Skip Nav

An organized view of the transcripome



Library:10769 (dbEST ID)

MT0429

Library Description

Organism:Homo sapiens
Developmental stage:Adult
Organ:marrow
Vector:puc18
Vector type:Plasmid
Cloning site 1:SmaI
Cloning site 2:SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

69 ESTs from this library were grouped into 63 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
3 43478 Hs.524579 Lysozyme (renal amyloidosis).
3 43478 Hs.520640 Actin, beta.
3 43478 Hs.204238 Lipocalin 2.
1 14493 Hs.83636 Adrenergic, beta, receptor kinase 1.
1 14493 Hs.7744 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa.
1 14493 Hs.75516 Tyrosine kinase 2.
1 14493 Hs.753 Formyl peptide receptor 1.
1 14493 Hs.713560 ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast).
1 14493 Hs.708811 Transcribed locus.
1 14493 Hs.696221 Zinc finger protein 655.
1 14493 Hs.673372 Transcribed locus, strongly similar to NP_001129575.1 transmembrane protein 132C [Homo sapiens].
1 14493 Hs.659260 Transcribed locus, strongly similar to NP_001851.1 solute carrier family 31 (copper transporters), member 2 [Homo sapiens].
1 14493 Hs.659185 Transcribed locus.
1 14493 Hs.655552 ArfGAP with SH3 domain, ankyrin repeat and PH domain 1.
1 14493 Hs.654404 Major histocompatibility complex, class I, C.
1 14493 Hs.647039 GTF2I repeat domain containing 2.
1 14493 Hs.636561 Hypothetical protein LOC200030.
1 14493 Hs.632373 Glucocorticoid modulatory element binding protein 1.
1 14493 Hs.632242 Calmodulin binding transcription activator 2.
1 14493 Hs.631932 Similar to hypothetical protein LOC284701.
1 14493 Hs.596014 Importin 9.
1 14493 Hs.591366 Coiled-coil domain containing 109A.
1 14493 Hs.576612 Ectonucleoside triphosphate diphosphohydrolase 1.
1 14493 Hs.568340 DENN/MADD domain containing 1A.
1 14493 Hs.553221 IKAROS family zinc finger 4 (Eos).
1 14493 Hs.534312 Torsin family 1, member A (torsin A).
1 14493 Hs.529488 Solute carrier family 6 (neurotransmitter transporter, taurine), member 6.
1 14493 Hs.528605 PP12104.
1 14493 Hs.522384 F-box protein 42.
1 14493 Hs.518417 Syntaxin 6.
1 14493 Hs.512651 Ataxin 7-like 3.
1 14493 Hs.466471 Glucose phosphate isomerase.
1 14493 Hs.461819 Vacuolar protein sorting 53 homolog (S. cerevisiae).
1 14493 Hs.461200 Clone HLS_IMAGE_136933 mRNA sequence.
1 14493 Hs.460238 SH3-domain GRB2-like endophilin B2.
1 14493 Hs.460124 PHD finger protein 19.
1 14493 Hs.458272 Myeloperoxidase.
1 14493 Hs.440898 Ficolin (collagen/fibrinogen domain containing) 1.
1 14493 Hs.440497 Potassium voltage-gated channel, shaker-related subfamily, beta member 2.
1 14493 Hs.440382 Tripartite motif-containing 27.
1 14493 Hs.436093 HLA-B associated transcript 2.
1 14493 Hs.43322 Protein kinase, AMP-activated, alpha 1 catalytic subunit.
1 14493 Hs.408458 WW domain containing E3 ubiquitin protein ligase 2.
1 14493 Hs.386225 Glycogen synthase 1 (muscle).
1 14493 Hs.368610 3'-phosphoadenosine 5'-phosphosulfate synthase 1.
1 14493 Hs.365286 Family with sequence similarity 21, member C.
1 14493 Hs.34871 Zinc finger E-box binding homeobox 2.
1 14493 Hs.334637 Hydrogen voltage-gated channel 1.
1 14493 Hs.270291 Actinin, alpha 4.
1 14493 Hs.262857 Prune homolog 2 (Drosophila).
1 14493 Hs.254042 HLA-B associated transcript 1.
1 14493 Hs.249441 WEE1 homolog (S. pombe).
1 14493 Hs.24301 Polymerase (RNA) II (DNA directed) polypeptide E, 25kDa.
1 14493 Hs.234742 Lactoperoxidase.
1 14493 Hs.195464 Filamin A, alpha (actin binding protein 280).
1 14493 Hs.181244 Major histocompatibility complex, class I, A.
1 14493 Hs.144725 GRAM domain containing 1B.
1 14493 Hs.128420 Vacuolar protein sorting 4 homolog A (S. cerevisiae).
1 14493 Hs.127022 Protein tyrosine phosphatase, receptor type, E.
1 14493 Hs.126517 CD55 molecule, decay accelerating factor for complement (Cromer blood group).
1 14493 Hs.118076 Ribosomal protein S4, X-linked.
1 14493 Hs.113287 Discs, large (Drosophila) homolog-associated protein 2.
1 14493 Hs.106019 Protein phosphatase 1, regulatory (inhibitor) subunit 10.

Source: NCBI UniGene

EST Sequences

132 EST sequences from this library have been submitted to the public sequence database as of 05-Jan-2009.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • blood
  • leukemia
  • adult
  • flow sorted

Source: NCBI Clone Registry

References

Shotgun sequencing of the human transcriptome with ORF expressed sequence tags. Dias Neto E, et al., Proc Natl Acad Sci U S A 97, 3491-3496 (2000).



National Center for Biotechnology Information
U.S. National Library of Medicine
8600 Rockville Pike, Bethesda, MD 20894