VUOPIO-VARKILA J, SWENSON J, KILLGORE G, HILL B, MCALLISTER S, TENOVER FC; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 207 (abstract no. 874).
CDC, Atlanta, GA.
MRSA-Screen[TM] (Denka Seiken Co., Ltd, Japan) is a latex agglutination test designed to detect the PBP2' of methicillin-resistant staphylococci. We evaluated this kit with 301 well-characterized staphylococcal isolates: 100 Staphylococcus aureus (51 methicillin-resistant [MRSA] and 49 methicillin-susceptible [MSSA]) and 201 coagulase-negative staphylococci (CNS) (several different species; 128 methicillin-resistant and 73 methicillin-susceptible). The presence of methicillin resistance was confirmed by mecA-PCR and by oxacillin minimum inhibitory concentration (MIC) determination. A positive agglutination reaction occurred with 48 (94%) of 51 MRSA strains, but no (0/49) MSSA strains. The 3 agglutination-negative MRSA strains were positive on retesting. Thirty-seven (73%) MRSA strains showed a positive reaction at 3 min, 5 additional strains at 6 min (cumulatively 83%), and 6 additional strains at 15 min (94%). Among CNS strains, 92 (72%) of 128 methicillin-resistant isolates were positive initially while all methicillin-susceptible strains were negative. When the agglutination-negative methicillin-resistant CNS isolates were retested, 28 additional strains were positive; 8 (6%) remained negative. For CNS isolates, a positive reaction was visible for 50 (40%) at 3 min, 27 (cumulatively 61%) at 6 min, and 43 (95%) at 15 min. In conclusion, the MRSA-Screen[TM] test showed a high predictive value for detection of methicillin resistance in S.aureus (94% initial positives) and CNS (72% initial positives) strains. No false-positive agglutination reactions were seen among the methicillin-susceptible staphylococci tested. The agglutination reaction was, however, often delayed for 6-15 min, suggesting that the final test reading should be at 15 min, not 3 min.
Publication Types:
Keywords:
- Agglutination
- Agglutination Tests
- Evaluation Studies
- Japan
- Latex Fixation Tests
- Methicillin Resistance
- Microbial Sensitivity Tests
- Oxacillin
- Polymerase Chain Reaction
- Staphylococcal Infections
- Staphylococcal Skin Infections
- Staphylococcus
- Staphylococcus aureus
- immunology
- methods
Other ID:
UI: 102245189
From Meeting Abstracts