Roques P, Parnet-Mathieu F, Marce D, Herve F, Dolfus F, Meyohas MC, Winter C, Courpotin C, Dormont D; International Conference on AIDS.
Int Conf AIDS. 1993 Jun 6-11; 9: 309 (abstract no. PO-B05-1040).
Hopitaux de Paris, France.
OBJECTIVE. To investigate the biological parameters of HIV infection of newborns during the perinatal period of time. METHODS. Patient Population included 100 infant mother-couples between october 89 and 92. PCR: 3 couples of primers (specific for gag, pol and env gene) were used in the same reaction mixture. Control of contamination and sensitivity of 10 HIV copies per million of cells was used. Virus isolation: PBMC were isolated by ficoll gradient and cocultivated with normal human cord lymphocytes (2/1). HIV production was tested using p24 antigen detection (Abbott EIA) and reverse transcriptase assay. An infectious status was given for 2 positive cultures (C+) and 2 positive PCRs (PCR+) Western blot: Pasteur Newlav Blot. RESULTS. 17 newborns (NB) were positive. All these 17 NB are symptomatic at 6 months of life (8 are more than 18 months old). Among them, 12 NB were cultured within the first week of life: 9 were C+ and PCR+ at this time. One was only PCR+. All these C+ were confirmed one month later. 11 were treated with AZT from the 4th month on. 70 NB are not contaminated (2 PCR and 2 cultures negative (C-) within the first six months of life. They never exhibit any specific HIV related clinical or biological symptom. Among these NB, 37 are more than 18 months old and they did not exhibit any HIV serologic specific pattern. 4 of the 13 remaining infants are unevaluable (2 not sampled after 12 months old and 2 age < 12 months). The 9 other NB are more than 18 months old. These NB present 2 or 3 PCR undoubtedly positive (3 primers) between the 1 and the 6th month of life despite the fact that they are negative for PCR during the second year and remain without any clinical symptoms, and are still seronegative. CONCLUSION. Using culture and PCR, 17% NB were diagnosed as infected, 70% are not infected. 9% NB are transiently PCR positive, suggesting either a transient infection or a very week viral load in reservoir cells which is not able to induce a humoral immune response.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Blotting, Western
- Disease Transmission, Vertical
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Infant
- Infant, Newborn
- Polymerase Chain Reaction
- Prospective Studies
- transmission
Other ID:
UI: 102203929
From Meeting Abstracts