|
| Status |
Public on Dec 01, 2006 |
| Title |
chorion villus cells_normalkaryotype_rep1 |
| Sample type |
RNA |
| |
|
| Source Name |
chorion villus cells, normal karyotype
|
| Organism(s) |
Homo sapiens |
| Characteristics |
tissue: prenatal chorion villus cells
Age: 12 gestational weeks
gender: female
karyotype: normal
|
| Biomaterial provider |
Department of Medical Genetics of University of Tuebingen, Germany
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Primary cell cultures of the chorion villus cells were performed using slide flasks (with NunclonTM surface, 9 cm2, NUNCTM). Chorionic villi were grown in Amnio Max C100 Basal Medium and Amnio Max C100 Supplement (Invitrogen) at 37°C in 5% CO2. The second successive subculture was set up in culture flasks (with CELLSTAR® surface, 25 cm2, Greiner bio-one) using the same medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested by centrifugation and media was removed by suction. The cells were then resuspended in RLT-buffer provided in RNeasy mini kit (QIAGEN) and total RNA was isolated from each cell culture using RNeasy mini kit according to manufacturer’s instructions (QIAGEN).
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard labeling protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard hybridization protocol
|
| Scan protocol |
Affymetrix standard scan protocol
|
| Description |
chorion villus cells euploid control sample replicate 1
|
| Data processing |
Call detection and signal intensities were generated by Affymetrix GCOS software. Raw data were imorted into ArrayAssist version 4.1 software. For normalization, GC-RMA normalization algorithm implemented in ArrayAssist were applied.
|
| |
|
| Submission date |
Nov 09, 2006 |
| Contact name |
Oezge Altug Teber |
| E-mail(s) |
oezge.altug-teber@med.uni-tuebingen.de
|
| Phone |
004970712972307
|
| Fax |
00497071295172
|
| Organization name |
University of Tuebingen
|
| Department |
Medical Genetics
|
| Lab |
Microarray Facility
|
| Street address |
Calwerstr. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6283 |
Specific transcriptional changes in human fetus with autosomal trisomies |
|
