Li JJ, Kien AE, Zhang WG, Nicolaides A, Huang YQ; National Conference on Human Retroviruses and Related Infections.

Program Abstr First Natl Conf Hum Retrovir Relat Infect Natl Conf Hum Retrovir Relat Infect 1st 1993 Wash DC. 1993 Dec 12-16; 145.

New York University Medical Center, New York, NY.

FGF-4 (kFGF/hst) was isolated from the DNA to fresh Kaposi's Sarcoma (KS) biopsies by NIH3T3 transfection assay. Although the expression of FGF-4 is not detected in KS derived culture cells, the expression of this oncogene in KS tumor tissue has not been previously examined. The gene amplification and expression of FGF-4 in KS biopsies was examined by differential PCR, Southern blot, and RT-PCR methods. Gene amplification of FGF-4 was found in 4 of 32 (12.5%) specimens of KS, among these 4 samples, three which showed expression of this gene. A total of 6 of 14 (42%) of KS tumor samples showed low FGF-4. However, neither gene amplification nor expression of FGF-4 was detected in normal skin of patients with KS. Like other FGFs, the detection of FGF-4 in KS skin tumors but not in normal skin from the same host suggests that FGF-4 may be involved in the pathogenesis of KS.

Publication Types:

• Meeting Abstracts

Keywords:

• Fibroblast Growth Factors
• Gene Amplification
• Gene Expression
• Humans
• Oncogenes
• Polymerase Chain Reaction
• Proto-Oncogene Proteins
• Sarcoma, Kaposi
• Skin
• Skin Neoplasms
• genetics

Other ID:

• 95921520

UI: 102214460

From Meeting Abstracts