| US 7,361,517 B2 | ||
| Real time method of detecting acute inflammatory conditions | ||
| Joseph M. Ahearn, Sewickley, Pa. (US); and Susan M. Manzi, Wexford, Pa. (US) | ||
| Assigned to University of Pittsburgh, Pittsburgh, Pa. (US) | ||
| Filed on Apr. 08, 2005, as Appl. No. 11/101,898. | ||
| Claims priority of provisional application 60/579621, filed on Jun. 14, 2004. | ||
| Claims priority of provisional application 60/560986, filed on Apr. 09, 2004. | ||
| Prior Publication US 2005/0282234 A1, Dec. 22, 2005 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 436—811 [435/7.1; 435/7.92; 436/501; 436/518] | 12 Claims |
| 1. A method for diagnosing an acute inflammatory episode of systemic lupus erythematosus (SLE) in an individual, the method
comprising:
(a) isolating reticulocytes from a blood sample of an individual;
(b) determining the level of a C4d complement pathway component on the isolated reticulocytes from the individual, and
(c) comparing the C4d complement pathway component level on the isolated reticulocytes from the individual with a control
level of C4d complement pathway component on reticulocytes, wherein an elevated level of the C4d complement pathway component
on the isolated reticulocytes in the individual compared to the control level of the C4d complement pathway component on reticulocytes
indicates that the individual has the acute inflammatory episode of SLE.
|