CN0096

Library Description

Organism:	Homo sapiens
Developmental stage:	Adult
Organ:	colon_normal
Vector:	puc18
Vector type:	Plasmid
Cloning site 1:	SmaI
Cloning site 2:	SmaI

A mini-library was made by cloning products derived from ORESTES PCR (U.S. Letters Patent application No. 196,716 - Ludwig Institute for Cancer Research) profiles into the pUC 18 vector. Reverse transcription of tissue mRNA and cDNA amplification were performed under low stringency conditions.

Source: Simpson A.J.G., Ludwig Institute for Cancer Research

Gene Content Analysis

205 ESTs from this library were grouped into 129 UniGene entries (putative genes) [UniGene build #218, 05-Jan-2009]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs	TPM	UniGene Entry
28	136585	Hs.532793	Karyopherin (importin) beta 1.
5	24390	Hs.642564	Transcribed locus.
5	24390	Hs.504237	STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae).
5	24390	Hs.470417	Penta-EF-hand domain containing 1.
4	19512	Hs.659356	GIPC PDZ domain containing family, member 2.
4	19512	Hs.467192	Protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform.
3	14634	Hs.632287	Protein kinase, Y-linked.
3	14634	Hs.631925	Solute carrier family 26, member 6.
3	14634	Hs.518525	Glutamate-ammonia ligase (glutamine synthetase).
3	14634	Hs.516157	Methionine adenosyltransferase II, alpha.
3	14634	Hs.41752	Keratin 32.
3	14634	Hs.144835	Eukaryotic translation elongation factor 1 gamma.
2	9756	Hs.75514	Nucleoside phosphorylase.
2	9756	Hs.702010	DEAD (Asp-Glu-Ala-Asp) box polypeptide 42.
2	9756	Hs.695041	Transcribed locus, strongly similar to NP_036914.1 branched chain ketoacid dehydrogenase E1, alpha polypeptide [Rattus norvegicus].
2	9756	Hs.687000	Transcribed locus.
2	9756	Hs.670556	Transcribed locus.
2	9756	Hs.654800	Patatin-like phospholipase domain containing 3.
2	9756	Hs.592490	Fumarate hydratase.
2	9756	Hs.543850	TNFAIP3 interacting protein 1.
2	9756	Hs.523789	Non-protein coding RNA 84.
2	9756	Hs.522789	Family with sequence similarity 127, member A.
2	9756	Hs.517033	Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase).
2	9756	Hs.503165	ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1.
2	9756	Hs.463642	Ribosomal protein S6 kinase, 70kDa, polypeptide 1.
2	9756	Hs.446352	V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian).
2	9756	Hs.436405	Isocitrate dehydrogenase 3 (NAD+) beta.
2	9756	Hs.370725	Oxysterol binding protein-like 1A.
2	9756	Hs.369144	Achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A).
2	9756	Hs.195464	Filamin A, alpha (actin binding protein 280).
2	9756	Hs.113157	Integrin, alpha 9.
1	4878	Hs.75724	Coatomer protein complex, subunit beta 2 (beta prime).
1	4878	Hs.74050	3-ketodihydrosphingosine reductase.
1	4878	Hs.718675	Transcribed locus, strongly similar to NP_055901.1 dendrin [Homo sapiens].
1	4878	Hs.716474	CCR4-NOT transcription complex, subunit 1.
1	4878	Hs.716465	Transcribed locus, weakly similar to XP_001154052.1 PREDICTED: hypothetical protein, partial [Pan troglodytes].
1	4878	Hs.715771	Transcribed locus.
1	4878	Hs.715543	BRCA2 and CDKN1A interacting protein.
1	4878	Hs.715537	Transducin (beta)-like 1 X-linked receptor 1.
1	4878	Hs.708847	MRNA; cDNA DKFZp686D10250 (from clone DKFZp686D10250).
1	4878	Hs.706828	Lon peptidase 2, peroxisomal.
1	4878	Hs.689114	MRNA; cDNA DKFZp686N1898 (from clone DKFZp686N1898).
1	4878	Hs.676173	Transcribed locus.
1	4878	Hs.669952	MRNA; cDNA DKFZp586A2322 (from clone DKFZp586A2322).
1	4878	Hs.669715	Transcribed locus, moderately similar to NP_989488.2 eukaryotic translation elongation factor 1 alpha 1 [Gallus gallus].
1	4878	Hs.668781	Transcribed locus.
1	4878	Hs.666082	Transcribed locus.
1	4878	Hs.665043	Transcribed locus.
1	4878	Hs.662428	Transcribed locus.
1	4878	Hs.660415	CDNA FLJ39766 fis, clone SPLEN2000307.
1	4878	Hs.658983	Transcribed locus.
1	4878	Hs.658797	Transcribed locus.
1	4878	Hs.657275	Hypothetical transcript.
1	4878	Hs.656970	Transcribed locus.
1	4878	Hs.656668	Hypothetical protein LOC642031.
1	4878	Hs.655536	Zinc finger and BTB domain containing 1.
1	4878	Hs.652255	KIAA0409.
1	4878	Hs.648439	Hypothetical LOC100190986.
1	4878	Hs.645450	Similar to mCG18564.
1	4878	Hs.644639	Eukaryotic translation elongation factor 1 alpha 1.
1	4878	Hs.643498	Chromosome 6 open reading frame 106.
1	4878	Hs.635	Calcium channel, voltage-dependent, beta 1 subunit.
1	4878	Hs.632181	Spinster homolog 1 (Drosophila).
1	4878	Hs.631618	Tropomyosin 4.
1	4878	Hs.629221	Transcribed locus, strongly similar to NP_036524.1 penta-EF-hand domain containing 1 [Homo sapiens].
1	4878	Hs.624193	Transcribed locus.
1	4878	Hs.610337	Transcribed locus.
1	4878	Hs.604001	Heterogeneous nuclear ribonucleoprotein H1 (H).
1	4878	Hs.595158	Zinc finger, RAN-binding domain containing 1.
1	4878	Hs.592174	Transmembrane protein 184A.
1	4878	Hs.584775	Low density lipoprotein receptor-related protein 6.
1	4878	Hs.560343	RAR-related orphan receptor A.
1	4878	Hs.555970	Small nuclear ribonucleoprotein polypeptide N.
1	4878	Hs.532363	Homeodomain interacting protein kinase 1.
1	4878	Hs.529451	Diaphanous homolog 1 (Drosophila).
1	4878	Hs.524731	Potassium channel tetramerisation domain containing 10.
1	4878	Hs.524518	Signal transducer and activator of transcription 6, interleukin-4 induced.
1	4878	Hs.519523	Serpin peptidase inhibitor, clade B (ovalbumin), member 6.
1	4878	Hs.515271	Splicing factor, arginine/serine-rich 14.
1	4878	Hs.507087	Signal peptide peptidase 3.
1	4878	Hs.506985	WD repeat and SOCS box-containing 2.
1	4878	Hs.502756	AHNAK nucleoprotein.
1	4878	Hs.501794	ATPase type 13A1.
1	4878	Hs.497589	Polymeric immunoglobulin receptor.
1	4878	Hs.497118	Cytochrome c oxidase subunit VIa polypeptide 1.
1	4878	Hs.493164	Nicotinate phosphoribosyltransferase domain containing 1.
1	4878	Hs.491060	Ash1 (absent, small, or homeotic)-like (Drosophila).
1	4878	Hs.4779	GATA zinc finger domain containing 2B.
1	4878	Hs.474938	Solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17.
1	4878	Hs.471779	Leucine rich repeat (in FLII) interacting protein 1.
1	4878	Hs.471452	Phenylalanyl-tRNA synthetase, beta subunit.
1	4878	Hs.469970	Splicing factor, arginine/serine-rich 4.
1	4878	Hs.467807	Lysosomal protein transmembrane 4 alpha.
1	4878	Hs.465885	Interleukin enhancer binding factor 3, 90kDa.
1	4878	Hs.462971	Per1-like domain containing 1.
1	4878	Hs.456557	Ring finger protein 220.
1	4878	Hs.449942	Inositol polyphosphate-5-phosphatase, 75kDa.
1	4878	Hs.446240	Zinc finger, MYND-type containing 8.
1	4878	Hs.443134	Glucosidase, beta (bile acid) 2.
1	4878	Hs.440384	Zinc finger protein 789.