RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowfin Tuna ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Thunnus albacares
FDA Seafood List Common Name: Yellowfin Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970501gel01
Unique Web Code: ynh02Srr
Tissue Aliquot Code: thunalba J96-001 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	24	3.1	0.101178	3	0.542008	9.2	6.7	4.55	24.1	3.2	0.0970676
2	5.2	111	14.2	0.0789209	4	0.58454	10	7.2	5.2	111.5	4.3	0.076982
3	5.85	216	27.6	0.0430345	3	0.203166	3.5	2.5	5.85	215.6	2.7	0.0417685
4	6.55	360	46	0.0982171	6	1.00652	17.1	12.4	6.55	358.6	6.4	0.0890705
5	6.85	392	50.1	0.0171701	4	0.121762	2.1	1.5	6.85	392.2	4.2	0.0164197
6	7.35	464	59.3	0.0401615	3	0.218056	3.7	2.7	7.35	464.1	3.2	0.0387165
7	8.15	588	75.2	0.0986897	6	0.956586	16.3	11.8	8.15	587.6	5.6	0.0962897
8	8.45	652	83.4	0.0670116	6	0.735087	12.5	9	8.45	651.6	6.2	0.0670205
9	8.65	704	90	0.0765881	4	0.511217	8.7	6.3	8.65	703.9	3.9	0.0731333
10	9.3	763	97.6	0.099041	6	0.992229	16.9	12.2	9.3	763.4	6.3	0.0885396

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.393	134	17.2	0.0404103	3	0.212434	0.9	0.6	5.393	134.4	3.1	0.0387781
2	5.601	169	21.8	0.0414561	2	0.178893	0.7	0.5	5.601	168.7	2.5	0.0405866
3	6.2	283	36.4	0.0380437	2	0.150501	0.6	0.4	6.2	282.7	2.1	0.040304
4	6.501	345	44.4	0.0753678	2	0.285144	1.1	0.8	6.501	345.6	2.1	0.077871
5	6.607	361	46.5	0.354266	3	1.75272	7.1	5	6.607	361	2.9	0.343136
6	6.75	376	48.4	0.355204	4	2.17891	8.8	6.3	6.75	376.3	4.3	0.287829
7	6.853	387	49.8	0.377446	2	1.54943	6.2	4.5	6.853	386.6	2.4	0.369935
8	6.956	402	51.7	0.0150383	3	0.0880219	0.4	0.3	6.956	400.8	3.5	0.0143306
9	7.032	413	53.2	0.10926	3	0.537656	2.2	1.5	7.032	413	2.8	0.10848
10	7.093	422	54.3	0.0659872	4	0.455298	1.8	1.3	7.093	421.7	3.9	0.0666459
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.23	442	56.9	0.488609	3	2.33189	9.4	6.7	7.23	441.8	2.7	0.482268
12	7.285	450	57.9	0.10887	2	0.177571	0.7	0.5	7.285	449.9	1.5	0.0664899
13	7.34	458	58.9	0.161948	9	2.4689	9.9	7.1	7.34	460.2	9.4	0.148814
14	7.506	483	62.2	0.181775	2	0.734678	3	2.1	7.506	482.9	2.3	0.179669
15	7.645	504	64.9	0.366829	3	1.84146	7.4	5.3	7.645	503.8	2.8	0.374467
16	8.384	631	81.2	0.0339954	8	0.419923	1.7	1.2	8.384	633.1	7.9	0.0299709
17	8.537	668	86	0.362951	4	2.45201	9.9	7.1	8.537	666.8	4.3	0.320653
18	8.744	706	90.9	0.189373	18	5.66937	22.8	16.3	8.744	701.6	18	0.177703
19	9.339	760	97.8	0.186063	4	1.33862	5.4	3.9	9.339	759.9	4.1	0.182886

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	17	2.2	0.0447618	4	0.304461	5.3	3.7	4.55	17.7	3.9	0.0443616
2	5.2	99	12.7	0.0499657	5	0.464816	8	5.6	5.2	100.2	5.3	0.0492729
3	5.85	213	27.4	0.0322505	4	0.237625	4.1	2.9	5.85	213.6	4.4	0.0305167
4	6.55	357	45.9	0.119898	6	1.12929	19.6	13.7	6.55	356.2	5.7	0.112653
5	6.85	388	49.9	0.0300882	7	0.308426	5.3	3.7	6.85	388.6	7	0.024964
6	7.35	462	59.4	0.0450153	3	0.228909	4	2.8	7.35	462.2	3	0.042985
7	8.15	579	74.4	0.117867	5	1.11591	19.3	13.5	8.15	578.6	5.5	0.114965
8	8.45	646	83	0.079888	7	0.943592	16.3	11.4	8.45	644.7	6.9	0.0772085
9	8.65	698	89.7	0.0807696	4	0.550507	9.5	6.7	8.65	697	3.9	0.0802499
10	9.3	757	97.3	0.0719918	4	0.491003	8.5	6	9.3	756.3	3.8	0.0729447

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	14	1.8	0.0251765	4	0.16773	0.8	0.5	4.55	14.3	4.2	0.0226425
2	5.2	100	12.9	0.038694	4	0.265422	1.2	0.8	5.2	99.3	4.1	0.0365921
3	5.343	124	16	0.0142187	4	0.081708	0.4	0.2	5.343	123.6	3.6	0.0128276
4	5.534	156	20.2	0.0245579	9	0.284917	1.3	0.8	5.534	157	8.7	0.0184652
5	5.85	209	27	0.010075	5	0.0758138	0.4	0.2	5.85	209.7	4.7	0.0091756
6	6.217	283	36.6	0.011593	3	0.0601208	0.3	0.2	6.217	283.5	3	0.0114572
7	6.495	339	43.9	0.0484352	3	0.243759	1.1	0.7	6.495	339.5	2.9	0.0473121
8	6.589	354	45.8	0.29257	2	1.29331	6	3.7	6.589	354.5	2.4	0.301138
9	6.746	370	47.9	0.295526	4	1.7	7.9	4.9	6.746	370.9	3.7	0.259604
10	6.843	380	49.2	0.332046	3	1.48125	6.8	4.3	6.843	380.3	2.6	0.318759
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.955	396	51.2	0.0135243	2	0.0373451	0.2	0.1	6.955	396.2	1.5	0.0135939
12	7.037	408	52.8	0.0814308	3	0.447124	2.1	1.3	7.037	407.4	3.3	0.0769122
13	7.099	417	53.9	0.0531898	3	0.183722	0.8	0.5	7.099	415.3	2.9	0.0359736
14	7.154	425	55	0.0262537	7	0.349102	1.6	1	7.154	420.2	7.2	0.0272622
15	7.236	437	56.5	0.458159	2	1.95863	9.1	5.6	7.236	436.7	2.4	0.462536
16	7.297	446	57.7	0.0435083	1	0.0556543	0.3	0.2	7.297	443.5	1.4	0.0221023
17	7.352	454	58.7	0.132473	5	1.17471	5.4	3.4	7.352	454.9	5.1	0.128729
18	7.505	477	61.7	0.132805	2	0.573094	2.7	1.6	7.505	477.1	2.4	0.13556
19	7.631	496	64.2	0.360113	3	1.90299	8.8	5.5	7.631	495.6	3	0.362114
20	8.15	574	74.3	0.0975039	5	0.723913	3.3	2.1	8.15	572.9	5.2	0.0779323
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.516	657	85	0.280139	7	2.72252	12.6	7.8	8.516	655.4	6.5	0.235695
22	8.691	697	90.2	0.175478	18	5.08619	23.5	14.6	8.691	696.4	18	0.159424
23	9.322	754	97.5	0.11654	4	0.756515	3.5	2.2	9.322	753.5	3.7	0.114586

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	5.385	131	17	0.0470703	5	0.335044	1.3	0.8	5.385	132.5	5.4	0.0349263
2	5.576	161	20.9	0.0433099	3	0.231034	0.9	0.6	5.576	161.1	3	0.0431956
3	6.21	277	36	0.049416	2	0.195465	0.8	0.5	6.21	277.5	2.3	0.0485933
4	6.506	337	43.8	0.0958958	2	0.345706	1.3	0.9	6.506	336.8	2.1	0.0935853
5	6.619	353	45.8	0.323073	3	1.47653	5.7	3.7	6.619	352.8	2.7	0.313466
6	6.708	362	47	0.100195	2	0.342046	1.3	0.9	6.708	362.5	1.8	0.104588
7	6.768	368	47.8	0.321259	3	1.57747	6.1	4	6.768	368.7	3	0.30075
8	6.869	379	49.2	0.397577	3	1.66457	6.4	4.2	6.869	379.3	2.5	0.373757
9	6.973	394	51.2	0.0460698	5	0.377011	1.4	1	6.973	395.2	5.5	0.0387021
10	7.049	405	52.6	0.149427	3	0.803709	3.1	2	7.049	404.9	3.1	0.144572
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	7.112	414	53.8	0.0999385	4	0.705662	2.7	1.8	7.112	413.6	3.9	0.102388
12	7.153	420	54.5	0.0329147	2	0.104958	0.4	0.3	7.153	420.5	2	0.0289001
13	7.237	432	56.1	0.536497	2	2.28499	8.8	5.8	7.237	431.8	2.4	0.526725
14	7.292	440	57.1	0.132188	1	0.153789	0.6	0.4	7.292	439.2	1.5	0.0598311
15	7.341	447	58.1	0.210963	9	3.04802	11.7	7.7	7.341	448	9.3	0.185783
16	7.492	470	61	0.203631	2	0.836356	3.2	2.1	7.492	469.9	2.4	0.198108
17	7.631	491	63.8	0.4218	3	2.18141	8.4	5.5	7.631	490.9	2.9	0.431335
18	8.404	625	81.2	0.0364545	7	0.420091	1.6	1.1	8.404	624.8	6.8	0.034872
19	8.531	658	85.5	0.368183	3	2.122	8.1	5.4	8.531	658.1	3.5	0.344021
20	8.721	698	90.6	0.188146	18	5.49058	21.1	13.9	8.721	697.8	18	0.172099
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.333	753	97.8	0.206062	4	1.36268	5.2	3.4	9.333	752.6	3.8	0.201715

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	8	1	0.0522078	2	0.237598	5	3.6	4.55	8.7	2.5	0.0546373
2	5.2	106	13.8	0.0740294	4	0.501605	10.6	7.6	5.2	105.7	4	0.0712481
3	5.85	201	26.1	0.0390632	3	0.188455	4	2.9	5.85	201.3	2.8	0.0378182
4	6.55	344	44.7	0.0962628	5	0.86087	18.1	13	6.55	343.6	5.3	0.0919971
5	6.85	374	48.6	0.0176646	5	0.144235	3	2.2	6.85	375	4.7	0.0173476
6	7.35	445	57.8	0.0303968	3	0.156878	3.3	2.4	7.35	445.2	2.9	0.0306206
7	8.15	568	73.8	0.0942551	5	0.881311	18.6	13.4	8.15	568.7	5.3	0.0937889
8	8.45	633	82.2	0.0813407	7	0.956092	20.2	14.5	8.45	632.6	6.6	0.0817421
9	8.65	692	89.9	0.0641117	4	0.505617	10.7	7.7	8.65	691.2	4.4	0.0646429
10	9.3	750	97.4	0.0498496	3	0.311935	6.6	4.7	9.3	749.7	3.4	0.0516319

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 93

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.200 5.200 10.000 5.850 3.500 6.550 17.100 6.850 2.100 7.350 3.700 8.150 16.300 8.450 12.500 8.650 8.700 9.300 16.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
5.393 0.900 5.601 0.700 6.200 0.600 6.501 1.100 6.607 7.100 6.750 8.800 6.853 6.200 6.956 0.400 7.032 2.200 7.093 1.800 7.230 9.400 7.285 0.700 7.340 9.900 7.506 3.000 7.645 7.400 8.384 1.700 8.537 9.900 8.744 22.800 9.339 5.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 19 Return to Lane Navigator
4.550 5.300 5.200 8.000 5.850 4.100 6.550 19.600 6.850 5.300 7.350 4.000 8.150 19.300 8.450 16.300 8.650 9.500 9.300 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.550 0.800 5.200 1.200 5.343 0.400 5.534 1.300 5.850 0.400 6.217 0.300 6.495 1.100 6.589 6.000 6.746 7.900 6.843 6.800 6.955 0.200 7.037 2.100 7.099 0.800 7.154 1.600 7.236 9.100 7.297 0.300 7.352 5.400 7.505 2.700 7.631 8.800 8.150 3.300 8.516 12.600 8.691 23.500 9.322 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 23 Return to Lane Navigator
5.385 1.300 5.576 0.900 6.210 0.800 6.506 1.300 6.619 5.700 6.708 1.300 6.768 6.100 6.869 6.400 6.973 1.400 7.049 3.100 7.112 2.700 7.153 0.400 7.237 8.800 7.292 0.600 7.341 11.700 7.492 3.200 7.631 8.400 8.404 1.600 8.531 8.100 8.721 21.100 9.333 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 21 Return to Lane Navigator
4.550 5.000 5.200 10.600 5.850 4.000 6.550 18.100 6.850 3.000 7.350 3.300 8.150 18.600 8.450 20.200 8.650 10.700 9.300 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowfin Tuna ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowfin Tuna ---- Go to Display Gel Lane Navigator.