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Quantitative immunofluorescent measurements of surface receptors on immune subsets from HIV infected children. American Pediatric Society 104th annual meeting and Society for Pediatric Research 63rd annual meeting; 1994 May 2-5; Seattle.

Denny TN, Stein D, Ince L, Mui T, Dieudonne A, Oleske JM.

Pediatr AIDS HIV Infect. 1994 Oct; 5: 317-24.

Department of Pediatrics, New Jersey Medical School, Newark, USA.

It is possible to determine the antibody binding Capacity (ABC) per cell by performing quantitative immunofluorescent flow cytometric analysis of immune subsets. This study evaluated the number of ABC for CD3, CD4, CD8, CD16, and CD19 on lymphocytes and for CD4 and HLA-DR on monocytes. Peripheral blood samples were available from 16 HIV children that were grouped and staged as follows: Group I-asymptomatic (mean age = 1.7 yrs/n = 3); Group II-symptomatic (mean age = 2.4 yrs/n = 6); Group III-AIDS (mean age = 4.0 yrs/n = 3) and Group IV-long term survivors (mean age = 10.5 yrs/n = 4). Data is expressed as mean number +/- SD of ABC/cell for each disease group. ABC for lymphocyte markers were as follows: CD3 (I = 120,050 +/- 1586, II = 122,465 +/- 13864, III = 101,845 +/- 2818, IV = 95,348 +/- 11018); CD4 (I = 50,302 +/- 4284, II = 54,157 +/- 4323, III = 50,949 +/- 5166, IV = 55,124 +/- 5039); CD8 (I = 192,205 +/- 4477, II = 200,395 +/- 22683, III = 200,820 +/- 34018, IV = 182,990 +/- 20864); CD16 (I = 17,536 +/- 7504, II = 13,940 +/- 10362, III = 12,025 +/- 5908, IV = 10,988 +/- 3730) and CD19 (I = 28,105 +/- 1814, II = 29,085 +/- 4516, III = 26,898 +/- 11407, IV = 23,693 +/- 2950) Monocyte expression of CD4 yielded (I = 9,684 +/- 1146, II = 11,991 +/- 2491, III = 11,030 +/- 1540, IV = 11212 +/- 1937) and HLA-DR was (I = 105,311 +/- 16316, II = 133,886 +/- 9265, III = 144,909 +/- 22419, IV = not done). These studies suggest a down regulation of CD3, CD16 and CD19 on lymphocytes and an up regulation of CD4 and HLA-DR on monocytes with HIV disease progression. Studies are underway to evaluate the potential influence of age on the distribution of ABC/cell and correlation with disease progression.

Publication Types:
  • Meeting Abstracts
Keywords:
  • AIDS Vaccines
  • Acquired Immunodeficiency Syndrome
  • Anti-HIV Agents
  • Antigens, CD19
  • Antigens, CD3
  • Antigens, CD4
  • Antigens, CD8
  • Child
  • FCGR3B protein, human
  • HIV
  • HIV Infections
  • HIV Seropositivity
  • HLA-DR Antigens
  • Humans
  • Receptors, IgG
  • immunology
  • methods
  • organization & administration
Other ID:
  • 95330440
UI: 102212495

From Meeting Abstracts




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