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Library:16454 (dbEST ID)
PT-P-FL-A-2
Library Description
| Organism: | Populus trichocarpa |
| Cultivar: | 383-2499 (Nisqually-1) |
| Sex: | Female |
| Vector: | pBluescript II SK (+) XR |
| Cloning site 1: | SstI (5' end of cDNA) |
| Cloning site 2: | XhoI (3' end of cDNA) |
| Lab host: | E. coli DH10B T1 phage resistant cells |
Phloem and cambium from 8 year old trees harvested within the Boise Cascade region of Washington state on May 15th, 2001. cDNA was prepared from 20 micrograms of mRNA according to the full-length cDNA library construction method described by Carninci P. et al. (2000), Genome Research 10(10):1617-1630 and directionally ligated into the pBluescript II SK (+) XR vector digested with SstI (5' end) and XhoI (3'). Plasmid DNA was then transformed by electroporation into DH10B cells (Invitrogen) for propagation.
Source: Joerg Bohlmann, University of British Columbia
Gene Content Analysis
12,201 ESTs from this library were grouped into 2,782 UniGene entries (putative genes) [UniGene build #9, 28-May-2008]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).
| ESTs | TPM | UniGene Entry | ||
|---|---|---|---|---|
| 98 | 8032 |  |
Pth.16385 | Clone WS01111_N04 unknown mRNA. |
| 89 | 7294 | |
Pth.16455 | Clone PX0019_B24 unknown mRNA. |
| 83 | 6803 | |
Pth.16373 | Clone WS01212_N14 unknown mRNA. |
| 71 | 5819 |  |
Pth.3324 | Clone WS01120_G22 unknown mRNA. |
| 70 | 5737 | |
Pth.4769 | Clone WS01118_K20 unknown mRNA. |
| 70 | 5737 | |
Pth.16396 | Clone WS01118_J09 unknown mRNA. |
| 67 | 5491 | |
Pth.4327 | Clone PX0019_E08 unknown mRNA. |
| 66 | 5409 | |
Pth.16761 | Clone WS01110_E02 unknown mRNA. |
| 60 | 4918 | |
Pth.3837 | Clone WS0115_E11 unknown mRNA. |
| 56 | 4590 |  |
Pth.2583 | Clone WS0111_G06 unknown mRNA. |
| 55 | 4508 | |
Pth.16728 | Clone WS01119_O18 unknown mRNA. |
| 53 | 4344 | |
Pth.16548 | Clone WS01120_N23 unknown mRNA. |
| 51 | 4180 | |
Pth.3017 | Clone PX0011_J01 unknown mRNA. |
| 50 | 4098 | |
Pth.1614 | Clone WS01118_D16 unknown mRNA. |
| 49 | 4016 | |
Pth.4877 | Clone WS01118_B19 unknown mRNA. |
| 49 | 4016 | |
Pth.3233 | Clone WS01118_E04 unknown mRNA. |
| 49 | 4016 | |
Pth.16516 | Clone PX0019_I16 unknown mRNA. |
| 46 | 3770 | |
Pth.16723 | Clone WS01119_L18 unknown mRNA. |
| 46 | 3770 | |
Pth.16374 | Clone WS0111_B09 unknown mRNA. |
| 44 | 3606 | |
Pth.9090 | Clone WS01123_J13 unknown mRNA. |
| 43 | 3524 |  |
Pth.16545 | Clone PX0019_K22 unknown mRNA. |
| 41 | 3360 | |
Pth.10921 | Clone WS0111_C12 unknown mRNA. |
| 39 | 3196 | |
Pth.4331 | Clone WS01117_J14 unknown mRNA. |
| 39 | 3196 | |
Pth.3979 | Clone WS01122_E19 unknown mRNA. |
| 38 | 3114 | |
Pth.673 | Clone PX0019_E04 unknown mRNA. |
| 38 | 3114 | |
Pth.16403 | Clone PX0015_K11 unknown mRNA. |
| 37 | 3033 | |
Pth.3969 | Clone PX0019_F04 unknown mRNA. |
| 36 | 2951 | |
Pth.3154 | Clone WS01118_G14 unknown mRNA. |
| 36 | 2951 | |
Pth.16506 | Clone PX0019_K15 unknown mRNA. |
| 35 | 2869 | |
Pth.2865 | Clone WS0116_C19 unknown mRNA. |
| 34 | 2787 | |
Pth.4560 | Clone WS0112_D05 unknown mRNA. |
| 34 | 2787 | |
Pth.4049 | Clone PX0019_C18 unknown mRNA. |
| 34 | 2787 | |
Pth.3924 | Clone WS01214_J23 unknown mRNA. |
| 34 | 2787 | |
Pth.3747 | Clone PX0019_M02 unknown mRNA. |
| 33 | 2705 | |
Pth.3293 | Clone PX0019_I20 unknown mRNA. |
| 33 | 2705 | |
Pth.16378 | Clone WS0113_H11 unknown mRNA. |
| 33 | 2705 | |
Pth.11269 | Clone WS01124_F24 unknown mRNA. |
| 32 | 2623 | |
Pth.5101 | Clone WS01121_I05 unknown mRNA. |
| 32 | 2623 | |
Pth.16446 | Clone WS0111_N01 unknown mRNA. |
| 32 | 2623 | |
Pth.16435 | Clone WS0113_E20 unknown mRNA. |
| 31 | 2541 | |
Pth.3205 | Clone WS01121_G12 unknown mRNA. |
| 31 | 2541 | |
Pth.16876 | Clone WS0111_C11 unknown mRNA. |
| 31 | 2541 | |
Pth.16443 | Clone WS0113_G06 unknown mRNA. |
| 30 | 2459 |  |
Pth.4704 | Clone WS01118_K13 unknown mRNA. |
| 30 | 2459 | |
Pth.16464 | Clone WS01124_I17 unknown mRNA. |
| 28 | 2295 | |
Pth.4302 | Clone WS0118_F13 unknown mRNA. |
| 27 | 2213 | |
Pth.16518 | Clone WS01122_B06 unknown mRNA. |
| 26 | 2131 | |
Pth.4424 | Clone WS01121_I06 unknown mRNA. |
| 26 | 2131 | |
Pth.3651 | Clone WS0116_E12 unknown mRNA. |
| 26 | 2131 | |
Pth.2905 | Clone WS0112_G15 unknown mRNA. |
| 26 | 2131 | |
Pth.16689 | Clone WS01122_L24 unknown mRNA. |
| 25 | 2049 | |
Pth.4318 | Clone WS01118_K09 unknown mRNA. |
| 25 | 2049 | |
Pth.16532 | Clone PX0019_B16 unknown mRNA. |
| 25 | 2049 | |
Pth.16405 | Clone WS0111_E04 unknown mRNA. |
| 24 | 1967 | |
Pth.547 | Clone WS01123_B04 unknown mRNA. |
| 24 | 1967 | |
Pth.2149 | Clone WS0115_B08 unknown mRNA. |
| 24 | 1967 | |
Pth.11437 | Clone WS01125_O15 unknown mRNA. |
| 23 | 1885 | |
Pth.8994 | Clone WS01118_I19 unknown mRNA. |
| 23 | 1885 | |
Pth.5278 | Clone WS01117_K13 unknown mRNA. |
| 23 | 1885 | |
Pth.4671 | Clone WS01119_C17 unknown mRNA. |
| 23 | 1885 | |
Pth.4497 | Clone WS0113_B18 unknown mRNA. |
| 23 | 1885 | |
Pth.4033 | Clone WS0115_O15 unknown mRNA. |
| 23 | 1885 | |
Pth.16634 | Clone WS01120_C03 unknown mRNA. |
| 22 | 1803 | |
Pth.2939 | Clone WS0118_E06 unknown mRNA. |
| 22 | 1803 | |
Pth.284 | Clone WS01122_M11 unknown mRNA. |
| 22 | 1803 | |
Pth.16509 | Clone PX0019_P07 unknown mRNA. |
| 22 | 1803 | |
Pth.16382 | Clone WS0116_D18 unknown mRNA. |
| 22 | 1803 | |
Pth.1251 | Clone WS01117_E16 unknown mRNA. |
| 22 | 1803 | |
Pth.11428 | Clone WS0116_F12. |
| 21 | 1721 | |
Pth.8462 | Clone WS01126_L07 unknown mRNA. |
| 21 | 1721 | |
Pth.4338 | Clone PX0019_F23 unknown mRNA. |
| 21 | 1721 | |
Pth.3419 | Clone WS01119_N18 unknown mRNA. |
| 21 | 1721 | |
Pth.11463 | Clone WS0112_E04 unknown mRNA. |
| 20 | 1639 | |
Pth.4275 | Clone PX0019_G20 unknown mRNA. |
| 20 | 1639 | |
Pth.4072 | Clone WS0117_K20 unknown mRNA. |
| 20 | 1639 | |
Pth.3770 | Clone WS0124_H23 unknown mRNA. |
| 20 | 1639 | |
Pth.3275 | Clone WS0112_A17 unknown mRNA. |
| 20 | 1639 | |
Pth.16497 | Clone WS0112_L21 unknown mRNA. |
| 20 | 1639 | |
Pth.16418 | Clone WS01118_E17 unknown mRNA. |
| 20 | 1639 | |
Pth.16368 | Clone WS01122_J20 unknown mRNA. |
| 19 | 1557 |  |
Pth.4599 | Clone WS0113_M03 unknown mRNA. |
| 19 | 1557 | |
Pth.16618 | Clone WS0111_I06 unknown mRNA. |
| 19 | 1557 | |
Pth.11469 | Clone PX0015_O07 unknown mRNA. |
| 18 | 1475 | |
Pth.712 | Clone PX0019_F02 unknown mRNA. |
| 18 | 1475 | |
Pth.7112 | Clone WS01125_J01 unknown mRNA. |
| 18 | 1475 | |
Pth.4447 | Clone WS0113_J23 unknown mRNA. |
| 18 | 1475 | |
Pth.4317 | Clone WS0111_A19 unknown mRNA. |
| 18 | 1475 | |
Pth.3879 | Clone WS01111_O02 unknown mRNA. |
| 18 | 1475 | |
Pth.2995 | Clone WS0113_D21 unknown mRNA. |
| 18 | 1475 | |
Pth.272 | Clone WS01125_J22 unknown mRNA. |
| 18 | 1475 | |
Pth.2610 | Clone WS0114_O11 unknown mRNA. |
| 18 | 1475 | |
Pth.16617 | Clone WS0115_M01 unknown mRNA. |
| 18 | 1475 | |
Pth.16468 | Clone WS0118_J13 unknown mRNA. |
| 18 | 1475 | |
Pth.16465 | Clone WS01119_A24 unknown mRNA. |
| 18 | 1475 | |
Pth.16371 | Clone WS01111_H21 unknown mRNA. |
| 18 | 1475 | |
Pth.12 | Clone WS01111_A23 unknown mRNA. |
| 18 | 1475 | |
Pth.11447 | Clone WS01123_A22 unknown mRNA. |
| 18 | 1475 | |
Pth.10900 | Clone PX0015_O12 unknown mRNA. |
| 18 | 1475 | |
Pth.10661 | Clone WS01124_I16 unknown mRNA. |
| 17 | 1393 | |
Pth.9318 | Clone WS0114_J22 unknown mRNA. |
Source: NCBI UniGene
EST Sequences
12,929 EST sequences from this library have been submitted to the public sequence database as of 28-May-2008.
Source: NCBI GenBank
Click the button to save sequences to a file (FASTA format)
Keywords
- stem
- sapling
- female
- enriched
- directionally cloned
Source: NCBI Clone Registry
References
Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar. Ralph S, et al., Mol Ecol 15, 1275-1297 (2006).