKSLY DKEFIYFLISYKD 33 YEDKFAVFWDINA-

Sfum_0443_Sfum_116747891 31 ETLAAAKV 6 -LDDPVWQK-------------------------AKPLDIPFAGK-----------EKFAG------KKANVITQAVY TDDEIFFLFKWKD 22 DEDRISLLFEINRI

FG07921.1_Gzea_46127087 18 PSVSV--- -PACPKYG--------------------------TIKFSKSVPD-----GDPF-P---------------RTEVDLCY TDTALSLQFTAFD 15 IWAYEVVEAFIYK-

DDBDRAFT_0183821_Ddis_66802540 24 ETLNIYRC 3 --STNQWSNA-----------------------TSVTDFTLTSN-----YGLA-IQ--------------QTFASICY DDQYINIKAYCVD 13 LFNADVFETFLSY-

TTHERM_01004980_Tthe_146162880 33 NQLVVAAC 8 EQQDRIVGF--------------------------IPYFYYQNG-----TGVS-DI--------------STTVQICH 1 NFEIIDITWNVAD 13 LYNQDVVEVFLGT-

RcasDRAFT_0067_Rcas_118063192 8 PHYHVVRV 8 DPFAWDWTQM-----------------------PAIMPFVLADG-----SGPA-TQ--------------QTMARLGY DSDCLYVRFDCED 13 IYNEEVVELFISP-

Acid_6151_Susi_116625206 14 DVLESAYA 8 DPNRAEWAKA-----------------------PRVSAGRDYLD-----HPVA-GR--------------PIEIRSRW TKQNLYLLYICPY 17 LWNWEVAEAFIGS-

SO2192_Sone_24373747 47 LYAYPLAG 6 --KLNEWQEYQHR--------------------FIQYGEANQIMR----ADPSAPLT-------------LSFTHMVG 1 YDGYLYAFFQVVD ---PNVVFRGKNAL

CPS_3981_Cpsy_71279576 84 LYGYKLTE 6 --LPNDWPDFNQR--------------------THFYNQQQQLF-------ANEPLS-------------LNFKASVG 1 YNNYLYLFFQVVD ---NRLIFRGENTR

PBPRB0757_Ppro_54302436 47 LYAYDLQN 6 --KLSDWHDYDSQ--------------------MVNYGRDYVQFS----RRPYSASNS------------INFKHMVG 1 YQGYLYAVFEVND ---KSPIMRRKNSA

DP0752_Dpsy_51244604 74 LYLYPLTG 6 --LQDDWQEQPGS--------------------FQLFGKEHLRSA----TD-S-SSA-------------FYFRHRSG 1 WQDYLYAFFEVHD ---ATVVYRPPQSL

xynA_Tmar_14719787 2 VATAKYGT 5 -EIDEIWN-------------------------TTEEIETKAVAM----GSLD-KNA-------------TAKVRVLW DENYLYVLAIVKD 11 EQDSVEIFIDENNH

xyn10B_Xpac_5880612 877 DVVEAAQA 5 -QVDDAWA-------------------------DAEVVSTGKTV-----EGGA-DGA-------------TAQVRTLW 1 GDDTLYVLAEVTD 11 NQDSVELFLDLGNT

xynX_Cthe_586269 536 SAKALEGS 5 -NVDSSWK-------------------------LVKPLYANTYV------EGT-VGA-------------TATVKSMW DTKNLYLLVQVSD 4 SNDGIEIFVDKNDN

xynC_Tsp._4894694 662 TSTISEGE 5 -KMDDSYL-------------------------MSKPIEIYDE---------E-GNV-------------KATIRAIW KGSTIYVYGEVQD 5 AEDGVAIFINPNNE

BBFL7_00085_Fbac_89890737 37 RATRISNP 6 -PFEQVWDRV----------------------TTGGNFIMAQPN-----NGNA-ERDTH-----------KTEFKVAY DDDAIYVAGFMYD 18 QADLFGFFINTY--

MED134_14592_Csp._86132959 23 KAHRTGEA 6 -LTEAVWKAQ----------------------LETKTFTQKAPN-----AGQP-SLR-------------ATEIAVLY DDNYLYVGAVLNT 17 NADYFGVQIDPF--

ATW7_03127_Abac_119468755 37 SLARINQN 6 -LDEAQWQKA----------------------TYVPLLVQDEPN-----ERGA-PPV-------------KTDAYMYE DGTNLYVGFIAYD 17 QDDTVILVIDTF--

RB2501_14839_Rbif_88805626 39 EAVALDEE 7 -LGDPVWQAI----------------------PATEGLRQIRPN-----YGQP-VSE-------------RTAVRVAY SRSTFYLSVVCYD 18 DEDSFLFIIDTY--

M23134_01584_Mmar_124008041 43 KATQITAN 6 -LNESVWAKA----------------------KKYQAFTQVEPN-----QGQV-SDF-------------ITEVKVLY NDQYLYIGAFCKD 19 SGDLFGVVIDAF--

ALPR1_00920_Asp._126645907 29 IAVKAKQG 6 -LTEDIWLDPT---------------------GWNTDFMQYFPS-----DTSL-SQT-------------PTRVKIAF DEHNLYLAAVMEN 18 QNDGITFVFDTF--

GB2207_03065_Mgam_90417401 9 CIPTVSAG 6 -LDEEEWAGA----------------------EIYTDFVTVEPL-----TGDS-AKY-------------GTQVRVIT NSDGIFVGFSNYQ 17 EADRNIVSIDFD--

EBAC31A08_Umar_9971878 14 SVLIWSNS 6 -LDEPEWNNA----------------------FEITDFYETSPF-----TLKK-NEL-------------LTTALIFS NKDGIYVGFKNFQ 17 LSDKNSINIDFD--

consensus/85% ......................................................................................h.h...........l.h.h...s...........h.h.....

consensus/80% ..................................................h...................................h.h.......s.p.lhh.h.h.s...........h.h.....

Strand6 Strand7

Sec Structure ..........................................................................................EEEEEEe...EE..........EEEEEE...........................

CDH_Pchr_11514399 -----------------GAM-------------------------------------------------------------------NNDLLLVAWA-NGNQ----------IVSSTRW----------------ATGYVQPTAY

NCU05923.1_Ncra_85090051 -----------------GQM-------------------------------------------------------------------TQSLLLMAWA-SKGQ----------VFTSFRY----------------ASGYNVPGLY

UM06431.1_Umay_71024697 -----------------KQM------------------------------------------------------------------DGSNMMIGWVD-TAGK----------VVMSQRT----------------ASGHTSPTTS

CG-6_Hs_6760015 -----------------KKM------------------------------------------------------------------GGDDVMACVHD-DNGR----------VRIQHFY----------------NVGQWAKEIQ

DOPO_Hs_118791 -----------------GEL------------------------------------------------------------------ENADLVVLWTD-GDT-----------AYFADAW----------------SDQKGQIHLD

MOXD1_Hsap_17511810 -----------------GAM------------------------------------------------------------------ASADIVVGGVA-HGR-----------PYLQDYF----------------TNANRELKKD

SDR2_Mm_6677899 -----------------QWM------------------------------------------------------------------GDDDAYLCIRE-DQT-----------VDIQPSY----------------LTGRSYPVMD

CG8399_Dm_7303004 -----------------AKM------------------------------------------------------------------GDDLTTECVPE-NGR-----------VNLYSSLTS--------------ASPYSAVRSN

Pram1000004399_Pram_Pram1000004399 -----------------SNM------------------------------------------------------------------VSSPATNVMMFFKDA-----------GVPTSYL----------------LGGYVNSDVT

TTHERM_00689870_Tthe_89308760 -----------------ATM------------------------------------------------------------------TNTDMAIFFANMNGQ-----------PAVQDAY----------------STNTVRPPFD

TTHERM_00470970_Tthe_89287333 -----------------SHS------------------------------------------------------------------TFMDIIMIQNSISNN-----------TLVTDMF----------------SQGQIFSQQD

TTHERM_00470970_Tthe_89287333 -----------------NSM------------------------------------------------------------------TSTDMIITRFAKQSAQGSVQG-----FVVDDRF----------------SDGQSMPGLD

TTHERM_00470970_Tthe_89287333 L----------------SQM---------------------------------------------------------------------DMIVLRLNSLR------------VVAEDRFS---------------LSDSQVPDLN

TTHERM_00470970_Tthe_89287333 -----------------KNS------------------------------------------------------------------VNTDMMIFRFKT--------------LDVQDYF----------------SYGEVIPELD

DDBDRAFT_0205765_Ddis_60469669 GCQKD------------GPM------------------------------------------------------------------ENADYSIATFDENGQ-----------GYVWDCYQEN-------------DPTSQPQNDT

AT5G54830_Atha_15239759 NSTS-------------NLM------------------------------------------------------------------LNADVVVTGIREDGF-----------PFADDFY----------------ITESSVCSVK

RCCS2_09214_Rsp._126734328 -------------TGLQGTY----------------------------------------------------------------------FVIASV-ADDV-----------HRIEEHF-----------------AVVPDHIPI

ORF16_Ugam_82524010 -----------------KRM------------------------------------------------------------------LGANIIIGSISPEGPDKG--------IRIDDQY----------------GIAPTRHKPD

DP1669_Dpsy_51245521 -----------------KKM------------------------------------------------------------------KGANFILGHV-ENGV-----------LKLVDEV----------------GTGLTRHQND

Mthe_1462_Mthe_116754758 -----------------EWM------------------------------------------------------------------RDADMVLGV--VDGN-----------ARVLDEYS---------------TGNYGPHIED

cbdb_A1609_Dsp._73749307 PA---------------KGH------------------------------------------------------------------TSADFALGGV-SSGE-----------AYIYDLW----------------GLNKEEHSLD

TK1382_Tkod_57641317 -----------------PGM------------------------------------------------------------------RNTDIVIAYVLPNGT-----------GEISDSYS---------------TGFSGPHNPD

ebdC_Asp._56476742 --------------AQAVTMGA----------------------------------------------------------------TGRPVNAWYWKANANEP---------MEIVAE-------------------GFSAVRRM

DoleDRAFT_2783_CDes_121540975 DK------------TPINEMGT----------------------------------------------------------------QDYPTNAWYWRPDFEEKP--------KNQVSH-------------------GLSTSLYT

PAE3613_Pyae_18314195 Q-------------LPYICMGT----------------------------------------------------------------VDNPVNIIYWRAGFGA----------ENLVAG------------------AGYGLNPQQ

APE_1297_Aper_14601316 S-------------LPYICMGT----------------------------------------------------------------AEQPVSIVLWSASGST----------ETLIAG------------------SAYGMSPEH

rrnAC1203_Hmar_55378005 S-------------RPPIAMGG----------------------------------------------------------------PDNRVNVWYWSGATGT----------QELLAG-------------------GAGSTTAF

ddhC_Rsul_38372235 --------------SVSYMMGTD---------------------------------------------------------------AESPVNIWYWHPDGDR---------VESLAAG-------------------SPGSLTRL

clrC_Idec_38372174 V-------------ATLPFMGD----------------------------------------------------------------PVNVVNVWHWRADGRT----------LNLLAK-------------------GFGTSTPV

serC_Tsel_38372504 D-------------TTLAFMGD----------------------------------------------------------------PDNPVNVWHWRADGRT----------RNLVAK-------------------GFGTATPV

AT3G62370_Atha_15228739 --------------ATYHNMGGCKEGTDSCTSKA---------------------------------------------------CRGFEVDIMHFSIGNAIP---------GRLYGG-------------------NPIDNGEG

cbsA_Ssol_15899519 IP------------PTKDGMNIGGKVPGQAFD---------------GVTFNDTGGSLVQ--------------------------PGGAANIWMWVSGATW----------NNATYDPAFKVNLWQNTS-------LTGLPYID

cbsA_Msed_42516539 IP------------PTSDCMNIGGKVPGQEFD---------------GFTLKDAGGSLQQ--------------------------QGGAANIWMWVSGATW----------NNKTYDPAFAANLWNNES-------LTGLPYTD

CmaqDRAFT_1308_Cmaq_126353324 Q-------------SPSDCMNIGAGHPNFVVKSELGQLMI---PYTGSSTIQYTGGSLS----------------------------SGAADIWEWISGATY----------PEGQNFNYFTNVTWDVENGI----DEAAASVFS

SIAM614_01941_Sagg_118592944 FGG-----------GGATHMGPKPLGDKPAAFNSR------------GLHYTT---------------------------------DGSLVDVWQWKASRGG----------MLGRVDDMHFSTPVEPNEAQAAGKSRYSAGYNP

blr7997_Bjap_27383108 LGG-----------AGATDLGANPLPGKPMPINGR------------GFHYTT---------------------------------DASYIDMWQWKASRGG----------MLGRVDSQYIGPPYAPTLDEQNYDARYQGGYWN

PTD2_04281_Ptun_88857750 HGG-----------DGTTHLGPEPLAGKPKNFHGK------------GYHASL---------------------------------DGKPRDLWHWKALRTN----------DMFLADDNHIGAPLLAQ----SGSRRYSAGYFV

HCH_03667_Hche_83646397 FGG-----------DNSVHLGGRPLEGYPASRSGR------------GYHYTT---------------------------------DGSIRDVWHWKAVRGG----------DMAVLDDDHFGKPEAAC----AFCPRYKAGYQP

amb1398_Mmag_83310497 VDMAARSGCWTSCHSDARDMPTAPAAGSLGTAKGIDTSH------GYVTKYVPESRTQFDTTK-----RDNWDKVKPQAELDALLAGGTFLDLTRWKSSGVS--------EQGYVLAER----------------------VLKP

nirB_Mgry_144897392 VDKAAQSGCWASCHNDARDMPSAPKKDALGAAKGIDTSH------GYVTKYLPESRTAISLKDEP---RGGWDKVKPQAELDALLKDGTFLDLARFKSGKGG------VSEDGYVLAER----------------------VLKE

nirTB_Cpsy_71278993 VEYADRAGCWGTCHADANSMPFAPDADTLKGSDLAKRLKFDTGSGTGVTKYLKESRTKLELKGRGGKALGGWDKLKDQSDIDAAQKSNQFMDVIRYKSGTKE-------VEDGQILAER----------------------DMHK

nirB_Pstu_115239 VELAEAGGCWGSCHGDARTMPGAA---------------------DTKTKYVK----------------------------DGSLANGVYYDLNQWRSGENK-------AFDGYVATER----------------------VMEG

Magn03010753_Mmag_23016307 LPEATQAGCWGTCHEDAAKMPAGGT--------------------GERTKYLNRSRAKPSRQGAG-----DELVAAAELEKMRASGQVMEFWQARLKPGMAAV------AASGTIFDKR----------------------METK

Hhal_2278_Hhal_121999057 LGLD-RYGGFMTAHEGMRGLTGAAESEEVEAHPHLGETLGR----SDVRKYIPQSREDGDNGGAD------WQDVRDEAELEAMRERGEFLDLWQWRAHRSHPLGY---ADNGYVLEYRHS--------------SEGRGMFTDN

PBPRB0283_Ppro_54301963 KGFS-KKGCDIACHLADEEGKVAGIEQKSA-----------------GRKYTRK--------------------------------DGETIDMWHWKGVRTG----------LTDQFDDQFVDAT------KDPGAN-SNWGRKG

P3TCK_18604_Ppro_90414275 DGFA-EKGCNAACHRSKDGKIAGRDDKNP------------------ARKFT-K--------------------------------EGQYLDMWHWKGVRTG----------AHGQLDDQYVDSN------TDPKAN-KGWGRKG

Sfum_0443_Sfum_116747891 NNFA-TKGCAVTCHGAAGAAVKDF------------------------KFATAS--------------------------------AGEKGDLWHWKAARSD----------PYKSADDGWLTAA------G------EKTGRKD

FG07921.1_Gzea_46127087 ------------GTDDPQTYL-----------------------------------------------------------------EYEINPNNVTYQAFVYNPSKVRAEGAPFDHFFI-------------------SDPEGDG

DDBDRAFT_0183821_Ddis_66802540 ----------GPADQVAVNYL-----------------------------------------------------------------EVELSPYGVLFVSLVNNPNDQ--CDGIQDTLVD-----------------------CGQ

TTHERM_01004980_Tthe_146162880 ------------PENYLTEYL-----------------------------------------------------------------EVEVNPQGALFAANITNTNNS--CSGISDSLIP-----------------------CNE

RcasDRAFT_0067_Rcas_118063192 ------------GAETPVAYF-----------------------------------------------------------------EFEISPNGVFFDARIGNPHED--RATLRVDLAW-----------------------NAD

Acid_6151_Susi_116625206 ------------DYAHIARYK-----------------------------------------------------------------EFQVSPQGEWVDLDIDRENQK-----GEQGMKW-----------------------DSG

SO2192_Sone_24373747 SV-----DRNDHLSIATLAPDN-----------------------------------------------------------------KFKRYIVATTQP-------------GWISAFELPA------------------NPKQT

CPS_3981_Cpsy_71279576 SI-----TNNDHLALSFSDTNN-----------------------------------------------------------------KLNRFIISNKKA-------------GWISAFALTD-------------------DETA

PBPRB0757_Ppro_54302436 RV-----DRSDHLIIAMTSPNG-----------------------------------------------------------------EFQRYVISPYES-------------GWVNAFRITKP--ADNNQDSATSYNTSMIDPAS

DP0752_Dpsy_51244604 RL-----DTSDFLEINTEDANG-----------------------------------------------------------------LSRRYLITTSKP-------------GWVNGFLMA------------------ESGAYA

xynA_Tmar_14719787 KT--------------GYYE--------------------------------------------------------------------DDDAQFRVNYM-------------NEQTFGTG---------------------GSPA

xyn10B_Xpac_5880612 KP--------------AAYG--------------------------------------------------------------------PNVSQMRISAD-------------NVTSFGTG------------------DAAAQAA

xynX_Cthe_586269 KS--------------TSYE--------------------------------------------------------------------TDDEHYTIKSD-------------GTGSS-------------------------DI-

xynC_Tsp._4894694 RT--------------PYLQ--------------------------------------------------------------------PDDTYVVLWTN-------------WKSEV-------------------------NRK

BBFL7_00085_Fbac_89890737 ----------------NNQI--------------------------------------------------------------------NQTRFYATSAN-------------ALGDAIAE----------------------GNN

MED134_14592_Csp._86132959 ----------------GQTR--------------------------------------------------------------------EGYDFTVTAAG-------------VQFDSKIS----------------------GNT

ATW7_03127_Abac_119468755 ----------------NDER--------------------------------------------------------------------SGYEFFVNPLG-------------AQGDLRMS----------------------DTD

RB2501_14839_Rbif_88805626 ----------------NDRQ--------------------------------------------------------------------NGFLFGTNPQG-------------MEYDAQIN-------NEGKGNFNANRQQGGVIG

M23134_01584_Mmar_124008041 ----------------RDER--------------------------------------------------------------------NAISFQTNPYG-------------ARRDMQVF----------------------DSN

ALPR1_00920_Asp._126645907 ----------------NDKT--------------------------------------------------------------------NAFQFGVNPYG-------------VQREGLLA----------------------NGG

GB2207_03065_Mgam_90417401 ----------------STAL--------------------------------------------------------------------AGYDFTVGSAN-------------SQQDGIVT----------------------PGD

EBAC31A08_Umar_9971878 ----------------GDGN--------------------------------------------------------------------KAFILAINLGN-------------SLFDAIKT----------------------QAG

consensus/85% ...............................................................................................h..s...............................................

consensus/80% ............................................................................................hh.h..s...............................................

Strand8 Strand9 Strand 10 <----Strand 11------------>

Sec Structure eeEEEEEEEEE.......eEEEEEEEEe..... ......... .eEEEEEEEEE.........................EE.......EEE.----------EEEE

CDH_Pchr_11514399 4 TLTTLPETTIN STHWKWVF-RCQGCTEW NNGGGIDVT SQGVLAWAFSNVAVDDPSD--------PQSTFSEHTD------FGFF----------GIDYS 174\CLASSICAL

NCU05923.1_Ncra_85090051 3 ATLTQISATV- NSTQFELIYRCQDCFAW 1 QGGSKGSVS 23 DKAIPGFHDNGFGQYG-----------APLEKVPHTS-------YSA----------WASLA 231|

UM06431.1_Umay_71024697 5 ATMEPKHSFSN SSGTVWTWSFPMSG--- --SDATPSA -STPFIWASNKADNPSSS---------TTASIKRHTA------FGSI----------TLDLT 182|DOMON

CG-6_Hs_6760015 1 NPARDEEGVFE NNRVTCRFKRPVNVPRD ETIVDLHLS -WYYLFAWGPAI----------------QGSITRHDI------DSPP----------ASERV 304|

DOPO_Hs_118791 2 QDYQLLQVQRT PEGLTLLFKRPFGTCD- PKDYLIEDG -TVHLVYGILEEPF---------------RSLEAING------SGLQ---------MGLQRV 181|

MOXD1_Hsap_17511810 2 QDYHLEYAMEN STHTIIEFTRELHTCD- INDKSITDS -TVRVIWAYHHEDAG-------------EAGPKYHDS---------N---------RGTKSL 169|

SDR2_Mm_6677899 2 GTLEDMAWRLA DGVIQCSFRRNITLPEA KNRFVLNES -YYIFFAEGPSH----------------DGRIFRHSQ------QPLI----------TYEKY 352|

CG8399_Dm_7303004 3 NSARLLDASIV DGVIYCRVQRDAVTNVQ GRTFDLRNG -KYHLLVASGSSL--------------KENSVGYHDI------GRLP----------SAQPI 384|

Pram1000004399_Pram_Pram1000004399 3 DQSAFVVGETS TTSMSFSYQRTLAAATT SEVALSASG -ATDFIWAYGT-----------------SWPISTHRS-------GTN----------GAATF 185|

TTHERM_00689870_Tthe_89308760 2 QDWTLLGSSIT SNSFQMKAKRALNTGDT 1 NDIILQQGQ -TYNFCIAWSS-----------------SQTFGYHDS------YYSY----------SITLT 181|

TTHERM_00470970_Tthe_89287333 2 TNVAYLSGGYN NGFWNITVLRPIISTDS QDLNISLTT 23 KSQFYYAKAQGGQVNYTNTY-------IPDSAQVYNK------IELP----------SFNTS 1001|

TTHERM_00470970_Tthe_89287333 2 QDVVMIRSTNI 5 STNSTVEFVRKLITTDP 8 YNTNTNQTN -PIDLIFAQGID---------------SDFTQAGHTS-----HLLMR----------GVILN 1155|

TTHERM_00470970_Tthe_89287333 3 KDTFLIKGERN 1 TGYWDATFKRRVTSVD- PKKVMLDTS -QRYITILLGQSN-------------DEEFT-QLRPSSQECTGCDYF----------RVLYQ 181|

TTHERM_00470970_Tthe_89287333 2 QNLFVTQLTG- QQGFEVVFARNLGLDSS 3 QDADLTNYS -GPMICYLGSTP----------------DLSPNNRQM------FYNP----------QVVFQ 727|

DDBDRAFT_0205765_Ddis_60469669 6 DNIISFSGYQT DQYSIMMFTKKLSTGDT 1 GD-HILVNG -PVDFVWAHGQ-----------------DNSFGFHGVGN----AGRT----------TVNLA 182|

AT5G54830_Atha_15239759 23 NNTKLVYGHRI DGVSFVRYRRPLNDSDN 1 FDFPVNST- ESLTVIWALGVIKPPDVIN--------PYYLPVNHGGV-ESENFGHF----------SLNLS 362|

RCCS2_09214_Rsp._126734328 6 NTARLHAGLFQ DGVSSMSFSIAATSPFG ARVALQSGR -HVNLMLAWSHEA----------------DFDH-HSA------WRKH----------IAISL 173|

ORF16_Ugam_82524010 6 NNIIDGSAIEE NGTTTIFFTIPLDSRDS ADTPLVPGT -ETTVIFAAGKQE----------------DLSAKHSW------TAK-----------IKMKL 181|

DP1669_Dpsy_51245521 6 MDVTAIAGSLV NGRTNIEFSLPLQSGDI NDSEINSRG -ENIILLAHGWKMN---------------NLRKKHSY------RAKF----------KVNLQ 169|

Mthe_1462_Mthe_116754758 6 DDILEYGGKSY GTHTVAEFRRRLDTGDR FDKVLRLGK -DVSIIWAMSDNT----------------DPSVKHNIA------YGE----------GLIYL 203|

cbdb_A1609_Dsp._73749307 6 SSILEYGGTES GGYTILEFKRLLTTGDT YDQNIVHGS --NNILWAYSDED----------------GFAAMHIA-------EGT----------GKINI 225|

TK1382_Tkod_57641317 6 TDILSYGGRED ENGTVVEFSRPLNTGDG YDYVIPTEE -SFRIIWAYGPTD----------------DFQSMHIK------AGH-----------IYVTL 199/

ebdC_Asp._56476742 4 GSDLKAVAQHR NGEWNVILCRSMATGD- GLAKLQAG- GSSKIAFAVWSGG--------------NAERSGRKSY-----SGEF-----------VDFEI 212\EDHg

DoleDRAFT_2783_CDes_121540975 1 KSSISSYSRWA NGKWCVVMARPMKATQP 2 ETVDLMPG- KAIGFGIAVWEGS--------------NGERGGIKAF-----SKEW-----------RELVI 219|

PAE3613_Pyae_18314195 13 VELLPASAQVI 7 DGKWYVVIIRPLGSVHP LMASLA--- EGFSAAFATWDGA--------------KGEIGGLKA------TSGW-----------VTFQL 248|

APE_1297_Aper_14601316 13 IELVPPEAQVW 7 DGKWMVVLYRPTGSIHE LVPTLEPG- SETSVAFAVWQGS--------------KAEVGGKKS------TSAW-----------FTMRL 257|

rrnAC1203_Hmar_55378005 1 NPTVQANASHS 4 NATWTVVYSRQLDAPSE NRTDLRTD- GDLDVAFAVWNGS--------------NGERSGQKA------VSEW-----------HYYPF 238|

ddhC_Rsul_38372235 1 RQPVTGASEYR 6 DSQWIVVMSRPLASEGD HQVSFER-- DTIPVAFALWQGA--------------DAQRDGLKL------VSLN-----------WIFAR 254|

clrC_Idec_38372174 1 TEDLRSASVRT GDGWEVVLSRPLRVKAE EGANLQGR- RTMPIGFAAWDGE--------------NQERDGLKA------VTME-----------WWQLR 238|

serC_Tsel_38372504 1 AEGLRSTATRT RDGWEVVISRPLRVKAE EGADLQGR- RTMPIAFAAWDGE--------------NQERDGLKA------VTME-----------WWQLN 238|

AT3G62370_Atha_15228739 36 DWHGAWWHSSF 18 KGTYYFEFSRPLRTMDR 2 QDVQFTLG- SPAKMSVAFWYPVDS------------KPWHGSGHYT-----INCD-----------WTPLD 303/

cbsA_Ssol_15899519 38 LFFIWTGATYQ NGYWTVEFARPLAVPSA 3 WMPNITVG- KTYYVAFAVWQGK--------------LGETLFDKS-------ITS-----------NFLTL 419\CbsA/cytochrome

cbsA_Msed_42516539 38 LFFVKTGATYS NGFWTVEYVRPLQVPQA 3 WMPNFTVG- NTYYVAFAVWQGK--------------LGETLFDKS-------ITS-----------GFLQF 411|b 558/556L

CmaqDRAFT_1308_Cmaq_126353324 36 YFSNYAGAKYE DGYWIVEFVRPLKAP-- 2 YTVNLTPG- QTYDVAFGVWLGK--------------AGETSWDKS-------ISA-----------SFIPL 408/

SIAM614_01941_Sagg_118592944 75 VGTVIPGVLIQ 17 DGYWTLEVVRDMDTGQS QDLAMKDG- 1 FMWVSVFDHNQ---------------------TRHT------RHSR-----------PVRLT 618\Hajella HCH_03667

blr7997_Bjap_27383108 74 VGTILPGVIIA 17 NGHWTLELTRNMKSDGR YDKAFVPA- 3 YMWVAVFDHAQ---------------------TRHA------SHNR-----------PVRVV 579|

PTD2_04281_Ptun_88857750 58 VGSVLPSVLYR 18 DGFWTLEMVRKREIRSP NDVPLVQG- 1 CMWVSAFDASQ---------------------IAHT------RHDR-----------PVQLV 500|

HCH_03667_Hche_83646397 55 VGDGLPSVLWM 17 DGRWYLELARARETGSP FDIPIEDG- 1 FMWVAPFDHAQ---------------------TRHA------YHHR-----------PLRLR 505/

amb1398_Mmag_83310497 GNDVAYSGKKE GDKWVVTMVRRLKATQP GEANLVAG- QSYSVGFAVHD----------------DFTSGRFHH-------VSI-----------DLKLG 540\NIRT-C terminal

nirB_Mgry_144897392 NAGATFTGKKD GDQWVVTMTRKLKSGQP GDVALEDG- KAYTVGFAVHD----------------DFTAGRFHH-------VSV-----------DMRLG 329|

nirTB_Cpsy_71278993 GVGAAVEASLK NGTWSVVVKRKLKSDKA GDVSIETG- KVYNFGFAIHD----------------DYSSARYHH-------VSF-----------GYKLA 590|

nirB_Pstu_115239 1 QALVDAQGKLD GDTWTVVFTRKFAGGE- GDVTLAPG- NLYNFGFAIHD----------------DSATGRYHH-------VSL-----------GYSLG 279|

Magn03010753_Mmag_23016307 PSAVTAEASSN GDAWTVTLSRPLSAPAP -YKSIETG- KTYTVGFAIHA----------------GHTARRFHY-------VSL-----------EHSLA 293|

Hhal_2278_Hhal_121999057 70 SRGAIRAAGGY 1 DGAWRVRLTRSLEAPEP 1 DSHTLEPG- GVYDVAFAVHE----------------G-VGQRWHR-------VSL-----------PQTLA 721|

PBPRB0283_Ppro_54301963 47 PGDKLPGIVVS 16 DGKWTLEFQRKLVTTGE 4 EDVQFSDLS 3 YFGVSVFDNSQ---------------------INHI-------YHE-----------GALKL 354|

P3TCK_18604_Ppro_90414275 44 ASNRIPGLTGA 16 DGVWTLEMKRKLVTTGS 4 QDVQFNDMN 3 PFGVAVFDNSQ---------------------INHV-------FHR-----------GVMNL 345|

Sfum_0443_Sfum_116747891 49 ANDTLTYRMPK 16 DGGWTVMLSRKLDTGND DDVAFNT-K 3 SFALALFDDSM-------------------DYDSYD-------SEAL----------VLEFG 324/

FG07921.1_Gzea_46127087 -FTAETQLNKR AKKW-VSKAQIPLGL-- ---FNVDPG 4 TKWRMNFFRTVTSPKMFPDQDLGGWSS-PDKASFHI------TKFF-----------GHVNF 220\Gibberelia

DDBDRAFT_0183821_Ddis_66802540 -SGIIYGASTI NDGW-EGTLRIPFQL-- ---IQKASQ 6 YIYKFNMFRIDVPLYNYKEYSC-YHADNTNPPCFHV------PSYF-----------GNIAL 231|FG07921.1

TTHERM_01004980_Tthe_146162880 -TGIFYSAQIA DFGY-KANAQIPVKL-- ---VTGTQQ 1 YHLKGNFFRIDYAFNGDIDYSC-WRPTYVSPACFHV------PSQF-----------GDIFL 238|

RcasDRAFT_0067_Rcas_118063192 -ARWWARREDA AGRW-TAILALPWRC-- ---LTSEPL 1 LVWRANLFRIERPRDGEPEFSC-WSPTFTTPADFHK------PSRF-----------GCLML 215|

Acid_6151_Susi_116625206 -YAVKGRIDAP AKVW-YGEMRIPFAA-- ---IDARSP 3 RELRIGLYRI--AGIAPKQYFA-WRPTG--QTSFHV------PQAF-----------GTLRL 220/

SO2192_Sone_24373747 4 -PEVRIQGQWA 2 DVGY-NIELRIPLD--- -----MVG- SKLGFAIADVN---------------------DSKY------RDV------------AAVVG 223\Shewanella SO2192-like

CPS_3981_Cpsy_71279576 3 -PAPQIQGHWL 2 SQGY-NIELRVPLD--- -----NLG- DNIAFAFYDVD----------------------KTN------SEA------------VSAIG 254|

PBPRB0757_Ppro_54302436 2 -AASDIQGFWQ 2 ESGY-NLELRIPLT--- -----MIG- NKLGFAFYDIN---------------------NSRN------RII------------DTIIG 238|

DP0752_Dpsy_51244604 2 -IERRIQGMWR 2 ADGY-AIEIRMPMS--- -----LVG- RKLAFSVGNVN---------------------SSLQ------PRL------------EEIIG 246/

xynA_Tmar_14719787 --RFKTAVKLI EGGY-IVEAAIKWKT-- ---IKPTP- -NTVIGFNIQVND---------------ANEKGQRVGIISWSDPTNNSWRDPSKF--GNLRL 187\CBD family9

xyn10B_Xpac_5880612 --RLTSATART DTGY-VVELAVTLRG-- ---QSGGQD 6 ADTFQGLDVQVND----------------GRDGARYAVHTWADPTGTGYQTGARW--GVAHL 1072|

xynX_Cthe_586269 ----TKYVTSN ADGY-IVQLAIPIED-- ---ISPTL- -NDKIGLDVRLND---------------DKGSGSIDTVTVWNDYTNSQDTNTSYF--GDIVL 705|

xynC_Tsp._4894694 DVEVKKFVGPG FRRY-SFEMSITIPG-- ---VEYRK- -DSYIGFDVAVID---------------------DGKWYSWSDTTNSQKTNTMNY--GTLKL 828|

BBFL7_00085_Fbac_89890737 5 NVVFLSEVSFD ERGW-YVEMKIPYRT-- ---LRFPEQ NVQDWSFQIL------------------------RIIK--SLNEE-YTYNYIDVTT-GTDTQ 227|

MED134_14592_Csp._86132959 6 NVIWESEVTVH DTHW-DIEIRIPWNS-- ---IRFPKK DLSDFTINFQ------------------------RFSG--ALNEE-SFWSPIDPTIDGYLNQ 212|

ATW7_03127_Abac_119468755 8 NAIWDSAGKIT DEGY-VVEMRIPFKA-- ---LRFPED 1 KDLTWGFLLM------------------------RNYP--RDVLYQISNMGFDRDVKCSLCQ 230|

RB2501_14839_Rbif_88805626 6 DAAWTVRTQVG DFGW-SAEFAIPFRS-- ---LRFASG SAKSWGMNFQ------------------------RNIA--KNT-ETAYWTTLPLGFDLKRLS 245|

M23134_01584_Mmar_124008041 6 DGFWKVRTSRT DEGW-IAEMRIPWAT-- ---IRYPKG DHQEWGINFV------------------------RRAR--RVN-EISSWAAYPRAFNPYRMS 234|

ALPR1_00920_Asp._126645907 10 DNKWYAEARIF DTHW-QVEAIIPLST-- ---IRFKDG -AKSWNVNFY------------------------RIDS--HTG-ERSTWSPIPRNLSIISTA 223|

GB2207_03065_Mgam_90417401 5 DGTWYSQTSSN KDYW-YSEIHIPWTV-- ---APMTDA 2 GRKKIALWFS------------------------RVVF--DES-LRFAFPNAYYSRPTFMED 199|

EBAC31A08_Umar_9971878 6 DGDWIAKTQKF DGYW-VSEFFIPWDV-- ---TLMKQP DGDIRKINYT----------------------ALRY----SAEN--ESWVSSAGTMASRNDY 202/

consensus/85% ....................s....h.b.p..............................h.h...........................p......----------------.....

consensus/80% ....................ss.h.h.b.p...s..........................h.h...........................p......----------------.....

Supplementary Fig. 1. Multiple alignment of the DOMON superfamily. Proteins are represented by their gene names, species abbreviations and gis. The coloring reflects the consensus at 80% conservation. The coloring scheme and consensus abbreviations are h, hydrophobic residues (ACFILMVWY), shaded yellow; s, small residues (AGSVCDN), shaded blue; b, big residues (EFHIKLMQRWY), shaded grey; and p, polar residues (CDEHKNQRST) colored red. Ligand interacting residues are shaded red. Species abbreviations are as follows: Abac : Alteromonadales bacterium; Aper : Aeropyrum pernix; Asp. : Algoriphagus sp.; Asp. : Azoarcus sp.; Atha : Arabidopsis thaliana; Bjap : Bradyrhizobium japonicum; CDes : Candidatus Desulfococcus; Cmaq : Caldivirga maquilingensis; Cpsy : Colwellia psychrerythraea; Cthe : Clostridium thermocellum; Ddis : Dictyostelium discoideum; Ddon : Dokdonia donghaensis; Dmel : Drosophila melanogaster; Dpsy : Desulfotalea psychrophila; Dsp. : Dehalococcoides sp.; Fbac : Flavobacteria bacterium; Gpro : gamma proteobacterium; Gzea : Gibberella zeae; Hche : Hahella chejuensis; Hhal : Halorhodospira halophila; Hmar : Haloarcula marismortui; Hsap : Homo sapiens; Idec : Ideonella dechloratans; Mgry : Magnetospirillum gryphiswaldense; Mmag : Magnetospirillum magnetotacticum; Mmar : Microscilla marina; Mmus : Mus musculus; Msed : Metallosphaera sedula; Mthe : Methanosaeta thermophila; Ncra : Neurospora crassa; Pchr : Phanerochaete chrysosporium; Ppro : Photobacterium profundum; Pram : Phytophthora ramorum; Pstu : Pseudomonas stutzeri; Ptun : Pseudoalteromonas tunicata; Pyae : Pyrobaculum aerophilum; Rbif : Robiginitalea biformata; Rcas : Roseiflexus castenholzii; Rsp. : Roseobacter sp.; Rsul : Rhodovulum sulfidophilum; Sagg : Stappia aggregata; Sfum : Syntrophobacter fumaroxidans; Sone : Shewanella oneidensis; Ssol : Sulfolobus solfataricus; Susi : Solibacter usitatus; Tkod : Thermococcus kodakarensis; Tmar : Thermotoga maritima; Tsel : Thauera selenatis; Tsp. : Thermotoga sp.; Tthe : Tetrahymena thermophila; Ugam : uncultured gamma; Umar : uncultured marine; Umay : Ustilago maydis; Xpac : Xylanimicrobium pachnodae.

Note these species abbreviations were used in Fig. 1 and 2