NT0210

Library Description

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

130 ESTs from this library were grouped into 108 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM		UniGene Entry
7	53846		Hs.436657	Clusterin.
4	30769		Hs.515070	Eukaryotic translation elongation factor 2.
4	30769		Hs.458355	Complement component 1, s subcomponent.
3	23077		Hs.195464	Filamin A, alpha (actin binding protein 280).
2	15385		Hs.77269	Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2.
2	15385		Hs.694849	Transcribed locus, weakly similar to NP_179188.1 leucine-rich repeat family protein / extensin family protein [Arabidopsis thaliana].
2	15385		Hs.603111	Rho family GTPase 2.
2	15385		Hs.514227	Glial fibrillary acidic protein.
2	15385		Hs.35758	MYST histone acetyltransferase (monocytic leukemia) 4.
2	15385		Hs.188634	Sorting nexin 1.
2	15385		Hs.136905	HECT, UBA and WWE domain containing 1.
2	15385		Hs.125898	GNAS complex locus.
1	7692		Hs.90073	CSE1 chromosome segregation 1-like (yeast).
1	7692		Hs.7367	BTB (POZ) domain containing 6.
1	7692		Hs.716582	Transcribed locus, strongly similar to XP_001516585.1 PREDICTED: similar to predicted protein, partial [Ornithorhynchus anatinus].
1	7692		Hs.716392	RAB7A, member RAS oncogene family.
1	7692		Hs.714326	CDNA FLJ34896 fis, clone NT2NE2018180.
1	7692		Hs.712847	NADPH dependent diflavin oxidoreductase 1.
1	7692		Hs.710679	Transcribed locus.
1	7692		Hs.709297	Protein phosphatase 1, regulatory (inhibitor) subunit 13B.
1	7692		Hs.706355	Integrin-linked kinase.
1	7692		Hs.705490	ER lipid raft associated 2.
1	7692		Hs.695347	CDNA clone IMAGE:40132509.
1	7692		Hs.679046	RAB9B, member RAS oncogene family.
1	7692		Hs.657969	Zinc finger protein 142.
1	7692		Hs.656492	Ankyrin repeat and sterile alpha motif domain containing 1A.
1	7692		Hs.65135	KIAA0913.
1	7692		Hs.647419	CD68 molecule.
1	7692		Hs.643267	RAD23 homolog A (S. cerevisiae).
1	7692		Hs.632443	Hypothetical FLJ39739, mRNA (cDNA clone IMAGE:40146984).
1	7692		Hs.631992	Dystonin.
1	7692		Hs.631587	TBC1 domain family, member 17.
1	7692		Hs.629152	Transcribed locus.
1	7692		Hs.62886	SPARC-like 1 (hevin).
1	7692		Hs.625725	Basal cell adhesion molecule (Lutheran blood group).
1	7692		Hs.597664	Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta.
1	7692		Hs.592414	Homo sapiens, clone IMAGE:4214654, mRNA.
1	7692		Hs.585374	Neuron navigator 1.
1	7692		Hs.573780	Transcribed locus, weakly similar to XP_001718401.1 PREDICTED: similar to hCG2012269 [Homo sapiens].
1	7692		Hs.572535	Leukocyte-associated immunoglobulin-like receptor 1.
1	7692		Hs.567968	Transcribed locus.
1	7692		Hs.567438	Brain protein I3.
1	7692		Hs.5662	Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
1	7692		Hs.555970	Small nuclear ribonucleoprotein polypeptide N.
1	7692		Hs.534847	Complement component 4A (Rodgers blood group).
1	7692		Hs.530314	Sjogren syndrome nuclear autoantigen 1.
1	7692		Hs.525626	Phosphofurin acidic cluster sorting protein 2.
1	7692		Hs.525600	Heat shock protein 90kDa alpha (cytosolic), class A member 1.
1	7692		Hs.522507	F-box and WD repeat domain containing 5.
1	7692		Hs.522472	Bromodomain containing 3.
1	7692		Hs.522019	ADAMTS-like 1.
1	7692		Hs.518460	Adaptor-related protein complex 2, mu 1 subunit.
1	7692		Hs.517357	DiGeorge syndrome critical region gene 2.
1	7692		Hs.515154	F-box and WD repeat domain containing 9.
1	7692		Hs.506759	ATPase, Ca++ transporting, cardiac muscle, slow twitch 2.
1	7692		Hs.504062	Pleckstrin homology-like domain, family B, member 1.
1	7692		Hs.503178	Spectrin, beta, non-erythrocytic 1.
1	7692		Hs.502917	Copper chaperone for superoxide dismutase.
1	7692		Hs.502775	Phospholipase A2, group XVI.
1	7692		Hs.497788	Glutamyl-prolyl-tRNA synthetase.
1	7692		Hs.490551	Ubiquitin associated protein 2-like.
1	7692		Hs.487774	Heterogeneous nuclear ribonucleoprotein A2/B1.
1	7692		Hs.476018	Catenin (cadherin-associated protein), beta 1, 88kDa.
1	7692		Hs.471851	High density lipoprotein binding protein.
1	7692		Hs.466766	Latent transforming growth factor beta binding protein 4.
1	7692		Hs.466015	Zinc finger, SWIM-type containing 4.
1	7692		Hs.465337	PH domain and leucine rich repeat protein phosphatase.
1	7692		Hs.462323	Nuclear receptor co-repressor 1.
1	7692		Hs.444767	Kinesin family member 13B.
1	7692		Hs.440968	Tumor protein p53 binding protein 1.
1	7692		Hs.440379	Rho GTPase-activating protein.
1	7692		Hs.436996	Phosphatase and actin regulator 1.
1	7692		Hs.428446	Carbonic anhydrase XI.
1	7692		Hs.420830	Hypoxia inducible factor 3, alpha subunit.
1	7692		Hs.416024	Neurensin 2.
1	7692		Hs.413133	Fizzy/cell division cycle 20 related 1 (Drosophila).
1	7692		Hs.388827	Regulatory factor X, 4 (influences HLA class II expression).
1	7692		Hs.38621	Sorbin and SH3 domain containing 1.
1	7692		Hs.373793	Rho GTPase activating protein 17.
1	7692		Hs.371021	Lysosomal multispanning membrane protein 5.
1	7692		Hs.363308	ORAI calcium release-activated calcium modulator 2.
1	7692		Hs.36053	Chromosome 5 open reading frame 13.
1	7692		Hs.356604	WNK lysine deficient protein kinase 1.
1	7692		Hs.335003	Ankyrin repeat domain 11.
1	7692		Hs.300224	Chromosome 8 open reading frame 30A.
1	7692		Hs.276315	Family with sequence similarity 118, member B.
1	7692		Hs.273621	2',3'-cyclic nucleotide 3' phosphodiesterase.
1	7692		Hs.27182	Phospholipase A2-activating protein.
1	7692		Hs.222510	DAZ associated protein 1.
1	7692		Hs.21611	Kinesin family member 3C.
1	7692		Hs.203717	Fibronectin 1.
1	7692		Hs.199850	Dachsous 1 (Drosophila).
1	7692		Hs.199343	Protocadherin alpha cluster.
1	7692		Hs.181244	Major histocompatibility complex, class I, A.
1	7692		Hs.179986	Flotillin 1.
1	7692		Hs.170355	Mesenchyme homeobox 2.
1	7692		Hs.169611	Diablo homolog (Drosophila).
1	7692		Hs.166924	SEC13 homolog (S. cerevisiae).
1	7692		Hs.156727	Ankylosis, progressive homolog (mouse).
1	7692		Hs.155247	Aldolase C, fructose-bisphosphate.

Source: NCBI UniGene

EST Sequences

226 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

• brain
• adult

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).