Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Skipjack Tuna  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Katsuwonus pelamis
FDA Seafood List Common Name: Skipjack Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970501gel06
Unique Web Code: skh02Srr
Tissue Aliquot Code: katspela E96-002 #04a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.553240.062490340.4184215.344.5532.83.90.0605769
25.213116.50.088714860.82940910.67.95.2131.85.60.083123
35.8523529.60.13930940.85083710.88.15.85234.63.50.136509
46.5537647.40.099284291.3891717.713.26.55374.98.80.0892668
56.8541051.70.044826350.3445724.43.36.854104.70.0415573
67.3548360.90.094488540.6341958.167.35483.340.0888315
78.15603760.085741171.0688913.610.28.15603.27.40.0810951
88.4566784.10.12639971.4520118.513.88.45666.670.116349
98.6572391.20.095847850.8076310.37.78.65722.850.0907399
109.378098.40.017332220.06174020.80.69.37802.10.0167518
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.87379100.036666690.48392531.84.87381.390.0304598
25.03310112.70.016311540.1383680.80.55.033102.34.30.0181635
35.35815018.90.015339780.2023221.20.85.358150.97.90.0145297
45.49217221.70.019514960.2311071.40.95.492172.36.30.0206335
56.46436045.40.053342420.237521.50.96.464359.82.50.0546877
66.526373470.03615440.2441391.50.96.5263724.20.0325315
76.56838047.90.046882120.09519790.60.46.568380.51.50.035046
86.62138648.70.083657150.64584742.56.621386.34.50.0801144
96.68239349.60.26382221.083676.64.16.682393.32.20.273307
106.77140350.80.071168230.3523742.21.36.771403.42.80.0712039
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.88641752.60.21329731.187217.34.56.886417.23.30.205414
126.95742753.80.024672960.2527681.516.9574246.30.0228018
137.04343955.40.36375231.8810211.57.27.043439.12.90.365189
147.10744856.50.079109240.5448113.32.17.107447.73.70.0824966
157.22946558.60.068186340.4676762.91.87.229464.94.20.0632091
167.34348160.70.13860351.276667.84.97.3434805.20.139797
177.4750063.10.14018530.7368134.52.87.47499.52.80.146033
187.60352065.60.20855831.064436.54.17.603520.12.80.21612
197.96357472.40.34694731.7110210.56.57.963574.52.70.359976
208.457666840.015175340.1069180.70.48.457665.740.0150773
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.57469988.10.04801890.4630982.81.88.574700.38.90.0292341
228.71372691.60.120231122.3919614.69.18.713727.3120.112462
239.30677898.10.097354230.5446653.32.19.306778.13.20.0967461
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55384.80.036550350.3160193.92.84.5537.55.10.0352395
25.211814.90.040011570.5040936.24.55.2118.97.20.0396111
35.8522828.70.10829140.86195510.67.75.85227.74.50.108565
46.55381480.0886391.216051510.86.55380.58.60.0799405
56.8541552.30.050823860.4701655.84.26.85415.15.70.0461341
67.3548260.70.086474740.5911767.35.37.354824.10.0823418
78.1560275.80.094910481.1880714.610.68.15601.67.60.087973
88.4566283.40.14264781.87212316.78.45661.77.80.135693
98.6571990.60.10685150.86515610.67.78.65718.44.80.100842
109.377697.70.020811880.24081832.19.3773.480.0170421
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55415.20.046221440.3444132.21.74.55414.40.044211
24.868210.30.015708580.2114321.414.8685.87.70.0154504
35.2127160.03853150.30408621.55.2126.94.60.0374327
45.492175220.009974150.08106850.50.45.492174.84.50.0101039
55.8523429.40.060824840.4243912.82.15.85233.43.90.0608942
66.46736345.70.027338320.1058570.70.56.467363.520.029296
76.53437747.40.037332840.2631151.71.36.534375.94.10.0359288
86.58238448.30.034541720.08342040.50.46.582384.31.70.0275075
96.64439149.20.052619540.3430742.21.76.644390.43.50.0552543
106.68839649.80.33586821.11737.35.56.688396.11.80.341028
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.77640651.10.047035450.3871282.51.96.776407.85.20.0422843
126.87741852.60.19778831.0761175.36.877418.73.20.189101
137.03844055.30.33305731.53156107.67.038440.42.50.339799
147.09644856.40.071854450.61792343.17.096448.84.90.0714162
157.23546758.70.04566350.3635622.41.87.235465.14.90.0418025
167.34548260.60.095136960.9484036.24.77.345481.95.60.0949174
177.48350263.10.103730.5236573.42.67.4835022.90.102589
187.61452165.50.19627831.049786.95.27.614520.83.10.188516
197.90256370.8010007.90256210
207.99957772.60.30837731.7136511.28.57.999576.330.317421
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.1559975.30.0297048120.5775673.82.98.15594.112.50.0260738
228.4566683.80.030368370.3532912.31.88.45666.37.10.0282714
238.55469587.40.0394471161.109257.25.58.554699.915.80.0396612
248.6572290.80.0835187101.280338.46.38.65722.610.50.0688447
259.28477597.50.082562930.498473.32.59.284775.43.40.0831026
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.9158710.90.0184622120.4151722.21.54.91587.711.80.0198889
25.05210613.30.012716240.03464570.20.15.052107.24.20.0046782
35.50117622.10.019898150.1609310.90.65.501175.75.40.0166842
46.45636045.20.042607320.18080910.76.456359.92.40.0428973
56.523374470.027231140.18674810.76.523372.73.90.026995
66.57938348.10.055107920.1811910.76.579382.81.90.0529113
76.62438848.70.080961830.5286262.926.624388.93.40.086593
86.69439649.70.38301621.251926.84.66.694395.91.80.385724
96.782406510.073743320.2778571.516.782406.32.20.0708781
106.90342152.90.24523620.9548535.23.56.903420.32.40.2224
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.98243254.30.013861770.5223972.81.96.982421.76.80.0435723
127.06144355.70.37296831.671899.16.27.061442.82.60.368176
137.11845156.70.090359250.7263393.92.77.118451.54.50.090722
147.2446858.80.064900540.3925322.11.57.24468.33.60.0618633
157.35548460.80.13075551.249916.84.67.355483.35.50.128268
167.48550363.20.14274930.7290513.92.77.485502.52.80.144776
177.61552265.60.26181431.542728.45.77.615522.23.50.249565
187.97957572.20.37483831.8746410.177.979575.22.70.391671
198.45766683.70.018634540.1312070.70.58.457665.640.0183602
208.5769887.70.0865688182.5068613.69.38.57699.6180.078576
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.72572791.30.100166132.0420511.17.68.72572713.40.085964
229.31577897.70.11820540.907074.93.49.315777.84.40.11506
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55334.10.065486850.51814964.34.5534.14.70.0623555
25.212715.90.076497560.8046329.36.75.2128.26.10.0743415
35.8523429.30.16080541.0511712.18.85.85233.53.80.157235
46.5538047.60.10230181.2067613.910.16.55379.38.10.0843562
56.8541451.90.052116750.4300854.93.66.85414.85.10.0479683
67.3548560.80.10430440.799749.26.77.35484.74.40.102101
78.1560275.40.083351181.10312.79.28.15601.98.40.0743205
88.4566383.10.12563181.7207719.814.48.45662.580.120913
98.6572090.20.10835250.88119110.17.48.657204.80.103816
109.377897.50.0073191140.18262.11.59.3770.413.80.0074725
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
blue spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
red spacer for gel schematic
white spacer for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
white spacer for gel schematic

 
Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 100
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse   |   RFE Tutorial   |   Seafoods   |   CFSAN


Last updated on 2003-FEB-27 by bjt/frf/nln