PLOY MC, LAMBERT T, DENIS F, COURVALIN P; Interscience Conference on Antimicrobial Agents and Chemotherapy.
Abstr Intersci Conf Antimicrob Agents Chemother Intersci Conf Antimicrob Agents Chemother. 1999 Sep 26-29; 39: 114 (abstract no. 836).
Inst. Pasteur / CHU Dupuytren, Paris / Limoges, FRANCE
The aac(6')-Iz resistance gene of Stenotrophomonas maltophilia BM2690 encoding an aminoglycoside 6'-N-acetyltransferase [AAC(6')-Iz] was characterized. The gene was identified as a coding sequence of 462 bp corresponding to a protein with a calculated mass of 16,506 Da, a value in good agreement with that of ca. 16,000 found by in vitro coupled transcription-translation. Analysis of the deduced amino acid sequence indicated that the protein was a member of the major subfamily of AAC(6'). The ORF upstream from the aac(6')-Iz gene was homologous to the fprA gene of Myxococcus xanthus (61% identity) which encodes a putative pyridoxine (pyridoxamine) 5'-phosphate oxidase. Pulsed-field gel electrophoresis of total DNA from BM2690 and S. maltophilia ATTC13637 digested with XbaI, DraI, and SpeI, followed by hybridization with rRNA and aac(6')-Iz specific probes indicated that the gene was located in the chromosome. The aac(6')-Iz gene was detected by DNA-DNA hybridization in all eighty strains of S. maltophilia tested. The MICs of gentamicin against these strains were lower than those of amikacin, netilmicin, and tobramycin, indicating that production of AAC(6')-Iz contributes to aminoglycoside resistance in S. maltophilia.
Publication Types:
Keywords:
- Acetyltransferases
- Alginates
- Aluminum Hydroxide
- Amikacin
- Amino Acid Sequence
- Aminoglycosides
- Anti-Bacterial Agents
- Base Sequence
- Drug Combinations
- Electrophoresis, Gel, Pulsed-Field
- In Vitro
- Microbial Sensitivity Tests
- Sodium Bicarbonate
- Stenotrophomonas maltophilia
- alginic acid compound
- aminoglycoside N(6')-acetyltransferase
- genetics
Other ID:
UI: 102244860
From Meeting Abstracts