RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes pinniger
FDA Seafood List Common Name: Canary Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19930818gel03
Unique Web Code: cah02Srr
Tissue Aliquot Code: sebapinn 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	47	5.8	0.114229	5	0.909936	7	5.6	4.55	47.2	4.8	0.107548
2	5.2	143	17.6	0.210909	4	1.39819	10.7	8.6	5.2	143.5	3.8	0.205568
3	5.85	254	31.3	0.242408	4	1.6883	12.9	10.4	5.85	254	4	0.236305
4	6.55	387	47.7	0.208149	5	1.90648	14.6	11.7	6.55	386.2	5.5	0.196922
5	6.85	415	51.1	0.0362355	4	0.273552	2.1	1.7	6.85	414.6	4.4	0.0354532
6	7.35	480	59.1	0.0983744	4	0.672246	5.1	4.1	7.35	479.6	3.8	0.100005
7	8.15	604	74.4	0.130265	6	1.45274	11.1	8.9	8.15	603.7	6.2	0.13195
8	8.45	671	82.6	0.094103	8	1.35004	10.3	8.3	8.45	669.6	8	0.095482
9	8.65	719	88.5	0.094914	4	0.68017	5.2	4.2	8.65	718.3	4.1	0.09341
10	9.3	784	96.6	0.286019	5	2.75407	21	17	9.3	782.9	5.5	0.284747

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.366	24	3	0.020667	9	0.268648	1.4	1.1	4.366	24.8	9	0.0168971
2	4.55	52	6.4	0.0551032	3	0.327924	1.6	1.3	4.55	52	3.4	0.0550121
3	4.662	69	8.5	0.287302	5	2.35024	11.8	9.3	4.662	69.2	4.7	0.279267
4	4.957	114	14	0.0615049	4	0.455753	2.3	1.8	4.957	112.5	4.5	0.0573237
5	5.003	121	14.9	0.0609477	5	0.427114	2.1	1.7	5.003	122.1	4.5	0.0532504
6	5.147	143	17.6	0.0164986	4	0.143976	0.7	0.6	5.147	141.9	4.4	0.0184964
7	5.2	151	18.6	0.0819471	4	0.504222	2.5	2	5.2	150.6	3.5	0.081008
8	5.85	256	31.5	0.0919333	3	0.515337	2.6	2	5.85	255.5	3.1	0.0946439
9	5.909	267	32.9	0.472513	3	2.44943	12.3	9.7	5.909	267.2	3	0.467399
10	5.962	277	34.1	0.366148	3	1.59781	8	6.3	5.962	276.4	2.7	0.336196
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.994	283	34.9	0.165436	5	1.30157	6.5	5.1	5.994	283.1	4.8	0.153563
12	6.106	304	37.4	0.112712	3	0.555062	2.8	2.2	6.106	304.4	2.7	0.117767
13	6.144	311	38.3	0.100544	3	0.521018	2.6	2.1	6.144	312	2.7	0.110243
14	6.181	318	39.2	0.474514	2	1.56965	7.9	6.2	6.181	317.9	1.9	0.461571
15	6.31	342	42.1	0.0616017	4	0.469486	2.4	1.9	6.31	342	4.3	0.0614573
16	6.379	355	43.7	0.0207887	2	0.0523081	0.3	0.2	6.379	355.3	1.5	0.0192105
17	6.432	365	45	0.069003	2	0.224611	1.1	0.9	6.432	364.5	2	0.0625138
18	6.465	371	45.7	0.0787697	4	0.514845	2.6	2	6.465	370.9	4.3	0.0683228
19	6.55	387	47.7	0.0193622	2	0.0882033	0.4	0.3	6.55	386.9	2.4	0.0206479
20	6.85	414	51	0.0190022	5	0.179382	0.9	0.7	6.85	414.6	5.4	0.018692
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.361	481	59.2	0.0486178	6	0.462105	2.3	1.8	7.361	482.4	5.6	0.046907
22	7.534	508	62.6	0.0702861	11	1.31231	6.6	5.2	7.534	502.8	10.9	0.0681887
23	7.644	525	64.7	0.178256	3	0.886534	4.5	3.5	7.644	525.5	2.9	0.170444
24	8.45	671	82.6	0.0437792	7	0.487119	2.4	1.9	8.45	671.7	6.7	0.0412742
25	8.65	719	88.5	0.0391985	5	0.328297	1.7	1.3	8.65	717.7	4.8	0.0386177
26	9.3	785	96.7	0.171685	6	1.89003	9.5	7.5	9.3	784.6	6.2	0.17242

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.39	28	3.4	0.032329	8	0.410381	2.3	1.4	4.39	30.4	7.9	0.0292592
2	4.69	73	9	0.284387	7	3.05461	16.8	10.5	4.69	74.3	6.7	0.257632
3	4.983	117	14.4	0.0722282	3	0.130335	0.7	0.4	4.983	114.7	2.5	0.0288712
4	5.017	122	15	0.0822693	7	0.971186	5.3	3.3	5.017	121.2	7.1	0.0766496
5	5.163	144	17.7	0.0285369	3	0.142886	0.8	0.5	5.163	144.2	3	0.0271259
6	5.926	268	33	0.4984	3	2.68208	14.7	9.2	5.926	268.8	3	0.504476
7	5.979	278	34.2	0.398681	3	1.82349	10	6.3	5.979	277.5	2.7	0.383918
8	6.021	286	35.2	0.156654	4	1.23575	6.8	4.2	6.021	284.6	3.6	0.191628
9	6.121	305	37.6	0.13678	3	0.700644	3.8	2.4	6.121	305.1	2.8	0.140943
10	6.163	313	38.5	0.110483	3	0.536893	3	1.8	6.163	312.9	2.8	0.108792
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.195	319	39.3	0.462563	2	1.48888	8.2	5.1	6.195	318.7	1.8	0.466685
12	6.316	342	42.1	0.0881837	5	0.779239	4.3	2.7	6.316	342.8	5.1	0.0869139
13	6.384	355	43.7	0.0473285	3	0.268647	1.5	0.9	6.384	355	3.5	0.0436137
14	6.437	365	45	0.0874859	2	0.35401	1.9	1.2	6.437	364.2	2.3	0.0862724
15	6.463	370	45.6	0.107653	4	0.654546	3.6	2.2	6.463	370.3	3.5	0.104833
16	7.391	485	59.7	0.0305657	3	0.150939	0.8	0.5	7.391	485.6	3.1	0.0271783
17	7.538	508	62.6	0.097346	10	1.61983	8.9	5.6	7.538	504.8	10	0.0910661
18	7.647	525	64.7	0.222786	3	1.19531	6.6	4.1	7.647	525.5	3	0.222596

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	52	6.4	0.0675606	5	0.502658	5.2	4	4.55	52.7	4.6	0.0616545
2	5.2	148	18.2	0.156592	4	0.963372	9.9	7.6	5.2	147.6	3.5	0.15371
3	5.85	251	30.9	0.202255	4	1.39838	14.3	11	5.85	251.5	3.9	0.202176
4	6.55	386	47.5	0.16439	5	1.4202	14.6	11.2	6.55	385.8	5.2	0.155068
5	6.85	414	50.9	0.023349	4	0.164778	1.7	1.3	6.85	415.1	4.3	0.0217698
6	7.35	478	58.8	0.0617761	4	0.412569	4.2	3.3	7.35	478.1	3.8	0.0614828
7	8.15	603	74.2	0.109311	6	1.11271	11.4	8.8	8.15	602.3	5.7	0.111041
8	8.45	665	81.8	0.0746062	7	0.927874	9.5	7.3	8.45	665	7.1	0.0733009
9	8.65	716	88.1	0.0728837	4	0.5036	5.2	4	8.65	715.6	3.9	0.0721391
10	9.3	782	96.2	0.244447	5	2.34079	24	18.4	9.3	781.2	5.4	0.243435

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.389	30	3.7	0.0169228	9	0.337257	1.7	1.2	4.389	35.1	8.7	0.0219586
2	4.55	55	6.8	0.0682836	3	0.41536	2.1	1.5	4.55	55.4	3.4	0.0685179
3	4.666	73	9	0.225739	6	2.28628	11.4	8.4	4.666	74.3	6	0.213992
4	4.936	115	14.1	0.0670746	8	0.739928	3.7	2.7	4.936	112.2	7.7	0.0538692
5	4.968	120	14.8	0.090837	5	0.610369	3.1	2.2	4.968	120.9	4.7	0.0737538
6	5.129	145	17.8	0.0197538	5	0.1658	0.8	0.6	5.129	145.9	4.7	0.0199549
7	5.2	156	19.2	0.0931485	4	0.641018	3.2	2.4	5.2	155.3	3.9	0.0925312
8	5.85	256	31.5	0.0912153	3	0.499089	2.5	1.8	5.85	255.7	3.1	0.0914379
9	5.907	267	32.8	0.420516	3	2.22873	11.1	8.2	5.907	267.2	3	0.414859
10	5.96	277	34.1	0.318217	3	1.43944	7.2	5.3	5.96	276.2	2.8	0.285098
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.996	284	34.9	0.126134	5	1.19229	6	4.4	5.996	282.5	5.2	0.128388
12	6.101	304	37.4	0.0983977	3	0.505295	2.5	1.9	6.101	304.3	2.9	0.0992549
13	6.143	312	38.4	0.0924815	4	0.616415	3.1	2.3	6.143	313	4	0.0865649
14	6.174	318	39.1	0.403245	2	1.27998	6.4	4.7	6.174	318.2	1.9	0.380541
15	6.304	343	42.2	0.056041	5	0.492225	2.5	1.8	6.304	343.1	5.1	0.0548195
16	6.383	358	44	0.0178363	2	0.0452694	0.2	0.2	6.383	357.5	1.6	0.0155124
17	6.43	367	45.1	0.0621172	1	0.0279444	0.1	0.1	6.43	366.6	0.8	0.0208437
18	6.466	374	46	0.0897238	10	1.19006	6	4.4	6.466	374.1	9.5	0.0706256
19	6.55	390	48	0.052793	4	0.247904	1.2	0.9	6.55	390.5	3.7	0.037424
20	6.85	418	51.4	0.0157769	4	0.10702	0.5	0.4	6.85	417.7	4.1	0.0147328
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.378	484	59.5	0.0449546	6	0.413072	2.1	1.5	7.378	485	5.7	0.0411318
22	7.558	512	63	0.0558195	11	1.15986	5.8	4.3	7.558	505.5	11.5	0.0571402
23	7.667	529	65.1	0.159809	3	0.741452	3.7	2.7	7.667	529.2	2.7	0.153771
24	8.45	672	82.7	0.0451464	5	0.41043	2.1	1.5	8.45	671.8	5.1	0.0454178
25	8.65	720	88.6	0.03151	5	0.260381	1.3	1	8.65	719.7	4.8	0.0308522
26	9.3	783	96.3	0.182893	6	1.94099	9.7	7.1	9.3	782.7	6	0.181228

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.34	20	2.5	0.0303461	13	0.564614	2.7	2	4.34	22.3	12.6	0.0252907
2	4.64	65	8	0.298184	5	2.37274	11.5	8.3	4.64	65.9	5.1	0.262587
3	4.88	101	12.4	0.132142	18	2.90512	14	10.1	4.88	93.9	18	0.0910593
4	4.927	108	13.3	0.121504	9	0.933197	4.5	3.3	4.927	106.6	9.3	0.0568516
5	5.1	134	16.5	0.0273301	4	0.146039	0.7	0.5	5.1	135.5	4	0.0204404
6	5.892	264	32.5	0.486262	3	2.58629	12.5	9	5.892	264.6	3	0.481836
7	5.939	273	33.6	0.390769	3	1.97777	9.6	6.9	5.939	273.2	2.9	0.379519
8	5.982	281	34.6	0.186502	4	1.38455	6.7	4.8	5.982	281.7	4.2	0.183815
9	6.092	302	37.2	0.126593	3	0.708495	3.4	2.5	6.092	302.1	3.1	0.130099
10	6.129	309	38.1	0.0960239	3	0.530142	2.6	1.9	6.129	310.1	2.8	0.105565
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.166	316	38.9	0.420859	2	1.52782	7.4	5.3	6.166	315.6	2	0.431385
12	6.287	339	41.7	0.0827492	5	0.764202	3.7	2.7	6.287	340.2	5.2	0.0828816
13	6.355	352	43.3	0.0400397	3	0.225775	1.1	0.8	6.355	352.3	3.3	0.0380721
14	6.413	363	44.7	0.0778856	3	0.355903	1.7	1.2	6.413	361.7	2.7	0.0736223
15	6.445	369	45.4	0.104242	4	0.704199	3.4	2.5	6.445	368.4	4	0.0995706
16	7.393	487	60	0.0288369	2	0.120904	0.6	0.4	7.393	486.9	2.4	0.028125
17	7.535	509	62.7	0.100001	9	1.66696	8.1	5.8	7.535	505.3	9.3	0.100958
18	7.652	527	64.9	0.234424	3	1.22735	5.9	4.3	7.652	526.7	3	0.233797

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	49	6	0.0978401	7	1.08264	8.1	6.7	4.55	49.8	6.7	0.0907575
2	5.2	143	17.6	0.111644	9	1.6446	12.3	10.1	5.2	146.5	8.6	0.108108
3	5.85	257	31.7	0.166794	6	1.75462	13.1	10.8	5.85	256.9	5.8	0.169626
4	6.55	389	47.9	0.196229	6	1.92057	14.4	11.8	6.55	388.1	5.8	0.185821
5	6.85	418	51.5	0.0288548	5	0.228574	1.7	1.4	6.85	418.3	4.9	0.0262859
6	7.35	482	59.4	0.0867508	4	0.580263	4.3	3.6	7.35	482.1	3.8	0.0858391
7	8.15	605	74.5	0.129671	7	1.52297	11.4	9.4	8.15	605.3	6.5	0.131873
8	8.45	667	82.1	0.0994801	7	1.12639	8.4	6.9	8.45	667	6.6	0.0962911
9	8.65	715	88.1	0.0641163	6	0.586633	4.4	3.6	8.65	715.6	5.6	0.0586092
10	9.3	781	96.2	0.283504	6	2.90636	21.8	17.9	9.3	780.1	5.9	0.277457

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 118

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.000 5.200 10.700 5.850 12.900 6.550 14.600 6.850 2.100 7.350 5.100 8.150 11.100 8.450 10.300 8.650 5.200 9.300 21.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.366 1.400 4.550 1.600 4.662 11.800 4.957 2.300 5.003 2.100 5.147 0.700 5.200 2.500 5.850 2.600 5.909 12.300 5.962 8.000 5.994 6.500 6.106 2.800 6.144 2.600 6.181 7.900 6.310 2.400 6.379 0.300 6.432 1.100 6.465 2.600 6.550 0.400 6.850 0.900 7.361 2.300 7.534 6.600 7.644 4.500 8.450 2.400 8.650 1.700 9.300 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 3 PeakCount: 26 Return to Lane Navigator
4.390 2.300 4.690 16.800 4.983 0.700 5.017 5.300 5.163 0.800 5.926 14.700 5.979 10.000 6.021 6.800 6.121 3.800 6.163 3.000 6.195 8.200 6.316 4.300 6.384 1.500 6.437 1.900 6.463 3.600 7.391 0.800 7.538 8.900 7.647 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: Specimen ACTUAL GEL LANE: 4 PeakCount: 18 Return to Lane Navigator
4.550 5.200 5.200 9.900 5.850 14.300 6.550 14.600 6.850 1.700 7.350 4.200 8.150 11.400 8.450 9.500 8.650 5.200 9.300 24.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: pI Markers ACTUAL GEL LANE: 5 PeakCount: 10 Return to Lane Navigator
4.389 1.700 4.550 2.100 4.666 11.400 4.936 3.700 4.968 3.100 5.129 0.800 5.200 3.200 5.850 2.500 5.907 11.100 5.960 7.200 5.996 6.000 6.101 2.500 6.143 3.100 6.174 6.400 6.304 2.500 6.383 0.200 6.430 0.100 6.466 6.000 6.550 1.200 6.850 0.500 7.378 2.100 7.558 5.800 7.667 3.700 8.450 2.100 8.650 1.300 9.300 9.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 6 PeakCount: 26 Return to Lane Navigator
4.340 2.700 4.640 11.500 4.880 14.000 4.927 4.500 5.100 0.700 5.892 12.500 5.939 9.600 5.982 6.700 6.092 3.400 6.129 2.600 6.166 7.400 6.287 3.700 6.355 1.100 6.413 1.700 6.445 3.400 7.393 0.600 7.535 8.100 7.652 5.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 7 PeakCount: 18 Return to Lane Navigator
4.550 8.100 5.200 12.300 5.850 13.100 6.550 14.400 6.850 1.700 7.350 4.300 8.150 11.400 8.450 8.400 8.650 4.400 9.300 21.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 10 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Canary Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.