|
Status |
Public on Dec 01, 2006 |
Title |
chorion villus cells_normalkaryotype_rep1 |
Sample type |
RNA |
|
|
Source Name |
chorion villus cells, normal karyotype
|
Organism(s) |
Homo sapiens |
Characteristics |
tissue: prenatal chorion villus cells Age: 12 gestational weeks gender: female karyotype: normal
|
Biomaterial provider |
Department of Medical Genetics of University of Tuebingen, Germany
|
Treatment protocol |
No treatment
|
Growth protocol |
Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
|
Label |
biotin
|
Label protocol |
Affymetrix standard labeling protocol
|
|
|
Hybridization protocol |
Affymetrix standard hybridization protocol
|
Scan protocol |
Affymetrix standard scan protocol
|
Description |
chorion villus cells euploid control sample replicate 1
|
Data processing |
Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
|
|
|
Submission date |
Nov 09, 2006 |
Contact name |
Oezge Altug Teber |
E-mail(s) |
oezge.altug-teber@med.uni-tuebingen.de
|
Phone |
004970712972307
|
Fax |
00497071295172
|
Organization name |
University of Tuebingen
|
Department |
Medical Genetics
|
Lab |
Microarray Facility
|
Street address |
Calwerstr. 7
|
City |
Tuebingen |
ZIP/Postal code |
72076 |
Country |
Germany |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE6283 |
Specific transcriptional changes in human fetus with autosomal trisomies |
|