RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Drum ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sciaenops ocellatus
FDA Seafood List Common Name: Red Drum
FDA Seafood List Market Name: Drum (Redfish)
Unique IEF Gel Code: 19970205gel02
Unique Web Code: drh01Srr
Tissue Aliquot Code: sciaocel K96-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	89	10.6	0.0506084	4	0.376486	5	3.6	4.55	89.4	4.3	0.0498559
2	5.2	184	22	0.0631852	5	0.51088	6.8	4.9	5.2	184.1	4.6	0.0632357
3	5.85	305	36.5	0.0424197	4	0.271161	3.6	2.6	5.85	304.5	3.8	0.0404093
4	6.55	447	53.5	0.125455	6	1.17818	15.7	11.4	6.55	446.3	5.7	0.116321
5	6.85	481	57.5	0.0210986	6	0.185347	2.5	1.8	6.85	481.1	5.7	0.0184746
6	7.35	546	65.3	0.0488591	4	0.345223	4.6	3.3	7.35	545.5	4.1	0.0479562
7	8.15	670	80.1	0.0719211	11	1.37333	18.3	13.3	8.15	668.7	10.8	0.0716711
8	8.45	729	87.2	0.127642	8	1.76426	23.5	17.1	8.45	728.3	7.9	0.126289
9	8.65	777	92.9	0.100067	4	0.72305	9.6	7	8.65	776.4	4.2	0.096589
10	9.3	819	98	0.0699927	7	0.770825	10.3	7.5	9.3	817.7	7.3	0.0594643

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.073	17	2	0.0516562	5	0.445623	1	0.9	4.073	16.5	5.1	0.049726
2	4.228	40	4.8	0.0481536	6	0.46911	1.1	1	4.228	39.6	5.7	0.04606
3	4.377	62	7.4	0.0888281	5	0.842463	1.9	1.7	4.377	61.8	5.5	0.0871084
4	4.479	77	9.2	0.579969	4	4.41674	9.9	9	4.479	77.3	4.2	0.586971
5	4.77	120	14.3	0.0228183	9	0.328034	0.7	0.7	4.77	119.5	8.6	0.0214144
6	5.109	170	20.3	0.0314502	4	0.220145	0.5	0.4	5.109	170.9	3.8	0.0324787
7	5.279	198	23.7	0.0349571	3	0.180904	0.4	0.4	5.279	197.4	2.9	0.0346924
8	5.344	210	25.1	0.47822	3	2.39846	5.4	4.9	5.344	210.6	2.9	0.468829
9	5.469	233	27.8	0.0222252	4	0.153179	0.3	0.3	5.469	232.5	3.8	0.0225674
10	5.6	257	30.7	0.090894	8	1.18304	2.7	2.4	5.6	256.9	7.6	0.0873684
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.73	281	33.6	0.0398881	4	0.28349	0.6	0.6	5.73	282	4	0.0395134
12	5.864	306	36.6	0.588696	3	3.37213	7.6	6.9	5.864	305.9	3.3	0.579795
13	5.951	324	38.7	0.251978	3	1.48311	3.3	3	5.951	323.9	3.4	0.245508
14	6.071	349	41.7	0.160273	5	1.30046	2.9	2.6	6.071	348.2	4.8	0.151468
15	6.139	363	43.4	0.226605	4	1.5812	3.6	3.2	6.139	362.6	4	0.2237
16	6.201	376	44.9	0.0487904	3	0.295861	0.7	0.6	6.201	377.3	2.9	0.057711
17	6.23	382	45.6	0.0883288	2	0.292088	0.7	0.6	6.23	381.9	1.9	0.0852095
18	6.273	391	46.7	0.0194837	1	0.0517599	0.1	0.1	6.273	391.1	1.5	0.0194824
19	6.374	412	49.2	0.0216731	2	0.0679964	0.2	0.1	6.374	412.3	1.6	0.0242249
20	6.403	418	49.9	0.145044	2	0.348291	0.8	0.7	6.403	418.3	1.6	0.123904
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.427	423	50.5	0.127384	4	0.835704	1.9	1.7	6.427	423	4	0.117713
22	6.466	431	51.5	0.578042	2	2.23953	5	4.6	6.466	431	2.2	0.577811
23	6.563	450	53.8	0.62473	3	3.04989	6.9	6.2	6.563	450.2	2.7	0.634441
24	6.651	460	55	0.167791	2	0.59469	1.3	1.2	6.651	459.8	2.1	0.159514
25	6.722	468	55.9	0.0864992	6	0.873361	2	1.8	6.722	467.4	5.5	0.0892001
26	6.819	479	57.2	0.198068	3	1.10571	2.5	2.3	6.819	479.4	3.1	0.200458
27	6.916	491	58.7	0.0257291	3	0.144468	0.3	0.3	6.916	490.5	3.2	0.02563
28	7.002	502	60	0.112004	3	0.531793	1.2	1.1	7.002	501.9	2.6	0.114949
29	7.112	516	61.6	0.576101	3	2.96747	6.7	6	7.112	516.2	2.9	0.582388
30	7.38	551	65.8	0.024414	6	0.216643	0.5	0.4	7.38	550.5	5.6	0.021732
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.537	575	68.7	0.0240005	3	0.143178	0.3	0.3	7.537	575.1	3.4	0.0236255
32	7.727	604	72.2	0.187726	6	2.10499	4.7	4.3	7.727	603.8	6.4	0.184979
33	7.904	631	75.4	0.202866	7	2.40962	5.4	4.9	7.904	629.7	7.2	0.188537
34	8.771	782	93.4	0.199745	18	6.30619	14.2	12.8	8.771	783.8	18	0.197664
35	9.186	811	96.9	0.146253	9	1.16361	2.6	2.4	9.186	807.4	8.8	0.0742914

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	86	10.3	0.0406783	5	0.325317	3.6	2.7	4.55	86.2	4.6	0.0403109
2	5.2	183	21.8	0.0588578	5	0.550821	6.1	4.6	5.2	183.1	5.5	0.0565922
3	5.85	301	35.9	0.0419547	3	0.252632	2.8	2.1	5.85	300.5	3.4	0.0415551
4	6.55	450	53.7	0.123616	6	1.19084	13.2	10	6.55	449.2	5.9	0.11349
5	6.85	484	57.8	0.0183133	5	0.155986	1.7	1.3	6.85	484.7	5.1	0.0173027
6	7.35	547	65.3	0.0651803	4	0.478437	5.3	4	7.35	546.6	4.1	0.0650725
7	8.15	667	79.6	0.135398	7	1.71151	19	14.4	8.15	667.1	7.2	0.133403
8	8.45	728	86.9	0.135784	8	1.95481	21.7	16.5	8.45	728.5	8.3	0.133393
9	8.65	770	91.9	0.0891028	8	1.17803	13.1	9.9	8.65	771.5	8.2	0.0808231
10	9.3	819	97.7	0.0903131	8	1.19275	13.3	10.1	9.3	816.1	8.5	0.0793888

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.023	18	2.1	0.0461054	5	0.357697	1.1	1.1	4.023	18	4.5	0.0448157
2	4.181	41	4.9	0.0301612	6	0.29466	0.9	0.9	4.181	40.5	5.6	0.029948
3	4.427	77	9.2	0.390349	4	3.11907	9.7	9.3	4.427	77.2	4.5	0.391976
4	4.55	95	11.3	0.0328194	3	0.197368	0.6	0.6	4.55	94.6	3.3	0.0333717
5	4.728	121	14.4	0.0137802	5	0.119927	0.4	0.4	4.728	122.2	5	0.0134284
6	5.07	171	20.3	0.0142911	4	0.100692	0.3	0.3	5.07	171.6	4.2	0.0136246
7	5.2	190	22.6	0.0367982	5	0.311785	1	0.9	5.2	190	5.1	0.0346879
8	5.315	210	25	0.228922	4	1.51901	4.7	4.5	5.315	209.5	3.9	0.219035
9	5.453	234	27.8	0.0402655	12	0.805492	2.5	2.4	5.453	234.1	11.9	0.0381452
10	5.551	251	29.8	0.0542373	6	0.53769	1.7	1.6	5.551	251.3	6.1	0.049872
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.712	279	33.2	0.0226941	8	0.287723	0.9	0.9	5.712	279.3	7.5	0.0216239
12	5.85	303	36	0.423101	3	2.53032	7.9	7.6	5.85	303.5	3.4	0.419569
13	5.94	322	38.3	0.189484	5	1.51342	4.7	4.5	5.94	321.6	4.9	0.173294
14	6.058	347	41.3	0.1201	4	0.835084	2.6	2.5	6.058	347.5	3.9	0.119526
15	6.128	362	43	0.153873	4	1.23532	3.8	3.7	6.128	361.8	4.5	0.156531
16	6.204	378	44.9	0.0334854	4	0.220521	0.7	0.7	6.204	378.2	3.6	0.0347272
17	6.228	383	45.5	0.0487401	2	0.140941	0.4	0.4	6.228	383.2	1.8	0.0440292
18	6.275	393	46.7	0.0098325	2	0.0288812	0.1	0.1	6.275	393.2	1.7	0.0096308
19	6.421	424	50.4	0.152197	8	1.99272	6.2	6	6.421	426	7.9	0.142903
20	6.482	437	52	0.459337	2	1.81862	5.7	5.4	6.482	436.8	2.3	0.440416
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.598	457	54.3	0.520109	3	2.62368	8.2	7.8	6.598	457.1	2.8	0.526415
22	6.682	467	55.5	0.12792	2	0.45838	1.4	1.4	6.682	467.3	2.1	0.125047
23	6.749	475	56.5	0.06489	5	0.609345	1.9	1.8	6.749	474.1	4.9	0.069672
24	6.85	487	57.9	0.130599	3	0.719396	2.2	2.2	6.85	486.4	3.1	0.132984
25	6.937	498	59.2	0.0105533	2	0.0534799	0.2	0.2	6.937	496.9	2.3	0.0132403
26	7.009	507	60.3	0.0706596	2	0.284402	0.9	0.9	7.009	507.6	2.1	0.0747574
27	7.128	522	62.1	0.515623	2	2.20208	6.8	6.6	7.128	521.9	2.4	0.509811
28	7.35	550	65.4	0.0513325	7	0.595255	1.9	1.8	7.35	550.1	7.1	0.0471869
29	7.545	579	68.8	0.0198967	18	0.624126	1.9	1.9	7.545	593.8	18	0.0195629
30	7.733	607	72.2	0.122929	9	1.59979	5	4.8	7.733	607.2	8.8	0.102044
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.928	636	75.6	0.17595	6	1.715	5.3	5.1	7.928	636.8	5.6	0.17241
32	8.15	669	79.5	0.0461543	7	0.579125	1.8	1.7	8.15	668.6	7	0.0463939
33	8.45	734	87.3	0.0773526	5	0.718169	2.2	2.1	8.45	733.2	5.4	0.0757181
34	8.65	778	92.5	0.109922	7	1.07057	3.3	3.2	8.65	778.4	7	0.0859373
35	9.141	812	96.6	0.0387455	4	0.294755	0.9	0.9	9.141	812.2	4.4	0.0380154
36	9.3	823	97.9	0.0122872	2	0.0478326	0.1	0.1	9.3	823.7	2	0.0135391

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.053	19	2.3	0.0549082	5	0.420491	0.9	0.8	4.053	18.6	4.6	0.0519627
2	4.216	43	5.1	0.0345273	6	0.326008	0.7	0.6	4.216	41.5	5.8	0.0318606
3	4.319	58	6.9	0.0492848	5	0.439772	0.9	0.9	4.319	57.9	5.1	0.0489779
4	4.455	78	9.3	0.516071	4	4.00791	8.5	7.9	4.455	78.3	4.3	0.521508
5	4.754	122	14.5	0.0208438	8	0.264831	0.6	0.5	4.754	119.9	7.5	0.0198188
6	5.081	170	20.2	0.0234903	4	0.167883	0.4	0.3	5.081	169.9	4	0.0233968
7	5.253	197	23.4	0.0217532	3	0.0925325	0.2	0.2	5.253	196.2	2.5	0.0206349
8	5.326	210	25	0.415003	3	2.14473	4.6	4.2	5.326	210.6	3.1	0.394964
9	5.466	235	27.9	0.0352713	18	0.941727	2	1.9	5.466	232.2	18	0.0295179
10	5.578	255	30.3	0.0959486	6	0.88302	1.9	1.7	5.578	255.3	6.1	0.081432
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.718	280	33.3	0.0395808	5	0.322621	0.7	0.6	5.718	280	4.9	0.0372957
12	5.847	303	36	0.569141	3	3.39454	7.2	6.7	5.847	303.4	3.5	0.554241
13	5.934	321	38.2	0.26956	6	2.26064	4.8	4.5	5.934	320.8	5.5	0.230891
14	6.054	346	41.1	0.181083	7	1.86778	4	3.7	6.054	345.1	6.7	0.156616
15	6.125	361	42.9	0.213269	4	1.62862	3.5	3.2	6.125	360.2	4.3	0.212559
16	6.221	381	45.3	0.0754313	5	0.548497	1.2	1.1	6.221	378.9	5.3	0.0580914
17	6.413	421	50.1	0.203893	7	2.11862	4.5	4.2	6.413	422.7	7.4	0.161258
18	6.48	435	51.7	0.499152	2	1.88437	4	3.7	6.48	434.5	2.1	0.503781
19	6.595	455	54.1	0.550286	3	2.83408	6	5.6	6.595	454.7	2.9	0.559389
20	6.68	465	55.3	0.153039	7	1.42557	3	2.8	6.68	468.2	7.5	0.107672
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.833	483	57.4	0.183538	3	1.07606	2.3	2.1	6.833	483.3	3.3	0.183903
22	6.929	495	58.9	0.0247326	3	0.125504	0.3	0.2	6.929	494.7	2.9	0.024598
23	7.009	505	60	0.102731	2	0.466873	1	0.9	7.009	505.4	2.5	0.105915
24	7.128	520	61.8	0.562041	3	2.53407	5.4	5	7.128	519.7	2.5	0.569632
25	7.397	555	66	0.0399949	7	0.446933	1	0.9	7.397	555.3	7.1	0.0354377
26	7.557	579	68.8	0.0213599	3	0.122185	0.3	0.2	7.557	578.7	3.5	0.0199367
27	7.737	606	72.1	0.202837	8	2.94829	6.3	5.8	7.737	604.1	8.2	0.204033
28	7.903	631	75	0.245025	10	3.93003	8.4	7.8	7.903	629.5	9.9	0.223854
29	8.774	785	93.3	0.197929	18	6.15127	13.1	12.2	8.774	784.2	18	0.192808
30	9.148	812	96.6	0.136866	7	1.13818	2.4	2.2	9.148	809.5	7.3	0.0876403

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	92	10.9	0.0418211	4	0.260409	2.4	1.8	4.55	91.5	3.5	0.0416809
2	5.2	188	22.3	0.0702596	5	0.53276	4.9	3.6	5.2	188.1	4.6	0.0654913
3	5.85	307	36.4	0.0520413	3	0.284062	2.6	1.9	5.85	307	3.2	0.0505398
4	6.55	451	53.4	0.142284	6	1.44894	13.2	9.8	6.55	450.2	6.2	0.132339
5	6.85	486	57.6	0.0292824	5	0.266146	2.4	1.8	6.85	485.2	5.3	0.0283298
6	7.35	549	65	0.0897018	4	0.658638	6	4.5	7.35	549	4.3	0.0855007
7	8.15	670	79.4	0.101612	12	2.09843	19.2	14.2	8.15	670	12.3	0.0966452
8	8.45	729	86.4	0.189611	9	2.77208	25.3	18.8	8.45	727.4	8.6	0.182877
9	8.65	777	92.1	0.154519	4	1.13992	10.4	7.7	8.65	777	4.3	0.15112
10	9.3	826	97.9	0.123828	8	1.49062	13.6	10.1	9.3	823.9	7.5	0.111802

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 131

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.000 5.200 6.800 5.850 3.600 6.550 15.700 6.850 2.500 7.350 4.600 8.150 18.300 8.450 23.500 8.650 9.600 9.300 10.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.073 1.000 4.228 1.100 4.377 1.900 4.479 9.900 4.770 0.700 5.109 0.500 5.279 0.400 5.344 5.400 5.469 0.300 5.600 2.700 5.730 0.600 5.864 7.600 5.951 3.300 6.071 2.900 6.139 3.600 6.201 0.700 6.230 0.700 6.273 0.100 6.374 0.200 6.403 0.800 6.427 1.900 6.466 5.000 6.563 6.900 6.651 1.300 6.722 2.000 6.819 2.500 6.916 0.300 7.002 1.200 7.112 6.700 7.380 0.500 7.537 0.300 7.727 4.700 7.904 5.400 8.771 14.200 9.186 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 35 Return to Lane Navigator
4.550 3.600 5.200 6.100 5.850 2.800 6.550 13.200 6.850 1.700 7.350 5.300 8.150 19.000 8.450 21.700 8.650 13.100 9.300 13.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.023 1.100 4.181 0.900 4.427 9.700 4.550 0.600 4.728 0.400 5.070 0.300 5.200 1.000 5.315 4.700 5.453 2.500 5.551 1.700 5.712 0.900 5.850 7.900 5.940 4.700 6.058 2.600 6.128 3.800 6.204 0.700 6.228 0.400 6.275 0.100 6.421 6.200 6.482 5.700 6.598 8.200 6.682 1.400 6.749 1.900 6.850 2.200 6.937 0.200 7.009 0.900 7.128 6.800 7.350 1.900 7.545 1.900 7.733 5.000 7.928 5.300 8.150 1.800 8.450 2.200 8.650 3.300 9.141 0.900 9.300 0.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 36 Return to Lane Navigator
4.053 0.900 4.216 0.700 4.319 0.900 4.455 8.500 4.754 0.600 5.081 0.400 5.253 0.200 5.326 4.600 5.466 2.000 5.578 1.900 5.718 0.700 5.847 7.200 5.934 4.800 6.054 4.000 6.125 3.500 6.221 1.200 6.413 4.500 6.480 4.000 6.595 6.000 6.680 3.000 6.833 2.300 6.929 0.300 7.009 1.000 7.128 5.400 7.397 1.000 7.557 0.300 7.737 6.300 7.903 8.400 8.774 13.100 9.148 2.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 30 Return to Lane Navigator
4.550 2.400 5.200 4.900 5.850 2.600 6.550 13.200 6.850 2.400 7.350 6.000 8.150 19.200 8.450 25.300 8.650 10.400 9.300 13.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Drum ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Drum ---- Go to Display Gel Lane Navigator.