Abrescia N, Martinelli P, Sansone M, Busto A, D'Abbraccio M, Figoni M, Viglietti R, Di Nicuolo G, De Marco M, Butrico E, Maddaloni A, Piccirillo M; IAS Conference on HIV Pathogenesis and Treatment (2nd : 2003 : Paris, France).
Antivir Ther. 2003; 8 (Suppl.1): abstract no. 355.
Infectious Diseases Division, "D. Cotugno" Hospital
Few comparative studies to evaluate the various cervicovaginal secretions (CVS) sampling methods for HIV-1 quantitative determinations have been performed. In a perspective study we compared a very used method, the cervicovaginal lavage (CVL) with the direct aspirate of cervical mucous (DACM). We studied 24 HIV-1-positive women with detectable plasma viral load (VL) (13 naives and 11 HAART-experienced). All of them had intact female genital tract (FGT) at gynaecological and ultrasound examinations and a negative PAP test in the previous 12 months. 17/24 women (70%) had CDC C-3 infection. CD4 cells baseline count was <200/ml in 20/24 women (83.3%). HIV-1 RNA was quantitatively determined [PCR ultrasensitive Amplicor Roche test (20 copies/ml)] in simultaneous samples of plasma, DACM and CVL. 7/24 women (4 naives and 3 experienced, all C-3 and with <100 CD4+) after 3 months of HAART underwent a follow-up (FU). Baseline mean plasma VL (5.1 log10) was higher (P<0.01) than both DACM (4.6 log10) and CVL (4.2 log10) VLs. No significant difference between HIV-1 RNA DACM and CVL mean levels was demonstrated. Of FU patients, 3/7 had undetectable plasma VL, 6/7 had undetectable VL in CVL and DACM, 2/7 had undetectable VL in plasma, DACM and CVL. In one case HIV-1 RNA was detectable in DACM and in CVL (mean 3448 copies/ml), but not in plasma. The study indicate that also the less used DACM method can be validated for FGT secretions sampling, that the FGT is a reservoir of HIV-1 and that HAART is efficacious in CVS.
Publication Types:
Keywords:
- Antiretroviral Therapy, Highly Active
- CD4 Lymphocyte Count
- Female
- Genitalia, Female
- HIV-1
- Humans
- Irrigation
- RNA
- RNA, Viral
- Vaginal Smears
- Viral Load
- methods
Other ID:
UI: 102262640
From Meeting Abstracts