RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Gray Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Glyptocephalus cynoglossus
FDA Seafood List Common Name: Gray Sole
FDA Seafood List Market Name: Sole (Flounder)
Unique IEF Gel Code: 19980331gel06
Unique Web Code: wth01Srr
Tissue Aliquot Code: glypcyno H94-003 #07m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	60	7.4	0.105277	4	0.655293	8.7	6.1	4.55	60.3	3.7	0.0998585
2	5.2	151	18.7	0.0980231	5	0.759722	10.1	7.1	5.2	151.5	4.5	0.094642
3	5.85	252	31.3	0.0584485	5	0.491679	6.5	4.6	5.85	251.3	5	0.0549532
4	6.55	396	49.1	0.149006	7	1.70983	22.7	16	6.55	395.5	7	0.137892
5	6.85	430	53.3	0.0501104	4	0.332735	4.4	3.1	6.85	430.4	4.1	0.04613
6	7.35	503	62.4	0.069379	4	0.471631	6.3	4.4	7.35	503.2	3.9	0.0680785
7	8.15	625	77.5	0.100269	6	1.10784	14.7	10.4	8.15	625.2	6.4	0.0980711
8	8.45	688	85.4	0.0880446	8	1.18396	15.7	11.1	8.45	687.3	7.9	0.084486
9	8.65	738	91.6	0.093912	5	0.822355	10.9	7.7	8.65	737.4	5.1	0.0913163

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.206	11	1.4	0.0190879	4	0.116489	0.5	0.4	4.206	10.8	3.8	0.0173989
2	4.394	38	4.7	0.0615839	5	0.568447	2.6	1.9	4.394	39.1	5.1	0.0632103
3	4.574	64	7.9	0.277344	9	2.63706	12.3	9	4.574	67.2	8.6	0.173753
4	4.978	122	15.1	0.015928	18	3.19114	14.9	10.8	4.978	71.4	18	0.100025
5	5.179	151	18.6	0.0308385	2	0.103991	0.5	0.4	5.179	150.4	1.8	0.0332726
6	5.246	161	19.9	0.078925	2	0.266265	1.2	0.9	5.246	160.7	1.9	0.0799591
7	5.319	172	21.2	0.0599076	2	0.220522	1	0.7	5.319	171.5	2	0.0626269
8	5.365	179	22.1	0.39265	2	1.64619	7.7	5.6	5.365	179	2.4	0.390751
9	5.411	186	23	0.418805	2	1.88323	8.8	6.4	5.411	186.4	2.5	0.426683
10	5.629	219	27	0.441219	3	2.51329	11.7	8.5	5.629	219.6	3.4	0.422583
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.715	232	28.6	0.141648	9	2.09471	9.8	7.1	5.715	231.6	8.8	0.133824
12	5.814	247	30.5	0.231264	4	1.45449	6.8	4.9	5.814	247.3	3.9	0.21158
13	6.204	324	40	0.243729	7	2.16418	10.1	7.3	6.204	324.5	6.9	0.178215
14	6.382	360	44.4	0.11596	17	2.6169	12.2	8.9	6.382	357.1	17.1	0.0861484

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	61	7.5	0.0794617	4	0.586181	8	5.5	4.55	61.5	4.3	0.077402
2	5.2	157	19.4	0.0864559	5	0.739819	10.1	7	5.2	156.1	5.2	0.0799855
3	5.85	253	31.2	0.023296	5	0.218555	3	2.1	5.85	251.9	5.2	0.0236163
4	6.55	392	48.3	0.131802	7	1.56473	21.3	14.7	6.55	391.4	7.2	0.121912
5	6.85	427	52.7	0.0467397	5	0.363066	4.9	3.4	6.85	427.6	5.3	0.038849
6	7.35	497	61.3	0.071871	4	0.473557	6.4	4.4	7.35	497	3.9	0.0691643
7	8.15	625	77.1	0.0856664	8	1.20789	16.5	11.3	8.15	624.6	8.4	0.0807184
8	8.45	685	84.5	0.112948	7	1.41171	19.2	13.3	8.45	684.8	7.2	0.110627
9	8.65	736	90.8	0.100655	5	0.776915	10.6	7.3	8.65	734.9	4.6	0.0957047

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.215	11	1.4	0.0116231	5	0.0949544	0.5	0.4	4.215	10.4	4.8	0.011226
2	4.427	42	5.2	0.0283227	5	0.256018	1.3	1	4.427	41.6	5.2	0.0277561
3	4.55	60	7.4	0.0204618	1	0	0	0	4.55	54	1	0
4	4.632	72	8.9	0.239514	12	4.56142	22.8	17.6	4.632	76.4	11.6	0.221423
5	5.2	155	19.1	0.0396111	6	0.446152	2.2	1.7	5.2	154	6.5	0.0388116
6	5.258	164	20.2	0.0287205	2	0.0824495	0.4	0.3	5.258	164	2.3	0.0206043
7	5.31	172	21.2	0.0146367	1	0.00308354	0	0	5.31	171.2	0.6	0.00283617
8	5.388	184	22.7	0.217925	8	3.12384	15.6	12.1	5.388	185.3	8.2	0.214591
9	5.642	223	27.5	0.380491	3	2.18326	10.9	8.4	5.642	223.5	3.4	0.36053
10	5.727	236	29.1	0.115151	9	1.79184	9	6.9	5.727	235.2	9	0.1119
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.824	251	30.9	0.217379	5	1.60655	8	6.2	5.824	251.5	4.8	0.189816
12	6.224	326	40.1	0.201794	6	1.48428	7.4	5.7	6.224	326.8	5.6	0.14978
13	6.429	365	45	0.0843691	6	0.829277	4.2	3.2	6.429	365.2	5.7	0.0820219
14	6.55	388	47.8	0.0159027	5	0.136232	0.7	0.5	6.55	389.1	4.5	0.0169642
15	6.85	427	52.6	0.0141407	5	0.104921	0.5	0.4	6.85	428	4.7	0.0127252
16	7.35	499	61.5	0.0643578	11	1.03238	5.2	4	7.35	497	10.6	0.0548876
17	7.431	512	63.1	0.0416759	6	0.364055	1.8	1.4	7.431	515.1	5.6	0.0365653
18	8.15	628	77.3	0.0265785	23	1.07531	5.4	4.2	8.15	615.2	22.6	0.0268548
19	8.45	695	85.6	0.0376002	7	0.469801	2.4	1.8	8.45	693.4	7.1	0.03721
20	8.65	740	91.1	0.0414861	5	0.32405	1.6	1.3	8.65	739.1	4.6	0.0397101

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.391	33	4.1	0.0352155	8	0.463856	2.7	1.7	4.391	33.6	8	0.03287
2	4.605	64	7.9	0.2751	10	4.77031	27.5	17.2	4.605	66.8	10.1	0.267114
3	5.193	149	18.3	0.0181532	3	0.0985872	0.6	0.4	5.193	148.8	2.8	0.0196689
4	5.262	160	19.7	0.0523677	3	0.268961	1.6	1	5.262	159.3	2.8	0.0548328
5	5.33	171	21	0.0365914	6	0.212999	1.2	0.8	5.33	177.2	5.6	0.0212773
6	5.417	185	22.8	0.327365	8	3.62206	20.9	13	5.417	182.5	7.6	0.270382
7	5.621	218	26.8	0.292466	4	1.80482	10.4	6.5	5.621	217.9	3.8	0.265481
8	5.695	230	28.3	0.0661879	11	1.38087	8	5	5.695	227.7	11.2	0.0694635
9	5.794	246	30.3	0.2037	5	1.60989	9.3	5.8	5.794	246	4.8	0.189
10	6.208	325	40	0.228206	6	1.94034	11.2	7	6.208	324.8	6.1	0.180133
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.392	361	44.4	0.107518	6	1.15882	6.7	4.2	6.392	361.9	6.4	0.102878

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	55	6.7	0.128809	4	0.774985	9.2	6.4	4.55	55.7	3.5	0.1249
2	5.2	148	18.1	0.12689	4	0.856775	10.1	7.1	5.2	148	4.1	0.118465
3	5.85	258	31.6	0.0615135	5	0.483203	5.7	4	5.85	257.6	4.6	0.0589263
4	6.55	399	48.9	0.147833	6	1.46651	17.4	12.2	6.55	398.7	6.3	0.130679
5	6.85	435	53.3	0.0431568	5	0.356549	4.2	3	6.85	435.4	5.1	0.0394774
6	7.35	504	61.8	0.0666882	4	0.485882	5.8	4	7.35	503.6	4.1	0.0670723
7	8.15	633	77.6	0.1138	8	1.58917	18.8	13.2	8.15	632.5	8.2	0.10934
8	8.45	697	85.4	0.0919994	9	1.52082	18	12.6	8.45	696.3	9.3	0.0924691
9	8.65	743	91.1	0.10717	5	0.9107	10.8	7.6	8.65	743.3	5	0.10307

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 72

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 8.700 5.200 10.100 5.850 6.500 6.550 22.700 6.850 4.400 7.350 6.300 8.150 14.700 8.450 15.700 8.650 10.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.206 0.500 4.394 2.600 4.574 12.300 4.978 14.900 5.179 0.500 5.246 1.200 5.319 1.000 5.365 7.700 5.411 8.800 5.629 11.700 5.715 9.800 5.814 6.800 6.204 10.100 6.382 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 8.000 5.200 10.100 5.850 3.000 6.550 21.300 6.850 4.900 7.350 6.400 8.150 16.500 8.450 19.200 8.650 10.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.215 0.500 4.427 1.300 4.550 0 4.632 22.800 5.200 2.200 5.258 0.400 5.310 0 5.388 15.600 5.642 10.900 5.727 9.000 5.824 8.000 6.224 7.400 6.429 4.200 6.550 0.700 6.850 0.500 7.350 5.200 7.431 1.800 8.150 5.400 8.450 2.400 8.650 1.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 20 Return to Lane Navigator
4.391 2.700 4.605 27.500 5.193 0.600 5.262 1.600 5.330 1.200 5.417 20.900 5.621 10.400 5.695 8.000 5.794 9.300 6.208 11.200 6.392 6.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator
4.550 9.200 5.200 10.100 5.850 5.700 6.550 17.400 6.850 4.200 7.350 5.800 8.150 18.800 8.450 18.000 8.650 10.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Gray Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Gray Sole ---- Go to Display Gel Lane Navigator.