RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ruvettus(*) pretiosus(*)
FDA Seafood List Common Name: Oilfish(*)
FDA Seafood List Market Name: Oilfish(*)
Unique IEF Gel Code: 19950831gel14
Unique Web Code: oih03Srr
Tissue Aliquot Code: ruvepret H95-004 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	105	12.1	0.0446627	5	0.336226	11.8	6.7	4.55	104.9	4.8	0.0397232
2	5.2	210	24.3	0.0322182	6	0.333228	11.7	6.7	5.2	209.7	6.4	0.0294621
3	5.85	314	36.3	0.0298032	4	0.201015	7	4	5.85	313.8	4.1	0.027966
4	6.55	431	49.8	0.0252261	8	0.318615	11.2	6.4	6.55	429.7	7.7	0.0231954
5	7.35	522	60.3	0.0121378	4	0.0875474	3.1	1.8	7.35	522.8	4	0.0124294
6	8.15	643	74.3	0.0363532	8	0.500351	17.5	10	8.15	642.3	8.4	0.0337076
7	8.45	701	81	0.0546149	6	0.566738	19.8	11.3	8.45	700.4	6.3	0.0509552
8	8.65	757	87.5	0.0428482	4	0.310327	10.9	6.2	8.65	756.8	4.4	0.03948
9	9.3	816	94.3	0.018157	7	0.203399	7.1	4.1	9.3	816.2	6.7	0.0170832

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.999	17	2	0.0238091	6	0.229615	1.7	1.3	3.999	17.1	5.6	0.0232435
2	4.435	85	9.8	0.195659	4	1.51085	11.3	8.8	4.435	85.4	4.3	0.198831
3	5.634	275	31.8	0.0278205	7	0.299348	2.2	1.7	5.634	276.2	6.8	0.0248165
4	5.807	303	35	0.0246902	4	0.159113	1.2	0.9	5.807	303.9	3.7	0.0243279
5	5.88	315	36.4	0.0117444	3	0.0756195	0.6	0.4	5.88	313.5	3.2	0.0131629
6	5.946	326	37.7	0.349742	3	1.6285	12.2	9.5	5.946	325.8	2.7	0.343303
7	6.012	337	39	0.154879	5	1.27172	9.6	7.4	6.012	337	5.4	0.131655
8	6.102	352	40.7	0.105868	5	0.761495	5.7	4.4	6.102	351.6	4.5	0.0946656
9	6.15	360	41.6	0.107056	4	0.632209	4.7	3.7	6.15	360.7	3.7	0.0971991
10	6.301	385	44.5	0.0352987	3	0.19653	1.5	1.1	6.301	384.8	3.5	0.0317431
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.391	400	46.2	0.0253932	5	0.185692	1.4	1.1	6.391	398.5	5.2	0.0202718
12	6.581	430	49.7	0.102878	7	1.12315	8.4	6.6	6.581	432.3	6.6	0.0961785
13	6.668	440	50.9	0.265863	3	1.03241	7.8	6	6.668	440.4	2.6	0.225231
14	6.764	451	52.1	0.407913	3	2.06673	15.5	12.1	6.764	451.2	2.9	0.407921
15	6.895	466	53.9	0.242122	3	1.17426	8.8	6.9	6.895	466	2.8	0.23607
16	7.782	583	67.4	0.112396	5	0.967999	7.3	5.6	7.782	582.1	5.2	0.105255

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	101	11.7	0.0285896	5	0.232425	10.1	6.6	4.55	101.5	5	0.026295
2	5.2	199	23	0.0187128	8	0.238978	10.4	6.7	5.2	200	8.1	0.0166895
3	5.85	306	35.4	0.015724	5	0.141132	6.1	4	5.85	306.9	5	0.0158498
4	6.55	422	48.8	0.023037	7	0.240405	10.5	6.8	6.55	421.3	6.6	0.0205127
5	7.35	514	59.5	0.009641	5	0.0811808	3.5	2.3	7.35	514.5	5.4	0.0085432
6	8.15	634	73.4	0.0343515	6	0.351714	15.3	9.9	8.15	633.3	6.2	0.0320678
7	8.45	693	80.2	0.0454234	7	0.510499	22.2	14.4	8.45	693.2	6.6	0.0435414
8	8.65	754	87.3	0.0405002	4	0.290761	12.7	8.2	8.65	752.9	4.4	0.0375574
9	9.3	813	94.1	0.0207202	7	0.211149	9.2	6	9.3	812.9	6.7	0.0177408

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.967	23	2.7	0.0094	7	0.086813	1.2	1	3.967	21.4	6.6	0.0073896
2	4.429	92	10.6	0.0770758	4	0.559651	8	6.2	4.429	92.4	4.1	0.0773282
3	4.55	110	12.7	0.0224082	4	0.157964	2.2	1.7	4.55	110	4.1	0.021611
4	5.2	207	24	0.0169553	6	0.151154	2.1	1.7	5.2	206.6	5.6	0.0151606
5	5.85	312	36.1	0.0128389	5	0.0976163	1.4	1.1	5.85	311.7	4.6	0.0120858
6	5.942	326	37.7	0.124018	3	0.593515	8.4	6.6	5.942	326.4	2.8	0.11854
7	6.027	339	39.2	0.0599797	6	0.539103	7.7	6	6.027	338.8	6.1	0.0499903
8	6.131	355	41.1	0.0511072	4	0.343821	4.9	3.8	6.131	354.1	4.1	0.0468001
9	6.184	363	42	0.0468206	5	0.338606	4.8	3.7	6.184	363.3	4.5	0.0422388
10	6.334	386	44.7	0.0124964	2	0.0561672	0.8	0.6	6.334	386.7	2.4	0.0132097
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.445	403	46.6	0.0105899	6	0.0690558	1	0.8	6.445	401.4	5.7	0.0068892
12	6.55	419	48.5	0.0144012	6	0.13877	2	1.5	6.55	419.5	5.7	0.0137282
13	6.657	432	50	0.030254	7	0.42392	6	4.7	6.657	437.3	7.4	0.0325403
14	6.756	444	51.4	0.100696	3	0.382688	5.4	4.2	6.756	443.8	2.6	0.0835525
15	6.847	455	52.7	0.248178	3	1.20894	17.2	13.4	6.847	454.6	2.8	0.244289
16	6.971	470	54.4	0.0989396	3	0.512504	7.3	5.7	6.971	469.4	2.9	0.100062
17	7.35	516	59.7	0.0068191	4	0.0466271	0.7	0.5	7.35	515.9	4	0.0065986
18	7.814	585	67.7	0.0289864	6	0.279119	4	3.1	7.814	583.8	5.8	0.0270557
19	8.15	635	73.5	0.0219319	7	0.265488	3.8	2.9	8.15	634.8	7.3	0.0205326
20	8.45	699	80.9	0.0381224	6	0.370797	5.3	4.1	8.45	699.9	6	0.0347941
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	754	87.3	0.0351106	5	0.287422	4.1	3.2	8.65	753.7	5.3	0.0307467
22	9.3	816	94.4	0.014125	5	0.123243	1.8	1.4	9.3	814.2	5.3	0.0130243

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.904	18	2.1	0.0256683	5	0.221025	2	1.5	3.904	18.5	4.9	0.0255962
2	4.409	90	10.4	0.17292	4	1.37393	12.2	9.3	4.409	89.9	4.5	0.173792
3	4.719	134	15.5	0.0092668	5	0.0816952	0.7	0.6	4.719	136	5.5	0.0084481
4	5.632	274	31.7	0.0250985	6	0.263048	2.3	1.8	5.632	275.2	6.4	0.0231791
5	5.771	297	34.4	0.0120304	3	0.0596583	0.5	0.4	5.771	297.4	2.8	0.0121064
6	5.832	307	35.5	0.0047448	2	0.0135358	0.1	0.1	5.832	306.3	1.9	0.0040905
7	5.895	317	36.7	0.265643	3	1.37134	12.2	9.2	5.895	317.6	2.8	0.272187
8	5.971	329	38.1	0.110683	5	0.894344	8	6	5.971	329	5.4	0.0933451
9	6.08	346	40	0.0703895	4	0.47813	4.3	3.2	6.08	345.7	4.3	0.0632007
10	6.138	355	41.1	0.0637621	3	0.346492	3.1	2.3	6.138	355.5	3.1	0.063412
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.259	374	43.3	0.0073082	2	0.0233106	0.2	0.2	6.259	373.1	1.9	0.0070012
12	6.31	382	44.2	0.0280973	3	0.130568	1.2	0.9	6.31	382.5	2.6	0.0287792
13	6.419	399	46.2	0.0209252	4	0.120089	1.1	0.8	6.419	398.4	3.6	0.0187221
14	6.62	428	49.5	0.0756313	6	0.82119	7.3	5.5	6.62	430.8	6.1	0.0763389
15	6.712	439	50.8	0.225588	3	1.021	9.1	6.9	6.712	439.3	3	0.19081
16	6.811	451	52.2	0.370542	3	1.83893	16.3	12.4	6.811	450.4	2.7	0.379082
17	6.935	466	53.9	0.233411	3	1.13156	10.1	7.6	6.935	465.8	2.8	0.227285
18	7.795	583	67.5	0.116554	5	1.05809	9.4	7.1	7.795	581.8	5.3	0.112006

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	110	12.7	0.036218	5	0.27912	9.5	6.6	4.55	109.4	4.5	0.0348891
2	5.2	198	22.9	0.0258562	4	0.184246	6.3	4.4	5.2	198.9	4.2	0.0245587
3	5.85	308	35.6	0.0207796	4	0.132412	4.5	3.1	5.85	307.9	3.6	0.0208909
4	6.55	420	48.6	0.0198861	12	0.482673	16.4	11.5	6.55	413.4	12	0.0227124
5	7.35	516	59.7	0.0149125	6	0.132976	4.5	3.2	7.35	516.4	6.1	0.0123851
6	8.15	638	73.8	0.0339553	7	0.427605	14.6	10.1	8.15	636.9	7.2	0.0333027
7	8.45	694	80.3	0.0415668	9	0.658297	22.4	15.6	8.45	695.1	9.2	0.0401678
8	8.65	756	87.5	0.056844	5	0.443486	15.1	10.5	8.65	755.2	4.6	0.0539455
9	9.3	816	94.4	0.0219532	5	0.194214	6.6	4.6	9.3	815.2	5.5	0.02

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 83

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.800 5.200 11.700 5.850 7.000 6.550 11.200 7.350 3.100 8.150 17.500 8.450 19.800 8.650 10.900 9.300 7.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
3.999 1.700 4.435 11.300 5.634 2.200 5.807 1.200 5.880 0.600 5.946 12.200 6.012 9.600 6.102 5.700 6.150 4.700 6.301 1.500 6.391 1.400 6.581 8.400 6.668 7.800 6.764 15.500 6.895 8.800 7.782 7.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 10.100 5.200 10.400 5.850 6.100 6.550 10.500 7.350 3.500 8.150 15.300 8.450 22.200 8.650 12.700 9.300 9.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
3.967 1.200 4.429 8.000 4.550 2.200 5.200 2.100 5.850 1.400 5.942 8.400 6.027 7.700 6.131 4.900 6.184 4.800 6.334 0.800 6.445 1.000 6.550 2.000 6.657 6.000 6.756 5.400 6.847 17.200 6.971 7.300 7.350 0.700 7.814 4.000 8.150 3.800 8.450 5.300 8.650 4.100 9.300 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
3.904 2.000 4.409 12.200 4.719 0.700 5.632 2.300 5.771 0.500 5.832 0.100 5.895 12.200 5.971 8.000 6.080 4.300 6.138 3.100 6.259 0.200 6.310 1.200 6.419 1.100 6.620 7.300 6.712 9.100 6.811 16.300 6.935 10.100 7.795 9.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 9.500 5.200 6.300 5.850 4.500 6.550 16.400 7.350 4.500 8.150 14.600 8.450 22.400 8.650 15.100 9.300 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Oilfish(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.