Fred J. Stevens

Bldg: 202 Room: B229
Email: fstevens@anl.gov

Phone: (630) 252- 3837

 

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Education
Professional Experience
Publication List
Research Highlights

Full Information

> Education:

• 1976 Ph.D Biophysics Northwestern University
• 1974 M.S. Physics Northwestern University
• 1971 B.A. Physics Hamline University

> Professional Experience:

• 1/01-present: Senior Biophysicist; Biosciences Divsion
  Argonne National Laboratory
• 8/87-12/00: Biophysicist; Biosciences Division,
  Argonne National Laboratory.
• 1/84 7/87: Assistant Biophysicist; Division of Biological and
  Medical Research (BIM), Argonne National Laboratory.
• 8/81 12/83: Project Manager, Research Biochemist; Diagnostics
  Division, Abbott Laboratories.
• 2/81 7/81: Assistant Scientist, BIM, Argonne National Laboratory
  (Term Appointment).
• 11/77 1/81: Postdoctoral Appointee, BIM,
  Argonne National Laboratory.
• 1/76 10/77: Research Associate, Department of Microbiology and
  Public Health, Michigan State University.
• TEACHING EXPERIENCE: Argonne National Laboratory maintains several formal programs that support education and training of students at the undergraduate, graduate, and post-graduate levels. Undergraduate students participate in research projects in my laboratory on an annual basis. Training includes formal and informal lectures that cover fundamentals of the methods that are used, as well as presentation of the larger context and significance of the studies in which they participate. A graduate student recently completed her PhD (IIT) undermy supervision. I have participated on the graduate committee of two graduate students who have obtained their PhD. I have supervised or participated in the supervision and development of 10 postdoctoral students. One of these has been recently hired onto the scientific staff of Argonne National Laboratory.
• CONSULTANTSHIPS AND ADJUNCT APPOINTMENTS:
  4/93--present: Affiliate, University of Chicago Cancer Institute
  2/90 present: University of Tennessee Medical Center at Knoxville. Visiting Scientist
  4/85 4/88: Abbott Laboratories. Consultant, Chemical and Agricultural Products Division.
  1/82 12/83: Consultant, BIM, Argonne National Laboratory.
  12/78 12/80: Guest Research Associate. Division of Biology, Brookhaven National Laboratory.

> Research Highlights:

Analyses of protein primary structures to identify correlates of pathological properties with emphasis on the immunoglobulin superfamily and related beta-domain proteins. Protein:protein interactions; protein structure and function; physical principles in biological systems; computer modeling for enzyme modification and computer simulation of macromolecular interactions. Development of chromatography systems and computer simulations for the analysis of the kinetics and affinity of macromolecular interaction including antibody:antigen, anti idiotope:idiotope, rheumatoid factor:IgG, antibody:peptide and protein:nucleic acid interactions. Development of chromatographic methods for the identification and characterization of pathological immunoglobulin light chains formed during multiple myeloma and related diseases. Recognition of protein structural and functional similarities at minimal levels of amino acid sequence identity.

> Patents

1. Stevens, F.J. 1988. Size exclusion chromatography system for macromolecular interaction analysis. No. 4,762,617
2. Stevens, F.J. 1991. A method of field flow fractionation wherein the polarity of the electric field is periodically reversed No. 5,133,844
3. Stevens, F.J. and S. P. Tsai. Preparation of acrylic acid from lactic acid via modified fumarase. Abandoned.
4. Stevens, F.J., A. Solomon, and E. Myatt. 2000. Method for detecting and diagnosing disease caused by pathological protein aggregation. No. 6,063,636
5. Stevens, F.J. Method for producing fabrication material for constructing micrometer scaled machines, fabrication material for micrometer scaled machines. Pending.
6. Stevens, F.J., R. Raffen, P. Wilkins Stevens, and M. Schiffer. 2002. Method for altering antibody light chain interactions. No. 6,485,943
7. Stevens, F.J. and P. Wilkins-Stevens. Device for detecting actinides, method for detecting actinides. Pending
8. Stevens, F.J., M. Schiffer, P. Wilkins Stevens, W. Carey Hanly, and S.L. Tollaksen. Device for detecting molecules, method for detecting molecules. Pending.
9. Stevens, F.J., Y. Argon, D. Davis, and R. Raffen. A fibril-blocking peptide, a method for preventing fibril formation. No. 6,878,521
10. Stevens, F.J. 2003. System and method for a parallel immunoassay system. 2003 No. 6,489,120
    
    
    INVENTION REPORTS:

1. Stevens, F.J. 1986. Size exclusion chromatography system for macromolecular
   interaction analysis. ANL IN 86 018, DOE: S 64,667.
2. Stevens, F.J. 1987. One step viral immunoassay. ANL IN 87 107, DOE: S 67,394.
3. Stevens, F.J. and D.A. LeBuis. 1988. Pulsed field field flow fractionation (PF4).
   ANL IN 88 072, DOE: S68,647.
4. Stevens, F.J. 1990. Nephromimitic chromatography for identification and diagnosis
   of pathological proteins. ANL IN 90 011, DOE: S 71,196.
5. Stevens, F.J. 1990. Antibody characterization workstation. ANL IN 017,
   DOE: S 71,319.
6. Stevens, F.J. 1990. Nonlinear electroimmuno detector device. ANL IN 038,
   DOE: S 71,992.
7. Stevens, F.J. 1991. Micrometer design and construction by self assembly of
   nanometer scale engineered proteins. ANL IN 91 101, DOE: S 75,872.
8. Stevens, F.J. 1992. Automated analytical assay involving magnetic separation.
   ANL IN 92 045, DOE: S 76,933.
9. Stevens, F.J. and S. P. Tsai. 1992. A process for conversion of lactic acid into acrylic acid
   catalyzed by a modified fumarase. ANL IN 92 060.
10. Stevens, F.J. and M. Schiffer. 1993. Protein engineering strategies for optimized
    production of recombinant antibodies. ANL IN 93 108
11. Stevens, F.J. 1994. Engineering of proteins to create detectors for actinides and
    other heavy metals. ANL IN 94 144, DOE: S-83,527.
12. Stevens, F.J. 1994. Drug design strategies to inhibit amyloid fibril formation.
    ANL IN 94 145, DOE S-83,528.
13. Stevens, F.J. 1995 Method to evoke a cellular immune response to in situ amyloid deposits.
    ANL-IN-95-019, DOE S84,206
14. Stevens, F.J. 1995. A novel biomaterial substance derived from a biological fibril.
    ANL-IN-95-040, DOE S-84,207
15. Stevens, F.J. 1995. PROSPECDS (Protein SequencePoly-Evaluation, Comparison, &
    Display Systems) ANL-SF-95-068
16. Stevens, FJ. 1996. 30 Second Immunoassay. ANL-IN-96-062, DOE S-86,391
17. Stevens, F.J. 1996. Use of genetically engineered plants to produce human biomolecules.
    ANL-IN-96-075, DOE S-86,844
18. Stevens, F.J. 1996. Immunassay for detection of biological agents. . ANL-IN-96-122,
    DOE-S87,875
19. Stevens, F.J. 1997. Massively Parallel Immunoassay (MPIA). ANL-IN-97-055, DOE S89,003
20. Stevens, F.J., M. Schiffer, K. Nash, and M. Jensen. 1997. Designs for actinide binding sites at
    the interface of a protein dimer. ANL-IN-97-056, DOE S89,002
21. Stevens, F.J. and M. Schiffer. 1998. Janusbody: a compact molecule with antibody-antigen
    binding characteristics. ANL-IN-98-021, DOE S90,947.
22. Stevens, F.J., and R. Raffen. 1998. In vitro protein fibril production: potential high-throughput
    method to screen for anti-amyloid drug leads. ANL-IN-98-046, DOE S91,201
23. Stevens, F.J., C.S. Giometti, and A. Joachimiak. 1998. The anti-western blot.
24. Stevens, F.J. and P. Wilkins Stevens. 1998. Immunological assays for actinides. ANL-IN-98-086,
    DOE S91,835
25. Stevens, F.J., Y. Argon, D. Davis, and R. Raffen. 1999. Composition of a fibril-blocking peptide; strategy for development of peptides to inhibit conformational disease resulting from protein domain swapping. ANL-IN-99-019, DOE S93,203
26. Argon, Y., D.P. Davis, F.J. Stevens, and R. Raffen. 2000. A method for inhibiting amyloid-like protein aggregation with peptides. UCHI # 820.
27. Stevens, F.J. 2000. A method for high-throughput screening of protein nucleic acid (PNA) libraries for ligand (protein-binding) activity. ANL-IN-00-13
28. Stevens, F.J. and F. Collart. 2000. Universal Genomic Detector: A generic method for identification and comparison of genomic material. ANL-IN-00-014
29. Stevens, F.J. 2000.Cyber cell: Molecular beacon methodology for a live-cell/microprocessor interface. ANL-IN-00-15
30. Stevens, F.J. and J.F. Carpenter. 2000. Lethal ligands: From conformational disease to
    conformational therapy. ANL-IN-00-22
31. Stevens, F.J. 2000. Biosentinel: Device for autonomous monitoring and evaluation of
    environmental samples. ANL-IN-00-52
32. Stevens, F.J. and M. Schiffer. 2000. Process for customizing functional stability of antibodies.
33. Stevens, F.J. 2000. Protein nucleic acids: methodology for generation and detection of forensic signatures.
34. Stevens, F.J. 2001. Differential PCR PNA-clamped SNP diagnostics. ANL-IN-01-101.
35. Stevens, F.J. 2001. Peptidyl chaperones. ANL-IN-01-101.
36. Stevens, F.J. 2003. Method for producing antibodies to proteins from select against without access to select agents; method for producing antibodies to proteins that cannot be produced for experimental reasons. ANL-IN-03-015
37. Vilim, R., and F.J. Stevens. 2003. Parsimonious fold-specific protein classifier from attribute-constrained genetic algorithms. ANL-IN-03-003.
38. Stevens, F.J. 2003. A thermophilic organophosphate detoxification enzyme. ANL-IN-03-051
39. Stevens, F.J., L. Chen, B. Kay. 2003. Functionalized three-dimensional protein lattices formed by self assembly. ANL-IN-03-52
40. Stevens, F.J. 2003. A systematic strategy for the disruption of adhesion protein-based biofilms of medical and commercial significance. ANL-IN-03-115

> Honors/Awards

• Editorial Board: Amyloid:The International Journal of Experimental and Clinical Investigation
  (1999-present)
• NIH Postdoctoral Fellowship. 1978. Awarded for project initiated at
  Michigan State University. Declined.
• Fellowships from Physics Dept., Northwestern University (1971 1973)
• National Merit Scholarship (1966 1971)
  
  MEMBERSHIPS IN PROFESSIONAL SOCIETIES:
  
  Biophysical Society (active)
  Protein Society (active)
  American Association of Immunologists (active)
  American Association for the Advancement of Science (active)
  International Society of Amyloidosis (active)
  International Interest Group in Biorecognition Technology
  American Society for Cell Biology
  American Society for Microbiology

> Grant Support

Source: NIH
Project: "Biophysics of Myeloma Pathology" (05/01/92 03/31/06)

Source: DOE (ER/LTT)
Project: "Exploratory Research to Identify
Anti Amyloid Drugs" (CRADA) (9/01/93 12/30/96)

Source: DOE (NN-20)
Project : "Ultrasensitive Actinide Detection Through
Bio-based Receptors" (12/1/96 -09/30/00)

Source: NIH
Project: “Protein Structure, Stability, and Aging” (04/15/00 – 04/14/04)