Liu Di 1,
Zhang Xiao-mei 1, Hua Xin 1,
Qiao Nan 1, Song Han 1,
Lu Hai 1, Guo Hui-hong 1 and
Li Feng-lan 1 
| (1) | College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P. R. China |
Received: 22 October 2006 Accepted: 18 January 2007
Abstract Telomeres are the
structures that locate at the terminals of linear eukaryotic
chromosomes. They can play essential roles in many cellular processes.
The terminal location of Arabidopsis-type TTTAGGG tandem
repeats were thought to be highly conserved. The terminal location of Ginkgo
biloba L. consisting of TTTAGGG tandem repeats, were confirmed by Bal31
exonuclease degradation and Southern blotting. By comparing telomeric
restriction fragment (TRF) lengths at different developmental stages
from embryos to seedlings, a fluctuant tendency towards variation was
found in these samples. The TRF length of embryos was also compared
with that of embryonal calli and an upward trend was discovered in
callus culture. The results suggest that there should be a telomerase
mechanism or/and ALT mechanism for the maintenance of telomere length.
Key words Ginkgo biloba L. - maintenance mechanism - telometic restriction fragment length
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Li Feng-lan Email: lifl@bjfu.edu.cn |