Solution structure of a protein inhibitor of neuro...[Nat Struct Biol. 1998]

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1: Nat Struct Biol. 1998 Nov;5(11):965-9.Links

Solution structure of a protein inhibitor of neuronal nitric oxide synthase.

Tochio H, Ohki S, Zhang Q, Li M, Zhang M.

Department of Biochemistry, The Hong Kong University of Science and Technology, Kowloon, P.R. China.

The structure of the neuronal nitric oxide synthase inhibitory protein, PIN (protein inhibitor of nNOS), has been determined by NMR spectroscopy. Two N-terminal antiparallel alpha-helices pack against a four-stranded antiparallel beta-sheet in the C-terminal region of the protein, forming a two-layer alpha/beta plait. The three dimensional structure of PIN resembles the fold of the B-chain of aspartylglucosaminidase. A non-prolyl cis peptide bond was found between Pro 52 and Thr 53 of the protein. PIN has a large solvent-exposed hydrophobic surface that contains a cavity and is rimmed with positive charges. This surface may serve as the primary target-binding region for this multi-functional regulatory protein.

PMID: 9808041 [PubMed - indexed for MEDLINE]

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Solution structure of a protein inhibitor of neuronal nitric oxide synthase.Solution structure of a protein inhibitor of neuronal nitric oxide synthase.

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