BLASTP 2.2.17 [Aug-26-2007]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schäffer, Alejandro A., L. Aravind, Thomas L. Madden,
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SMT1099
(425 letters)
Database: nr
5,470,121 sequences; 1,894,087,724 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gi|148986006|ref|ZP_01819059.1| hypothetical protein CGSSp3... 740 0.0
gi|15903920|ref|NP_359470.1| hypothetical protein spr1879 [... 740 0.0
gi|148998833|ref|ZP_01826270.1| hypothetical protein CGSSp1... 738 0.0
gi|148989765|ref|ZP_01821074.1| hypothetical protein CGSSp6... 737 0.0
gi|15901886|ref|NP_346490.1| hypothetical protein SP_2067 [... 737 0.0
gi|149004019|ref|ZP_01828826.1| hypothetical protein CGSSp1... 736 0.0
gi|148992092|ref|ZP_01821866.1| glutamyl-tRNA synthetase [S... 723 0.0
gi|111658751|ref|ZP_01409388.1| hypothetical protein SpneT_... 697 0.0
gi|157075124|gb|ABV09807.1| conserved hypothetical protein ... 490 e-137
gi|24380341|ref|NP_722296.1| hypothetical protein SMU.1999c... 474 e-132
gi|84489637|ref|YP_447869.1| predicted glutamylcysteine syn... 231 8e-59
gi|55821872|ref|YP_140314.1| glutamate--cysteine ligase, pu... 202 5e-50
gi|114567570|ref|YP_754724.1| Gamma-glutamylcysteine synthe... 119 5e-25
gi|55821873|ref|YP_140315.1| glutamate--cysteine ligase, pu... 79 4e-13
gi|58040405|ref|YP_192369.1| Glutamate--cysteine ligase [Gl... 68 1e-09
gi|149913419|ref|ZP_01901952.1| glutamate--cysteine ligase ... 66 4e-09
gi|3913791|sp|O22493|GSH1_LYCES Glutamate--cysteine ligase,... 65 1e-08
gi|99078054|ref|YP_611312.1| glutamate--cysteine ligase [Si... 64 2e-08
gi|150395612|ref|YP_001326079.1| glutamate--cysteine ligase... 64 2e-08
gi|3913792|sp|O23736|GSH1_BRAJU Glutamate--cysteine ligase,... 63 3e-08
gi|110590457|pdb|2GWD|A Chain A, Crystal Structure Of Plant... 62 5e-08
gi|16226411|gb|AAL16161.1|AF428393_1 AT4g23100/F7H19_290 [A... 62 5e-08
gi|15912189|gb|AAL08228.1| AT4g23100/F7H19_290 [Arabidopsis... 62 7e-08
gi|488615|emb|CAA82626.1| gamma-glutamylcysteine synthetase... 62 9e-08
gi|1742963|emb|CAA71075.1| gamma-glutamylcysteine synthetas... 61 1e-07
gi|15236390|ref|NP_194041.1| RML1 (PHYTOALEXIN DEFICIENT 2,... 61 2e-07
gi|116295202|gb|ABJ98542.1| gamma-glutamylcysteine syntheta... 61 2e-07
gi|34484367|gb|AAO45821.1| gamma-glutamylcysteine synthetas... 60 2e-07
gi|15964523|ref|NP_384876.1| PUTATIVE GLUTAMATE--CYSTEINE L... 60 2e-07
gi|100801746|emb|CAK24967.1| gamma-glutamylcysteine synthet... 60 2e-07
gi|111380512|gb|ABD98695.2| chloroplast gamma-glutamylcyste... 60 2e-07
gi|126727777|ref|ZP_01743607.1| glutamate--cysteine ligase ... 60 3e-07
gi|31043672|emb|CAD91714.1| glutamate-cysteine ligase [Bras... 60 3e-07
gi|120972552|gb|ABM46854.1| gamma-glutamylcysteine syntheta... 59 5e-07
gi|118735071|ref|ZP_01583544.1| glutamate--cysteine ligase ... 59 6e-07
gi|55823790|ref|YP_142231.1| glutamate--cysteine ligase, pu... 59 6e-07
gi|49475196|ref|YP_033237.1| Glutamate-cysteine ligase [Bar... 59 7e-07
gi|18150411|gb|AAL61610.1|AF401621_1 gamma-glutamylcysteine... 59 8e-07
gi|50058088|dbj|BAD27390.1| gamma-glutamylcysteine syntheta... 58 1e-06
gi|114705940|ref|ZP_01438843.1| glutamate-cysteine ligase p... 58 1e-06
gi|31043670|emb|CAD91713.1| glutamate-cysteine ligase [Bras... 58 2e-06
gi|6651031|gb|AAF22137.1|AF128455_1 gamma-glutamylcysteine ... 57 2e-06
gi|121602200|ref|YP_988623.1| glutamate--cysteine ligase [B... 57 2e-06
gi|3688156|emb|CAA06613.1| gamma-glutamylcysteine synthetas... 57 3e-06
gi|55821875|ref|YP_140317.1| glutamate--cysteine ligase, pu... 57 3e-06
gi|86356449|ref|YP_468341.1| glutamate-cysteine ligase prot... 56 4e-06
gi|49473946|ref|YP_031988.1| Glutamate-cysteine ligase [Bar... 56 5e-06
gi|57903694|gb|AAW58147.1| gamma-glutamylcysteine synthetas... 56 5e-06
gi|11386873|sp|Q9ZNX6|GSH1_MEDTR Glutamate--cysteine ligase... 55 7e-06
gi|17154688|emb|CAC83005.1| putative gamma-glutamylcysteine... 55 9e-06
>gi|148986006|ref|ZP_01819059.1| hypothetical protein CGSSp3BS71_05174 [Streptococcus pneumoniae
SP3-BS71]
gi|147921979|gb|EDK73104.1| hypothetical protein CGSSp3BS71_05174 [Streptococcus pneumoniae
SP3-BS71]
Length = 425
Score = 740 bits (1910), Expect = 0.0, Method: Composition-based stats.
Identities = 371/425 (87%), Positives = 392/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT EIQA+ALNGDE++IY
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQAFALNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YKFLRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKFLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|15903920|ref|NP_359470.1| hypothetical protein spr1879 [Streptococcus pneumoniae R6]
gi|116515452|ref|YP_817283.1| hypothetical protein SPD_1878 [Streptococcus pneumoniae D39]
gi|149023640|ref|ZP_01836143.1| hypothetical protein CGSSp23BS72_04400 [Streptococcus pneumoniae
SP23-BS72]
gi|15459570|gb|AAL00681.1| Hypothetical protein [Streptococcus pneumoniae R6]
gi|116076028|gb|ABJ53748.1| conserved hypothetical protein [Streptococcus pneumoniae D39]
gi|147929739|gb|EDK80730.1| hypothetical protein CGSSp23BS72_04400 [Streptococcus pneumoniae
SP23-BS72]
Length = 425
Score = 740 bits (1910), Expect = 0.0, Method: Composition-based stats.
Identities = 371/425 (87%), Positives = 392/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT EIQA+ALNGDE++IY
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQAFALNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YKFLRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKFLRRQFSKKNLTDEEETMIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|148998833|ref|ZP_01826270.1| hypothetical protein CGSSp11BS70_05040 [Streptococcus pneumoniae
SP11-BS70]
gi|147755394|gb|EDK62444.1| hypothetical protein CGSSp11BS70_05040 [Streptococcus pneumoniae
SP11-BS70]
Length = 425
Score = 738 bits (1904), Expect = 0.0, Method: Composition-based stats.
Identities = 370/425 (87%), Positives = 392/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT+EIQA+ALNGDE++IY
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATSEIQAFALNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YK LRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|148989765|ref|ZP_01821074.1| hypothetical protein CGSSp6BS73_01022 [Streptococcus pneumoniae
SP6-BS73]
gi|149011923|ref|ZP_01833071.1| hypothetical protein CGSSp19BS75_02663 [Streptococcus pneumoniae
SP19-BS75]
gi|147763878|gb|EDK70811.1| hypothetical protein CGSSp19BS75_02663 [Streptococcus pneumoniae
SP19-BS75]
gi|147924881|gb|EDK75964.1| hypothetical protein CGSSp6BS73_01022 [Streptococcus pneumoniae
SP6-BS73]
Length = 425
Score = 737 bits (1902), Expect = 0.0, Method: Composition-based stats.
Identities = 370/425 (87%), Positives = 391/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT EIQA+ALNGDE++IY
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQAFALNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YK LRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|15901886|ref|NP_346490.1| hypothetical protein SP_2067 [Streptococcus pneumoniae TIGR4]
gi|149007782|ref|ZP_01831378.1| hypothetical protein CGSSp18BS74_04391 [Streptococcus pneumoniae
SP18-BS74]
gi|14973578|gb|AAK76130.1| hypothetical protein SP_2067 [Streptococcus pneumoniae TIGR4]
gi|147760632|gb|EDK67605.1| hypothetical protein CGSSp18BS74_04391 [Streptococcus pneumoniae
SP18-BS74]
Length = 425
Score = 737 bits (1902), Expect = 0.0, Method: Composition-based stats.
Identities = 369/425 (86%), Positives = 392/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEV+K LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVMKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT+EIQA+ALNGDE++IY
Sbjct: 241 VNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATSEIQAFALNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YK LRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|149004019|ref|ZP_01828826.1| hypothetical protein CGSSp14BS69_06777 [Streptococcus pneumoniae
SP14-BS69]
gi|147758001|gb|EDK65008.1| hypothetical protein CGSSp14BS69_06777 [Streptococcus pneumoniae
SP14-BS69]
Length = 425
Score = 736 bits (1899), Expect = 0.0, Method: Composition-based stats.
Identities = 369/425 (86%), Positives = 391/425 (92%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT EIQA++LNGDE++IY
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQAFSLNGDEVIIY 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YK LRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|148992092|ref|ZP_01821866.1| glutamyl-tRNA synthetase [Streptococcus pneumoniae SP9-BS68]
gi|147929141|gb|EDK80152.1| glutamyl-tRNA synthetase [Streptococcus pneumoniae SP9-BS68]
Length = 425
Score = 723 bits (1867), Expect = 0.0, Method: Composition-based stats.
Identities = 364/425 (85%), Positives = 387/425 (91%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
MS SIDLLKHRYLKNIKENPELFVGIELEYPV +LEGDATDVEVIK LF YLVS LD TV
Sbjct: 1 MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEVIKDLFHYLVSTLDLTV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
KVDDFG IQLV+P SQD ILFEVSYTTIEFAFGKAETIQEVE RF+ YM+ IQ+KL E
Sbjct: 61 AKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQEVEERFSIYMEAIQKKLAE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
SNHAIVGCGIHPNW+KNEN PVAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQV
Sbjct: 121 SNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLD+SK+NYLRVINAFTQIE AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVG
Sbjct: 181 QLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VNARL DE DFFDYL+HSAIFTAERDGQTYYFYPIQA DYLAT EIQA+ALNGDE++I
Sbjct: 241 VNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPIQAGDYLATPEIQAFALNGDEVIIS 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA
Sbjct: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
FFK FG +YK LRRQFSKK LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+
Sbjct: 361 PFFKVFGYDYKSLRRQFSKKNLTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLR 420
Query: 421 EELGL 425
EEL L
Sbjct: 421 EELSL 425
>gi|111658751|ref|ZP_01409388.1| hypothetical protein SpneT_02000164 [Streptococcus pneumoniae
TIGR4]
Length = 404
Score = 697 bits (1800), Expect = 0.0, Method: Composition-based stats.
Identities = 348/404 (86%), Positives = 372/404 (92%)
Query: 22 LFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTVEKVDDFGTPIQLVEPASQDVI 81
+FVGIELEYPV +LEGDATDVEV+K LF YLVS LD TV KVDDFG IQLV+P SQD I
Sbjct: 1 MFVGIELEYPVASLEGDATDVEVMKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAI 60
Query: 82 LFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTP 141
LFEVSYTTIEFAFGKAETIQEVENRFNNYM+VIQRKL ESNHAIVGCGIHPNW+KNEN P
Sbjct: 61 LFEVSYTTIEFAFGKAETIQEVENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCP 120
Query: 142 VAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDVSKSNYLRVINAFTQIEG 201
VAYPRYQML+DYLNLSRN+ +SDLHHFPEYG FICGSQVQLD+SK+NYLRVINAFTQIE
Sbjct: 121 VAYPRYQMLMDYLNLSRNIIKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEA 180
Query: 202 AKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
AKAYL ANSEFSG DWDTKISRDIFWEESMHGIYPENVGVNARL DE DFFDYL+HSAI
Sbjct: 181 AKAYLFANSEFSGADWDTKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAI 240
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLTTRG 321
FTAERDGQTYYFYPIQA DYLAT+EIQA+ALNGDE++IYPQEKDFETHRSYQYQDLTTRG
Sbjct: 241 FTAERDGQTYYFYPIQAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRG 300
Query: 322 TVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFSKKK 381
TVEFRSVCTQPL+RTFASAAFHLGLLVNLDKLEAYLETA FFK FG +YK LRRQFSKK
Sbjct: 301 TVEFRSVCTQPLDRTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKN 360
Query: 382 LTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLKEELGL 425
LT+EEET IIEFSK +LLLA EGL +RNK+E +YLQPL+EEL L
Sbjct: 361 LTDEEETTIIEFSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL 404
>gi|157075124|gb|ABV09807.1| conserved hypothetical protein [Streptococcus gordonii str. Challis
substr. CH1]
Length = 426
Score = 490 bits (1261), Expect = e-137, Method: Composition-based stats.
Identities = 243/419 (57%), Positives = 312/419 (74%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
M+ +I LLK RYLKNIKENP ++VGIELE+P+VN +G+ATD+ + K L + L+ F V
Sbjct: 1 MTNAIKLLKDRYLKNIKENPTIYVGIELEFPIVNCQGEATDINIAKNLLKRLIDQHSFEV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
EK D G PIQ+ ++D ILFEVSY T+EFAF KA IQEVE RF Y+ VIQ LGE
Sbjct: 61 EKFDRAGNPIQIKSKENEDRILFEVSYNTLEFAFEKAAKIQEVEERFTEYLSVIQSILGE 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
+H + G GIHP W+KN+N+PV PRY+ML+ YL++S+ + LH +PEYGAFICG+Q+
Sbjct: 121 DDHELQGKGIHPFWDKNDNSPVQSPRYEMLMQYLSMSKTLELKTLHSYPEYGAFICGNQI 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLDVSK N++ V+N F QIE AKAYL ANSEF W+TKI+RDIFWE+SMHGI EN G
Sbjct: 181 QLDVSKENFISVVNVFNQIEAAKAYLFANSEFPDSSWNTKIARDIFWEQSMHGILQENAG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VN + F++ED+FF YL SAIFTA+R Q YYF+PI A DYL+ +I+AY L+G+ + +
Sbjct: 241 VNLKDFQNEDEFFSYLSKSAIFTAKRGNQDYYFFPIPAIDYLSHEKIKAYNLSGETVELT 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
P+++DFE HRSYQYQDLTTRGTVEFRSVC QP +RTF SAAFHLG+L NL+ ++ YL+ A
Sbjct: 301 PKQEDFENHRSYQYQDLTTRGTVEFRSVCAQPFDRTFISAAFHLGILANLENVQKYLQEA 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPL 419
FFK+ GRNYK LR++FSKKK++ T + F+ ++ LA GL RN E YL+ L
Sbjct: 361 DFFKKEGRNYKQLRQKFSKKKISRSAATHVRNFANDLIQLAIVGLVARNNGEEFYLKNL 419
>gi|24380341|ref|NP_722296.1| hypothetical protein SMU.1999c [Streptococcus mutans UA159]
gi|24378359|gb|AAN59602.1|AE015023_3 conserved hypothetical protein [Streptococcus mutans UA159]
Length = 428
Score = 474 bits (1221), Expect = e-132, Method: Composition-based stats.
Identities = 240/422 (56%), Positives = 309/422 (73%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTV 60
M++++DLLK RYL NI++ PE ++G+ELE+P+VNL ATD+ V K + +L +L F V
Sbjct: 1 MAKAVDLLKKRYLDNIQDKPESYIGVELEFPIVNLSHQATDITVTKSVMFHLKESLFFEV 60
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
E+ D G P+QL++ S+D ILFEVSY TIEFAFGKA+ IQ++E RFN YM VIQ L
Sbjct: 61 ERYDQDGCPVQLIDRKSEDRILFEVSYNTIEFAFGKAKKIQDIEERFNQYMKVIQAFLKP 120
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
NHAI G G+HP W N+N V PRYQML+++L LS N +S H FP+YG+FICG+QV
Sbjct: 121 YNHAIQGFGVHPYWYLNDNRAVKLPRYQMLLNFLALSGNKKDSFFHGFPQYGSFICGNQV 180
Query: 181 QLDVSKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVG 240
QLDVSKSNYLRVINAF +IE KAYL ANSEF G++W TKISRDIFWE SMHG++ ENVG
Sbjct: 181 QLDVSKSNYLRVINAFNKIEAVKAYLFANSEFWGQNWSTKISRDIFWENSMHGVFEENVG 240
Query: 241 VNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
VN F+ E+DFF+YL +SA+FTAER+ + YF PI+ +DYL EI A+ L+G + I
Sbjct: 241 VNKISFETEEDFFNYLANSALFTAERETEILYFEPIRVKDYLGQEEISAWDLSGQQHYIL 300
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
P E DF HRSYQYQ+LT RGTVEFRSVCTQPLE+TF AFH+GLL NLD+LE LE +
Sbjct: 301 PDEADFANHRSYQYQNLTKRGTVEFRSVCTQPLEKTFVPIAFHVGLLENLDELEKLLEKS 360
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
F + + +YKF+RRQFS+K +T E + I FS+ +L GL+ R E Y++ +K
Sbjct: 361 DFLQFYNHDYKFIRRQFSQKVITQEAKRFISVFSEAVLECCERGLKRRGLGEERYIKEIK 420
Query: 421 EE 422
++
Sbjct: 421 QK 422
>gi|84489637|ref|YP_447869.1| predicted glutamylcysteine synthetase [Methanosphaera stadtmanae
DSM 3091]
gi|84372956|gb|ABC57226.1| predicted glutamylcysteine synthetase [Methanosphaera stadtmanae
DSM 3091]
Length = 470
Score = 231 bits (589), Expect = 8e-59, Method: Composition-based stats.
Identities = 146/423 (34%), Positives = 221/423 (52%), Gaps = 7/423 (1%)
Query: 8 LKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVI-KYLFRYLVSALDFTVEKVDDF 66
L R++++ K N ++GIE+E P++NLE D +V+ K + +F + +D
Sbjct: 17 LYQRFIESTKNNENNYIGIEIEIPILNLEKKPVDFQVVHKITEEFKKQYTNFKPQTIDHN 76
Query: 67 GTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIV 126
L+ + D+I ++ SY +EF+FG+ + + RF +Y + + NH +
Sbjct: 77 NHICSLINKKNNDIISYDCSYNNLEFSFGRETNLFNIYERFCDYYSFFKELFEKHNHTLT 136
Query: 127 GCGIHPNWNKNENTPVAYPRYQMLVDYL-NLSR-NMAESDLHHFPEYGAFICGSQVQLDV 184
G GI+P N++ P+ RYQML +L + SR + H PEYG F SQ+QLDV
Sbjct: 137 GLGINPYRIYNKHIPIPNERYQMLYHHLCSYSRYSKLPKYFHDHPEYGMFSSASQIQLDV 196
Query: 185 SKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWEESMHGIYPENVGVNAR 244
+ N ++ IN F+++E KA L +NS GE+ D RD+FW++S HGI P N+G+
Sbjct: 197 NYDNLIKTINIFSKLEPIKAILFSNSVLLGENEDLLCCRDMFWQDSTHGINPHNIGMYET 256
Query: 245 LFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQA-YALNG--DEILIYP 301
KD DD Y++ I+ R+G F I DY ++ Y G D+I I P
Sbjct: 257 QLKDIDDLMAYIESLNIYCTMRNGLYVNFPTINIMDYFRKDSVEGEYCDKGVYDKITITP 316
Query: 302 QEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETAT 361
+ D E RS+++ DLT RGT+E+RS CTQP++ AF LGL L +LE E
Sbjct: 317 KISDIEYLRSFKFLDLTYRGTIEYRSSCTQPIKDVLCVGAFQLGLKNKLYELEEIFENDE 376
Query: 362 FFKEFGRNYKFLRRQFSKKKLTN-EEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLK 420
G N LR+ F K++L N +E + K IL L+ EGL R E +L+ L
Sbjct: 377 VIYHRGYNASELRKLFVKRQLPNFVDEDKLYTLIKQILDLSKEGLIERGYGEEVFLESLY 436
Query: 421 EEL 423
E +
Sbjct: 437 ERV 439
>gi|55821872|ref|YP_140314.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
gi|55823787|ref|YP_142228.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
gi|55737857|gb|AAV61499.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
gi|55739772|gb|AAV63413.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
Length = 201
Score = 202 bits (513), Expect = 5e-50, Method: Composition-based stats.
Identities = 101/194 (52%), Positives = 135/194 (69%)
Query: 231 MHGIYPENVGVNARLFKDEDDFFDYLDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAY 290
MHG++ EN GV ++F++EDD+F YL +AIFT R TYYF PI+A+DYL IQA+
Sbjct: 1 MHGVFEENGGVFPKVFENEDDYFSYLSETAIFTVTRGEVTYYFEPIRAKDYLNKPAIQAW 60
Query: 291 ALNGDEILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNL 350
+++G E+ I P E DF+THRSYQ+QDLTTRGTVEFRSVCTQP TFA AA HLGLLVNL
Sbjct: 61 SIHGKEVSIQPSEDDFQTHRSYQFQDLTTRGTVEFRSVCTQPFSATFAPAALHLGLLVNL 120
Query: 351 DKLEAYLETATFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNK 410
+ LE+ L+ + F+ F +Y +R FSKKK++ + I+ ++ +L A +GL+ R
Sbjct: 121 EALESILKGTSLFEVFDYDYPRIRCLFSKKKISKTDLKLILPLTEALLTCAEDGLKNRGF 180
Query: 411 QETSYLQPLKEELG 424
E YL PL+E L
Sbjct: 181 GEEVYLAPLQERLA 194
>gi|114567570|ref|YP_754724.1| Gamma-glutamylcysteine synthetase-like protein [Syntrophomonas
wolfei subsp. wolfei str. Goettingen]
gi|114338505|gb|ABI69353.1| Gamma-glutamylcysteine synthetase-like protein [Syntrophomonas
wolfei subsp. wolfei str. Goettingen]
Length = 479
Score = 119 bits (297), Expect = 5e-25, Method: Composition-based stats.
Identities = 108/413 (26%), Positives = 173/413 (41%), Gaps = 46/413 (11%)
Query: 24 VGIELEYPVVNLEGDATDVEVIKYLFRYLVSALDFTVEKVDDFGTPIQLVEPASQDVILF 83
+G+E+E PVVNL A + I + L FT + D G PI+ V QD F
Sbjct: 32 IGVEIEMPVVNLACRAVEPGFIAAMLDLLKQRFAFTPTRFDQNGFPIEAVN-LQQDAFSF 90
Query: 84 EVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVA 143
E ++ TIEFA K I ++ F +Y+ +Q E +H I G GI+P ++ P+
Sbjct: 91 ETTFNTIEFAMAKKPGIDQIAANFYSYVTALQELAHEHHHLICGMGINPYAAYADSKPLN 150
Query: 144 YPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDVSKSNYLRVINAFTQIEGAK 203
P ++L + + ++ H AF Q LDV + ++N ++
Sbjct: 151 SPVMLAKTEFLKNFTSHHDGEIFH-----AFSAACQTHLDVELTQLPDLLNLLGKLAFVD 205
Query: 204 AYLLANSE---FSG-EDWDTKIS--------------RDIFWEESMHGIYPENVGVNARL 245
A L ANS ++G ++ ++ RDI W NV R
Sbjct: 206 AMLFANSLPFIYAGNRNFTARLPASLQREMAHPTLCFRDILWRLCE----APNVRPYDRD 261
Query: 246 FKDEDDFFDYLDHSAIFTAERDGQTYY--FYPIQARDYLATTEIQAYALNGDEILIYPQE 303
+ ++ +L IF DG + + P++ DY+A E
Sbjct: 262 YASLEEVVKHLLDLKIFITS-DGSSGFKAIPPVRFADYIARDRWS--------------E 306
Query: 304 KDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFF 363
D RS + ++ GTVE R CTQPL +FA AAF+ G+ N+ +
Sbjct: 307 ADLSCFRSLEPVAVSRHGTVEIRQTCTQPLADSFAPAAFYTGIAENITVASELVHDFWQD 366
Query: 364 KEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYL 416
+ + LRRQ +++T + + EF ++L +A GL+ R + E YL
Sbjct: 367 NQINISNSQLRRQAVSREMTIPADR-LQEFINNLLAIAAAGLKKRKRGEDKYL 418
>gi|55821873|ref|YP_140315.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
gi|55823788|ref|YP_142229.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
gi|55737858|gb|AAV61500.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
gi|55739773|gb|AAV63414.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
Length = 62
Score = 79.3 bits (194), Expect = 4e-13, Method: Composition-based stats.
Identities = 38/61 (62%), Positives = 49/61 (80%)
Query: 149 MLVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDVSKSNYLRVINAFTQIEGAKAYLLA 208
ML+++L LS+ + + +PEYG+FICGSQVQLDVSK++Y RV+NAF QIEG KA LLA
Sbjct: 1 MLMNFLELSKAKNDPFFYDYPEYGSFICGSQVQLDVSKTSYSRVLNAFNQIEGPKAVLLA 60
Query: 209 N 209
N
Sbjct: 61 N 61
>gi|58040405|ref|YP_192369.1| Glutamate--cysteine ligase [Gluconobacter oxydans 621H]
gi|58002819|gb|AAW61713.1| Glutamate--cysteine ligase [Gluconobacter oxydans 621H]
Length = 486
Score = 67.8 bits (164), Expect = 1e-09, Method: Composition-based stats.
Identities = 85/359 (23%), Positives = 145/359 (40%), Gaps = 49/359 (13%)
Query: 73 VEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHP 132
+EPA Q FE+S + +IQE E + D ++ E + G P
Sbjct: 130 LEPAGQ----FELSGAPVV-------SIQETEAEMQAHFDQVRGPAAELGLGFLPFGFQP 178
Query: 133 NWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS 185
W ++ + RY ++ +Y+ P+ G+ C QV LD S
Sbjct: 179 LWPRDAMPWMPKSRYAIMRNYM--------------PKVGSLGLDMMTRTCTVQVNLDFS 224
Query: 186 -KSNYLRVINAFTQIEGAKAYLLANSEF-SGEDWDTKISRDIFWEESMHGIYPENVGVNA 243
+++ R + ++ L ANS F G+ +R W ++ + + G +
Sbjct: 225 DEADMARKMRVSLALQPVATALFANSPFVEGKANGLLSNRARIWTDTDN----QRSGQPS 280
Query: 244 RLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIY 300
F+D F Y+D + ++ RDG+ R +LA ++Q L G +
Sbjct: 281 VFFEDGFGFEQYVDWALDVPMYFVSRDGKNIDVAGCSFRRWLAG-DVQE-PLKG----LT 334
Query: 301 PQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETA 360
P DFE H + + D+ + +E R LE A +A +GLL + LEA +
Sbjct: 335 PTVGDFEDHLTTVFPDVRLKQFLEMRGADAGKLEMMVAQSALWVGLLYDPATLEAAEKLV 394
Query: 361 TFFKEFGRNYKFLRRQFSKKKLTNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPL 419
++ Y+ LR + + L + F+K ++ LA +GL R + E YL PL
Sbjct: 395 R--EQPWSVYQQLRAEVPRLGLDAAFPGGLKPFAKRVVELAEQGLRARVRNEAGYLDPL 451
>gi|149913419|ref|ZP_01901952.1| glutamate--cysteine ligase [Roseobacter sp. AzwK-3b]
gi|149812539|gb|EDM72368.1| glutamate--cysteine ligase [Roseobacter sp. AzwK-3b]
Length = 456
Score = 65.9 bits (159), Expect = 4e-09, Method: Composition-based stats.
Identities = 80/354 (22%), Positives = 149/354 (42%), Gaps = 46/354 (12%)
Query: 90 IEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQM 149
+E + ETI E + N ++ ++ + +G G P W+ + + RY++
Sbjct: 98 LELSGAPLETIHETCDEVNAHLRDVKDIADKVGVGFIGLGAAPIWSHEDMPRMPKGRYKL 157
Query: 150 LVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDV-SKSNYLRVINAFTQIEGAKAYLLA 208
+ DY++ M +S ++ C QV LD S+++ +R + ++ L A
Sbjct: 158 MTDYMDRVGTMGKSMMYR-------TCTVQVNLDFGSEADMVRKLRVALALQPVATALFA 210
Query: 209 NSEF-----SG-EDWDTKISRDIFWEESMHGIYP----ENVGVNARLFKDEDDFFDYLDH 258
NS F +G + W ++ RD+ + + G+ P E G A + DY
Sbjct: 211 NSPFFDGKPTGLKSWRARVWRDL--DNARTGMLPFVFDEGFGFEA--------WADYALD 260
Query: 259 SAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ RDG+ RD+L + AL G++ P D+ H + + +
Sbjct: 261 VPMYFVYRDGKYIDALGQSFRDFLKG---ELPALPGEK----PTLSDWADHLTTIFPEAR 313
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GL+ + L+A + + R + LR + S
Sbjct: 314 IKKYLEMRGADGGPWRRLCALPAFWVGLMYDQSSLDAAWDIVKDWDAETR--EALRIEAS 371
Query: 379 KKKL-TNEEETAIIEFSKHILLLACEGLEMRNK--------QETSYLQPLKEEL 423
+K L N ++ + ++ +L L+ GL+ R + ET +L LKE +
Sbjct: 372 EKALQANVMGISMHDLAREVLDLSEAGLKARARAGANGMIPDETHFLNALKESV 425
>gi|3913791|sp|O22493|GSH1_LYCES Glutamate--cysteine ligase, chloroplast precursor
(Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
gi|2407615|gb|AAB71230.1| gamma-glutamylcysteine synthetase [Lycopersicon esculentum]
Length = 523
Score = 64.7 bits (156), Expect = 1e-08, Method: Composition-based stats.
Identities = 77/345 (22%), Positives = 142/345 (41%), Gaps = 42/345 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + RY+++
Sbjct: 174 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEII 233
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 234 RNYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 279
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 280 ATALFANSPFTEGKPNGYLSKRSHIWTDTDN----NRAGMLPFVFDDSFGFEQYVDYAL- 334
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYA-LNGDEILI---YPQEKDFETHRSYQYQDL 317
D Y+ Y + + Y+ T + +NG I YP D+E H + + ++
Sbjct: 335 -----DVPMYFVY--RKKKYVDCTGLSFRDFMNGKLPPIPGEYPTLNDWENHLTTIFPEV 387
Query: 318 TTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQF 377
+ +E R P R A AF +G+L + L++ L+ F LR +
Sbjct: 388 RLKRYLEMRGADGGPWRRLCALPAFWVGILYDEGSLQSVLDMT--FDWTAEERDMLRNKV 445
Query: 378 SKKKL-TNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLKE 421
K L T + ++ ++ ++ LA EGLE R +ET +L + E
Sbjct: 446 PKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAE 490
>gi|99078054|ref|YP_611312.1| glutamate--cysteine ligase [Silicibacter sp. TM1040]
gi|99035192|gb|ABF62050.1| glutamate--cysteine ligase [Silicibacter sp. TM1040]
Length = 456
Score = 64.3 bits (155), Expect = 2e-08, Method: Composition-based stats.
Identities = 79/351 (22%), Positives = 144/351 (41%), Gaps = 40/351 (11%)
Query: 90 IEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQM 149
+E + ETI E + N ++ ++ E +G G P W +E + RYQ+
Sbjct: 98 LELSGAPLETIHETCDEVNTHLREVKDIADEIGVGFIGLGAAPIWQHDEMPLMPKGRYQL 157
Query: 150 LVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLD-VSKSNYLRVINAFTQIEGAKAYLLA 208
+ DY+ M + + C QV LD S+++ ++ + ++ L A
Sbjct: 158 MNDYMERVGTMGRAMMRR-------TCTVQVNLDFASEADMVQKLRVALALQPVATALFA 210
Query: 209 NSE-FSGEDWDTKISRDIFW---EESMHGIYPENVGVNARLFKDEDDFFDYLDHS---AI 261
NS F G+ K R W +++ G+ P +F++ F Y D++ +
Sbjct: 211 NSPFFEGKPNGLKSWRSHIWRHLDDARTGMLP-------FVFEEGMGFERYADYALDVPM 263
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLTTRG 321
+ RDG+ RD+L + AL G+ P D+ H + + + +
Sbjct: 264 YFVYRDGKYINALGQSFRDFLKG---ELPALPGET----PTLSDWADHLTTAFPEARIKK 316
Query: 322 TVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFSKKK 381
+E R P R A AF +GL+ N L+A + + R + LR + + K
Sbjct: 317 YMEMRGADGGPWRRLCALPAFWVGLMYNQSALDAAWDLVKGWDAETR--ENLRIEAAVKG 374
Query: 382 LTNE-EETAIIEFSKHILLLACEGLEMRNKQ--------ETSYLQPLKEEL 423
L E + + ++ +L ++ GL+ R +Q ET +L L+E +
Sbjct: 375 LQAEVGAIKMHDLAREVLAISEAGLKARARQGAGGLVPNETHFLNALRESV 425
>gi|150395612|ref|YP_001326079.1| glutamate--cysteine ligase [Sinorhizobium medicae WSM419]
gi|150027127|gb|ABR59244.1| glutamate--cysteine ligase [Sinorhizobium medicae WSM419]
Length = 457
Score = 63.5 bits (153), Expect = 2e-08, Method: Composition-based stats.
Identities = 83/381 (21%), Positives = 149/381 (39%), Gaps = 42/381 (11%)
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
E + D G I L EP+ I E E + E + + N ++ V++
Sbjct: 72 EPIMDEGNVIGLAEPSGNGAISLEPG-GQFELSGAPLENLHQTCKESNQHLAVLREIAEP 130
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
+G G P W E + RY ++ Y+ ++ C QV
Sbjct: 131 LGIRFLGIGGSPKWTFAETPRMPKSRYAIMTRYMPKVGTQGLDMMYR-------TCTIQV 183
Query: 181 QLDV-SKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPEN 238
LD S+ + R + +++ L A+S F+ + +S R W ++ + +
Sbjct: 184 NLDFSSEEDMRRKMQVSLKLQPLATALFASSPFTEGKPNGLLSWRGDIWRDTDN----QR 239
Query: 239 VGVNARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGD 295
G+ FK + F DY++ + ++ RDG + + R ++ AL G+
Sbjct: 240 AGLLPTAFKPDFGFGDYVEWALEVPMYFVVRDGHYHDCTHVTFRQFMNG------ALKGE 293
Query: 296 EILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEA 355
P D+ H S + D+ + +E R P R A AF +GLL + + L A
Sbjct: 294 IAEWQPTMGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYDDEALSA 353
Query: 356 YLETATFFKEFGRNYKF-----LRRQFSKKKLTNE-EETAIIEFSKHILLLACEGLEMRN 409
+E R++ + LR K L E ++ + ++ +L ++ GL+ RN
Sbjct: 354 A-------EELTRDWSYEEVLALRNAVPAKALAAEFRSKSLFDVAREVLAISRNGLKRRN 406
Query: 410 K------QETSYLQPLKEELG 424
+ E+ +L PL E L
Sbjct: 407 RLNGDGIDESQFLAPLDEVLA 427
>gi|3913792|sp|O23736|GSH1_BRAJU Glutamate--cysteine ligase, chloroplast precursor
(Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
gi|2243126|emb|CAA71801.1| gamma-glutamylcysteine synthetase [Brassica juncea]
gi|31043668|emb|CAD91712.1| glutamate-cysteine ligase [Brassica juncea]
Length = 514
Score = 63.2 bits (152), Expect = 3e-08, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 143/344 (41%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 165 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIM 224
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 225 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 270
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 271 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 326
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+G+ + R +LA + L G+ P D+E H + + ++
Sbjct: 327 VPMYFAYRNGKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 379
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L++ L+ + R + LR +
Sbjct: 380 LKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQSVLDLTADWTPAER--EMLRNKVP 437
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 438 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTE 481
>gi|110590457|pdb|2GWD|A Chain A, Crystal Structure Of Plant Glutamate Cysteine Ligase In
Complex With Mg2+ And L-Glutamate
Length = 449
Score = 62.4 bits (150), Expect = 5e-08, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 143/344 (41%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 100 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPTMPKGRYDIM 159
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 160 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 205
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 206 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 261
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+G+ + R +LA + L G+ P D+E H + + ++
Sbjct: 262 VPMYFAYRNGKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 314
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L++ L+ + R + LR +
Sbjct: 315 LKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQSVLDLTADWTPAER--EMLRNKVP 372
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 373 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEVGFLNAVTE 416
>gi|16226411|gb|AAL16161.1|AF428393_1 AT4g23100/F7H19_290 [Arabidopsis thaliana]
Length = 522
Score = 62.4 bits (150), Expect = 5e-08, Method: Composition-based stats.
Identities = 75/345 (21%), Positives = 139/345 (40%), Gaps = 42/345 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYAL- 333
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQE----KDFETHRSYQYQDL 317
D Y+ Y + Y+ T + A ++ P E D+E H + + ++
Sbjct: 334 -----DVPMYFAY--RKNKYIDCTGMTFSAFLAGKLPCLPGELPSYNDWENHLTTIFPEV 386
Query: 318 TTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQF 377
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 387 RLKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER--EMLRNKV 444
Query: 378 SKKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 445 PVTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|15912189|gb|AAL08228.1| AT4g23100/F7H19_290 [Arabidopsis thaliana]
Length = 522
Score = 62.0 bits (149), Expect = 7e-08, Method: Composition-based stats.
Identities = 76/344 (22%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 334
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + R +LA + L G+ P D+E H + + ++
Sbjct: 335 VPMYFAYRKNKYIDCTGMTFRQFLAG---KLPCLPGE----LPSYNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF LGLL + D L+A L+ + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWLGLLYDDDSLQAILDLTADWTPAER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 446 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|488615|emb|CAA82626.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
Length = 533
Score = 61.6 bits (148), Expect = 9e-08, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 334
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + R +LA + L G+ P D+E H + + ++
Sbjct: 335 VPMYFAYRKNKYIDCTGMTFRQFLAG---KLPCLPGE----LPSYNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 446 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|1742963|emb|CAA71075.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
Length = 522
Score = 60.8 bits (146), Expect = 1e-07, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 334
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + R +LA + L G+ P D+E H + + ++
Sbjct: 335 VPMYFAYRKNKYIDCTGMTFRQFLAG---KLPCLPGE----LPSYNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 446 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|15236390|ref|NP_194041.1| RML1 (PHYTOALEXIN DEFICIENT 2, ROOT MERISTEMLESS 1);
glutamate-cysteine ligase [Arabidopsis thaliana]
gi|12644273|sp|P46309|GSH1_ARATH Glutamate--cysteine ligase, chloroplast precursor
(Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
gi|16930445|gb|AAL31908.1|AF419576_1 AT4g23100/F7H19_290 [Arabidopsis thaliana]
gi|3292836|emb|CAA19826.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
gi|4262277|gb|AAD14544.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
gi|7269157|emb|CAB79265.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
gi|23506099|gb|AAN28909.1| At4g23100/F7H19_290 [Arabidopsis thaliana]
Length = 522
Score = 60.8 bits (146), Expect = 2e-07, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 334
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + R +LA + L G+ P D+E H + + ++
Sbjct: 335 VPMYFAYRKNKYIDCTGMTFRQFLAG---KLPCLPGE----LPSYNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 446 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|116295202|gb|ABJ98542.1| gamma-glutamylcysteine synthetase [Arabidopsis thaliana]
Length = 522
Score = 60.8 bits (146), Expect = 2e-07, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGTLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 334
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + R +LA + L G+ P D+E H + + ++
Sbjct: 335 VPMYFAYRKNKYIDCTGMTFRQFLAG---KLPCLPGE----LPSYNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDSLQAILDLTADWTPAER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 446 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVDE 489
>gi|34484367|gb|AAO45821.1| gamma-glutamylcysteine synthetase [Lotus corniculatus var.
japonicus]
gi|37681494|gb|AAO27827.1| gamma-glutamylcysteine synthetase [Lotus corniculatus var.
japonicus]
Length = 495
Score = 60.5 bits (145), Expect = 2e-07, Method: Composition-based stats.
Identities = 73/345 (21%), Positives = 140/345 (40%), Gaps = 42/345 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + RY+++
Sbjct: 146 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPIMPKGRYEIM 205
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 206 RNYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 251
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
L ANS F + +S R W ++ + G+ +F D F Y+D++
Sbjct: 252 ATALFANSPFKEGKPNGYVSMRSHIWTDTDK----DRSGMLPFVFDDSFGFEQYVDYAL- 306
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQE----KDFETHRSYQYQDL 317
D Y+ Y + Y+ T + ++ P E D+E H + + ++
Sbjct: 307 -----DVPMYFVY--RKHKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 359
Query: 318 TTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQF 377
+ +E R P R A AF +GLL + D L+ L+ + + R + LR +
Sbjct: 360 RLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQGVLDLTADWTQQER--QMLRNKV 417
Query: 378 SKKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
+ L +++ ++ +L LA +GLE R +E+ +L + E
Sbjct: 418 TVSGLKTPFRDGLLKHVAEDVLKLAKDGLEKRGFKESGFLNEVAE 462
>gi|15964523|ref|NP_384876.1| PUTATIVE GLUTAMATE--CYSTEINE LIGASE PRECURSOR PROTEIN
[Sinorhizobium meliloti 1021]
gi|15073700|emb|CAC45342.1| PUTATIVE GLUTAMATE--CYSTEINE LIGASE PRECURSOR PROTEIN
[Sinorhizobium meliloti]
Length = 481
Score = 60.5 bits (145), Expect = 2e-07, Method: Composition-based stats.
Identities = 82/381 (21%), Positives = 150/381 (39%), Gaps = 42/381 (11%)
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
E + D G I L EP+ I E E + E + + N ++ V++
Sbjct: 96 EPIMDEGNVIGLAEPSGNGAISLEPG-GQFELSGAPLENLHQTCKESNQHLAVLREIAEP 154
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
+G G P W+ E + RY ++ Y+ + ++ C QV
Sbjct: 155 LGIRFLGIGGSPKWSFAETPRMPKSRYAIMTRYMPKVGSKGLDMMYR-------TCTIQV 207
Query: 181 QLDV-SKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPEN 238
LD S+ + R + +++ L A+S F+ + +S R W ++ + +
Sbjct: 208 NLDFSSEEDMRRKMQVSLKLQPLATALFASSPFTEGKPNGLLSWRGDIWRDTDN----QR 263
Query: 239 VGVNARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGD 295
G+ FK + F DY++ + ++ R G + + R ++ AL G+
Sbjct: 264 SGLLPTAFKPDFGFSDYMEWALDVPMYFVVRGGHYHDCTHVTFRQFMNG------ALKGE 317
Query: 296 EILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEA 355
P D+ H S + D+ + +E R P R A AF +GLL + + L A
Sbjct: 318 IAEWQPTMGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYDDEALSA 377
Query: 356 YLETATFFKEFGRNYKF-----LRRQFSKKKLTNE-EETAIIEFSKHILLLACEGLEMRN 409
+E R++ + LR K L E ++ + ++ +L ++ GL+ RN
Sbjct: 378 A-------EELTRDWGYEEVLALRDAVPAKALAAEFRSRSLFDVAREVLTISRNGLKRRN 430
Query: 410 K------QETSYLQPLKEELG 424
+ E+ +L PL E L
Sbjct: 431 RLNGDGIDESQFLAPLDEVLA 451
>gi|100801746|emb|CAK24967.1| gamma-glutamylcysteine synthetase [Brassica napus]
Length = 514
Score = 60.5 bits (145), Expect = 2e-07, Method: Composition-based stats.
Identities = 75/344 (21%), Positives = 141/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 165 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPIMPKGRYDIM 224
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R ++
Sbjct: 225 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRVGLALQPI 270
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 271 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 326
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+ + + R +LA + L G+ P D+E H + + ++
Sbjct: 327 VPMYFAYRNNKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 379
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 380 LKRYMEMRGADGGPWRRLCALPAFWVGLLYDEDVLQAALDLTADWTPAER--EMLRNKVP 437
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L L E
Sbjct: 438 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNALTE 481
>gi|111380512|gb|ABD98695.2| chloroplast gamma-glutamylcysteine synthetase [Nicotiana tabacum]
Length = 522
Score = 60.5 bits (145), Expect = 2e-07, Method: Composition-based stats.
Identities = 74/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + RY+++
Sbjct: 173 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIM 232
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 233 RNYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 278
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 279 ATALFANSPFTEGKPNGYLSMRSHIWTDTDN----NRAGMLPFVFDDSFGFEQYVDYAL- 333
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILI---YPQEKDFETHRSYQYQDLT 318
D Y+ Y + A + + +NG I YP D+E H + + ++
Sbjct: 334 -----DVPMYFVYRKKKYIDCAGMSFRDF-MNGKLSPIPGDYPTLNDWENHLTTIFPEVR 387
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +G+L + L+ L+ + + R + LR +
Sbjct: 388 LKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQTVLDMTSDWTAEER--EMLRNKVP 445
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ ++ LA EGLE R +ET +L + E
Sbjct: 446 TSGLKTPFRDGLLKHVAQDVVKLAKEGLERRGYKETGFLNEVTE 489
>gi|126727777|ref|ZP_01743607.1| glutamate--cysteine ligase [Rhodobacterales bacterium HTCC2150]
gi|126702904|gb|EBA02007.1| glutamate--cysteine ligase [Rhodobacterales bacterium HTCC2150]
Length = 456
Score = 60.1 bits (144), Expect = 3e-07, Method: Composition-based stats.
Identities = 75/348 (21%), Positives = 146/348 (41%), Gaps = 34/348 (9%)
Query: 90 IEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQM 149
+E + ETI E + N ++ ++ +G G P W+ E + RY++
Sbjct: 98 LELSGAPLETIHETCDEVNVHLKEVKSVADRIGVRFIGLGGAPEWSHEEMAMMPKGRYRL 157
Query: 150 LVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLD-VSKSNYLRVINAFTQIEGAKAYLLA 208
+ DY+ M + ++ C QV LD S+++ ++ + ++ L A
Sbjct: 158 MTDYMGRVDTMGTTMMYR-------TCTVQVNLDFASEADMVQKLRVSLALQSVATALFA 210
Query: 209 NSEFS-GEDWDTKISRDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS---AIFTA 264
NS FS G+ K R W + + G+ +F++ F ++D++ ++
Sbjct: 211 NSPFSEGKPNGLKSFRSRVWRN----LDKDRTGIPGFVFEEGFGFQRWVDYALDVPMYFV 266
Query: 265 ERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLTTRGTVE 324
RDG+ RD+L + AL G++ P D+ H + + + + +E
Sbjct: 267 YRDGKYIDALGQSFRDFLRG---ELPALPGEK----PMLSDWADHLTTIFTEARIKQFIE 319
Query: 325 FRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFSKKKLTN 384
R P R A AF +GL+ + + L+A + + + R F R S++ L
Sbjct: 320 MRGADGGPWRRLCALPAFWVGLMYDQNSLDAASDLTKGW-DVERMEAF-RVAASEQGLQA 377
Query: 385 E-EETAIIEFSKHILLLACEGLEMRNK--------QETSYLQPLKEEL 423
E + +I+ ++ + ++ GL+ R + ET +L L+E L
Sbjct: 378 EVDGIKMIDLARECVNISEAGLKARARPGAGGLIPDETHFLNSLQESL 425
>gi|31043672|emb|CAD91714.1| glutamate-cysteine ligase [Brassica juncea]
Length = 515
Score = 60.1 bits (144), Expect = 3e-07, Method: Composition-based stats.
Identities = 74/344 (21%), Positives = 142/344 (41%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 166 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDMPIMPKGRYDIM 225
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R A ++
Sbjct: 226 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRAGLALQPI 271
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 272 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 327
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+ + + R +LA + L G+ P D+E H + + ++
Sbjct: 328 VPMYFAYRNKKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 380
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 381 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDVLQAVLDLIADWTPAER--EMLRNKVP 438
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GL+ R +E +L + E
Sbjct: 439 VTGLKTPFRDGLLKHVAEDVLKLAKDGLDRRGYKEAGFLNAVAE 482
>gi|120972552|gb|ABM46854.1| gamma-glutamylcysteine synthetase [Chorispora bungeana]
Length = 524
Score = 59.3 bits (142), Expect = 5e-07, Method: Composition-based stats.
Identities = 73/344 (21%), Positives = 142/344 (41%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 175 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWRREDIPIMPKGRYDIM 234
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ ++ A ++
Sbjct: 235 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIKKFRAGLALQPI 280
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
+ ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 281 ATAIFANSPFTEGKPNGFLSMRSQIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 336
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+ + + R +LA + L G+ P D+E H + + ++
Sbjct: 337 VPMYFAYRNKKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 389
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + D L+A L+ + R + LR +
Sbjct: 390 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDDDTLQAILDLTADWTAAER--EMLRNKVP 447
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 448 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVSE 491
>gi|118735071|ref|ZP_01583544.1| glutamate--cysteine ligase [Dinoroseobacter shibae DFL 12]
gi|118674819|gb|EAV81384.1| glutamate--cysteine ligase [Dinoroseobacter shibae DFL 12]
Length = 456
Score = 58.9 bits (141), Expect = 6e-07, Method: Composition-based stats.
Identities = 60/276 (21%), Positives = 114/276 (41%), Gaps = 33/276 (11%)
Query: 90 IEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQM 149
+E + TI E + N+++ +Q + +G G P W+ + + RY++
Sbjct: 98 LELSGAPLATIHETCDEVNSHLREVQDVAEKIGVGFIGLGAAPIWSHEDMPVMPKGRYRL 157
Query: 150 LVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLD-VSKSNYLRVINAFTQIEGAKAYLLA 208
+ DY++ M ++ ++ C QV LD S+++ ++ + ++ L A
Sbjct: 158 MTDYMDRVGTMGKTMMYR-------TCTVQVNLDFASEADMVQKLRVAFALQPVATALFA 210
Query: 209 NSEF------SGEDWDTKISRDIFWEESMHGIYPENVGVNARLFKDE---DDFFDYLDHS 259
NS F + W +++ RD + P+ G+ +F+D D + DY
Sbjct: 211 NSPFLDGKPNGHKSWRSRVWRD---------LDPDRTGMLPFVFEDGFGFDAWVDYALDV 261
Query: 260 AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLTT 319
++ RDGQ RD+L + + AL G+ P D+ H + + +
Sbjct: 262 PMYFVYRDGQYIDALGQSFRDFL---DGKLPALPGE----VPTLSDWADHLTTIFPEARI 314
Query: 320 RGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEA 355
+ +E R P R A AF +GL + L+A
Sbjct: 315 KKFIEMRGADGGPWRRLCALPAFWVGLTYDQGALDA 350
>gi|55823790|ref|YP_142231.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
gi|55739775|gb|AAV63416.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus CNRZ1066]
Length = 46
Score = 58.9 bits (141), Expect = 6e-07, Method: Composition-based stats.
Identities = 30/46 (65%), Positives = 37/46 (80%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIK 46
M+R+ +LLK RY K IK++PELFV IELEYPVVNL +ATDV + K
Sbjct: 1 MTRAKELLKQRYYKPIKDHPELFVEIELEYPVVNLSVNATDVSLSK 46
>gi|49475196|ref|YP_033237.1| Glutamate-cysteine ligase [Bartonella henselae str. Houston-1]
gi|49238001|emb|CAF27206.1| Glutamate-cysteine ligase [Bartonella henselae str. Houston-1]
Length = 457
Score = 58.5 bits (140), Expect = 7e-07, Method: Composition-based stats.
Identities = 88/396 (22%), Positives = 167/396 (42%), Gaps = 40/396 (10%)
Query: 45 IKYLFRYLVSALDFTVEKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVE 104
I+ L + AL + E + D G I LV Q I E E + +TI +
Sbjct: 58 IRALLEGMQKALGW--EPILDEGNIIGLVGSVGQGAISLEPG-GQFELSGALLKTIGDTY 114
Query: 105 NRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESD 164
++ ++++ +G G P W E + RY+++ +Y+ + S
Sbjct: 115 CEVIEHLALLKKISEPLGIGFLGIGASPKWTLTETPRMPKSRYRIMANYMP---KVGHSG 171
Query: 165 LHHFPEYGAFICGSQVQLDVS-KSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKIS- 222
L QV LD S +++ R + +++ L A+S F+ + +S
Sbjct: 172 LDMMYRTSTV----QVNLDFSSETDMRRKMQVSMKLQSIATALFASSPFTEGRPNGFLSW 227
Query: 223 RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQAR 279
R W ++ + + GV +F + F DY++ + ++ RDG+ Y I R
Sbjct: 228 RSEIWCDTDN----QRTGVLPFIFSERFGFADYVEWALDVPMYFVVRDGRYYDCTHITFR 283
Query: 280 DYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFAS 339
++ AL G P D+ H S + ++ + +E R L+R A
Sbjct: 284 QFMNG------ALRGQVANATPNMGDWINHLSTLFPEVRLKRFLEMRGADCGSLKRICAL 337
Query: 340 AAFHLGLLVN---LDKLEAYLETATFFKEFGRNYKFLRRQFSKKKL-TNEEETAIIEFSK 395
+AF +G+L + L++ EA + F + +R++ K+ L T +T I+E ++
Sbjct: 338 SAFWVGILYDSEALNEAEALTKDWCFEEVLD-----MRKRVPKEGLKTPFRQTIILEIAR 392
Query: 396 HILLLACEGLEMRNK------QETSYLQPLKEELGL 425
+ ++ +GL+ R + ETS+L PL+E + +
Sbjct: 393 QAVAISHKGLKNRRQYASNGLDETSFLAPLEEVIAM 428
>gi|18150411|gb|AAL61610.1|AF401621_1 gamma-glutamylcysteine synthetase [Allium cepa]
Length = 496
Score = 58.5 bits (140), Expect = 8e-07, Method: Composition-based stats.
Identities = 74/339 (21%), Positives = 142/339 (41%), Gaps = 30/339 (8%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + RY+++
Sbjct: 147 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGAGFQPKWGLKDIPIMPKGRYEIM 206
Query: 151 VDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDVS-KSNYLRVINAFTQIEGAKAYLLAN 209
+Y+ +M + C QV LD S +S+ +R A ++ + AN
Sbjct: 207 RNYMPKVGSMGLDMMFR-------TCTVQVNLDFSSESDMVRKFRAGLSLQPIATAIFAN 259
Query: 210 SEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAIFTAERDG 268
S F+ + +S R W ++ + G+ +FKD F Y+D++ D
Sbjct: 260 SPFTEGKPNGFLSKRSHIWTDTDN----NRSGMLPFVFKDSFGFEQYVDYAL------DV 309
Query: 269 QTYYFYPIQARDYLATTEIQAYALNGDEILIYPQE----KDFETHRSYQYQDLTTRGTVE 324
Y+ Y + + Y+ + + ++ P E D+E H + + ++ + +E
Sbjct: 310 PMYFVY--RNKKYIDCSGLSFRDFMKGKLSPVPGELPTLNDWENHLTTIFPEVRLKRYLE 367
Query: 325 FRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLE-TATFFKEFGRNYKFLRRQFSKKKLT 383
R P R A AF +GLL + L++ +E TA + +E LRR+ L
Sbjct: 368 MRGADGGPWRRLCALPAFWVGLLYDDIALQSVIEITADWTRE---ERDMLRRKVPVTGLK 424
Query: 384 NEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
+++ ++ +L LA GLE R +E +L+ + E
Sbjct: 425 TPFRDGLLKHVAEDVLKLAQGGLERRGYKEVGFLREVAE 463
>gi|50058088|dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]
Length = 523
Score = 57.8 bits (138), Expect = 1e-06, Method: Composition-based stats.
Identities = 73/345 (21%), Positives = 145/345 (42%), Gaps = 42/345 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY+++
Sbjct: 174 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFIGIGFQPKWERKDIPIMPKGRYEIM 233
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDV-SKSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S+++ +R A ++
Sbjct: 234 RNYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 279
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + + G+ +F D F Y++++
Sbjct: 280 ATALFANSPFTEGKPNGYLSMRSQIWTDTDN----DRSGMLPFVFDDSFGFEQYVEYALD 335
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ R + + RD+LA + + G+ YP D+E H + + ++
Sbjct: 336 VPMYFVYRKKKYIDCAGLSFRDFLAG---KLPPIPGE----YPTLNDWENHLTTIFPEVR 388
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLE-TATFFKEFGRNYKFLRRQF 377
+ +E R P R A AF +G+L + L+ L+ TA + +E + LR +
Sbjct: 389 LKRYLEMRGADGGPWRRLCALPAFWVGVLYDDISLQNVLDMTADWTQE---ERQMLRNKV 445
Query: 378 SKKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L A +GLE R +ET +L + E
Sbjct: 446 PVAGLKTPFRDGLLKHVAEEVLKFAKDGLERRGYKETGFLNEVAE 490
>gi|114705940|ref|ZP_01438843.1| glutamate-cysteine ligase protein [Fulvimarina pelagi HTCC2506]
gi|114538786|gb|EAU41907.1| glutamate-cysteine ligase protein [Fulvimarina pelagi HTCC2506]
Length = 457
Score = 57.8 bits (138), Expect = 1e-06, Method: Composition-based stats.
Identities = 95/381 (24%), Positives = 155/381 (40%), Gaps = 65/381 (17%)
Query: 64 DDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNH 123
D+ G I L EP Q FE+S ++ TI E N ++ ++ E
Sbjct: 86 DEEGGAISL-EPGGQ----FELSGAPLK-------TIHETCRESNKHLAEVRSIAKELGI 133
Query: 124 AIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGA-------FIC 176
+G G P W +E + RY ++ Y+ PE G
Sbjct: 134 GFLGIGSSPTWTLDETPIMPKSRYDIMRAYM--------------PEVGTRGLDMMLRTT 179
Query: 177 GSQVQLDVS-KSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKISRDIFWE-ESMHGI 234
QV LD S +++ R + +++ + L ANS F+ + K + + W E H +
Sbjct: 180 TIQVNLDFSDEADMRRKMQIGMKLQPVASALFANSPFT----EGKPNGLVSWRSEVWHDV 235
Query: 235 YPENVGVNARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYA 291
+ G+ +F+D + DY++ + ++ R G + R +L A
Sbjct: 236 DNDRSGLLPFVFEDGFTYRDYVEWALDVPMYFVSRGGHYNRATDVTFRQFLDG------A 289
Query: 292 LNGDEILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLV--- 348
LNG P D+ H S + D+ + +E R P R A AF +GLL
Sbjct: 290 LNGRFADAEPNMGDWTNHLSTLFPDVRLKRFIEMRGADGGPWRRICALPAFWVGLLYAPG 349
Query: 349 NLDKLEAYLETATF-FKEFGRNYKFLRRQFSKKKL-TNEEETAIIEFSKHILLLACEGLE 406
LDK+E TA F F+E LRR+ K+ T + +++ ++ + +A EGL
Sbjct: 350 ALDKVEEM--TADFTFEEISE----LRRRVPKEGFRTPFRDGTVLDVARECVRIAREGLI 403
Query: 407 MR-NKQ-----ETSYLQPLKE 421
R NK E+ +L PL+E
Sbjct: 404 DRANKNDEGNDESFFLAPLEE 424
>gi|31043670|emb|CAD91713.1| glutamate-cysteine ligase [Brassica juncea]
Length = 511
Score = 57.8 bits (138), Expect = 2e-06, Method: Composition-based stats.
Identities = 73/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 162 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPIMPKGRYDIM 221
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R ++
Sbjct: 222 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRVGLALQPI 267
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 268 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 323
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+ + + R +LA + L G+ P D+E H + + ++
Sbjct: 324 VPMYFAYRNNKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 376
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GL + D L+A L+ + R + LR +
Sbjct: 377 LKRYMEMRGADGGPWRRLCALPAFWVGLFYDEDVLQAALDLTADWTPAER--EMLRNKVP 434
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 435 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVTE 478
>gi|6651031|gb|AAF22137.1|AF128455_1 gamma-glutamylcysteine synthetase precursor [Pisum sativum]
Length = 499
Score = 57.4 bits (137), Expect = 2e-06, Method: Composition-based stats.
Identities = 75/345 (21%), Positives = 144/345 (41%), Gaps = 42/345 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY+++
Sbjct: 150 ELSGAPLETLHQTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWERKDIPMMPKGRYEIM 209
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDV-SKSNYLRVINAFTQIEGA 202
Y+ P+ G+ C QV LD S+++ +R A ++
Sbjct: 210 KKYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 255
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 256 ATALFANSPFTEGKPNGFVSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 311
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R + + RD+LA + + G+ P D+E H + + ++
Sbjct: 312 VPMYFAYRKKKYVDCTGMTFRDFLAG---KLPCIPGE----LPTLNDWENHLTTIFPEVR 364
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLE-TATFFKEFGRNYKFLRRQF 377
+ +E R P R A AF +GLL + L+ L+ TA + E + LR +
Sbjct: 365 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQRVLDMTADWTLE---EREMLRNKV 421
Query: 378 SKKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
+ L +++ ++ +L LA +GLE R +E+ +L + E
Sbjct: 422 TVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAE 466
>gi|121602200|ref|YP_988623.1| glutamate--cysteine ligase [Bartonella bacilliformis KC583]
gi|120614377|gb|ABM44978.1| glutamate--cysteine ligase [Bartonella bacilliformis KC583]
Length = 457
Score = 57.0 bits (136), Expect = 2e-06, Method: Composition-based stats.
Identities = 82/370 (22%), Positives = 151/370 (40%), Gaps = 34/370 (9%)
Query: 65 DFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHA 124
D G I LV Q I E E + K +T+ ++D++++
Sbjct: 76 DEGNIIGLVGSEGQGAISLEPG-GQFELSGAKLKTVGHTYCEVMEHLDLLKKISQPLGIG 134
Query: 125 IVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDV 184
+G G P W E + RY+++ +Y+ HH + QV LD
Sbjct: 135 FLGMGASPKWTLPETPQMPKSRYKIMTNYM-------PKVGHHGLDMMYRTATIQVNLDF 187
Query: 185 -SKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVN 242
S+++ R + +++ L A+S F+ + +S R W G + G+
Sbjct: 188 SSETDMRRKMQVSMKLQPIVTALFASSPFTEGRPNGFLSWRSAIW----CGTDNQRTGIL 243
Query: 243 ARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILI 299
+F D F DY++ + ++ RDG + + R ++ AL G I
Sbjct: 244 PFIFSDRFGFADYVEWALGVPMYFIIRDGHYHDCTHVTFRQFMNG------ALKGQIIDS 297
Query: 300 YPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLET 359
P D+ H S + ++ + +E R +R A +AF +GLL + EA E
Sbjct: 298 TPTMGDWVDHLSTLFPEVRLKRFLEMRGADGGSWQRICALSAFWVGLLYD---SEALSEA 354
Query: 360 ATFFKEFGRNYKF-LRRQFSKKKL-TNEEETAIIEFSKHILLLACEGLEMRNK------Q 411
K++ +R + +K+ + T +T I+E + + ++ +GL+ R +
Sbjct: 355 EALTKDWCYEEVLDMRNRVAKEGIKTPFRQTTILELASQAIAISRKGLKSRKQYGSDGFD 414
Query: 412 ETSYLQPLKE 421
ET++L PL+E
Sbjct: 415 ETNFLDPLEE 424
>gi|3688156|emb|CAA06613.1| gamma-glutamylcysteine synthetase [Brassica juncea]
Length = 436
Score = 57.0 bits (136), Expect = 3e-06, Method: Composition-based stats.
Identities = 73/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY ++
Sbjct: 87 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGMGFQPKWRREDIPIMPKGRYDIM 146
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G+ C QV LD S +++ +R ++
Sbjct: 147 RNYM--------------PKVGSLGLDMMLRTCTVQVNLDFSSEADMIRKFRVGLALQPI 192
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
L ANS F+ + +S R W ++ + G+ +F D F Y+D++
Sbjct: 193 ATALFANSPFTEGKPNGFLSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDYALD 248
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ A R+ + + R +LA + L G+ P D+E H + + ++
Sbjct: 249 VPMYFAYRNNKYVDCTGMTFRQFLAG---KLPCLPGE----LPTYNDWENHLTTIFPEVR 301
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GL + D L+A L+ + R + LR +
Sbjct: 302 LKRYMEMRGADGGPWRRLCALPAFWVGLFYDEDVLQAALDLTADWTPAER--EMLRNKVP 359
Query: 379 KKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
L +++ ++ +L LA +GLE R +E +L + E
Sbjct: 360 VTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEAGFLNAVTE 403
>gi|55821875|ref|YP_140317.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
gi|55737860|gb|AAV61502.1| glutamate--cysteine ligase, putative, truncated [Streptococcus
thermophilus LMG 18311]
Length = 61
Score = 56.6 bits (135), Expect = 3e-06, Method: Composition-based stats.
Identities = 29/46 (63%), Positives = 37/46 (80%)
Query: 1 MSRSIDLLKHRYLKNIKENPELFVGIELEYPVVNLEGDATDVEVIK 46
++R+ +LLK RY K IK++PELFV IELEYPVVNL +ATDV + K
Sbjct: 16 VTRAKELLKQRYYKPIKDHPELFVEIELEYPVVNLSVNATDVSLSK 61
>gi|86356449|ref|YP_468341.1| glutamate-cysteine ligase protein [Rhizobium etli CFN 42]
gi|86280551|gb|ABC89614.1| glutamate-cysteine ligase protein [Rhizobium etli CFN 42]
Length = 457
Score = 56.2 bits (134), Expect = 4e-06, Method: Composition-based stats.
Identities = 87/382 (22%), Positives = 147/382 (38%), Gaps = 44/382 (11%)
Query: 61 EKVDDFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGE 120
E + D G I L E I E E + ETI + N ++ ++
Sbjct: 72 EPILDGGNIIGLAEQNGMGAISIEPG-GQFELSGAPLETIHQTCRESNQHLATLREIAEP 130
Query: 121 SNHAIVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQV 180
+G G P W E + RY+++ Y+ ++ C QV
Sbjct: 131 MGIRFLGIGGSPKWTYAETPQMPKSRYEIMTRYMPKVGTKGLDMMYR-------TCTIQV 183
Query: 181 QLDVSKSNYLRV-INAFTQIEGAKAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPEN 238
LD S +R + +++ L A+S F+ + +S R W ++ +
Sbjct: 184 NLDFSSEADMRKKMRVSMKLQSLATALFASSPFTEGKPNGLLSWRGDIWRDTDN----RR 239
Query: 239 VGVNARLFKDEDDFFDY----LDHSAIFTAERDGQTYYFYPIQARDYLATTEIQAYALNG 294
G+ F+D+ F DY LD F RDG+ + + R ++ AL G
Sbjct: 240 SGLLDFTFRDDFGFRDYAEWALDVPMYFIV-RDGRYHDCTHVTFRQFMNG------ALKG 292
Query: 295 DEILIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLE 354
+ + P D+ H S + D+ + +E R P R A AF +GLL +
Sbjct: 293 EVVDPAPTMGDWTNHLSTLFPDVRLKRFLEMRGADGGPWRRICALPAFWVGLLYE----D 348
Query: 355 AYLETATFFKEFGRNYKF-----LRRQFSKKKLTNE-EETAIIEFSKHILLLACEGLEMR 408
A LE A E +++ F LR + L E +++E ++ ++ ++ GL+ R
Sbjct: 349 AALEAA---DELTKDWSFAEVSALRNAVPAEGLQAEFRGHSLLEMAREVVGISKAGLKSR 405
Query: 409 NK------QETSYLQPLKEELG 424
K E+ +L PL E L
Sbjct: 406 GKLNGEGQDESIFLAPLDEVLA 427
>gi|49473946|ref|YP_031988.1| Glutamate-cysteine ligase [Bartonella quintana str. Toulouse]
gi|49239449|emb|CAF25799.1| Glutamate-cysteine ligase [Bartonella quintana str. Toulouse]
Length = 457
Score = 55.8 bits (133), Expect = 5e-06, Method: Composition-based stats.
Identities = 84/374 (22%), Positives = 153/374 (40%), Gaps = 34/374 (9%)
Query: 65 DFGTPIQLVEPASQDVILFEVSYTTIEFAFGKAETIQEVENRFNNYMDVIQRKLGESNHA 124
D G I LV Q I E E + +TI ++ ++++ G
Sbjct: 76 DEGNIIGLVGSVDQGAISLEPG-GQFELSGAPLKTIGHTYCEVMEHLALLKKISGPLGIG 134
Query: 125 IVGCGIHPNWNKNENTPVAYPRYQMLVDYLNLSRNMAESDLHHFPEYGAFICGSQVQLDV 184
+G G P W E + RY+++ +Y+ + S L QV LD
Sbjct: 135 FLGIGASPKWTLAETPRMPKSRYRIMANYMP---KVGYSGLDMMYRTSTV----QVNLDF 187
Query: 185 -SKSNYLRVINAFTQIEGAKAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVN 242
S+++ R + +++ L A+S F+ + +S R W ++ + GV
Sbjct: 188 SSETDMRRKMQVSMKLQSIATALFASSPFTEGRPNGFLSWRSEVWCDTDK----QRTGVL 243
Query: 243 ARLFKDEDDFFDYLDHS---AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILI 299
+F + F DY++ + ++ RDG Y I R ++ AL G
Sbjct: 244 PFIFSEHFGFADYVEWALDVPMYFVVRDGHYYDCTHITFRQFMNG------ALKGKIANS 297
Query: 300 YPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLET 359
P D+ H S + ++ + +E R +R A +AF +GLL + EA E
Sbjct: 298 MPTMGDWINHLSTLFPEVRLKRFLEMRGADCGSWKRICALSAFWVGLLYD---SEALNEA 354
Query: 360 ATFFKEFGRNYKF-LRRQFSKKKLTNE-EETAIIEFSKHILLLACEGLEMRNK------Q 411
T K++ LR++ K+ L +T I+E ++ + ++ +GL+ R +
Sbjct: 355 ETLTKDWCFEEVLDLRKRVPKEGLRAPFRQTVILELARQAVAISRKGLKNRRQYDSDGFD 414
Query: 412 ETSYLQPLKEELGL 425
ET++L PL+E + +
Sbjct: 415 ETNFLTPLEEVIAM 428
>gi|57903694|gb|AAW58147.1| gamma-glutamylcysteine synthetase [Triticum aestivum]
Length = 374
Score = 55.8 bits (133), Expect = 5e-06, Method: Composition-based stats.
Identities = 75/346 (21%), Positives = 142/346 (41%), Gaps = 44/346 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + RY+++
Sbjct: 25 ELSGAPLETLHQTCAEVNSHLYQVKAVGEELGVGFLGMGFQPKWALADIPIMPKGRYEIM 84
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S + + +R A ++
Sbjct: 85 RNYM--------------PKVGTLGLDMMFRTCTVQVNLDFSSEQDMIRKFRASLALQPI 130
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
+ ANS F + +S R W ++ + G+ +F D F Y+D++
Sbjct: 131 ATAIFANSPFKEGKPNGFLSLRSHIWTDTDN----NRSGMLPFVFDDSFGFEQYVDYAL- 185
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQE----KDFETHRSYQYQDL 317
D Y+ Y + + Y+ T + ++ P E D+E H + + ++
Sbjct: 186 -----DVPMYFVY--RNKTYIDCTGMSFRDFMAGKLPXVPGELPTLNDWENHLTTIFPEV 238
Query: 318 TTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLE-TATFFKEFGRNYKFLRRQ 376
+ +E R P R A AF +GLL + + L++ ++ TA + KE + LRR+
Sbjct: 239 RLKRYMEMRGADGGPWRRLCALPAFWVGLLYDDESLQSIIDMTADWTKE---EREMLRRK 295
Query: 377 FSKKKL-TNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLKE 421
L T + + + ++ +L LA GLE R +E +L+ + E
Sbjct: 296 VPVTGLKTPFRDGYVRDLAEDVLQLAKNGLERRGYKEVGFLREVDE 341
>gi|11386873|sp|Q9ZNX6|GSH1_MEDTR Glutamate--cysteine ligase, chloroplast precursor
(Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS)
gi|3941322|gb|AAC82334.1| gamma-glutamylcysteine synthetase [Medicago truncatula]
Length = 508
Score = 55.5 bits (132), Expect = 7e-06, Method: Composition-based stats.
Identities = 73/346 (21%), Positives = 141/346 (40%), Gaps = 44/346 (12%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W + + + RY+++
Sbjct: 159 ELSGAPLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPMMPKGRYEIM 218
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDV-SKSNYLRVINAFTQIEGA 202
Y+ P+ G+ C QV LD S+++ +R A ++
Sbjct: 219 KKYM--------------PKVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPI 264
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHSAI 261
L ANS F+ + +S R W ++ + G+ +F D F Y+D +
Sbjct: 265 ATALFANSPFTDGKPNGFVSMRSHIWTDTDK----DRTGMLPFVFDDSFGFEQYVDFAL- 319
Query: 262 FTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQE----KDFETHRSYQYQDL 317
D Y+ Y + + Y+ T + ++ P E D+E H + + ++
Sbjct: 320 -----DVPMYFVY--RKKKYIDCTGMTFRDFLAGKLPCIPGELPTLNDWENHLTTIFPEV 372
Query: 318 TTRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLE-TATFFKEFGRNYKFLRRQ 376
+ +E R P R A AF +G+L + L+ L+ TA + E + LR +
Sbjct: 373 RLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQRVLDMTADWTLE---EREMLRNK 429
Query: 377 FSKKKLTNEEETAIIE-FSKHILLLACEGLEMRNKQETSYLQPLKE 421
+ L +++ ++ +L LA +GLE R +E+ +L + E
Sbjct: 430 VTVTGLKTPFRDGLLKHVAEEVLELAKDGLERRGFKESGFLNAVAE 475
>gi|17154688|emb|CAC83005.1| putative gamma-glutamylcysteine synthetase [Zea mays]
Length = 438
Score = 55.1 bits (131), Expect = 9e-06, Method: Composition-based stats.
Identities = 73/344 (21%), Positives = 140/344 (40%), Gaps = 40/344 (11%)
Query: 91 EFAFGKAETIQEVENRFNNYMDVIQRKLGESNHAIVGCGIHPNWNKNENTPVAYPRYQML 150
E + ET+ + N+++ ++ E +G G P W ++ + RY+++
Sbjct: 89 ELSGAPLETLHQTCAEVNSHLYQVKAVGEEMGIGFLGLGFQPKWALSDIPIMPKGRYEIM 148
Query: 151 VDYLNLSRNMAESDLHHFPEYGAF-------ICGSQVQLDVS-KSNYLRVINAFTQIEGA 202
+Y+ P+ G C QV LD S + + +R A ++
Sbjct: 149 RNYM--------------PKVGTLGLDMMFRTCTVQVNLDFSSEQDMIRKFRAGLALQPI 194
Query: 203 KAYLLANSEFSGEDWDTKIS-RDIFWEESMHGIYPENVGVNARLFKDEDDFFDYLDHS-- 259
+ ANS F + +S R W ++ + G+ +F D F Y+D++
Sbjct: 195 ATAIFANSPFKEGKPNGFLSLRSHIWTDTDN----NRAGMLPFVFDDSFGFEQYVDYALE 250
Query: 260 -AIFTAERDGQTYYFYPIQARDYLATTEIQAYALNGDEILIYPQEKDFETHRSYQYQDLT 318
++ R+ + + RD++ QA G+ P D+E H + + ++
Sbjct: 251 VPMYFVYRNKKYIDCTGMSFRDFMQGKLPQA---PGE----LPTLTDWENHLTTIFPEVR 303
Query: 319 TRGTVEFRSVCTQPLERTFASAAFHLGLLVNLDKLEAYLETATFFKEFGRNYKFLRRQFS 378
+ +E R P R A AF +GLL + + L++ L+ F + LRR+
Sbjct: 304 LKRYLEMRGADGGPWRRLCALPAFWVGLLYDEESLQSILDMT--FDWTKEEREMLRRKVP 361
Query: 379 KKKL-TNEEETAIIEFSKHILLLACEGLEMRNKQETSYLQPLKE 421
L T + + + ++ +L LA GLE R +E +L+ + E
Sbjct: 362 STGLKTPFRDGYVRDLAEEVLKLAKNGLERRGYKEVGFLREVDE 405
Database: nr
Posted date: Sep 17, 2007 11:41 AM
Number of letters in database: 999,999,834
Number of sequences in database: 2,976,859
Database: /nucleus1/users/jsaw/ncbi/db/nr.01
Posted date: Sep 17, 2007 11:48 AM
Number of letters in database: 894,087,890
Number of sequences in database: 2,493,262
Lambda K H
0.320 0.137 0.398
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,551,786,419
Number of Sequences: 5470121
Number of extensions: 66286580
Number of successful extensions: 141460
Number of sequences better than 1.0e-05: 54
Number of HSP's better than 0.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 39
Number of HSP's that attempted gapping in prelim test: 141404
Number of HSP's gapped (non-prelim): 55
length of query: 425
length of database: 1,894,087,724
effective HSP length: 136
effective length of query: 289
effective length of database: 1,150,151,268
effective search space: 332393716452
effective search space used: 332393716452
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)