RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus macrocephalus
FDA Seafood List Common Name: Pacific Cod
FDA Seafood List Market Name: Cod (Alaska Cod)
Unique IEF Gel Code: 19930816gel06
Unique Web Code: pch02Srr
Tissue Aliquot Code: gadumacr 003 #2 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 1
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	49	6.1	0.0828162	4	0.569578	5.8	4.7	4.55	49.1	4	0.0805644
2	5.2	146	18.2	0.169341	4	1.0756	10.9	8.9	5.2	145.6	3.6	0.169033
3	5.85	248	31	0.174485	4	1.35549	13.8	11.2	5.85	248	4.4	0.175578
4	6.55	385	48.1	0.144147	6	1.47461	15	12.2	6.55	384.1	5.9	0.140511
5	6.85	414	51.8	0.0322933	8	0.409482	4.2	3.4	6.85	414.8	8.1	0.0285081
6	7.35	478	59.8	0.0735978	4	0.515385	5.2	4.3	7.35	478.2	3.9	0.0749507
7	8.15	605	75.6	0.0937812	7	1.09058	11.1	9	8.15	604.3	6.5	0.0945248
8	8.45	667	83.4	0.0870521	7	1.058	10.7	8.7	8.45	667.1	6.8	0.087788
9	8.65	718	89.8	0.0707863	5	0.556608	5.7	4.6	8.65	718.1	4.5	0.0693989
10	9.3	775	96.9	0.185406	5	1.74156	17.7	14.4	9.3	775.1	5.3	0.186759

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.481	37	4.6	0.0759897	6	0.759744	2.7	2.3	4.481	35.2	6	0.0711394
2	4.634	60	7.5	0.194874	20	6.80262	24.4	20.3	4.634	64.9	20.5	0.18734
3	5.12	133	16.6	0.0142843	2	0.045736	0.2	0.1	5.12	133.2	1.8	0.0142586
4	5.2	145	18.1	0.072563	4	0.436379	1.6	1.3	5.2	144.4	3.5	0.0696138
5	5.283	158	19.7	0.53679	3	2.95532	10.6	8.8	5.283	158.4	3.1	0.532974
6	5.423	180	22.5	0.305385	3	1.65707	6	4.9	5.423	179.5	3.1	0.303537
7	5.716	226	28.2	0.420208	3	2.39434	8.6	7.1	5.716	226.4	3.4	0.392947
8	5.799	239	29.8	0.144994	10	2.26893	8.2	6.8	5.799	238.5	9.6	0.133625
9	5.85	247	30.8	0.0846738	3	0.127049	0.5	0.4	5.85	249	3.1	0.0228656
10	5.952	267	33.3	0.0207807	3	0.117413	0.4	0.3	5.952	267.6	3.4	0.0197644
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.034	283	35.3	0.0542914	9	0.848404	3	2.5	6.034	285.7	9	0.0530365
12	6.1	296	37	0.258191	2	0.901016	3.2	2.7	6.1	296.1	2.1	0.237801
13	6.203	316	39.5	0.147728	4	1.10978	4	3.3	6.203	314.5	4.1	0.152311
14	6.279	331	41.3	0.105308	5	0.927293	3.3	2.8	6.279	331.4	5.1	0.102075
15	6.412	357	44.6	0.0493986	10	0.907205	3.3	2.7	6.412	361.4	10.2	0.0503316
16	6.55	384	47.9	0.0230406	10	0.40321	1.4	1.2	6.55	377.9	9.6	0.0237893
17	6.85	410	51.2	0.0291155	4	0.212326	0.8	0.6	6.85	409.4	4.3	0.0280021
18	7.35	476	59.4	0.114339	7	1.34328	4.8	4	7.35	475.9	6.9	0.110166
19	7.461	493	61.5	0.0364465	13	0.619447	2.2	1.8	7.461	495.7	12.6	0.0277584
20	8.15	599	74.8	0.0423661	9	0.595697	2.1	1.8	8.15	601.1	9	0.0373328
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	663	82.8	0.0582803	8	0.802804	2.9	2.4	8.45	663.5	8	0.0567722
22	8.65	715	89.3	0.0432802	4	0.309278	1.1	0.9	8.65	714.6	4.2	0.0416894
23	9.3	776	96.9	0.104333	8	1.28919	4.6	3.8	9.3	775.5	7.5	0.096921

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.479	35	4.4	0.0510391	4	0.320242	1.4	1	4.479	34.4	3.7	0.0488524
2	4.625	57	7.1	0.242303	17	7.13032	32.1	21.2	4.625	58.4	17.1	0.234708
3	5.124	132	16.5	0.0036074	3	0.0270596	0.1	0.1	5.124	133.3	3.1	0.0048851
4	5.19	142	17.7	0.0139721	3	0.0593085	0.3	0.2	5.19	141.6	2.6	0.0130121
5	5.3	159	19.9	0.374141	5	3.10531	14	9.2	5.3	158.6	4.8	0.36691
6	5.441	181	22.6	0.240736	4	1.7909	8.1	5.3	5.441	180	4.4	0.230494
7	5.731	226	28.2	0.297769	6	2.80798	12.7	8.3	5.731	224.8	5.7	0.279941
8	5.815	239	29.8	0.125885	8	1.71568	7.7	5.1	5.815	236.8	7.6	0.128006
9	5.933	261	32.6	0.0208347	12	0.497044	2.2	1.5	5.933	254.7	11.6	0.0242711
10	6.034	281	35.1	0.0949486	8	1.28332	5.8	3.8	6.034	284.4	7.6	0.0954079
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.095	293	36.6	0.137747	3	0.586781	2.6	1.7	6.095	292.8	3.1	0.107696
12	6.176	309	38.6	0.116582	5	0.977362	4.4	2.9	6.176	309.8	4.7	0.117725
13	6.267	327	40.8	0.0978091	7	1.11961	5	3.3	6.267	328.2	6.8	0.0931754
14	6.408	355	44.3	0.0577205	9	0.772277	3.5	2.3	6.408	358.6	9.2	0.0474394

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	44	5.5	0.0894612	5	0.712739	6.4	5.1	4.55	43.4	4.6	0.0872575
2	5.2	142	17.7	0.178688	4	1.23703	11	8.9	5.2	141.6	4	0.176324
3	5.85	242	30.2	0.182765	4	1.39474	12.5	10	5.85	242.4	4.4	0.180488
4	6.55	382	47.6	0.182913	5	1.63984	14.6	11.8	6.55	381.4	5.3	0.173652
5	6.85	411	51.2	0.030306	5	0.266225	2.4	1.9	6.85	410.5	5.3	0.0282569
6	7.35	476	59.4	0.0892192	4	0.686807	6.1	4.9	7.35	475.6	4.3	0.0899088
7	8.15	598	74.6	0.119537	6	1.35123	12.1	9.7	8.15	597.5	6.4	0.118406
8	8.45	665	82.9	0.13024	6	1.37357	12.3	9.9	8.45	664.8	6	0.129694
9	8.65	714	89	0.0984825	4	0.711383	6.4	5.1	8.65	713.9	4.2	0.0966469
10	9.3	770	96	0.193696	5	1.8266	16.3	13.1	9.3	769.4	5.3	0.192942

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.466	31	3.9	0.0709454	1	0	0	0	4.466	18	1	0
2	4.606	51	6.4	0.20232	21	7.2633	28.8	22.9	4.606	55.3	20.9	0.196416
3	5.123	125	15.6	0.0069425	2	0.0247699	0.1	0.1	5.123	124.1	2.3	0.0060831
4	5.2	136	17	0.0581137	3	0.321944	1.3	1	5.2	134.9	3.2	0.0568434
5	5.271	148	18.5	0.441977	3	2.65062	10.5	8.4	5.271	148.4	3.4	0.443824
6	5.401	170	21.2	0.255503	4	1.6368	6.5	5.2	5.401	169.9	3.7	0.249698
7	5.708	222	27.7	0.340919	4	2.51776	10	7.9	5.708	222.2	4.4	0.324461
8	5.797	237	29.6	0.121397	9	1.81889	7.2	5.7	5.797	236.5	9	0.114305
9	5.85	246	30.7	0.0669857	3	0.18221	0.7	0.6	5.85	246.7	3.3	0.0313806
10	5.949	265	33	0.0209172	3	0.116842	0.5	0.4	5.949	264.7	3.4	0.0192447
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.017	278	34.7	0.0677007	8	0.887734	3.5	2.8	6.017	280.2	8.1	0.0621261
12	6.09	292	36.4	0.210631	2	0.829445	3.3	2.6	6.09	291.8	2.4	0.197449
13	6.178	309	38.5	0.137994	4	0.9164	3.6	2.9	6.178	308.3	3.6	0.144957
14	6.262	325	40.5	0.0938987	6	0.96609	3.8	3	6.262	325.5	6	0.0907449
15	6.413	354	44.1	0.0485644	12	0.993349	3.9	3.1	6.413	360.4	11.8	0.0474752
16	6.85	410	51.1	0.0198354	6	0.183482	0.7	0.6	6.85	409.5	5.8	0.0179837
17	7.35	475	59.2	0.102661	6	1.03094	4.1	3.3	7.35	475.2	5.8	0.100907
18	7.445	490	61.1	0.0267614	4	0.191176	0.8	0.6	7.445	490	4.1	0.0262109
19	8.15	601	74.9	0.0794576	7	0.886218	3.5	2.8	8.15	597.3	7.1	0.0704449
20	8.45	666	83	0.0431289	7	0.539198	2.1	1.7	8.45	665.5	7.3	0.041546
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	714	89	0.040262	4	0.288749	1.1	0.9	8.65	713.9	4.2	0.0389909
22	9.3	769	95.9	0.107114	5	0.962657	3.8	3	9.3	768.7	5.2	0.104369

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.463	29	3.6	0.0841687	6	0.864674	3.4	2.4	4.463	28.1	6.1	0.0804737
2	4.637	55	6.9	0.251586	18	7.15519	27.9	19.9	4.637	55.7	17.8	0.22671
3	5.097	124	15.5	0.0259958	24	3.05364	11.9	8.5	5.097	92.3	24	0.0717862
4	5.163	134	16.7	0.0275721	4	0.132276	0.5	0.4	5.163	133.7	3.9	0.0191466
5	5.265	150	18.7	0.395366	4	2.95107	11.5	8.2	5.265	149.5	4.1	0.402783
6	5.396	171	21.3	0.242828	4	1.73596	6.8	4.8	5.396	171	4.1	0.241615
7	5.701	220	27.4	0.39839	4	2.55712	10	7.1	5.701	219.9	3.8	0.375002
8	5.788	234	29.2	0.160695	9	2.23514	8.7	6.2	5.788	233.1	8.6	0.146673
9	5.923	258	32.2	0.0382324	9	0.439049	1.7	1.2	5.923	255.8	8.6	0.02872
10	6.011	275	34.3	0.0531186	7	0.729546	2.8	2	6.011	277.6	7.2	0.0572551
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.074	287	35.8	0.189294	3	0.972794	3.8	2.7	6.074	287.2	3.1	0.17545
12	6.183	308	38.4	0.131765	4	1.03325	4	2.9	6.183	306.6	4.5	0.129663
13	6.266	324	40.4	0.0929544	6	0.938204	3.7	2.6	6.266	325.4	5.9	0.0900949
14	6.422	354	44.1	0.0521435	10	0.830557	3.2	2.3	6.422	356.5	9.6	0.0487096

PeakCounter: 14

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	41	5.1	0.103972	5	0.831526	6.9	5.5	4.55	41.6	4.7	0.0995384
2	5.2	143	17.8	0.180621	4	1.24358	10.3	8.3	5.2	142.8	3.9	0.178021
3	5.85	242	30.2	0.188317	4	1.41769	11.8	9.4	5.85	242.1	4.3	0.187726
4	6.55	377	47	0.17008	5	1.48097	12.3	9.8	6.55	376.7	5.1	0.164467
5	6.85	407	50.7	0.0262456	6	0.246327	2	1.6	6.85	406.5	5.6	0.0246819
6	7.35	471	58.7	0.0923819	4	0.694468	5.8	4.6	7.35	471.2	4.3	0.0917399
7	8.15	593	73.9	0.106205	8	1.50588	12.5	10	8.15	593.6	8.4	0.101529
8	8.45	658	82	0.121539	7	1.59307	13.2	10.6	8.45	657.9	7.3	0.123622
9	8.65	711	88.7	0.10299	4	0.739645	6.1	4.9	8.65	711	4.1	0.101025
10	9.3	770	96	0.245415	5	2.30005	19.1	15.3	9.3	769.4	5.4	0.240551

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.800 5.200 10.900 5.850 13.800 6.550 15.000 6.850 4.200 7.350 5.200 8.150 11.100 8.450 10.700 8.650 5.700 9.300 17.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 1 PeakCount: 10 Return to Lane Navigator
4.481 2.700 4.634 24.400 5.120 0.200 5.200 1.600 5.283 10.600 5.423 6.000 5.716 8.600 5.799 8.200 5.850 0.500 5.952 0.400 6.034 3.000 6.100 3.200 6.203 4.000 6.279 3.300 6.412 3.300 6.550 1.400 6.850 0.800 7.350 4.800 7.461 2.200 8.150 2.100 8.450 2.900 8.650 1.100 9.300 4.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 2 PeakCount: 23 Return to Lane Navigator
4.479 1.400 4.625 32.100 5.124 0.100 5.190 0.300 5.300 14.000 5.441 8.100 5.731 12.700 5.815 7.700 5.933 2.200 6.034 5.800 6.095 2.600 6.176 4.400 6.267 5.000 6.408 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 14 Return to Lane Navigator
4.550 6.400 5.200 11.000 5.850 12.500 6.550 14.600 6.850 2.400 7.350 6.100 8.150 12.100 8.450 12.300 8.650 6.400 9.300 16.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.466 0 4.606 28.800 5.123 0.100 5.200 1.300 5.271 10.500 5.401 6.500 5.708 10.000 5.797 7.200 5.850 0.700 5.949 0.500 6.017 3.500 6.090 3.300 6.178 3.600 6.262 3.800 6.413 3.900 6.850 0.700 7.350 4.100 7.445 0.800 8.150 3.500 8.450 2.100 8.650 1.100 9.300 3.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.463 3.400 4.637 27.900 5.097 11.900 5.163 0.500 5.265 11.500 5.396 6.800 5.701 10.000 5.788 8.700 5.923 1.700 6.011 2.800 6.074 3.800 6.183 4.000 6.266 3.700 6.422 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 14 Return to Lane Navigator
4.550 6.900 5.200 10.300 5.850 11.800 6.550 12.300 6.850 2.000 7.350 5.800 8.150 12.500 8.450 13.200 8.650 6.100 9.300 19.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Cod ---- Go to Display Gel Lane Navigator.