Blazquez J, Espinosa de los Monteros LE, Samper S, Martin C, Van Embden J, Guerrero A, Cobo J, Gomez-Mampaso E, Baquero F; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1996 Sep 15-18; 234 (abstract no. J87).
Hospital Ramon y Cajal, Spain.
Nineteen multidrug-resistant (MDR) Mycobacterium complex isolated in a hospital nosocomial outbreak were characterized at molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting they were M. bovis. All of them were resistant to isoniazid, rifampin, pyrazinamide, ethambutol, streptomycin, para-aminosalicylic acid, clarithromycin, cycloserin, ethionamide, ofloxacin, capreomycin and amikacin. By performing the standardized IS6110 fingerprinting by restriction fragment length polymorphism (RFLP) we were able to differentiate two groups, A and B, containing two (16 isolates) and three (3 isolates) IS6110 copies respectively. These strains were typed by spoligotyping, developed to distinguish M. bovis strains and also to distinguish them from M. tuberculosis (Van Embden et al, submitted). All the strains were confirmed to be M. bovis. In addition, spoligotyping showed only the difference in one spacer of 43 between the groups RFLP-A and RFLP-B. These patterns were different to those of two hundred M. bovis strains of the spoligotype-RIVM data base, suggesting a specific spoligotype of these MDR-M. bovis. The rpobeta region of several strains representative of each identified group was cloned and sequenced showing identical mutation (Ser-531 to Leu) responsible for the rifampicin resistance phenotype. To our knowledge this is the first characterization at molecular level of a M. bovis responsible for the hospital outbreak. The data here presented strongly suggest that the strains characterized are members of a MDR-M. bovis clone family.
Publication Types:
Keywords:
- Base Sequence
- Disease Outbreaks
- HIV Seropositivity
- Humans
- Mycobacterium bovis
- Polymorphism, Restriction Fragment Length
- Rifampin
- Tuberculosis
- genetics
Other ID:
UI: 102235138
From Meeting Abstracts