RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Rex Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Errex zachirus
FDA Seafood List Common Name: Rex Sole
FDA Seafood List Market Name: Sole (Flounder)
Unique IEF Gel Code: 19971126gel05
Unique Web Code: rxh02Srr
Tissue Aliquot Code: errezach I94-003 #02m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	33	4.1	0.129251	5	0.94369	11.2	8.4	4.55	33.6	4.5	0.117698
2	5.2	122	15.2	0.104572	4	0.733971	8.7	6.5	5.2	122.5	4.2	0.0981974
3	5.85	228	28.5	0.158499	3	0.945799	11.2	8.4	5.85	228.3	3.4	0.15881
4	6.55	380	47.5	0.138673	7	1.54935	18.3	13.7	6.55	378.6	6.6	0.132948
5	6.85	413	51.6	0.0269635	5	0.220594	2.6	2	6.85	413.6	5.3	0.0236124
6	7.35	487	60.9	0.0864998	5	0.624115	7.4	5.5	7.35	486.7	4.5	0.0781689
7	8.15	617	77.1	0.0997924	7	1.12007	13.2	9.9	8.15	615.7	6.6	0.0953674
8	8.45	684	85.5	0.103519	6	1.16382	13.8	10.3	8.45	683.7	6.4	0.102108
9	8.65	734	91.8	0.100005	4	0.651073	7.7	5.8	8.65	733.6	3.9	0.0934547
10	9.3	778	97.2	0.0645724	5	0.510363	6	4.5	9.3	777.3	4.8	0.0594953

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.361	6	0.8	0.313125	4	2.01268	11.9	9.5	4.361	6.8	3.6	0.315641
2	4.695	51	6.4	0.120716	17	3.63146	21.5	17.1	4.695	55.6	16.7	0.122915
3	5.336	141	17.7	0.387887	2	1.66771	9.9	7.8	5.336	141	2.4	0.390448
4	5.379	148	18.5	0.367926	3	1.90314	11.3	8.9	5.379	148.3	3	0.360426
5	5.52	171	21.4	0.19293	3	0.996625	5.9	4.7	5.52	170.8	3.3	0.171742
6	5.6	184	23.1	0.0301349	10	0.757375	4.5	3.6	5.6	179.1	10	0.0426103
7	5.687	198	24.8	0.0449913	2	0.139198	0.8	0.7	5.687	198.1	2.1	0.037915
8	5.798	216	27.1	0.173948	2	0.647881	3.8	3	5.798	215.8	2.1	0.174105
9	6.223	306	38.3	0.212031	8	2.33135	13.8	11	6.223	306.7	8.3	0.157557
10	6.287	320	40.1	0.147394	9	2.13623	12.7	10	6.287	322.5	8.7	0.138107
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.452	356	44.6	0.0305642	10	0.5286	3.1	2.5	6.452	356.7	10.2	0.0293676
12	6.52	371	46.5	0.0258553	5	0.129988	0.8	0.6	6.52	372.8	4.7	0.0155418

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	30	3.8	0.108449	5	0.826271	10.5	8	4.55	31.2	4.7	0.0983524
2	5.2	116	14.6	0.0791948	5	0.755546	9.6	7.3	5.2	115.2	5.4	0.0785109
3	5.85	221	27.8	0.140547	4	0.963196	12.3	9.3	5.85	220.5	3.8	0.141274
4	6.55	375	47.1	0.126183	7	1.3749	17.5	13.3	6.55	373.6	6.7	0.115046
5	6.85	407	51.1	0.0315352	5	0.256469	3.3	2.5	6.85	408.2	5.1	0.0282743
6	7.35	481	60.4	0.0762744	4	0.50526	6.4	4.9	7.35	481	4	0.0716117
7	8.15	611	76.8	0.0921407	7	1.03207	13.1	10	8.15	611.1	6.5	0.089554
8	8.45	680	85.4	0.0995735	6	1.02675	13.1	10	8.45	679.6	5.9	0.0980227
9	8.65	731	91.8	0.0902941	4	0.601018	7.7	5.8	8.65	730.1	3.9	0.0867237
10	9.3	776	97.5	0.0525579	7	0.508995	6.5	4.9	9.3	774.6	6.6	0.0435074

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.358	15	1.9	0.333612	4	2.39333	10.3	9.1	4.358	15.6	4.3	0.313644
2	4.55	38	4.8	0.0943486	12	1.20868	5.2	4.6	4.55	27.8	12.2	0.055681
3	4.708	57	7.2	0.154597	19	5.14258	22.1	19.4	4.708	59.6	18.8	0.154031
4	5.2	116	14.6	0.032635	4	0.255103	1.1	1	5.2	116.9	4.5	0.0322041
5	5.358	142	17.9	0.180874	4	0.68262	2.9	2.6	5.358	140.5	4.4	0.0881049
6	5.382	146	18.4	0.281535	6	2.67899	11.5	10.1	5.382	147.1	6.5	0.233438
7	5.534	171	21.5	0.138479	5	1.05909	4.6	4	5.534	170.8	5.1	0.116736
8	5.595	181	22.8	0.0654091	10	1.24687	5.4	4.7	5.595	180.1	9.9	0.0710917
9	5.698	198	24.9	0.051439	8	0.630981	2.7	2.4	5.698	196.9	7.5	0.0473148
10	5.807	216	27.2	0.0953312	4	0.611117	2.6	2.3	5.807	215.9	3.7	0.0927289
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	223	28.1	0.0554382	3	0.324369	1.4	1.2	5.85	223.3	3.4	0.053324
12	6.244	308	38.7	0.138313	6	1.19718	5.2	4.5	6.244	307.8	6	0.113136
13	6.323	325	40.9	0.113573	11	2.11081	9.1	8	6.323	324.5	10.7	0.1111
14	6.494	362	45.5	0.0753954	5	0.559157	2.4	2.1	6.494	361.8	5.1	0.0621001
15	6.55	374	47	0.0558265	8	0.776294	3.3	2.9	6.55	373.4	7.7	0.0567389
16	6.85	413	51.9	0.0114388	6	0.117303	0.5	0.4	6.85	413.4	6.3	0.0105124
17	7.35	484	60.9	0.0203657	4	0.153009	0.7	0.6	7.35	484.7	4.4	0.019834
18	8.15	610	76.7	0.0332542	19	0.664099	2.9	2.5	8.15	609.2	19.3	0.0193994
19	8.45	682	85.8	0.0466988	6	0.48005	2.1	1.8	8.45	682	5.9	0.045681
20	8.65	731	91.9	0.050474	7	0.641754	2.8	2.4	8.65	727.8	7.1	0.0506471
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	776	97.6	0.0276049	7	0.295208	1.3	1.1	9.3	774.3	6.5	0.0255372

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.369	14	1.8	0.382677	5	2.97781	13.1	10.6	4.369	14.1	4.6	0.366045
2	4.731	59	7.4	0.168786	23	6.28757	27.6	22.4	4.731	58.7	22.7	0.156568
3	5.374	146	18.4	0.403873	3	1.90471	8.3	6.8	5.374	146.1	2.7	0.402508
4	5.423	154	19.4	0.387877	3	1.98485	8.7	7.1	5.423	153.4	2.9	0.384527
5	5.551	175	22	0.264026	3	1.23232	5.4	4.4	5.551	175.3	3.1	0.223322
6	5.606	184	23.1	0.0870586	11	1.64845	7.2	5.9	5.606	184.8	11.3	0.0823255
7	5.697	199	25	0.0923197	4	0.461923	2	1.6	5.697	199.6	3.8	0.0693905
8	5.795	215	27	0.197259	2	0.725	3.2	2.6	5.795	215.1	2.2	0.186869
9	6.242	308	38.7	0.212853	7	2.15125	9.4	7.7	6.242	308.4	7.5	0.162467
10	6.317	324	40.8	0.155294	10	2.79057	12.2	10	6.317	323.8	10.3	0.153505
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.485	360	45.3	0.0337694	9	0.438542	1.9	1.6	6.485	359.9	9.1	0.0272372
12	6.545	373	46.9	0.0253253	6	0.212738	0.9	0.8	6.545	373.9	5.6	0.0213327

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	33	4.2	0.102262	6	1.05987	10.8	8.2	4.55	34	6.2	0.0968986
2	5.2	117	14.8	0.0827432	6	0.793066	8.1	6.1	5.2	117	5.5	0.0807093
3	5.85	223	28.2	0.154731	4	1.05328	10.7	8.1	5.85	222.9	3.9	0.154137
4	6.55	372	47	0.149396	6	1.53496	15.6	11.9	6.55	371.2	6.2	0.139215
5	6.85	406	51.3	0.0363294	6	0.323807	3.3	2.5	6.85	406.6	5.6	0.0327159
6	7.35	481	60.7	0.12987	4	0.828636	8.4	6.4	7.35	480.8	3.8	0.123514
7	8.15	609	76.9	0.112039	7	1.32949	13.5	10.3	8.15	609.2	6.9	0.108806
8	8.45	678	85.6	0.120644	7	1.47944	15	11.4	8.45	677.7	7.1	0.118122
9	8.65	728	91.9	0.123926	4	0.839412	8.5	6.5	8.65	727.4	4.1	0.116572
10	9.3	775	97.9	0.0649122	6	0.606287	6.2	4.7	9.3	773.7	6.1	0.0564235

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 75

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.200 5.200 8.700 5.850 11.200 6.550 18.300 6.850 2.600 7.350 7.400 8.150 13.200 8.450 13.800 8.650 7.700 9.300 6.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.361 11.900 4.695 21.500 5.336 9.900 5.379 11.300 5.520 5.900 5.600 4.500 5.687 0.800 5.798 3.800 6.223 13.800 6.287 12.700 6.452 3.100 6.520 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 12 Return to Lane Navigator
4.550 10.500 5.200 9.600 5.850 12.300 6.550 17.500 6.850 3.300 7.350 6.400 8.150 13.100 8.450 13.100 8.650 7.700 9.300 6.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.358 10.300 4.550 5.200 4.708 22.100 5.200 1.100 5.358 2.900 5.382 11.500 5.534 4.600 5.595 5.400 5.698 2.700 5.807 2.600 5.850 1.400 6.244 5.200 6.323 9.100 6.494 2.400 6.550 3.300 6.850 0.500 7.350 0.700 8.150 2.900 8.450 2.100 8.650 2.800 9.300 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
4.369 13.100 4.731 27.600 5.374 8.300 5.423 8.700 5.551 5.400 5.606 7.200 5.697 2.000 5.795 3.200 6.242 9.400 6.317 12.200 6.485 1.900 6.545 0.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 12 Return to Lane Navigator
4.550 10.800 5.200 8.100 5.850 10.700 6.550 15.600 6.850 3.300 7.350 8.400 8.150 13.500 8.450 15.000 8.650 8.500 9.300 6.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Rex Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Rex Sole ---- Go to Display Gel Lane Navigator.