Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19970424gel02
Unique Web Code: rsh02Srr
Tissue Aliquot Code: lutjcamp D97-002 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559511.10.064283630.3752856.54.64.5595.43.40.0628609
25.219122.30.062896340.4578885.65.2191.14.30.059394
35.8530335.40.032307430.1850353.22.35.85302.93.20.0322407
46.5544852.40.12795351.0011117.412.26.55447.34.70.118985
56.8548456.60.021039950.1556082.71.96.85484.34.60.0192131
67.3555364.70.043282940.3167625.53.97.35552.14.30.0418416
78.1567578.90.062517980.90199315.7118.15673.48.20.0621282
88.4574186.70.09878361.118319.413.68.45741.46.40.0990285
98.6579192.50.092213740.60655310.57.48.65790.43.80.0899505
109.383597.70.078053550.632398117.79.3833.550.0715121
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.104313.60.041456750.313240.80.74.10431.14.60.0387088
24.308617.10.36167853.314138.47.34.30861.15.20.356516
34.86614316.70.015899840.1171240.30.34.866143.94.50.0148278
45.03716819.60.1582551.310193.32.95.037169.15.20.143319
55.275205240.023146940.1637660.40.45.2752044.30.0215846
65.36722125.80.45240831.9505554.35.367221.32.70.400857
75.49324328.40.0516853182.112145.44.75.493233.6180.0662039
85.59126030.40.085641991.121122.82.55.591261.58.60.073866
95.67727532.20.12050651.055692.72.35.677274.75.20.113633
105.87431036.30.014475530.07391480.20.25.874309.63.10.0136431
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.98533338.90.51955432.853617.26.35.985332.73.10.520082
126.07635241.20.25430541.745474.43.86.076352.34.20.233922
136.192376440.20810162.144715.44.76.1923746.30.192477
146.273393460.017736650.1335590.30.36.273395.14.60.0164003
156.3641148.10.06393620.2599130.70.66.36410.92.30.0638307
166.38941748.80.026702920.0892040.20.26.389416.71.90.0264328
176.48543751.10.035635860.3654970.90.86.485438.55.90.034737
186.524445520.22374230.9770732.52.26.524445.22.60.21508
196.65746454.30.60437133.694239.48.16.657464.33.50.597579
206.73647455.40.4962242.799977.16.26.736474.23.60.440578
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.84648857.10.24514583.051787.86.76.846487.27.60.225231
227.2754463.60.19138641.406233.63.17.27544.34.40.179372
237.68860570.80.086458962.534186.45.67.688620.65.50.258787
247.91663974.70.195003185.7901714.712.87.916627.6180.18149
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559611.30.023402240.1777214.12.94.5596.44.40.0229161
25.219122.40.028638370.3534598.15.75.2189.47.50.0266076
35.8530535.80.024553440.1623513.72.65.85305.540.0231815
46.5545152.90.050049470.57789313.39.46.55449.77.40.0442004
56.8549157.60.008086450.05817611.30.96.85490.94.50.0072898
67.3555464.90.028656940.2002664.63.27.35553.240.0279837
78.1567178.70.063906270.82899719.113.48.15670.47.40.0635836
88.4574387.10.10237961.0142623.316.48.45741.95.70.10104
98.6578792.30.062164440.46358110.77.58.65786.14.30.0603204
109.382997.20.053097260.50793211.78.29.3827.960.047628
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.137313.60.029638440.1860060.80.64.13730.93.60.029209
24.32586.80.25759352.271969.87.74.3258.45.10.250687
34.559210.80.026463330.1653950.70.64.5592.33.50.0269275
44.87514016.50.009470840.06881840.30.24.875138.44.30.0089445
55.03116319.20.15946651.400546.14.85.031162.55.30.148919
65.218822.10.0333976160.793423.42.75.2185.215.80.0283114
75.33921224.90.22953241.535346.65.25.339212.14.20.205556
85.59925730.20.018563480.23769610.85.599258.98.20.0163866
95.69827432.20.032300650.2588891.10.95.6982735.20.0278544
106.03233839.70.37342942.5144910.98.56.032337.83.90.359905
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.11935641.80.19322951.590936.95.46.119355.65.10.17533
126.21537644.20.1626661.723367.55.96.215374.25.80.167625
136.30239446.30.017028640.1305140.60.46.302393.54.50.0164293
146.37941048.20.022762810.07213230.30.26.379409.51.40.0300011
156.53444251.90.15975230.9142643.16.534441.83.40.151755
166.66846154.20.49786732.8312612.39.66.668460.93.20.495558
176.74247155.30.33840931.671997.25.76.742470.53.20.29811
186.847485570.10838491.660917.25.66.847482.19.40.0993454
197.3554263.70.13665930.69745932.47.35542.230.133376
207.99164475.70.083800781.126324.93.87.991643.67.70.0830071
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.4574287.20.049097640.3559331.51.28.45742.24.10.0491682
228.6578291.90.035459840.2659881.20.98.65782.74.20.0356807
239.382596.90.0394927110.6210952.72.19.3822.210.90.0321113
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.149354.10.040798340.2830570.90.74.14935.24.10.0392789
24.345647.50.31518652.833318.87.34.34564.85.20.307942
34.9114817.30.010285660.1000510.30.34.91146.55.90.0095929
45.065171200.22960552.028616.35.25.0651715.20.220737
55.39422426.20.27191462.722978.475.3942245.70.271354
65.64626731.30.024045570.31816910.85.646268.37.50.0240488
75.7428333.10.041778860.420631.31.15.742835.80.040669
86.085350410.40993753.6245611.29.36.085348.95.20.391544
96.17236843.10.20165662.118386.65.46.172367.85.90.201381
106.26538745.30.23228172.801948.77.26.265386.46.90.229496
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.34340347.20.05253260.6117631.91.66.343401.56.10.0561961
126.4241949.10.032864840.220010.70.66.42419.540.0312936
136.56844852.50.15436141.002523.12.66.568447.93.70.15139
146.7146754.70.50718843.9050412.1106.714674.30.510957
156.78547755.90.35771742.2482775.86.785477.13.70.340175
166.90949357.70.13925592.2572975.86.909490.59.40.135559
177.35354864.20.15197151.214613.83.17.353547.84.60.147605
187.98164775.80.119044243.6039611.29.27.981643.1240.0847232
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.5594110.031259570.3442795.244.5592.26.90.0282855
25.219122.40.0232184100.3876885.84.55.2186.8100.0217994
35.8530035.20.02303170.26830743.15.85299.66.90.0219637
46.5544351.90.085203881.1994118146.55442.18.40.0807303
56.8548356.60.017364450.1460332.21.76.854835.50.0150043
67.3555064.50.033653750.26838143.17.35549.54.80.0314355
78.1567579.10.073884981.0860416.312.78.15673.98.40.0730477
88.4574086.80.12364761.3910720.916.38.45739.16.40.121881
98.6578892.40.081942240.6338329.57.48.65787.54.40.0809175
109.383097.30.083316170.94567414.2119.3827.77.50.071477
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 95
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln